Objective To investigate the differential expression of synaptophysin(SYN)in the developing rat cochlea,to analyze the relationship between the expression of SYN and auditory functional development,and to explore the source of the adenosine triphosphate(ATP)in the cochlea.Methods The immunofluorescence was applied to observe the differential expression of SYN in the cochlea of SD rats on different days(p1,p5,p10,p14 and p28)after parturition.Results No significant stain was observed in the Kolliker organ or the Corti's organ of P1,P5 and the top turn of the cochlea in P10.The SYN expression was turned on the outer spiral bundle,the inner spiral bundle,and the medial wall of the Deiters' cell of the cochlea in P14,P28 and the second or the third turn of Corti's organ of P10.All the spiral ganglion neurons(SGN)were stained in different aged cochlea.Conclusion The expression of SYN was different during development of rat cochlea.This difference was in favor of the configuration constructed between nerve endings and target cells.It also played a key role in the formation of correct auditory coding during auditory system development.The hair cells and the supporting cells,possible the source of ATP in the cochlea,contained ATP that maybe existed in a non-vesicular form or specific staining of non-SYN.

OBJECTIVE To study the drug resistance of Stenotrophomonas maltophilia in order to direct clinical drug application.METHODS The S.maltophilia was identified and its sensitivity was analyzed with VITEK2-AMS.The extended-spectrum beta-lactamases(ESBLs) and metallo ?-lactamase(MBL) were screened by double-disk synergy test and double-disk confirmatory test.RESULTS The resistance rate of S.maltophilia to cefoperazone-sulbactam,levofloxacin and SMZ-TMP was the lowest,accounted for 12.50%,13.64% and 14.77%,respectively.The ESBLs positive rate was 69.32%,the MBL positive rate was 69.32%.CONCLUSIONS The multidrug resistance of S.maltophilia is serious and full dose use of two or more antibacterials is recommended.

Accidents, Occupational ; Acrylamide ; poisoning ; Adolescent ; Critical Illness ; Humans ; Male ; Young Adult

Adult ; Aged ; Benzidines ; toxicity ; Coloring Agents ; Humans ; Male ; Middle Aged ; Occupational Exposure ; Urinary Bladder Neoplasms ; epidemiology

This article was a perspective study on natural pigment's new microbial resources,culture conditions,fermentation processes and genetic engineering strains,which provides some future direction for the development and application of natural pigment.

ObjectiveTo study predictive value of dysphagia manifestations in clinical physical and vediofluoroscopy exam for aspiration, silent aspiration, pneumonia and nutritional status. MethodsAll kinds of manifestations in clinical and videofluoroscopy were analyzed with chi square test according to number of patients with aspiration, silent aspiration, pneumonia and grades of nutritional status at discharge. Those manifestations with statistic significance were analyzed by Logistic regression to test their quantitive predictive value. ResultsClinical characteristics can predict some manifestations under videofluoroscopy and the manifestations which can predict aspiration, silent aspiration, pneumonia in hospital and nutrition status at discharge were attained.ConclusionThese clinical and imagic predictive manifestations to aspiration, pneumonia and nutrition status are attained, which may be useful to clinical practice.

Objective To investigate the relationship between the ankle-brachial index (ABI)and the extent of coronary artery stenosis and the left ventricular function in elderly patients with coronary heart disease (CHD)with metabolism syndrome (MS). Methods A total of 140 patients with coronary artery disease was study subjects. In which, 66 cases complicated MS (MS group), 74 cases without MS (non MS group). ABI and the left ventricular function was examined in an patients. Results ABI was 0.90±0.32 in MS group, and 1.03±0.26 in non MS group, there was significant difference between two groups(P=0.015). Compared with non MS group, significant lower ratio of ABI in MS group [43.9%(29/66) vs 27.0%(20/74)] (P=0.038); whereas much more 3-vessel disease in ABI≤0.9 patients compared with those of ABI > 0.9 patients [86.3%(25/29) vs 37.8%(14/37) ], significant lower of the left ventricular function in ABI≤0.9 patients compared with those of ABI >0.9 patients (P<0.05). Conclusion There is more severe of coronary artery stenosis in ABI≤0.9 patients for CHD with MS, more attention should be payed to CHD patients combined with MS.

Humans ; Musculoskeletal Diseases ; psychology ; Occupational Diseases ; psychology ; Psychology, Social

Objective To explore the effects of mechanical ventilation on the treatment of acute severe left heart failure.Methods While the routine drug therapy was used,25 patients with severe acute left heart failure received mechanical ventilation treatment.The changes of their artery blood gas,vital signs and clinical symptom were observed before and after mechanical ventilation.Results The parameters of their blood gas and clinical symptom were dramatically improved after the mechanical ventilation therapy in 21 patients(P＜0.01).19 patients were saved successfully and 6 patients died.Conclusion The mechanical ventilation could effectively improve the clinical symptoms and respiratory function of patients with acute severe left heart failure,which is an effective method in the adjuvant treatment of patients with acute severe left heart failure.

WHO has issued three editions of pathologic classification of bladder urothelial carcinoma in 1973,1999 and 2004.The 1973 version classification had been widely and the longest applied.However,WHO 2004 classification had been prevalent in past years.There were two issues in the applications of WHO 2004 classification.On one hand,there were some difficulties in quick grading in a given case.On the other hand,there were some misunderstandings in the conversion of different WHO classification.In this article,the changes of different pathologic classification of bladder urothelial carcinoma were reviewed and the outline of different pathologic classification was generalized.The criterion of all the systems was cell anaplasia.In WHO 1973 version classification,the definition of the various grades was vague.It was relatively precise in WHO 1999 classification.However,the grading of Ⅰ,Ⅱ and Ⅲ in WHO 1999 classification still remained confusions.The major changes in WHO 2004 classification was that this system divided urothelial carcinoma into low-and high-grade,which may solve the heterogenesis of grade Ⅱ in the other two classifications.