To investigate the features of CT, ultrasonography and fundus fluorescein angiography (FFA) of morning glory syndrome, the data on CT, A/B-scan ultrasonography and fundus fluorescein angiography (FFA) were retrospectively analyzed in 8 cases of morning glory syndrome (MGS). Among those cases, 6 were examined with CT, 4 with FFA and 8 with A/B-scan ultrasonography. Results showed that the characteristics of CT, A/B-scan ultrasonography and FFA in MGS included: (1) The attachment spot of optic nerve became thin and vitreous body protruded to the posterior wall of eyeball with a spherical shape on CT image; (2) in the early period of FFA, hypofluorescence appeared on the optic, the abnormal arteriae and veins around the optic papilla were displayed clearly and in the late period, optic disc was stained with fluorescein; (3) on B-scan ultrasonogram, the vitreous cavity extended to the posterior pole and optic papilla, and projected to the basal part of muscle cones and thus the posterior part of vitreous cavity looked like an upside-down bottleneck. Sometimes the echogenic band of retinal detachment could also be seen. On A-scan ultrasonogram, both vitreous cavity and bottleneck showed no ultrasonic echoes and presented a base line without any evident wave crest. It is concluded that CT, A /B-scan ultrasonography and FFA could show the imageological features of MGS from different aspects, which helps clinicians to differentiate it from other diseases such as optic disc coloboma. CT and A /B-scan ultrasonography, in particular, are considered to be reliable imageological methods for the accurate diagnosis of MGS and are superior to the traditional techniques.

Objective To explore the pathologic changes of the opening blood-brain barrier(BBB) in the early stages of 50% TBSA scalded rabbits.Methods Ten male rabbits inflicted with 50% TBSA of Ⅲ degree were randomized into five groups: control,postburn 1 h,2 h,3 h,4 h.Brain tissues were isolated at different phases and observed in gross,by microscope and transmission electron microscope.Results Endothelial cells swelled and BBB began to open in 2 h after scald.The tight junction of endothelial cells became vague and the opening of BBB increased as lesion time prolonged.Conclusion As the lesion time prolonged,the injury of endothelial cells became more serious and the opening of BBB progressed,which resulted in the formation of brain edema.

In recent years, there is increasing evidence that the pathogenesis of Parkinson's disease (PD) may involve the more generation of reactive oxygen species, and investigations on patients have shown that PD is under a status of oxidative stress. The defense against the toxic effects of reactive oxygen species is an essential task within the brain. An important component of the cellular detoxification of reactive oxygen species is the antioxidant glutathione. Consequently, it seems reasonable to propose that increase of brain concentrations of glutathione including glutathione analogs or precursors could be very effective in diminishing the cumulative effects of oxidative damage, and have been investigated as potential therapeutic targets in the treatment and prevention of PD or other neurodegenerative disease.

Growth arrest special 5 (GAS5) is a common long non-coding RNA (lncRNA).Recent studies find that lncRNA GAS5 is down-regulated in various tumors,such as breast cancer,cervical cancer,lung cancer,gastrointestinal cancers and urologic cancers.GAS5 is closely related to the development,metastasis and prognosis of cancers,which becomes a new hotspot in cancer research.

To investigate the effects of pyrrolidine dithiocarbamate (PDTC) on the proliferation and PCNA (proliferating cell nuclear antigen) expression of cultured human retinal pigment epithelium cells, human retinal pigment epithelium cells (RPE) were cultured from normal adults who, died accidentally. The effects of PDTC on the proliferation of RPE cells were examined by using methyl thiazlyl tetrazolium (MTT) assay. The effects of PDTC on the PCNA expression of RPE cells were immunohistochemically examined by employing biological image analysis system (BIAS). After treatment with PDTC of various of concentration ranging from 0.062 to 1 g/L for 24 h, or concentrations ranging from 0.031 to 1 g/L, the proliferation of RPE cells decreased in a dose-dependent manner. After treatment with PDTC of concentration varying from 0.062 to 1 g/L for 24 h, the PCNA expression was also suppressed in a dose-dependent manner. It is concluded that PDTC can inhibit the proliferation of RPE cells in vitro in a dose-and time-dependent manner, at least in part, by down-regulating the expression of PCNA. PDTC may be used to prevent and treat the proliferative vitreoretinopathy (PVR).

Objective Discussing the rationality of psychological intervening therapy in the treatment of early breast neoplasm. Methods By summarizing and reviewing 84 patients suffering from the early breast cancer between January of 2003 and December of 2005, we procured some information of their clinical manifes-tations, clinical therapy and statistic results of psychological assessment. Results By intervening the psycho-logical therapy, the patients' self-efficiency and living quality can be bettered. Conclusion Patients with early breast neoplasm need to have a comprehensive treatment with general or local therapy and individual psychologi-cal intervention. Nevertheless, it is rather practical to enhance the general level of prevention and therapy of breast neoplasm through the research of psychological problems after the operation.

Advanced hospitals and high-level hospitals are different from grass-root hospitals,since they not only treat difficult diseases but also have the obligation of scientific studies and teaching.Grass-root hospitals mainly focus on treating common diseases,and only conduct a small amount of teaching,thus resulting in the difference between grass-root hospitals and large-scale hospitals in the aspect of purchasing medical equipment.Because the money for grass-root hospitals may only be used as designated and some even comes from loan or collecting,we have to pinch pennies and make the best use of everything so as to recover the cost rapidly and bring the equipment into full play.

Objective:To study the effects of hepatitis B virus(HBV)infection in vitro on differentiation of mesenchymal stem cells(MSCs)to liver cells.Methods:MSCs were isolated from human bone marrow by density gradient centrifugation,and expanded by adherent culture.MSCs were cultured under liver-stimulating condition,and different composition of serum was added to the induced medium:Group A:5% fetal bovine serum(FBS);Group B:2.5% FBS+2.5% HBV-containing serum;Group C:2.5% FBS+2.5% serum from healthy volunteers;Group D:the undifferentiated MSCs cultured in LG-DMEM+10% FBS.The expressions of a variety of hepatic lineage markers were analyzed by immunocytochemistry and immunofluorescence.The functionality of differentiated cells was assessed by their ablility to store glycogen.After 2 weeks of exposure to HBV infected serum,HBV-specific protein was also detected by immunocytochemistry.Results:As a result of our hepatic induction,the expressions of albumin(ALB)and alpha-fetoprotein(AFP)by MSCs were observed by immunocytochemical and immunofluorescence techniques.Moreover,MSCs had acquired the ability of glycogen storage which was characteristic of liver cells.Compared with the control group,the proliferation of MSCs was inhibited greatly by the virus-containing serum.After 2 weeks of exposure to HBV infected serum,the surface antigen(HBsAg)was detected in some induced MSCs.However,after immunocytochemical stain for ALB and AFP,there was not much difference between the Group B and C.The ability of glycogen storage of two groups were almost the same.Using confocal microscopy,we found the co-expressions of ALB and HBsAg in the same differentiated cells.Conclusion:The bone marrow MSCs have the ability to trans-differentiate into functional hepatocyte-like cells,hence may serve as a cell source for tissue engineering and cell therapy of hepatic diseases.HBV infected serum could inhibit the proliferation of MSCs in culture,but it seemed that the hepatic differentiation of the cell was unsuppressed.

There nearly have 1 year for the medical postgraduate exempted from admission examination to enjoy the postgraduate life,meanwhile to finish the clinicalpractice task as a medical undergraduate.For students,supervisors and the university,analysis of opportunities and difficulties faced by the medical postgraduate exempted from admission examination in this stage can be made,feasible training plan can be developed,in advance to allow students to avoid the psychological confusion,change the way of learning,lay the foundation for scientific research and improve their clinical skills.These measures will contribute to improve the quality of the medical postgraduate exempted fmm admission examination and the education quality.