Objective To investigate the clinical features,etiology and related clinical factors in neonatal anemia.Methods Two hundreds and sixty four infants admitted to the Neonatal Intensive Care Unit between January 2009 to March 2012 were retrospectively analysed with different levels,different age,different gestational age.Results Among 264 patients,the occupancy of blood loss anemia was 54.5 ％.Mild and medium neonatal anemia were more than severe anemia (172 vs 92).Fetomaternal hemorrhage syndrome occurred commonly in severe anemia (16.3％,15/92).Early anemia was more than late anemia(182 vs 82).Hemorrhagic anemia in early anemia (64.3％,117/82)occurred mainly.Late anemia with infection mainly (67.1％,55/82).Fetomaternal hemorrhage syndrome were all early anemia and term to account for a higher proportion (94.4％,17/18);twin-to-twin transfusion syndrome occurred commonly among preterm term infants (96.7％,29/30).Conclusion Blood loss anemia was the main etiological factor resulting in neonatal anemia.The incidence of mild and medium neonatal anemia was quite high.Early anemia was more than late anemia.There might be some differences in the etiology between term and perterm infants.

Objective To evaluate the effect of homemade tirofiban in percutaneous coronary intervention for acute myocardial infarction. Methods Sixty four cases of consecutive acute myocardial infarction were enrolled and divided into two groups: the tirofiban group (n=34) and the control group (n=30). In addition to intravenous heparin, patients in the tirofiban group received a bolus dose of tirofiban (10 ?g/kg) before stenting and followed by a 0.15 ?g/(kg?min) infusion for up to 12-36 hours. The control group only receive routine intravenous heparin therapy before and during PCI. The post operation TIMI blood flow, bleeding events and major adverse cardiac events were observed in the 2 groups. Results A 97.1% of patients in the tirofiban group compared with 76.7% in the control group obtained TIMI grade 2-3 flow respectively. Among them, 91.2% in the tirofiban group but 70.0% in the control obtained TIMI 3 flow. There were no serious in-hospital bleeding complications and MACE. Conclusion Homemade tirofiban is effective in improving the TIMI grade and prognosis of acute myocardial infarction after PCI.

Objective To investigate the relation of inflammation markers to acute coronary syndrome and the clinical significance by observing the changes of P selectin、CRP 、IL 6、TNF ?、ICAM 1 during unstable agina pectoris and acute myocardial infarction(AMI). Methods 45 patients were selected as acute coronery syndrome(ACS)group, including 25 patients with unstable agina pectoris (UAP) and 20 patients with AMI; 25 patients as stable agina pectoris (SAP)and 20 persons as control group. The blood sample of the control group are drawn from venous in the morning, the agina pectoris group are drown from venous in the morning of the second day after hospitalized, however, the AMIgroup are drown from venous at the point of 6 hour、 12 hour、24 hour、48 hour、72 hour after episode. The concentration of CRP is determined by Scatter Turbidimetry , and the concentration of P selectin、IL 6 、TNF ?、ICAM 1 by ELISA. Results (1) SAP vs control ,the levels of P selectin、CRP 、IL 6、TNF ?、ICAM 1 all raise significantly ( P 0.05) ; (4) AMI vs SAP , the levels of P selectin、CRP 、IL 6、TNF ?、ICAM 1 all raise significantly ( P

Objective To investigate the influence of CD4+CD25+ regulatory T cells (Tregs) on the disease progression in MRL/lps mice. Methods Tregs were separated by using magnetic beads from splenic cells of MRL/lps mice and BALB/c mice, and concentrated. Twenty-four MRLAps mice were equally divided into 3 groups, test group 1 injected with Tregs from MRL/lps mice, test group 2 injected with Tregs from BALB/c mice, and control group injected with physiological sodium chloride solution. Three weeks later, the levels of urine protein as well as serum anti-dsDNA antibody were determined; subsequently, the mice were sacrificed followed by histopathological and immunopathological examination of renal tissue. Results A significant decline was observed in the test group 1 compared with the test group 2 and control group in the urine protein score (10.63 ± 4.17 vs. 20.00 ± 5.35 and 18.75 ± 8.34, both P < 0.05), serum anti-dsDNA antibody level (5.36 ± 2.40 pg/ml vs. 9.57 ± 1.97 pg/ml and 10.75 ± 3.98 pg/ml, both P < 0.05), glomerular sclerosis index [(32.00 ± 12.09)% vs. (45.50 ± 13.68)% and (47.50 ± 10.78)%, both P< 0.05], and immunofluorescence intensity of IgG immune complex in renal tissue (1.88 ± 0.99 vs. 2.88 ± 0.64 and 2.75 ± 0.71, both P< 0.05). No significant difference was noted in renal tubule interstitial impairment index between the 3 groups (4.63 ± 1.92, 6.00 ± 1.07 and 5.75 ± 1.28, all P> 0.05). There was no statistical difference between the test group 2 and control group in terms of any of the above parameters (all P > 0.05). Conclusions Injection of Tregs from homologous mice could significantly down-regulate proteinuria degree, serum anti-dsDNA antibody level, glomerular sclerosis index and IgG immune complex level in renal tissue, and thereby decelerate the progression of renal impairment in MRL/lps mice.

The successful establishment of animal models of cardiac arrest-cardiopulmonary resuscitation (CA-CPR) undoubtedly provided an important basis for exploring the method of cardiopulmonary resuscitation (CPR) and advanced cardiovascular life support (ACLS). However, pathophysiology varied with the etiology of cardiac arrest (CA). Therefore, preparation of similar animal models according to etiology was the basis for pathophysiological changes research. Compared with other animals, the rabbits had both the advantages of large and small animals, so they became common research object for the CA-CPR model. This paper reviewed the common methods of animal models of CA-CPR in rabbits. In this review, the methods, criteria, advantages, disadvantages and precautions of each model were analyzed, which would provide useful reference for CPR researchers.

Objective To discuss magnetic resonance fat suppression technique,such as Chemical Shift Selective and Short TI Inversion Recovery Sequence,and its clinical value.Methods 73 cases undergoing MRI fat suppression during March 2008 to July 2008 were adopted as the subjects,with such site examined respectively as the skull,basalis,nasopharynx,neck,bones and joints as well as abdominal pelvis.Comparative study of image quality was performed to determine the di-agnostic value of MRI fat suppression technique.Results Of the all cases,7 ones suffered from skull lesions,6 ones from eye diseases,11 ones from abdominal pelvic diseases,4 ones from liver diseases,4 ones from pancreas disorders,and 8 ones from pelvic diseases.Meanwhile,of 10 cases of basalis diseases,there were 8 ones of nasopharyngeal carcinoma and 2 ones of oropharynx disorder.Of 16 cervical disorders,there were 6 ones of neurogenic tumor,3 ones of leucoma,5 ones of metastatic tumor and 2 ones of lipoma.Of 15 cases of vertebral disorders and osteoarthropathia,there were 8 ones of bone contusion,3 ones of metastatic tumor,3 ones of angeioma and 1 case of lipoma.Fat suppression technique,with the lesion edge outlined clearly and the lesions containing adipose tissue identified,provided more information than regular MRI.Conclusion The fat-suppression technique can significantly improve image quality,enhance disease diagnosis rate,and thus is an important technology in MRI examination.

OBJECTIVE:The content of resorcinol in compound resorcinol lotion was determined by dual wavelength spec?trophotometry.METHODS:Absorbance was determined by using water as the solvent at the wavelength273.5nm and266.1nm.RESULTS:The results showed that the standard curve of resorcinol concentration ranging from20.32?g/ml to60.96?g/ml was in correspondence with Beer’s law.The absorbance difference and the concentration showed a good linear relationship(r=0.9998).The mean recovery of resorcinol was(99.59?0.45)%(n=9).CONCLUSION:This method is simple,quick and ac?curate.

Objective: It was to investigate the content alteration of the tannins in cibot rhizome and its processed samples. Method: The casein method was used to determine the content of tannins. Results: The content of tannins decreased after the medicinal material was processed. Conclusion: Processing may decrease the content of tannins in cibot rhizome. The raw was better than the other processed samples if the tannins were used as active constituents.

Objective:To explore the treatment effect of A-NK cells on the subcutaneously transplanted tumor of tongue squamous cell carcinoma in nude mice.Methods:Tca8113-Tb cells were subcutaneously transplanted into nude mice,14 tumor bearing mice were randomly assigned to 2 groups(n =7).The mice in control group were injected with normal saline,those in the experimental group were injected with A-NK cells.All animals were killed 33 days after tumor cell transplantation,The tumor volume and weight of the mice were measured and compared.Results:A-NK cells significantly inhibited tumor growth in nude mice in terms of the volume (P ＜0.05).After 15 -33 d of treatment the tumor weight(g)in the treated and control mice were 0.96 ±0.38 and 3.74 ±1.22 re-spectively(P ＜0.05).Conclusion:A-NK cells can inhibit the growth of Tca8113-Tb cell induced tumor in nude mice.

Objective To analyze the changes of Blood Cell Count and D-dimer concentration in lung cancer patients with thrombosis.Methods ①554 cases of patients with lung cancer were included between January 2012 and November 2013,75 patients with other diseases which lead to high coagulation state were excluded,blood cell count and D-dimer concentration were compared between 64 lung cancer patients were the cases with thrombosis with 415 lung cancer patients were the con-trol group without thrombosis.②Color doppler ultrasonography was taken as the golden standard,receiver operating charac-teristic curves (ROC)were drawn for indexes which had significance in method.Results ①Compared with lung cancer pa-tients without thrombosis,lung cancer patients without thrombosis had decreased red blood cell count (RBC),increased white blood cell count (WBC)and D-dimer.And there were no significant change in platelet (PLT).②The area under curve (AUC)of ROC in RBC,WBC and D-dimer were 0.662,0.637 and 0.896,respectively.By thecut-off values of RBC>4.06× 1012 L,WBC<5.37×109/L and D-dimer<4.02 mg/L,the negative predictive values of RBC,WBC and D-dimer for diagno-sis of lung cancer patient with thrombosis were 93%,93% and 96%,respectively.By the cut-off value of D-dimer>4.02 mg/L,the positive predictive value of D-dimer for diagnosis of lung cancer patient with thrombosis was 6 6%.Conclusion RBC,WBC and D-dimer were related with the progress of thrombosis in lung cancer patients.RBC,WBC and D-dimer have good negative prediction effect on lung cancer patients with thrombosis.D-dimer had positive effect for diagnosis of lung cancer with thrombosis.