Child, Preschool ; Communicable Disease Control ; Diarrhea ; prevention & control ; virology ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Virus Diseases ; prevention & control ; virology ; Virus Physiological Phenomena ; Viruses ; isolation & purification

China ; Diarrhea ; prevention & control ; virology ; Humans ; Viral Vaccines ; immunology ; Virus Diseases ; prevention & control

Acute Disease ; Child ; Humans ; Respiratory Tract Infections ; virology

Administration, Oral ; Humans ; Rotavirus Infections ; immunology ; prevention & control ; Viral Vaccines ; administration & dosage ; immunology

Actinomycosis ; pathology ; Adult ; Diagnosis, Differential ; Female ; Humans ; Mycoses ; pathology ; Staphylococcal Skin Infections ; microbiology ; pathology ; surgery ; Staphylococcus aureus ; isolation & purification ; Young Adult

Objective:To study the characters of perioral muscle p re ssure of the adult patients with skeletal crossbite. Methods:20 patients were diagnosed to be with skeletal crossbite. The perioral muscle press ures on upper and lower central incisors, first molars and cuspids were measured buccally and lingually at rest position. 10 health adults were served as the co ntrols. Results:The perioral force in skeletal crossbite group was larger than that in the control group on the correspondence area(P0.05). In the skeletal cross bite group, the perioral force on the mandibular area was larger than that on th e correspondence maxillary area(P0.05). The muscle pressure on the labial side was larger than that on t he lingual correspondence side in both groups(P

Objective: To construct a recombinant human inhibitor of growth 4 (hING4) gene and observe its growth inhibition effect on lung adenocarcinoma NCI H460 cells. Methods: The GFP-labeled recombinant adenovirus vector hING4 (Ad-hING4) was constructed using pcDNA3. 0-mING4 plasmid as a template and site-specific mutagenesis technique. RT-PCR and fluorescence microscope were used to detect the expression of hING4 in NCI H460 cells. Bax and Bcl-2 protein expression were detected by Western blotting. Colony formation assay and flow cytometry were used to observe the effect of hING4 on the proliferation and apoptosis of H460 cells. Results: DNA sequence analysis and PCR indicated that Ad-hING4 were successfully constructed. Ad-hING4 was expressed in lung cancer H460 cells and had obvious cytopathic effect (CPE). Western blotting suggested that hING4 gene caused up-regulation of Bax expression and down-regulation of Bcl-2 expression. Colony formation assay and flow cytometry showed that hING4 inhibited the proliferation of H460 cells and induced apoptosis of lung cancer cells. Conclusion: Ad-hING4 gene was successfully constructed. It inhibited the growth of lung cancer H460 cells in vitro.

Objective: To establish the HPLC fingerprint and multicomponents determination of Potentillae Chinensis Herba, and provide a scientific basis for the improvement of its quality standards. Methods: The separation was performed on a chromatographic Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.1% phosphoric acid as the mobile phase for gradient elution. Volume flow rate was 1.0 mL/min. Column temperature was 25 ℃. Injection was 10 μL and the detection wavelength was 260 nm. The fingerprints of 10 batches of Potentillae Chinensis Herba were established and evaluated by the similarity evaluation system of TCM (version 2004A), which were divided into two categories by clustering analysis. Meanwhile, the content of galic acid, quercetin, and kaempferol was determined. Results: The fingerprint of Potentillae Chinensis Herba was established. There were 12 common peaks in the fingerprint. Galic acid, quercetin, and kaempferol were separated with good linearity relationships (r > 0.999). The average recovery rates of the investigated compounds were 97.44%, 97.64%, and 99.19%, respectively. Conclusion: The established fingerprint and multicomponents determination method of Potentillae Chinensis Herba have strong specificity and good repeatability, which can effectively control the internal quality of Potentillae Chinensis Herba and provide reference for improving the quality evaluation method of Potentillae Chinensis Herba.

Objective: To examine the skeletal factors of Angle's classⅡdivision 1 malocclusion in order to find a reasonable treatment protocol. Methods: The skeletal factors were measured and compared between 86 cases aged 12～25 years with AngleⅡ 1 malocclusion and 86 cases at the same age with normal occlusion. Thirteen measurements were chosen on lateral cephalometric radiograph for the comparison. Results: In the group of maloculsion, some measurements standing for sagittal skeletal pattern were statistically defferent from those in the group of normal occlusion, such as decreased SNB angle and SL linear and increased ANB angle, whereas some were not significently different, such as the SNA angle and PNS-ANS linear; some measurements standing for the vertical skeletal pattern were statistically defferent, such as decreased UM-PP linear and LM-MP linear and increased UI-PP linear, whereas some were not significently different, such as LI-PP linear;some measurements standing for growth pattern of mandibular were statistically defferent, such as decreased SN-MP angle. Conclusion: There are posterior alveolar defficency and mandibular retroposition as well as counterclockwise rotation of mandile in the patients with Angle class Ⅱ 1 malocclusion. The treatment for Angle Ⅱ 1 malocclusion should be to make mandibular move forward and downward in accordance with the characteristic skeletal pattern.

Objective To explore whether there is any abnormality in the tongue position of the open bite. Methods Tongue position was defined as the relative position of its body in the oral proper cavity on profile cephalometric roentgenogram which was enhanced by the iodine oil applied to the tongue surface. The tongue position was compared between open bite group and normal bite group with student's t test to determine the variation of open bite. Results Compared to the normal bite group, the root of tongue was closer to the uvula in the open bite group, and the distance between the dorsum of tongue and the surface of palate was decreased as well as the tip of tongue was nearer to the incisors. Conclusion The open bite's tongue position is characterized by more upward and more forward and this position may be one of the causes of open bite.