Objective To investigate the expression of Rac 1 in the guinea pig cochlea of gentamicin-induced ototoxic damage and prevention with antioxidant sodium salicylate , and to explore the roles of Rac 1 in the ototoxic mechanism of aminoglycoside antibi-otics.Methods Thirty healthy male guinea pigs were involved in this study .All the guinea pigs were randomly divided into 3 groups and received intraperitoneal injections according to their arranged group .Group I (control) was treated with normal saline for 7 days. Group II [Gentamicin (GM)] was treated with gentamicin alone for 7 days.Group III [GM+sodium salicylate(SA)] was treated with gentamicin in combination with sodium salicylate for 7 days.Paraffin-embedded cochlear section with immunohistochemical stai-ning was used for evaluation of Rac 1 expression in the cochlea .The protein was extracted from the cochlea tissues , and Rac1 protein levels in the cochlea were detected by Western blot assay .Results Immunohistochemistry showed a slightly positive reaction for Rac 1 staining found in the cochlea of the control group was mainly shown in the cytoplasm and cytomembrane of ganglion and organ of Corti ;a highly positive expression was shown in GM group;and the extent of Rac 1 expression of GM+SA group was between the control and GM groups.Image analysis showed that the differences in Rac1 expression between each two groups were statistically significant ( P <0.05).Western blot assay showed that expression of Rac 1 protein was highest in GM group and decreased in GM +SA group, while the intensity of Rac1 protein expression in GM +SA group was between the control and GM group .Statistics analysis showed that the expression between each two groups had significant difference ( P <0.05 ) .Conclusions A slightly positive reaction for Rac 1 stai-ning was found in the cochlea of guinea pigs , and mainly observed in the cytoplasm and cytomembrane of spiral ganglion and organ of Corti.With the administration of gentamicin , Rac1 protein expression was upregulated in the cochlea .Simultaneous administration of antioxidant sodium salicylate could significantly decrease the expression of Rac 1 protein.These results indicated that Rac1 might play an important role in the processes of gentamicin-induced oxidative damage of cochlea .

Objective To investigate the changes of plasma insulin and C peptide level in women with gestational diabetes mellitus(GDM), gestational impaired glucose tolerance(GIGT) and normal pregnant and to find out the patterns of glucose metabolism in pregnancy and the function of islet ? cell in patients with gestational impaired glucose metabolism Methods Thrity one normal pregnant women (group Ⅰ), 36 GIGT (group Ⅱ)and 31 GDM (group Ⅲ) were selected. Insulin and C peptide levels were measured by radioimmunoassay. Results Insulin and C peptide levels of groups Ⅰand Ⅲ increased with the gestational age and decreased at postpartum. At 31 weeks of gestation, insulin and C peptide levels of group Ⅱ and group Ⅲ were significantly higher than that of group I [insulin: (13.95?3.15) mIU/L and (15.10?2.96)mIU/L vs (11.40?3.27) mIU/L; C peptide: (0.60 ?0.21) pmol/ml and (0.80?0.31) pmol/ml vs (0.44?0.26) pmol/ml, P 0.05). The peak value of insulin and C peptide was found at the first hour postprandially in group I, while at the second hour in group Ⅱ and Ⅲ. Conclusions Insulin resistance during pregnancy becomes stronger gradually with pregnant progress. Women with abnormal glucose metabolism during pregnancy would have more insulin resistance which leads to the damage of insulin secretion. These women should have long term followed up.

BACKGROUND:Klinefelter nails and external fixation were commonly used treatment methods for Bennett fracture, but study about biomechanics of two methods was little. OBJECTIVE:To establish three-dimensional finite element models of Bennett fracture by Klinefelter nails and external fixation, and analyze its biomechanics. METHODS:Three-dimensional models of Bennett fracture were established by smal external fixation and Kirschner nail treatment. The Bennett fracture models established by smal external fixation treatment were considered as model A. Bennett fracture models established by Kirschner nail treatment were considered as model B. 120 N load was applied to the first metacarpal X-axis direction. The X-direction displacement and total displacement of model A and model B, and the fracture fragments stress of model A and model B were observed. RESULTS AND CONCLUSION:(1) In the X direction, the absolute values of maximum displacement and minimum displacement of model A at proximal fragment and distal fragment were less than model B;the maximum relative displacement, minimum relative displacement and average relative displacement of model A were less than model B. The differences of the average displacement of the proximal fracture fragment and the distal fragment, and the relative mean displacement in two models were statistical y significant (P<0.05). (2) In total displacement, absolute values of the maximum displacement and minimum displacement of model A at proximal fragment were less than model B;absolute values of the maximum displacement and minimum displacement of model A at distal fragment were less than model B;the maximum relative displacement, minimum relative displacement and average relative displacement of model A were less than model B. The differences of the average displacement of the proximal fracture fragment and the distal fragment, and the relative mean displacement in two models were statistical y significant (P<0.05). (3) The maximum stress value, a minimum stress value and the average stress values of fracture fragments of model A were significantly smal er than model B. The difference of the mean stress value in two models was statistical y significant (P<0.05). (4) These findings showed that the X-direction displacement and the total displacement were smal er, and the fracture fragments stress was uniform in smal external fixation and Kirschner nail treatment for Bennett fracture, indicating that smal external fixation for Bennett fracture has more advantages compared with Klinefelter nail.

0. 05). but there was a significant difference between the treatment group and the Chinese patent medicine control group (P

Child ; Humans ; Practice Guidelines as Topic ; Syncope ; diagnosis

Animals ; Antineoplastic Agents ; toxicity ; Cisplatin ; toxicity ; Drugs, Chinese Herbal ; pharmacology ; Hearing Loss ; chemically induced ; Male ; Mice ; Mice, Inbred Strains ; Salvia miltiorrhiza

China ; Health Surveys ; Humans ; Physical Fitness ; Students

OBJECTIVETo evaluate the clinical outcomes and complications of Triple-Endobutton plates in treating Tossy type III acromioclavicular joint dislocation.

METHODSFrom January 2011 to January 2013,45 patients with Tossy type III acromioclavicular joint dislocation were treated with Triple-Endobutton plates. There were 35 males and 10 females with an average age of 30.5 (ranged from 19 to 60) years old. At the final follow-up, VAS, DASH, Constant-Murley criterion were used to evaluate shoulder function.

RESULTSAll patients were followed up from 15 to 36 months. No neurovascular injury, wound infection and stress fractures were found,but 3 patients had a re-dislocation. At the final follow-up,the mean VAS score was decreased from (5.7±1.6) preoperatively to postoperative (0.2±0.1); DASH score was significantly decreased from (19.6±4.3) preoperatively to (0.3±0.1) postoperatively; Constant-Murley score was improved from (34.4±4.3) before operation to (94.8± 3.5) after operation.

CONCLUSIONClinical outcomes of treating Tossy type III acromioclavicular joint dislocation with Triple-Endobutton plates is satisfactory. However, re-dislocation is still the most common complication. Careful perioperative management is an important factor in preventing re-dislocation.

Acromioclavicular Joint ; injuries ; surgery ; Adult ; Bone Plates ; Female ; Follow-Up Studies ; Fracture Fixation, Internal ; instrumentation ; methods ; Humans ; Male ; Middle Aged ; Shoulder Dislocation ; surgery ; Treatment Outcome ; Young Adult

Objective To investigate the value of lumbar puncture in the diagnosis of subarachnoid hemor-rhage of aneurysms .Methods The clinical data of 13 patients diagnosed negative by computer tomography(CT) scanning and then as subarachnoid hemorrhage (SAH) followed by lumbar puncture and as aneurysms by catheter digital subtraction angiography(DSA) were retrospectively analyzed.Results The positive rate was 100% by lum-bar puncture.Conclusion Lumbar puncture is of great significance in the diagnosis of subarachnoid hemorrhage of aneurysms, which should be applied reasonably.

Objective To investigate the therapeutic effect of acupuncture combined with umbilical ring point ginger-partition moxibustion for ulcerative colitis of spleen-kidney yang deficiency type, and to observe its influence on the related inflammatory factors. Methods Sixty qualified cases were randomly divided into treatment group and control group, 30 in each group. Both groups were given oral use of live combined bifidobacterium, lactobacillus and enterococcus capsules, and the treatment group received acupuncture and umbilical ring point ginger-partition moxibustion together. After one treatment course ( four weeks) , the therapeutic effect was evaluated, and the changes of serum levels of related inflammatory factors of tumor necrosis alpha (TNF-α) and interleukin 8 (IL-8) were observed. Moreover, the safety was monitored. Results(1) The total effective rate was 86.67% in the treatment group and 66.67% in the control group, the difference being significant between the two groups (P<0.05). (2) The serum TNF-α and IL-8 levels were decreased in both groups after treatment (P<0.05 or P<0.01), and the decrease in the treatment group was superior to that in the control group ( P<0.05). ( 3) No obvious reverse action was found in the two groups. Conclusion Acupuncture combined with umbilical ring point ginger-partition moxibustion is effective and safe for the treatment of ulcerative colitis of spleen-kidney yang deficiency type.