Objective:To investigate whether interfering survivin has the potential to sensitize stomach cancer cells to Epirubicin.Methods:By the method of liposome transfection,survivin antisense oligodeoxynucleotide was transfected into human stomach cancer SGC-7901 cell line.Treat cells with different concentrations of survivin ASODN and Epirubicin respectively to select the best combination trough assessing the growth of cells by MTT assay.Cells were randomly divided into control group,ASODN group,Epirubicin and combination group.Western blot analysis was used to check the survivin protein expression;inhibitive rate were detected by MTT assay;Flow cytometric analysis was used to detect the cycle status of SGC-7901 cells.The apoptosis was screened according to the result of Annexin V-FITC.Reverse transcription polymerase chain reaction(RT-PCR)were applied to detect the expression of bcl-2、SMac mRNA.Results:MTT assay showed the proliferation of SGC-7901 cells was retarded obviously contrasting to control group.The level of survivin protein expression in ASODN group are lower than control group according to Western blot analysis.Annexin V-FITC show that the apoptosis rate of ASODN group cells increases.The level of SMac mRNA expression in ASODN group are higher than control group according to RT-PCR analysis.Conclusion:Survivin siRNA can sensitize stomach cancer cells to Epirubicin.Up-regulating the SMac expression might be one of mechanisms.

Objective To prepare the glycyrrhizic acid solid lipid nanoparticles(GL-SLN).Methods GL-SLN was prepared by conventional rotary-evaporated film-ultrasonication method.Based on the single-factor experiment,GL-SLN was prepared using orthogonal design for optimization of formulation and technology.The obtained GL-SLN was also evaluated.Results GL-SLN had even and regular appearance and the average diameter was 75.8 nm.Zeta potential was-19.7 mV.The drug loading was 8.27% and the entrapment ratio was 91.76%.Conclusion The entrapment ratio of GL-SLN and the stability of GL-SLN prepared by conventional rotary-evaporated film-ultrasonication method are high and good.

OBJECTIVE: To prepare Berberine hydrochloride solid lipid nanoparticles(BH-SLN). METHODS: BH-SL-N was prepared by conventional rotary-evaporated film-ultrasonication method. Based on the single-factor experiment, BH-SLN was prepared using orthogonal design for optimization of formulation and technology. The quality of the obtained BH-SLN was evaluated. RESULTS:BH-SLN had even and regular appearance with a mean diameter of 60.5 nm,Zeta potential of —29.7 mV,drug loading of 8.69%, and entrapment ratio of 97.58%. CONCLUSION: The entrapment ratio of BH-SLN is high and the stability is good for BH-SLN prepared by conventional rotary-evaporated film-ultrasonication method, and the method is reliable.

Objective:To investigate the effects of human factor Ⅸ (FⅨ) intron 1 inserted in forward orientation into retroviral vector on the expression of FⅨ in muscle cells. Methods: Two FⅨ mini-genes, FⅨm1 and FⅨm2, were inserted in forward orientation into retroviral vector backbone, LⅨSN, constructing 2 vectors named LⅨm1SN and LⅨm2SN, containing shortened intron of 1.4 and 0.3 kb, respectively. Retrovirus was made by transfecting PA317 packaging cells. Transient and stable expression of FⅨ was tested in SCID mouse muscle cells (SCID-MB). Results: The vector titers and expression levels of LⅨm1SN and LⅨm2SN in SCID-MB were 1 to 2 folds higher than that of LⅨSN. Some SCID-MB colonies transfected with LⅨm2SN contained intact FⅨm2 inton elements, suggesting that splicing of virus mRNA may not be complete in packaging cells. Conclusion: FⅨ intron 1 inserted in forward orientation in retroviral vectors can increase retroviral titer and the expression level of FⅨ in muscle cells.

In autoimmune thrombocytopenic purpura (AITP) specific autoantibodies bind platelet GP via their Fab fragments. Both splenic CD5+ B and CD5- B cells produce platelet glycoprotein-specific antibodies. There is limited number of antigenic determinants, and the GP-specific autoantibodies are derived from a restricted number of B-cell clones in chronic AITP. Blocking co-stimulatory signals could induce platelet-specific T cell anergy. MMF could be used as a second line agent for the treatment of steroid-resistant AITP. Detection of plasma thrombopoietin levels play an important role in the differentiation of thrombocytopenic states caused by platelet destruction or due to bone marrow hypoplasia. Endogenous TPO level is also important on the differential diagnosis of ET and RT. Quinine- or heparin-dependent antibodies could induce thrombocytopenia. PCR-SSP is useful for the genotyping of the platelet-specific alloantigen HPA. Biotinylated platelets have an impaired response to agonists as evidenced by in vitro platelet aggregation tests.
Antigens, Human Platelet ; immunology ; Blood Platelets ; immunology ; Heparin ; adverse effects ; Humans ; Platelet Membrane Glycoproteins ; immunology ; Purpura, Thrombocytopenic, Idiopathic ; etiology ; immunology ; Thrombopoietin ; blood

Adult ; Humans ; Purpura, Thrombocytopenic, Idiopathic ; diagnosis ; therapy

Objective: To design qualitative quality control testing device for portable medical endoscope optical performance. Methods:To install the mounting bracket, uniform light source and testing target, etc and integrate testing of the visual resolution, geometric distortion, color reproduction capability, the dynamic range and the brightness of the image plane on only one testing target. Results:This kind of device can observe the target by eye or monitor. Conclusion:It can realize the function of rapid, qualitative detection for clinical medical endoscope optical performance parameters.

Objective To compare the efficacy of arthroscopic synovectomy between early and middle stage rheumatoid arthritis. Methods A total of 34 cases of rheumatoid arthritis (42 knee joints) either in early stage (22 cases, 24 joints) or in meddle stage (12 cases, 18 joints) underwent arthroscopic synovectomy. The bipolar radiofrequency was used for the synovial debridement and hemostasis in 32 knee joints. Postoperatively, routine anti-rheumatoid drugs were administrated. Follow-up observations in the two groups included the evaluation of knee function, the erythrocyte sedimentation rate (ESR), the C-reaction protein (CRP), and the rheumatoid factor (RF). Results The rate of excellent or good results was 91.7% in early stage (22/24) and 66.7% in middle stage (12/18), without statistical significance between the two groups ( ? 2=2.705, P =0.100). Conclusions Arthroscopic synovectomy is effective for the treatment of rheumatoid arthritis in both early and middle stage. Bipolar radiofrequency is helpful for the complete elimination of the synovium, the prevention of joint hematoma, and the rehabilitation of joint function.

Objective To study the ultrastrueture of 3 types of periodontal cells, including periodontal ligament cells (PDLC), os-teoblast cells (OB) and eementoblast cells (CB). Methods After culturing 3 kinds of periodontal cells - PDLC, OB and CB, we observed them with transmission electron microscope. Results There was rich rough endoplasmic reticulum and lots of microfilaments in the cyto-plasm of CB and PDLC cells. There was rich rough endoplasmic in the cytoplasm of OB cells. Conclusion The main characteristic ultra-structure feature of the bovine CB and PDLC was rich rough endoplasmic reticulum and microfilaments in the cytoplasm. Compared with CB and PDLC, OB contained fewer microfilaments in the cytoplasm.

Objective To introduce and analyze the design, manufacture and clinical effect of the casting full dentition splint in treatment of periodontal disease with or without dentition defect. Methods 148 patients with periodontal disease during 1995 to 1998 were treated with casting full dentition splint after a thorough basic periodontal treatment. The patients were composed of 81 men and 67 women aged 40-69y. The patients were followed up for 1 to 3 years after restoration. General effects of the prosthesis were evaluated based on the chief complain of patients, clinical examination and X-ray examination. Results Among 148 patients, 140 (94.59%) were satisfied with the prosthesis and stable periodontal status, 6 (4.05%) were improved, 2 (1.35%) showed no clinical effect. Conclusion Casting full dentition splint can improve the mastication efficiency in periodontal disease patients. It is an effective and reversible therapeutic method on treatment of the periodontal disease with or without dentition defect.