Objective:To investigate the expressions of P27kipl (P27) protein and cyclin E protein in human renal cell carcinoma and in paracarcinoma kidney cortex tissue,and to explore the correlation of these protein expressions with biological behavior of tumors.Methods:P27 and cyclin E expressions were studied in formalin-fixed,paraffin-embedded sections of renal cell carcinoma and in paracarcinoma kidney cortex tissue by means of a specific and sensitive immunohistochemical S-P assay.Results:The positive rates of P27 protein and cyclin E protein in renal cell carcinoma were significantly higher than those in paracarcinoma kidney cortex tissues ( P

Objective To evaluate the safety and feasibility of minimally invasive percutaneous nephrolithotomy (mPCNL) for the treatment of upper urinary tract calculi. Methods From October 2003 to December 2006, mPCNL was performed on 127 patients suffering from upper urinary tract calculi by using Karl Storz F8/9.8 rigid ureteroscope and homemade pneumatic ballistic lithotriptor. Results Of the 127 cases, mPCNL was completed in 125, and was converted to open surgery in 2. The rate of stone removal was 89.8% (114/127). The operation time was 78-185 min (mean 108 min). Nephrostomy tube was kept for a mean of 7 d (5-11 d). The mean hospital stay was 8.1 d (5-12 d). Two patients received blood transfusion during the operation. One patient developed massive hemorrhage at the nephrostomy opening, and was cured by high-selective renal artery embolization. Among the patients, 117 were followed up for 1-16 months (mean 5.3), during which no recurrent urinary tract stones was found by B-ultrasonography or X-ray. Conclusions By using mPCNL, patients with upper urinary tract calculi can be treated safely and effectively with a high rate of stone removal, less trauma, quick recovery, and short hospital stay.

Objective To supervise the colonoscopy following up in the high risk colorectal cancer patients are good to find out early stage cancer and adenoma in time and prevent the latter to evolve in to cancer. Methods From Aug. 1995 to Mar. 2000, 1 125 cases of high risk colorectal cancer have received the colonoscopy .Of them, 353 cases who have symptoms after having the physical examination in clinics; 125 cases have the colorectal cancer family history without any symptoms; 386 cases have had operated on the colorectal cancer; and 261 cases with the colorectal adenoma after high frequency electrotomy under endoscopy in one year. Results In 353 cases, 52 cases (14.7%) with adenoma and 17 cases (4.8%) with colorectal cancer are found, and 12 cases (70.5%)of the latter are of early stage cancer without any lymph node metastasis. Among the 125 cases there were 26 cases (21.6%) with adenoma, 4 cases (3.2%) with colorectal cancer and 2 cases (1.6%) with adenoma early stage cancer. Among the latter 6 cases, 5 cases are of the early stage cancer without any lymph node metastasis. Of the 386 cases , 81 cases (20.9%) of adenoma, 3 cases (0.7%) of the third primary cancer, and 11 cases (2.8%) of second primary cancer were found in which 11 cases (78.5%) are of the early stage cancer without any lymph node metastasis. In 261 cases there were 56 cases (21.4%) with adenoma, among them, 41 cases (73.2%) are the newly found adenoma with diameter 1cm in diameter were missed in the first colonoscopy.Conclusion A total of 1 125 cases with high risk colorectal cancer were examined by colonoscopy, it was found 37 cases (3.3%) with carcinoma, in which 28 cases (75.7%) with early cancer of no lymph node metastasis, and other 215 cases (19.1%) with adenoma.

Blood Banks ; Cord Blood Stem Cell Transplantation ; Fetal Blood ; Humans

Accidents, Occupational ; Acetic Anhydrides ; Adult ; Blast Injuries ; complications ; therapy ; Burns, Inhalation ; etiology ; therapy ; Humans ; Lung Injury ; etiology ; therapy ; Male ; Middle Aged

Objective To investigate the efficacy of clinical case-based PBL in clinical practice in department of urology.Methods Medical students admitted by our clinical medical school in 2006 and 2007 were involved.Fifty-six students of 2007 grade were chosen as study group while 49students of 2006 grade as control group.Students in study group were taught by clinical case-based PBL method while those in control group by traditional method.All results were analyzed by SPSS 11.0.Results The scores in study group were higher than those in control group with statistical differences (82.84 ± 12.35 vs.72.95 ± 11.13 ; t =4.29,P ＜ 0.01).Students in study group showed more interests in practice lessons.Teachers were more satisfied with the overall performance of students in study group.Conclusions Clinical case-based PBL can increase students' overall performance such as better coordination,better clinical skills and better open-minded clinical case solution.

Objective To study the expressions of p27Kip1 (p27) protein and Ki 67 antigen in human clear cell carcinoma of kidney and to find out the relationship between the expression levels and the clinical pathological characteristics and prognosis in cases of clear cell carcinoma of kidney. Methods The expressions of p27 protein and Ki 67 antigen in the adjacent tissues of carcinoma in 20 cases and 42 cases of clear cell carcinoma of kidney were detected by immunohistochemistry. Results The positive rates of p27 protein and Ki 67 antigen in clear cell carcinoma of kidney were significantly higher than those of the both in the adjacent tissues of carcinoma( P

Objective:To study adenovirus mediated transfer of TGF ?1 in treatment of mice autoimmune myocarditis. Methods: Experimental autoimmune myocarditis was induced in BALB/c mice using purified myosin from porcine heart. TGF ?1 was transferred into mice by intravenous injection of adenovirus encoding TGF ?1. Plasma level of myosin autoantibody was detected by indirect ELISA method and plasma level of troponin I was assayed using fluorescence immune assay method. The protective effects of TGF ?1 were evaluated from 3 aspects: (1) the degree of infiltrative inflammatory cells in the heart; (2) the plasma level of myosin autoantibody; (3) the plasma level of troponin I. Results: TGF ?1 successfully ameliorated the myocarditis. Plasma level of troponin I and myosin autoantibody decreased significantly( P

Objective To investigate the nutrition support during stress response period after tumor ex-tirpation in middle-aged and elderly patients with oral and maxillofacial tumors. Methods Sixty patients who had undergone maxillofacial tumor extirpation were randomly divided into nutrition therapy group (n = 35) and control group ( n = 25 ). Patients in nutrition therapy group received enteral nutritional treatment, while patients in control group fed themselves. The nutritional status of the two groups before operations were evaluated with Concise Nutritional Assessment Indicators of human body measurement and biochemistry were used to evaluate the nutritional conditions. The incidence of post-operational complications and recovery time ( from the operation to discharge) were analyzed. Results The weight and arm muscle circumference in the nutrition therapy group significantly decreased after operations (P <0. 05). However, in the control group, all the nutritional indica-tors except the total lymphocyte count were significantly lower than the baseline levels after operations (P < 0. 05 ) ; particularly, of the average body weight decreased by about 6 kg. The incidence of complications in the nutrition therapy group was significantly lower than that in control group (5. 7% vs. 12. 0% , P <0. 05). The recovery time was significantly longer in the control group than that in the nutrition therapy group [ (28. 62± 2.38) vs. (22. 76± 3. 24) d, P < 0. 01 ]. Conclusion The nutritional support during the stress response period can satisfy the demands of high catabolism after maxillofacial tumor extirpation operation in middle-aged and elderly patients.

Objective To develop clinical dual-qualification nursing teachers stressors scale. Methods Based on literature review, semi-structured interview and the Delphi, first of all, the item pool was made up, then the pre-test scale was formatted. 167 dual-qualification nursing teachers participated in this investigation.Finally, the official scale was formed after measuring its reliability and validity. Results The scale was made up of six dimensions and 25 items. The Cronbach's α was 0.652 ～ 0.903, and the intraclass correlation coefficient was 0.716 ～ 0.921. Conclusion The process of this scale is scientific, rational, rigorous. The scale has good psychometric characteristics and can be used for the stress assessment of dual-qualification nursing teachers.