Objective:To investigate the expressions of P27kipl (P27) protein and cyclin E protein in human renal cell carcinoma and in paracarcinoma kidney cortex tissue,and to explore the correlation of these protein expressions with biological behavior of tumors.Methods:P27 and cyclin E expressions were studied in formalin-fixed,paraffin-embedded sections of renal cell carcinoma and in paracarcinoma kidney cortex tissue by means of a specific and sensitive immunohistochemical S-P assay.Results:The positive rates of P27 protein and cyclin E protein in renal cell carcinoma were significantly higher than those in paracarcinoma kidney cortex tissues ( P

Objective To evaluate the safety and feasibility of minimally invasive percutaneous nephrolithotomy (mPCNL) for the treatment of upper urinary tract calculi. Methods From October 2003 to December 2006, mPCNL was performed on 127 patients suffering from upper urinary tract calculi by using Karl Storz F8/9.8 rigid ureteroscope and homemade pneumatic ballistic lithotriptor. Results Of the 127 cases, mPCNL was completed in 125, and was converted to open surgery in 2. The rate of stone removal was 89.8% (114/127). The operation time was 78-185 min (mean 108 min). Nephrostomy tube was kept for a mean of 7 d (5-11 d). The mean hospital stay was 8.1 d (5-12 d). Two patients received blood transfusion during the operation. One patient developed massive hemorrhage at the nephrostomy opening, and was cured by high-selective renal artery embolization. Among the patients, 117 were followed up for 1-16 months (mean 5.3), during which no recurrent urinary tract stones was found by B-ultrasonography or X-ray. Conclusions By using mPCNL, patients with upper urinary tract calculi can be treated safely and effectively with a high rate of stone removal, less trauma, quick recovery, and short hospital stay.

Objective To supervise the colonoscopy following up in the high risk colorectal cancer patients are good to find out early stage cancer and adenoma in time and prevent the latter to evolve in to cancer. Methods From Aug. 1995 to Mar. 2000, 1 125 cases of high risk colorectal cancer have received the colonoscopy .Of them, 353 cases who have symptoms after having the physical examination in clinics; 125 cases have the colorectal cancer family history without any symptoms; 386 cases have had operated on the colorectal cancer; and 261 cases with the colorectal adenoma after high frequency electrotomy under endoscopy in one year. Results In 353 cases, 52 cases (14.7%) with adenoma and 17 cases (4.8%) with colorectal cancer are found, and 12 cases (70.5%)of the latter are of early stage cancer without any lymph node metastasis. Among the 125 cases there were 26 cases (21.6%) with adenoma, 4 cases (3.2%) with colorectal cancer and 2 cases (1.6%) with adenoma early stage cancer. Among the latter 6 cases, 5 cases are of the early stage cancer without any lymph node metastasis. Of the 386 cases , 81 cases (20.9%) of adenoma, 3 cases (0.7%) of the third primary cancer, and 11 cases (2.8%) of second primary cancer were found in which 11 cases (78.5%) are of the early stage cancer without any lymph node metastasis. In 261 cases there were 56 cases (21.4%) with adenoma, among them, 41 cases (73.2%) are the newly found adenoma with diameter 1cm in diameter were missed in the first colonoscopy.Conclusion A total of 1 125 cases with high risk colorectal cancer were examined by colonoscopy, it was found 37 cases (3.3%) with carcinoma, in which 28 cases (75.7%) with early cancer of no lymph node metastasis, and other 215 cases (19.1%) with adenoma.

Objective To probe into the status quo of maltreatment tendency among family caregivers of elderly patients with chronic diseases and its influence factors.Methods Totally 402 family caregivers of the elderly patients with chronic diseases were selected and surveyed by using the Chinese version of caregiver elder abuse assessment scale and the results underwent statistical analysis.Results 63.70％ of family caregivers in elderly patients with chronic diseases had the elder abuse risk tendency and its influence factors were involved with both aspects of family caregivers and the care recipients,including the gender,cultural level and self-care ability of daily living in the elderly patients with chronic diseases and the age,cultural level,care period and living together with elderly in caregivers.Conclusion The elder abuse problem in the patients with chronic diseases should be paid more attention to,which needs the collaboration of multidisciplinary team,various effective ways should be adopted to relieve the family caregivers' pressure for reducing the occurrenc of elder abuse risk.

Objective To evaluate the effect of group reminiscence therapy on improving the depressive symptoms of the institutionalized elderly. Methods Collecting clinical randomized controlled trials and quasi-randomized controlled trials of group reminiscence therapy for improving depressive symptoms in institutionalized elderly which published from 2000 to November 2016, by searching the Cochrane Library, PubMed, CINAHL, PsycINFO, EMbase, China Biology Medicine, China National Knowledge Infrastructure, VIP, Wanfang Database, Taiwan Academic Online Database and Airiti Library. And reviewing the quality of the studies by using the tools for assessing risk of bias. RevMan 5.3 software was used for data analysis. Results Ten studies(2 randomized controlled trials and 8 quasi-experiments), including a total of 511 cases, were included. The results showed that group reminiscence therapy significantly improved the depression, life satisfaction, and self-esteem of institutionalized elderly (95%CI-1.11--0.56, 0.41-2.05, 1.99-3.82, all P<0.01). There was no statistically significant difference between group reminiscence therapy and usual nursing in relieving loneliness (P = 0.21). Conclusions Group reminiscence therapy can improve the depression in institutionalized elderly, decrease the degree of depression, improve the level of life satisfaction and self-esteem. But in the ease of loneliness has not been confirmed. The randomized controlled trials included in this study were rare and the quality of the literature was low, and large-scale randomized controlled trials of higher quality are needed to confirm this.

Objective To investigate the nutrition support during stress response period after tumor ex-tirpation in middle-aged and elderly patients with oral and maxillofacial tumors. Methods Sixty patients who had undergone maxillofacial tumor extirpation were randomly divided into nutrition therapy group (n = 35) and control group ( n = 25 ). Patients in nutrition therapy group received enteral nutritional treatment, while patients in control group fed themselves. The nutritional status of the two groups before operations were evaluated with Concise Nutritional Assessment Indicators of human body measurement and biochemistry were used to evaluate the nutritional conditions. The incidence of post-operational complications and recovery time ( from the operation to discharge) were analyzed. Results The weight and arm muscle circumference in the nutrition therapy group significantly decreased after operations (P <0. 05). However, in the control group, all the nutritional indica-tors except the total lymphocyte count were significantly lower than the baseline levels after operations (P < 0. 05 ) ; particularly, of the average body weight decreased by about 6 kg. The incidence of complications in the nutrition therapy group was significantly lower than that in control group (5. 7% vs. 12. 0% , P <0. 05). The recovery time was significantly longer in the control group than that in the nutrition therapy group [ (28. 62± 2.38) vs. (22. 76± 3. 24) d, P < 0. 01 ]. Conclusion The nutritional support during the stress response period can satisfy the demands of high catabolism after maxillofacial tumor extirpation operation in middle-aged and elderly patients.

Objective To develop clinical dual-qualification nursing teachers stressors scale. Methods Based on literature review, semi-structured interview and the Delphi, first of all, the item pool was made up, then the pre-test scale was formatted. 167 dual-qualification nursing teachers participated in this investigation.Finally, the official scale was formed after measuring its reliability and validity. Results The scale was made up of six dimensions and 25 items. The Cronbach's α was 0.652 ～ 0.903, and the intraclass correlation coefficient was 0.716 ～ 0.921. Conclusion The process of this scale is scientific, rational, rigorous. The scale has good psychometric characteristics and can be used for the stress assessment of dual-qualification nursing teachers.

Objective:To study adenovirus mediated transfer of TGF ?1 in treatment of mice autoimmune myocarditis. Methods: Experimental autoimmune myocarditis was induced in BALB/c mice using purified myosin from porcine heart. TGF ?1 was transferred into mice by intravenous injection of adenovirus encoding TGF ?1. Plasma level of myosin autoantibody was detected by indirect ELISA method and plasma level of troponin I was assayed using fluorescence immune assay method. The protective effects of TGF ?1 were evaluated from 3 aspects: (1) the degree of infiltrative inflammatory cells in the heart; (2) the plasma level of myosin autoantibody; (3) the plasma level of troponin I. Results: TGF ?1 successfully ameliorated the myocarditis. Plasma level of troponin I and myosin autoantibody decreased significantly( P

Objective To provide anatomical basis for one-stage full dynamic correction of late facial palsy with double latissimus dorsi segmental muscle flaps.MethodsForty sides of formaldehyde fixed latissimus dorsi in twenty cadavers were selected to microsurgery anatomy,three sides fresh latissimus dorsi specimens were blood vessel casted.The extramusclar and intramuscular neurovascular distribution were observed.Results①In 92.5%of the thoracodorsal nerve divides into a medial and a lateral branch be fore entering the muscle.In 7.5%of the thoracodorsal nerve divides into three major branches.The coordinate of the bifurcation point of the thoracodorsal Here was[(7.94±1.23)cm,(3.71±1.68)cm].In the district of this angle's middle line,the numbers of vessels and nerves is relative few.②The lateral muscle flaps of the latissimus dorsi can be divided into 3-5 independent segmental flaps,the medial muscle flaps of the latissimus dorsi Can be divided into 2-4 independent segmental flaps.③The segmental nerve usually locate the two sides of vessel,which are arranged(from medial to lateral)in a NVAV order in the reedial segmental branches(100%),in a VAVN order in the lateral segmental branches(85.0%)and in a NVAV order in the lateral segmental branches(15.0%).④The neural pedicle Was cut from the lateral bifurcation point,the mean length of the third medial muscle neural pedicle was 16 cm；the mean length of the third or the forth lateral muscle neural pedicle was 12 cm.Conclusion Late facial palsy can be cuwd with onestage cross-facial transplantation of neurovascularized free double latissimus dorsi segmental muscle flaps.

Integrin ?V?3, one of the major members of the integrin family, plays a pivotal role in tumor angiogenesis because it mediates the cell-extracellular matrix and cell to cell adhesion as well as controls cells proliferation, differentiation, cellular modality and motility.Due to restricted high-expressions in tumors, integrin ?V?3, is considered a suitable targeting receptor for tumor diagnosis.In the past decade, several molecular imaging tracers for integrin ?V?3, have been used to noninvasively visualize its expression in tumor tissues and angiogenic blood vessels through the usage of nuclear imaging, nuclear magnetic resonance imaging, ultrasound, and optical imaging.Noninvasive detection of integrin ?V?3, expression can potentially be used for tumor diagnosis and prognosis.The current imaging probes used to target integrin ?V?3, through usage of different technologies are briefly recapitulated in this review.