Objective:To explore the effect of two kinds of cardioplegia on cardiomyocyte apoptosis and the related protein expressions in rat hearts. Methods:24 Wistar rats were randomly divided into 3 groups(n=8):Control group,STH-2 group and TTX group. Isolated rat Langendorff and Neely working heart models were estabished. The changes in cardiomyocyte apoptosis were detected by TUNEL.The expression of Bcl-2 and Bax protein were detected by immunohistochemical assay in cardiomyocyte. The expression of Bcl-2 protein in cardiomyocyte was detected by Western blot. Results:Apoptosis index(AI) in STH-2 group(11.20?0.79)% was significantly higher than that in TTX group(6.92?1.10)% and Control group(1.34?0.23)%(P

OBJECTIVE:To study the antitumor activity and antioxidative action of glycosaminoglycan from scallop skirt(SS-GAG).METHODS:The effects of different concentration of SS-GAG on the proliferation of four tumor cell lines(HeLa,LOVO,A549,and U251)which had been cultured in vitro were observed by MTT.The effect of SS-GAG on mice within 30 d after innoculation of sarcoma 180 was observed.Multi-kind of biochemistry methods including xanthine oxidase method and thiobarbituric acid colorimetric method were used to study the effect of SS-GAG on total antioxidation activity,activity of SOD,and level of MDA in tumor-bearing mice,which were compared with those in control group.RESULTS:As compared with control group,SS-GAG significantly inhibited the growth of HeLa and U251 cell lines,inhibited the growth of LOVO and A549 cell lines to some degree,significantly prolonged the survival time of the mice with S180 ascites tumor,enhanced the total antioxidant activity and the SOD activity and decreased MDA content in serum(P

Objective To explore the advantages and disadvantages of the double lumen endotracheal intubation and single -lumen endo-tracheal intubation and continuous carbon dioxide insufflation in thoracoscopic esophagectomy .Methods The clinical data of 90 patients in our department of thoracic surgery after thoracoscopic esophagectomy from January 2014 to April 2015 were analyzed .All patients were divid-ed into single-lumen endotracheal intubation (group A)and double lumen endotracheal intubation group (group B).The endotracheal intuba-tion time,operation time,incidence of pulmonary infection,intraoperative and postoperative PaO2,PaCO2,incidence of anastomotic fistula, hospitalization expenses ,length of hospital stay and the incidence of postoperative chylothorax between two groups were compared .Results The difference in intraoperative PaO2,PaCO2,incidence of pulmonary infection,endotracheal intubation time,operation time,hospitalization days and the hospitalization cost between two groups were statistical significance .The difference of the rest index between two groups were no statistical significance.Conclusion Group A has certain advantages in perioperative management ,hospitalization cost and so on,but has disadvantages in perioperative hypoxemia and carbon dioxide retention and acid -base balance disorders .

OBJECTIVE:To objectively evaluate the conditions of the import d rugs so as to provide a basis for the rea?sonable regulating of drug use structure.METHODS:The data about the sales sum,drug variety,pharmaco-categorization,antimicrobial drugs and so on of drugs used in10hospital of Shenyang during2001～2003were analyzed statistical?ly.RESULTS:The variety kinds and the proportion of sales sum to the total of the import drugs have decreased;The antimi?crobial drugs remained a dominating place in terms of drug variety and sales sum for the3years in succession;The drugs that dominated the first10places in terms of sales sum accounted for above70%of the total sales sum of antimicrobial drugs for the3years.CONCLUSION:The import drugs have been replaced by drugs from the joint venture and the domestic markets,it is necessary for the concerned institution to take effective measures to tackle the problem of over-centered drugs use of antimi?crobials.

Objective To investigate the efficacy of clinical case-based PBL in clinical practice in department of urology.Methods Medical students admitted by our clinical medical school in 2006 and 2007 were involved.Fifty-six students of 2007 grade were chosen as study group while 49students of 2006 grade as control group.Students in study group were taught by clinical case-based PBL method while those in control group by traditional method.All results were analyzed by SPSS 11.0.Results The scores in study group were higher than those in control group with statistical differences (82.84 ± 12.35 vs.72.95 ± 11.13 ; t =4.29,P ＜ 0.01).Students in study group showed more interests in practice lessons.Teachers were more satisfied with the overall performance of students in study group.Conclusions Clinical case-based PBL can increase students' overall performance such as better coordination,better clinical skills and better open-minded clinical case solution.

Objective:To explore the prognostic value of N-terminal pro brain natriuretic peptide (NT-proBNP)as-sessing prognosis in patients with non ST elevation myocardial infarction (NSTEMI).Methods:Clinical and follow-up data of 96 NSTEMI inpatients from our hospital were retrospectively analyzed.According to their outcomes, they were divided into no event group [n=46,no major adverse cardiovascular events (MACE)occurred]and event group (n=50,MACE occurred).Levels of NT-proBNP,total cholesterol (TC)and low density lipoprotein choles-terol (LDL-C),left ventricular end-diastolic diameter (LVEDd),mitral early/late diastolic peak flow velocity (E/A)and left ventricular ejection fraction (LVEF)were measured and compared between two groups.Single-factor linear analysis and multivariate Logistic regression analysis were used to analyze relative factors of MACE incidence rate,ROC curve was used to analyze the best predictive value of NT-proBNP.Results:Compared with no event group,there were significant rise in NT-proBNP level [(3157.5±102.4)pmol/L vs.(4309.6±214.6)pmol/L]and LVEDd [(58.1±5.5)mm vs.(74.3±6.8)mm];and significant reduction in LVEF [(71.8±4.1)% vs.(49.5 ±3.9)%]and E/A [(0.84±0.18)vs.(0.62±0.12)]in event group (P <0.05 all).There were no significant difference in TC and LDL-C levels between two groups (P >0.05 both).Multivariate Logistic regression analysis in-dicated that NT-proBNP was an independent predictor for MACE (OR=1.003,P =0.009).Survival rate of those with plasma NT-proBNP≤4390 pmol/L was significantly higher than those with NT-proBNP >4390 pmol/L (OR=5.028,P =0.021).Conclusion:Plasma N-terminal pro brain natriuretic peptide can independently predict progno-sis of patients with non ST elevation myocardial infarction.

Objective To investigate the optimum formulation and preparation process of Yuxiawenya dispersible tablets.Methods The formula of Yuxiawenya dispersible tablets were optimized by orthogonal design in terms of dispersible uniformity test.Results The tablets were prepared by moist granulation method with 1% polyvidone k30(PVPK30) ethanol solutionsas as moistening agent,microcrystalling cellulose(MCC) as diluents,and sodium croscarboxymethyl starch(CCMS-Na) as disintegrant.The disintegration time of dispersible formulation was

Objective To investigate the application of Multi-tumor markers Protein Chip Diagnose System in the diagnosing of ovarian cancer. Methods Using the Multi-tumor markers Protein Chip Diagnose System to determine and analyze the concentration values of 12 tumor markers(AFP, CEA, NSE, CA125, CA153, CA242, CA199, PSA, f-PSA, FER, ?-HCG and HGH) in the sera of 53 malignant ovarian cancer patients, 12 ovarian cysts patients and 98 normal persons. Result At least one kind of tumor marker was found higher in 44 sera of the 53 malignant ovarian cancer patients(positive ratio is 83 0%), in 7 sera of the 12 ovarian cysts patients(positive ratio is 58 3%) and 2 sera of the 98 normal persons (negative ratio is 97 9%). NSE、HGH、PSA and f-PSA were first found higher in the sera of ovarian cancer patients. Conclusion The application of Multi-tumor markers Protein Chip Diagnose System in the diagnosing of cancer not only greatly reduces analysis of tumor markers concentration in serum and left the accuracy of diagnosis but may lead new discoveries about the tumor markers.

This review summarized the current progress in the pathogenesis of leptin-mediated liver disease including fatty liver, hepatitis,fibrosis or cirrhosis. Analysis of leptin effect on activation of hepatic Kupffer cells and hepatic stellate cells was made and molecular mechanism and signal pathways were also discussed.

Polycystic kidney disease is a common human congenital hereditary disease, which can be divided into autosomal dominant polycystic kidney disease and autosomal recessive polycystic kidney disease by genetic methods. In addition to the kidney changes, polycystic kidney disease may also involve multiple body organs and seriously endanger human health. In recent years, studies on etiology and pathogenesis ( especially molecular biology), diagnosis and treatment of polycystic kidney disease have progressed by leaps and bounds. This paper briefly summaries the research status and development of the most common autosomal dominant polycystic kidney disease.