It has been shown that the time constant of heart rate decline for the first 30 sec (T₃₀) after exercise, at an intensity lower than the ventilatory threshold (VT), can serve as a specific index to assess post-exercise vagal reactivation. The purpose of this study was to validate the use of a simpler alternative index, i. e. %Δ HR₃₀ (the ratio of heart rate decrement for the first 30 sec after exercise) for the evaluation of parasympathetic nervous reactivation, and to examine whether it would be a useful index in the conditioning of athletes. Eighteen college students performed 4 minutes cycle ergometer exercise routines at intensities of 40%, 80%, and 120% VT to compare the %Δ HR₃₀ and the T₃₀. In addition, the %Δ HR₃₀ was obtained by a field test (4 minutes jogging) in 15 college middle and long distance runners, every morning during summer camp training to assess the state of athletic conditioning. The %Δ HR₃₀ at 80% VT was similar to the value at 40% VT, but significantly different from the value at 120% VT, as was the T₃₀ at 80% VT.
The %Δ HR₃₀ significantly correlated with the T₃₀ and VO₂max. During the camp, the %Δ HR₃₀ was higher on mornings following light training days than on mornings following hard training days. These results suggest that the %Δ HR₃₀, at an exercise intensity lower than the VT, could be a simple and useful index to evaluate post-exercise parasympathetic nervous reactivation in the conditioning of athletes.

A double blind randomized control trial was performed to clarify the effects of acupuncture using a press needle on muscle pain, serum creatine kinase (CK) activity and muscle stiffness that resulted from running a marathon race. Sham press needles (placebo needles), which have the same package but without a needle tip, were developed by the acupuncture study authors. The subjects were 15 university students who participated in a marathon for the first time. Subjects were randomly assigned to two groups: the real acupuncture group and the placebo acupuncture group.
Both the real and the sham needles were applied to eight traditional Chinese acupoints in the lower limbs. They were applied before the start of the race and removed five days after the race.
Physical and biochemical examinations to determine the degree of muscle pain, CK activity, LDH isozyme, body flexion in standing position and muscle hardness were evaluated three times-before the start, after the finish, and five days after the finish.
Result: 1) The real acupuncture group showed less muscle pain than the placebo acupuncture group. 2) CK activity and LDH4-5 showed higher levels after the finish than before the start, but no significant difference was obtained among the groups. 3) No significant difference in time course change of the body flexion was obtained among the groups. 4) Hardness of vastus lateralis and vastus medialis showed higher levels after the finish than before the start, but no significant difference was obtained among the groups.

BACKGROUND: Pyrethroid (PYR) insecticides are widely used for controlling various pests. There are two types that differ in terms of usage: agricultural-purpose PYR (agriculture-PYR) and hygiene purpose PYR (hygiene-PYRs). Few studies exist on the exposure to these chemicals in small children. In this study, we conducted biomonitoring of urinary pyrethroid metabolites in 1.5-year-old children throughout the year. METHODS: Study subjects were 1075 children participating in an Aichi regional sub-cohort of the Japan Environment and Children's Study as of 18-month health check-up. The concentrations of four specific hygiene-PYR metabolites including 2,3,5,6-tetrafluoro-1,4-benzenedimethanol (HOCH₂-FB-Al), and five common metabolites of hygiene- and agriculture-PYRs including 3-phenoxybenzoic acid (3PBA) and cis- and trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DCCA), were measured in urine samples extracted from soiled diapers using a triple quadrupole gas chromatograph-mass spectrometer. RESULTS: The highest detection frequencies were for 3PBA, followed by DCCA, 1R-trans-chrysanthemum dicarboxylic acid, and HOCH₂-FB-Al. Among the six metabolites, urinary concentrations were seasonally varied. However, this variation was not observed in the most studied PYR metabolite, 3PBA. Spearman's correlation analysis demonstrated a significant positive correlation between FB-Al and DCCA (r = 0.56) and HOCH₂-FB-Al and 4-methoxymethyl-2,3,5,6-tetrafluorobenzyl alcohol (r = 0.60). CONCLUSIONS This biomonitoring survey found widespread and seasonally specific exposure to multiple hygiene- and agriculture-PYRs in 1.5-year-old Japanese children.
Agriculture ; Child, Preschool ; Environmental Exposure/analysis* ; Humans ; Infant ; Insecticides ; Japan ; Mass Spectrometry ; Pyrethrins/urine*

BACKGROUND: Glyphosate and its salt formulations are nonselective herbicides that have been extensively used worldwide, both for residential and agricultural purposes. The possible carcinogenicity and teratogenicity of glyphosate remain to be elucidated. We developed a sensitive and high-throughput analytical method for urinary glyphosate using liquid chromatography-tandem mass spectrometry with the aim of contributing to glyphosate exposure assessment in epidemiological studies. METHODS: After urine dilution (creatinine matching dilution to 0.05 g creatinine/L), glyphosate was extracted using two types of solid phase extraction columns (SCX and NH2) with automated sample preparation instruments. The eluate was dried and dissolved in the mobile phase, followed by liquid chromatography-tandem mass spectrometry analysis. The optimized method was applied to urine samples obtained from 54 Japanese adults and children. RESULTS: The results from the validation study demonstrated good recoveries (91.0-99.6%), within- and between-run precisions (< 15%), low detection limits (0.1 μg/L), and lower limit of quantification (0.3 μg/L). The detection frequency and median concentration of the urinary glyphosate in Japanese subjects were 59% and 0.25 μg/L (0.34 μg/g creatinine). CONCLUSIONS Our reliable determination method was successful in measuring urinary glyphosate concentration. Moreover, this is the first biomonitoring report of urinary glyphosate levels in the Japanese general population.
Adult ; Aged ; Chromatography, Liquid/methods* ; Female ; Glycine/urine* ; Humans ; Male ; Middle Aged ; Solid Phase Extraction/methods* ; Tandem Mass Spectrometry/methods*

BACKGROUND: The Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) study was launched to investigate risk factors for osteoporotic fractures, interactions of osteoporosis with other non-communicable chronic diseases, and effects of fracture on QOL and mortality. METHODS: FORMEN baseline study participants (in 2007 and 2008) included 2012 community-dwelling men (aged 65-93 years) in Nara prefecture, Japan. Clinical follow-up surveys were conducted 5 and 10 years after the baseline survey, and 1539 and 906 men completed them, respectively. Supplemental mail, telephone, and visit surveys were conducted with non-participants to obtain outcome information. Survival and fracture outcomes were determined for 2006 men, with 566 deaths identified and 1233 men remaining in the cohort at 10-year follow-up. COMMENTS The baseline survey covered a wide range of bone health-related indices including bone mineral density, trabecular microarchitecture assessment, vertebral imaging for detecting vertebral fractures, and biochemical markers of bone turnover, as well as comprehensive geriatric assessment items. Follow-up surveys were conducted to obtain outcomes including osteoporotic fracture, cardiovascular diseases, initiation of long-term care, and mortality. A complete list of publications relating to the FORMEN study can be found at https://www.med.kindai.ac.jp/pubheal/FORMEN/Publications.html .
Aged ; Bone Density ; Cardiovascular Diseases/etiology* ; Cohort Studies ; Geriatric Assessment ; Humans ; Independent Living ; Japan/epidemiology* ; Long-Term Care/statistics & numerical data* ; Male ; Middle Aged ; Osteoporosis/etiology* ; Osteoporotic Fractures/etiology* ; Risk Factors