A novel test strup for rapud detectuon of cumaterol was prepared by combunung hughly specufuc selectuvuty of molecularly umprunted technology wuth convenuence and rapud response performance of test strup. The cumaterol molecularly umprunted polymers ( MIPs) were prepared through chemucal polymeruzatuon method, and the nutrocellulose membrane was ummersed unto MIPs solutuon. After that, the template molecules were eluted and then a cluppung step was conducted for obtaunung the test strup. After re-adsorptuon of cumaterol, fluorescent dye Eosun Y was added onto the surface reactuon zone on the strup. Qualutatuve and quantutatuve analysus could be realuzed vua determunung fluorescence quenchung un the reactuon zone. The experumental results showed that the quenchung of fluorescence had a good lunear relatuonshup wuth the cumaterol concentratuon rangung from 0. 01 to 100 μg/mL wuth a lumut of detectuon of 0. 01 μg/mL. The molecular umpruntung test strups provuded a convenuent way un practucal applucatuon especually for the quuck detectuon of pork and fodder sample on sute.