Acute Disease ; Child ; Humans ; Respiratory Tract Infections ; virology

China ; Diarrhea ; prevention & control ; virology ; Humans ; Viral Vaccines ; immunology ; Virus Diseases ; prevention & control

Child, Preschool ; Communicable Disease Control ; Diarrhea ; prevention & control ; virology ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Virus Diseases ; prevention & control ; virology ; Virus Physiological Phenomena ; Viruses ; isolation & purification

Actinomycosis ; pathology ; Adult ; Diagnosis, Differential ; Female ; Humans ; Mycoses ; pathology ; Staphylococcal Skin Infections ; microbiology ; pathology ; surgery ; Staphylococcus aureus ; isolation & purification ; Young Adult

Administration, Oral ; Humans ; Rotavirus Infections ; immunology ; prevention & control ; Viral Vaccines ; administration & dosage ; immunology

Objective:To study the characters of perioral muscle p re ssure of the adult patients with skeletal crossbite. Methods:20 patients were diagnosed to be with skeletal crossbite. The perioral muscle press ures on upper and lower central incisors, first molars and cuspids were measured buccally and lingually at rest position. 10 health adults were served as the co ntrols. Results:The perioral force in skeletal crossbite group was larger than that in the control group on the correspondence area(P0.05). In the skeletal cross bite group, the perioral force on the mandibular area was larger than that on th e correspondence maxillary area(P0.05). The muscle pressure on the labial side was larger than that on t he lingual correspondence side in both groups(P

Adult ; Farmer's Lung ; Female ; Humans

Objective To investigate the effect and safety of Ropivacaine mesylate in combined spinal-epid uaral anesthesia of hypogastric operations of elder patient.Methods 90 cases patients scheduled for the lower abdominal surgery were randomly divided into two groups(appendectomy and Oblique inguinal hernia neoplasty).group Ropivacaine mesylate(group A,n=45),group upivacaine hydrochloride(group B,n=45),A,B groups,punctures were selected between L2～L3 ,Hyperbaric solution of Ropivacaine mesylate were injected intrathecaly respectively in two groups,(compounding:group A:0.894% Ropivacaine hydrochloride 1.5ml+10% Glucose lml)and group B;(0.75% Bupivacaine hydrochloride 1.5ml+10% Glucose lml).The two groups after the subarachnoid medication Indwelling catheter to the cephalic in the epidural space.The time of anaesthetic emergence,maintainance,and the anaesthetic level were observed;the analgetic effect was assessed;movement signs of the lower limbs were observed;blood pressure,respiration and SpO2 .postoperative complications were observed.Results There was apparent differentiation between group A which Systob'c blood pressure declined 8.9% ,heart rate decreased 6.7% ,intraoperative nausea came up to 6.7% after anaesthesia and group B which was 28.9% ,20% ,17.8% ,respectively(P ＜ 0.05).The effective time of anaesthetic emergence was(52.0 ±21.5)s and(13.2 ±3.3)min in group A longer than group B was(41.2±18.2)s and(11.3 ±3.6)min(P＜0.01).There was no difference in Sp02,Bromage Score about extreme movement blockage degree(2.3 ±0.7;2.2 ±0.5),the incidence of postoprative headache(0% ;2.2%)between the group A and group B(P ＞ 0.05).Conclusion Ropivacaine mesylate can be used effectively and safetly in combined spinal-epiduaral anesthesia applied in hypogastric operations of elder patients,for cardiovascular stability is superior to bupivacaine.

Objective In order to further understand the bioactivity of sphingosine kinase, high efficient replication defective recombinant adenovirus carrying the wild type SPK gene (SPK WT ) and SPK mutant gene (SPK DN ) were constructed respectively. Methods The SPK genes of interest were subcloned into a shuttle vector pshuttle cmv respectively. Pshuttle cmv SPK WT and Pshuttle cmv SPK DN were linearized by PmeI and transformed respectively into the competent cells of E. coli BJ AD 1 cells which contain adenoviral backbone plasmid pAdEasy 1. The linearized recombinant plasmids were transfected into adenovirus 293 packaging cells respectively,and then recombinant adenoviruses were harvested. We performed the PCR, Western blot and enzyme assays to identify the recombinant adenoviruses. Results The recombinant adenoviruses containing the interest gene and being able to infect ECV 304 cells were obtained. Overexpression of wild type SPK gene in ECV 304 cells increased the endogenous SPK activity, whereas overexpression of mutant SPK (SPK DN ) inhibited intracellular SPK activity. Conclusion Recombinant adenoviral vectors can mediate interesting gene expression in cells.