Objective To investigate the utilization of antibacterials in perioperation for obstetrics and gynecology after the issuing of the notice by the general office of the Ministry of Health "further strengthen in the management of clinical application of antibacterials". Methods According to the survey on the use of antibacterials, 272 cases of surgical patients from April to December 2007 (treatment group) and 270 cases ofsurgical patients from April to December 2008 (control group) in obstetrics and gynecology department were selected,the utilization after the application of antibacterials were investigated and compared. Results The utilization rate of prophylactic use of antibacterials in treatment group and control group were 83.09% (226/272), 84.44%(228/270) respectively (P>0.05). Preoperative use were 77.94%(212/272), 85.19% (230/270) respectively (P< 0.05). The time of medication were (5.8 ± 2.8) d and (4.5 ± 3.8) d respectively (P<0.0l). Drug sensitivity rates were 2.94%(8/272), 2.22%(6/270) respectively (P>0.05). Both used intravenous as the main administration route, double therapy used as the major drug combination. Conclusions Comparison of utilization of antibacterials in perioperation for obstetrics and gynecology before and after the enforcement of "the guiding principle of clinical use of antibacterials" and "further strengthen in the management of clinical application of antibacterials" issued by the general office of the Ministry of Health. The utilization of antibacterials exist some defects:remains high starting point for administration;without an additional if operative time exceed three hours;longer administration time and double therapy as the major drug combination. In summary,there still are some deficiencies that needs co-management and improvement