OBJECTIVE:To establish an HPLC method for simultaneous determination of Naringin,Hesperidin and Neohesperidin in the effective fraction of flavone in Fructus Aurantii Immaturus.METHODS:Hypersil C18 column(250 mm?4.6 mm,5 ?m) was used with the mobile phase consisted of acetonitrile -0.1% phosphoric acid solution(20∶80) at a flow rate of 1.0 mL?min-1,and the detection wavelength was set at 283 nm and the column temperature was maintained at 30 ℃.RESULTS:The calibration curves of Naringin,Hesperidin and Neohesperidin were in good linearity over the ranges of 0.44～2.20 ?g(r=0.999 5),0.025 6～0.128 0 ?g(r=0.999 3),0.54 ～ 2.70 ?g(r=0.999 9),respectively.And the average recoveries for the three constituents were 99.41%,100.33% and 99.69%,respectively with RSD at 1.14%,1.47%,and 1.16%,respectively.CONCLUSION:The method is simple,accurate and reliable,and can be used for the quality control of Fructus Aurantii Immaturus.

BACKGROUND: To enhance peripheral nerve regeneration, the focus on the development of nerve conduits has been shifted from conventional conduits without internal structure to novel conduits with internal stuucture.Therefore, a comprehensive understanding of the internal structure of nerve conduits is necessary.OBJECTIVE: To review the application of tissue-engineered nerve conduits with internal structure in nerve regeneration.METHODS: A computer-based research of CNKI, WanFang, SinoMed and PubMed databases was performed to retrieve relevant literatures about the tissue-engineered nerve conduits with internal structure published from January 2009 to December 2015, using the keywords of nerve conduit/conduits/scaffolds/scaffold/channel/channels, internal scaffolds/scaffold, nerve regeneration/repair, hydrogel, acellularized/decellularized nerve in Chinese and English,respectively. Forty-seven eligible literatures were included for overview.RESULTS AND CONCLUSION: The novel nerve conduits with internal structure have many advantages over conventional conduits without internal structure, which can support, guide, promote peripheral nerve regeneration and functional recovery, especially, when modified with seed cells, neurotrophic factors or extracellular matrix, can achieve more effective results comparable to autologous nerve grafts.

AIM:To explore the effect of comprehensive therapy in the treatment of fungal corneal ulcer and clinical outcome.
METHODS:A total of 159 cases 159 eyes fungal corneal ulcer patients took comprehensive therapy of 10 measures, followed up more than 3mo to observe the treatment effect and the final visual acuity in our hospital for treatment.
RESULTS: The average hospitalization time of fungal corneal ulcer was(12. 11±5. 13) d. A total of 148 cases of 148 eyes were cured, and the cure rate was 93. 1%;improvement in 8 cases, accounted for 5. 0%; 3 cases were ineffective, accounted for 1. 9%. The final visual acuity of 89 eyes ≥0. 3, accounted for 56. 0%(89/159). The final visual acuity of 136 eyes ≥0. 1, accounting for 85. 5%(136/159), and accounting for 91. 9% in 148 cured patients.
CONCLUSION: The comprehensive therapy in the treatment of fungal corneal ulcer has higher cure rate and visual acuity.

The eight scalp needles, founded by Professor QIN Liang-fu, and its clinical experience for treatment of Parkinson's disease (PD) are introduced. Based on his years of clinical experience, it is proposed by Professor QIN that the Governor Vessel is mainly for miscellaneous disease and disease of limbs. Combined with distribution of cephalic motor region and meridian, an acupuncture treatment plan that is full of innovativeness is proposed, which is called Qin's eight scalp needles. It includes bilateral Fengchi (GB 20), Shuaigu (GB 8), Toulinqi (GB 15) as well as Yintang (GV 29) and Baihui (GV 20), mainly for treatment of nervous system diseases, such as PD and multiple sclerosis and so on. Besides, some outpatient cases are introduced to explain that eight scalp needle could alleviate the progression of PD, improve patients' motor, cognitive and affective disorders, reduce the suffering of patients, and improve the patient's quality of life.
Acupuncture Points ; Acupuncture Therapy ; history ; methods ; China ; Female ; History, 20th Century ; History, 21st Century ; Humans ; Male ; Middle Aged ; Parkinson Disease ; history ; therapy ; Scalp

Arteriosclerosis Obliterans ; surgery ; Humans ; Lower Extremity

Laparoscopic partial nephrectomy is gaining more and more popularity in treating patients with early renal carcinoma in recent years. Compared with radical nephrectomy, laparoscopic partial nephrectomy is a mini-invasive, safe method with satisfactory postoperative survival rate. However, there were still limited laboratory and clinical data about laparoscopic partial nephrectomy, and the experimental data of traditional laparoscopic surgery and open surgery were still used. Based on the existing data and the authors' experience, this article proposes three hypotheses for the problems puzzling urologic surgeons; the authors hope to verify the hypotheses through basic or clinical research.

Objective: To explore the procedures and clinical outcomes of retroperitoneal partial nephrectomy for early malignant renal tumors. Methods: A total of 21 patients (19 men and 2 women, with a mean age of 49 years) with early malignant renal tumors were subjected to retroperitoneal partial nephrectomy using ultrasound knife and electron microscopy. The 21 patients included 17 with clear cell renal carcinoma, 3 with granule cell renal carcinoma, and 1 with oxyphil cell renal carcinoma. The mean diameter of the tumors was (2.8±0.8) cm. Results: All the 21 cases underwent successful retroperitoneal partial nephrectomy without conversion to open operation. Mean surgical time was (105 ± 15) min and mean blood loss was (120 ± 22) ml. Four patients had a mean blood transfusion of 400 ml. One patient had urine leakage, with drainage volume of 200-300 ml; the drainage was stopped 15 days after operation when the drainage was less than 20 ml. The mean postoperative hospital stay was (9±2) days (7-17 days). No patients had local recurrence during a mean follow up of (20±4) months. Conclusion: Laparoscopic partial nephrectomy is feasible and safe.

Objective: To summarize our experience on laparoscopic adrenalectomy. Methods: From August 2001 to Jun 2007, a total of 203 patients (128 male and 75 female, aged 21-74 years, with a mean of [45±2] years old) received laparoscopic adrenalectomy. Six patients had bilateral tumors. The diameters of the adrenal tumors or nodules were 0.4-18 cm. Results: The 203 patients received a total of 209 times of laparoscopic adrenalectomy: including 34 cases via transperitoneal approach, 166 via retroperitoneal approach and 3 via hand-assisted approach. The overall successful rate of laparoscopic adrenalectomy was 97.04% (197/203). Six person-times (2.96%) were converted to open operation due to bleeding or adhesion. The mean operation time was 135±35 min and the estimated blood loss was 40-250 ml (with a mean of 75±25 ml). The patients could get down the bed for activity 1-3 days after operation. The postoperative hospital stay was 7-10 days (with a mean of 7± 2 days). The complications included vena caval injury (1 case), pleural injury (1 case), lumbar hematoma (4 cases), and fat liquefaction (1 case). Conclusion: Laparoscopic adrenalectomy has advantages for treating adrenal tumors. But different approaches should be chosen according to the size and pathological types of the tumors. Transperitoneal approach should be chosen for patients with larger tumors, for obese patients or for patients with bilateral lesions.

Objective: To discuss the procedure and clinical effect of retroperitoneal laparoscopic nephropexy (RLN). Methods: From August 2001 to June 2006, RLN was performed on 28 female patients aged 26-45 years old (mean, 34±2.5) with symptomatic nephroptosis, including 15 with the right kidney, 12 with the left, and 1 with both. The preoperative complaint of patients included subjective symptoms (constant and recurring pain in 28 patients) and objective symptoms (upper urinary infections in 16, hematuria in 12, and upper tract obstruction in 12). One patient underwent nephropexy via the transperitoneal approach and the others underwent nephropexy via the retroperitoneal approach. A retroperitoneoscopic procedure was performed after positioning the patients in the flank position. Digital preparation of the retroperitoneal space was made and standardized trocar was placed. The key step of the surgery was complete exposure of the kidney within Gerota' fascia, which was aimed to separate the potential adhesions between the colon and kidney or between the inferior blood vessels of the kidney. Nephropexy was performed between the fibrous capsule at the lower pole of the kidney and the dissected psoas muscle, using three sutures placed by intracorporeal technique or the percutaneous needle both for introduction and removal of the suture; the sutures were separately tied over the sacrospinalis fascia. Results: The mean operative time was (125±9) min (ranging 115-240 min); the mean postoperative hospital stay was (9±1.2) days, largely owing to the required 5-12 days' bed rest. During a mean follow-up of (24±4.2) months(ranging 3 to 70 months), 3 patients had paresthesia, 5 had constant and recurrent ache, 20 were completely free of pain, and 4 had micro-hematuria. One patient had further episodes of pyelonephritis and upper tract obstruction after operation. Intravenous pyelogram(IVP) revealed that the ptosis incorporated into more than one vertebral body in 2 patients. Postoperative renal function test showed an improvement in renal function. Conclusion: RLN is mini-invasive and has less complication. The procedure should be considered as one of the optimal therapy for nephroptosis.