The gastric cancer is one of the most common malignant tumors in human. The current studies demonstrated that Helicobacter pylori(HP) is one important promotion factor in gastric cancer development. This text aims to overview three aspects including toxic factors and function mechanism of HP in gastric cancer,gene susceptibility of the host and environmental factors studying by domestic and international scholars.

Animal models using for research the cancer matestasis should be entirely similar to the clinical pathological process in patients.Currently,two kinds of animal models with genetic engineering and human tumor xenografts are available in laboratory,which provide a possible research tool to investigate the mechanism of tumor metastasis and explore the experimental treatment.In these models,the techniques including how to select the cell lines and using real-time imaging detection are very important factors.

Collaterals is a term in Traditional Chinese Medicine (TCM) in human body.It is the branch of the Channels and Collaterals that consists of DaLuo,XiLuo,ChanLuo,SunLuo and FuLuo,etc.Its function are transporting qi and blood,connecting zang- fu organs and limbs, as well as the upper and the lower,the interior and the exterior, and regulating all parts of the body.Collaterals disease is the disorder of the function of transporting qi,blood and body fluid,and the pathologic metabolite interference in Collaterals due to various agents. There are many therapy such as communicating Collaterals with Pungent Flavour and Moistening,Pungent Flavour and Worm, Pungent Flavour,Clearing away Heat and promoting the dispersing function, Aromatic,Clearing away Heat,Clearing away thrombosis,Clearing away sputum,insect and ants therapy,promoting qi, moistening and promoting,Clearing and moistening,worming and moisting and Softening liver, etc.

Objective To discuss the pe rc utaneous laser disc decompression (PLDD) therapy by adopting the Nd: YAG Laser C L-50 Device under X-ray monitor supervision. Methods A total of 378 cases were included in this study. There was 198 males and 180 fe males, the age ranged from 19 to 76 years, with the average of 43.9 years, and the course of diseases from 1 month to 16 years. 17 cases of lumbar interverteb ral disc prolapse were diagnosed between L2 and L3, 35 cases between L3 and L4, 140 cases between L4 and L5, and 116 cases between L5 and S1. 60 cases with two intervertebral disc prolapses at the same time, and 10 cases with three interve rtebral disc prolapses at the same time. A total of 458 intervertebral-dis c operations were conducted with PLDD. All the cases selected had been examined by CT or MRI, and their clinical symptoms and physical signs were in compliance with the diagnosis standards for prolapse of lumbar intervertebral disc. Results After PLDD operation, 268 cases were cured within 1 month with a cure rate of 70.90%; 259 cases were cured in 3 months with a cu re rate of 68.52%, 260 cases were cured in 6 months with a cure rate of 68 .25%. Stable results were found with curing satisfaction rate, effective rate and failure rate. Conclusion X-ray guided PLDD therapy is an ideal non-operative therapy for curing lumbar intervertebral disc prolap se with micro-trauma, little pain, quick recovery and good effect. PLDD has ac hieved encouraging effects both in its recent clinical outcome and long term eff ect as well.

The eight scalp needles, founded by Professor QIN Liang-fu, and its clinical experience for treatment of Parkinson's disease (PD) are introduced. Based on his years of clinical experience, it is proposed by Professor QIN that the Governor Vessel is mainly for miscellaneous disease and disease of limbs. Combined with distribution of cephalic motor region and meridian, an acupuncture treatment plan that is full of innovativeness is proposed, which is called Qin's eight scalp needles. It includes bilateral Fengchi (GB 20), Shuaigu (GB 8), Toulinqi (GB 15) as well as Yintang (GV 29) and Baihui (GV 20), mainly for treatment of nervous system diseases, such as PD and multiple sclerosis and so on. Besides, some outpatient cases are introduced to explain that eight scalp needle could alleviate the progression of PD, improve patients' motor, cognitive and affective disorders, reduce the suffering of patients, and improve the patient's quality of life.
Acupuncture Points ; Acupuncture Therapy ; history ; methods ; China ; Female ; History, 20th Century ; History, 21st Century ; Humans ; Male ; Middle Aged ; Parkinson Disease ; history ; therapy ; Scalp

One-day-old unvaccinated chicks were inoculated with serotype I virulent strain GA and vaccine strain CVI988, serotype III vaccine strain HVT o f Marek's disease viruses(MDV). By using an indirect immunofluorescence assay of suspensions of peripheral blood mononuclear cells(PBMC) with monoclonal antibo dies(MAb) BA4 and BD8 specific to glycoprotein B of MDV, The dynamic course s of vir emia levels for each of 3 strains from 4 days to 45 days postinfection (PI) were determind. Viremia for virulent strain GA-infected chickens were detected from 4 days PI until days before death of infected chickens, it got its peak at abou t 14 days PI. Viremia in serotype 1 vaccine CVI988-inoculated chickens were mea sured between 4 to 22 days PI, it could be detected from 4 days PI and ended at 18 days PI, it got its peak at 8 days PI. For serotype 3 vaccine HVT-inoculated chickens, viremia could be detected beween 4 to 14 days PI, its peak happened a t about 6 days PI. Such IFA with MAbs BA4 and BD8 could be used to evaluate qu al ity of vaccination process in chicken flocks. The differential dynamic courses o f viremia for different strains may be used to diagnose virulent MDV infection i n vaccinated flocks. The viremia levels measured by IFA with the MAbs and co-cu ltivation assay for plague forming unit(PFU) were compared. The results indicate d that IFA assay of PBMC suspension was more sensitive and specific than co-cu ltivation assay, i.e. viremia levels measured by IFA were 30 to 100-fold higher than that by plague-forming assay in CEF for the same sample.

Escherichia coli ; classification ; Escherichia coli Infections ; epidemiology ; microbiology ; Germany ; epidemiology ; Humans ; Pandemics

Female ; Humans ; Infant ; Mastocytosis, Systemic ; diagnosis ; pathology ; therapy

Anesthesia is the most crucial part of clinical surgery in modern medicine,and it is highly risked due to complications of general anesthesia.Researches have proved that intraoperation monitoring depth of anesthesia efficiently reduced the dosage of anesthetics and led to earlier recovery,so that the risk of anesthesia has been minimized.Currently,the research on depth of anesthesia monitoring methods has become an international hotspot.This paper reviews the methods for monitoring depth of anesthesia,including bispectral index,auditory evoked potential,Entropy and heart rate variability,and highlights advantages,disadvantages of these methods and the relationship between these methods.This paper also introduces the relationship between heart rate variability and the depth of anesthesia monitoring,the method for analyzing heart rate variability in both time domain and frequency domain,which laid a theoretical foundation for the research on monitoring depth of anesthesia.

BACKGROUND:As a material to repair osteochondral defect,the acellular cartilage matdx has attracted more and more researchers'attention,however,there is no comparison between two ways of decelluladzation.OBJECTIVE:To test trypsin and Triton X-100 methods for their potential of cell removal.DESIGN,TIME AND SETTING:Controlled observation was performed at the Central Laboratory of Orthopaedics in the Second Hospital of Shanxi Medical University between March and July in 2008.MATERIALS:Fresh knee and hip articular cartilages of pig were purchased from the market.METHODS:Porcine articular cartilages were treated with either 0.25%trypsin or 0.25% Triton X-100 respectively for decelluladzation,while fresh untreated articular cartilage served as control.MAIN OUTCOME MEASURES:①General observation on the morphology of acellular articular cartilage.②Immunohistochemistry and scanning electron microscopy were used to determine the effect of two decellularization methods on the extracellular matrix collagen.③Ten articular cartilage leaflets in each group were measured for breaking strength and percentage elongation through loading,unloading and breaking tests.RESULTS:①By use of trypsin and Triton X-100 methods,the acellular matrix had no significance difference in the morphology compared with untreated cartilage,only presenting slightly white color and feeling less soft.②lmmunohistochemistry and scanning electron microscopy results showed that,trypsin and Triton X-100 methods achieved complete decelluladzation.Trypsin had no significant influence in extracellular matrix and cartilage leaflets microstructure;Triton X-100 caused some tiny structural alterations,such as more collagen and disordered structure.It is possibly that Triton X-100 damaged extracellular matrix.③No significant difference was identified between untreated and trypsin,Triton X-100 groups in breaking strength and percentage elongation.CONCLUSION:Trypsin and Triton X-100 all achieve complete decellularization.but trypsin uses short time and low costs with well preservation,while Triton X-100 with long procedure and slight damage to collagen structure.They all have no significance influence to the mechanics characteristics of articular cartilage.