Objective To discuss endodontic surgery,such as the medical treatment effect of the fixation and reparation of tooth after root amputation and hemisection and prepare and provide medical basis for further application of the treatment. Methods Choosing fifty patients with hemisection or extirpating of root of tooth. After filling out the remaining root of tooth with silver and mercury, then make the operations of cutting tooth or extirpation of root of tooth in the condition of local anesthesia. For two to three months after the operation, on the basis of the remaining root of tooth sad next tooth with some body deformities, to make the operation of the united fixation and reparation. Then con-tinue to observe the treatment effect for two to three months. Results It has a good effective treatment on the united fixation and reparation of the remaining root of tooth united with next tooth with some body deformities after the opera-tions of root amputation and the hemisoction. The patients'chewing strength is strong and it is easy to clean and less to get local anesthesia which is often occurred after the operations. Conclusions Molars remained after hemisection of tooth or root amputation cannot be easily extirpated, so we may consider to made the operations of united fixation and reparation with next tooth. The fixed bridge has fixing and protection functions to the remaining mot of tooth and can improve chewing power.

Objective: To standardize the method for morphine dose titration and investigate the synergistic effect of dextromethorphan with morphine on cancer pain. Methods: Eighty six adult patients with severe cancer pain were selected. The experiment was divided into two stages: the stage of morphine dose titration and random double-blind contrast test. At the stage of morphine dose titration the patients were administered morphine 5 mg/4 h at baseline and given "as needed" morphine palliative treatment at 10% total daily dosage when abrupt pain occurred till the patients gained stable pain. At the stage of random double-blind contrast test the dosage of morphine was reduced by 30% and dextromethorpban or its analogue tablets (dose ratio 1: 1) was added. Morphine dose titration was performed again till pain relief. Results: During the stage of morphine dose titration the dose of morphine was increased from the second day. The dose of morphine was stabilized on the fourth day. Morphine was given to the patients at the stable dose continuously. The minimum pain-relieving dose of morphine was 30 mg and the maximum dose was 160 mg. The pain intensity of the patients began weaker from the third day and kept stable during the treatment. The life qualities of the patients were obviously improved. During contrast test stage the total dose of morphine was reduced in both groups. The dose of morphine was reduced a little more after addition of dextromethorphan compared with the control group. But there was no significant difference between the two groups. The pain intensity kept stabilized during the treatment. Conclusion: Standardized morphine dose titration achieved satisfactory effects on cancer pain. Addition of dextromethorphan tended to reduce the total dose of morphine.

Objective To study expression of matrix metalloproteinase‐9(MMP‐9) protein and its mRNA in breast cancer and e‐valuate its significance in the occurrence ,development and metastasis of breast cancer .Methods The protein expression of MMP‐9 breast cancer were detected by using immunohistochemistry and the expression of mRNA were detected by reverse transcription polymerase chain reaction(RT‐PCR)Results The positive rate of MMP‐9 protein expression in 56 cases of breast cancer was 69 .6% (39/56) ,while in benign breast diseases was 20% (6/30) ,which were significantly different(P<0 .05) .The levels of MMP‐9 mRNA were significantly higher in the patients with breast cancer than those in benign breast diseases(P<0 .05) ,which were 0 .914 2 ± 0 .108 1 and 0 .379 4 ± 0 .0428 respectively .Conclusion The MMP‐9 protein and mRNA expression in human breast cancers are positively correlated with the stage and lymph node metastasis .Expression of MMP‐9 could be used as an indicator for the ability of invasion and metastasis of breast cancer .

During epidemiology teaching,it is important for teachers to use person-based teaching method according to different specialty bachelors to improve the teaching quality.

ObjectiveTo observe the effect of family rehabilitation plan (FRP) on motor function of stroke patients with hemiplegia.Methods64 patients were randomly divided into the FRP group (32 cases) and control group (32 cases). The patients in the FRP group critically followed the schedule and activities scheduled on FRP and were assessed and guided in out-patient department every two weeks. The patients in the control group made schedule and carried on activities by themselves. Motor function was assessed with Fugl-Meyer Assessment (FMA) and modified Barthel index (MBI) respectively.ResultsMotor scores of the patients in two groups significantly increased after treatment ( P<0.001), and that of the FRP group was significantly better than that of the control group ( P<0.001).ConclusionThe FRP can significantly improve motor function of stroke patients with hemiplegia.

Objective To explore the behavioral patterns of middle school students and provide basis for unintentional injury prevention in these students.Methods 171 injury students and 171 normal students as controls were evaluated by Youth Self-Report(YSR),and mean scores were compared between the two groups by t Test.Logistic regressions were performed to analyze the relationship between injury students and behavioral problems.Results The rate was higher in injury group(15.79%) than in control group(8.19%).The score of injury group of boys was higher than thar of control group(t=2.942,P=0.004);the score of injury group of girls was higher than thar in control group(t=3.766,P=0.000).Multi-factor Logistic regression analysis showed that attention-deficit(OR=5.376),the problem of thinking(OR=3.672),social problems(OR=2.204) and friendly fellows' action was a risk factor.Conclusions There is a significant effect of the behavior of students and fellow students on the injury to students.

The cell survival rate and the chromosomal aberration induced by N-methyl-N-nitro-N-nitriosoguanidine (MNNG), mitomycin C (MMC) and ethyl methanesuiphonate (EMS) in V79 cells and its ?-ray-sensitive mutants (irs5 and mil) were observed in this study. The concentrations of mutagens to determine the survival rate were arranged at 2.5~ 30 ?mol/L in MNNG, 1~20?mol/L in MMC and 2~ 16 ?mol/L in EMS, respectively. The cell survival rates and the cell clones of irs5 were higher than that of the V79 cells and irs11; and of irs11 were significantly lower than that of V79 cells and irs5. The result of IC50 of mutagens to determine the chromosomal aberration was similar to the cell survival rate. The type and frequency of chromosomal aberration induced by MNNG, MMC and EMS in irs5 was same as in V79 cell; and there was a dose-response relationship between them. The spontaneous aberration frequencies in irsS and V79 cells were lower than 4%, however, in irs11 was higher than 10%. Data suggests that the cellular biological characterization in irs5 is steady, and further study to certain irsS whether it will be a new mutant for the mutagenicity or carcinogenicity assay or not.

This study investigated the expression of hemeoxygenase-1 (HO-1) in rats with acute lung rejection and its implication. A valid rat orthotopic left lung transplantation model (SD rat-->Wistar rat) was established by using an improved three-cuff anastomosis technique. The rats were divided into control group, CoPP (HO-1 inducer)-treated group and ZnPP (HO-1 inhibitor)- treated group. The severity of acute rejection was graded on the basis of the morphologic changes of the lung samples stained with HE. The expression of HO-1 protein in lung tissue was detected by using immunohistochemistry and Western blot, and HO-1 mRNA activity was assayed by RT-PCR. The results showed that the expression of HO-1 protein was significantly increased with the acute rejection grading in rats (P<0.01). As compared with control and ZnPP-treated groups, the severity of acute rejection was not alleviated and the grade not reduced significantly in CoPP-treated group (P>0.05). It was concluded that HO-1 protein might be involved in the pathological process of post-graft acute rejection. The expression of HO-1 protein was increased gradually with aggravation of acute rejection, and HO-1 protein might be used as an index to monitor acute rejection after lung transplantation.
Graft Rejection/*enzymology ; Heme Oxygenase (Decyclizing)/genetics ; Heme Oxygenase (Decyclizing)/*metabolism ; Lung Transplantation ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rats, Sprague-Dawley ; Rats, Wistar

Objective To study the clinical significance of MR imagings features in the diagnosis on central neurocytoma ( CNC ) . Methods From January 2010 to December 2012,9 patients with CNC were analyzed and examined by MRI before surgery,then received postoperative pathology examination. Results CNC were in the left lateral ventricle of 9 patients, closely linked with the Monro hole, of which 4 cases were in the first 2/3 of central ventricle of the left lateral ventricle,3 cases in the septum pellucidum and growth to bilateral ventricles,2 cases of infiltrating in the septum pellucidum and base side adhesion. For CNC,MRI signal was not uniform,solid part T1WI showed equal or slightly low signal,multiple cysts and signal cord like structure with the ventricular wall and septum pellucidum adhesion. By contrast-enhanced CT scan,there were the solid part heterogeneous obvious enhancement in 4 cases,moderate and slight uneven enhancement in 3 cases and 2 cases respectively. Uniform size,round or oval cells were showed by HE staining,and the synaptophysin was positive in 6 ca-ses by immunohistochemical staining,positive expression of glial fibrillary acidic protein in 3 cases. After operation,3 patients were lost to fol-low up,for 6 cases were followed up,survival of 2 cases in 3 years,4 cases in 2 years. Conclusion The results suggest that MRI display is located near the lateral ventricle central Monro hole before and lesions suggestive of CNC in young patients. For most CNC,synaptophysin has positive expression by immunohistochemical staining.

Objective To discuss the expressions and clinicopathologic significances of insulin-like growth factor Ⅱ mRNA binding protein 3 (IMP3)in squamous intraepithelial lesion (SIL)and cervical squa-mous cell carcinoma (CSCC)before and after the therapy of radiation and chemotherapy.Methods The expressions of IMP3 in 80 cases of CSCC,90 cases of SIL (60 cases of HSIL,30 cases of LSIL)and 30 cases of cervicitis were detected by immunobistochemistry.The relations between IMP3 and clinicopathological cha-racteristics of CSCC were analyzed.Results The expression rates of IMP3 in CSCC,HSIL,LSIL and cervici-tis were 86.3%(69 /80),78.3%(47 /60),33.3%(10 /30)and 0(0 /30),and the difference among the four groups was statistically signicant (χ2 =87.01,P <0.01).The positive expression rate of IMP3 declined by radiation or chemotherapy (60.0% vs.85.0%,χ2 =5.79,P =0.013).The expression of IMP3 was related with lymph node metastasis (χ2 =3.97,P =0.046),differentiated degree (χ2 =5.95,P =0.018),clinical stage (χ2 =5.82,P =0.016)and invasion depth (χ2 =5.73,P =0.017).There was nothing to do with age (χ2 =0.11,P =0.745).Conclusion IMP3 expresses excessively in CSCC,and is associated with pathologi-cal grade and invasion progress.Radio-chemotherapy can reduce the expression of IMP3.