Objective To discuss the efficacy of Baofukang combined with clindamycin injection in treatment of chronic cervicitis patients with high-risk human papilloma virus infection (hr-HPV).Methods Totally 100 patients who had chronic cervicitis with hr-HPV were selected and divided into two groups randomly.The patients in control group (49 cases) were given Baofukang.The patients in observation group (51 cases) were given Baofukang combined with clindamycin injection.The efficacy of Baofukang combined with clindamycin injection in treatment of chronic cervicitis patients with hr-HPV was evaluated by efficacy,HPV negative rate,inflammatory factors before and after treatment and adverse reaction during treatment.Results After treatment,the effective rate of observation group was 92.1％,and the effective rate of control group was 75.5％,the effective rate of observation group was higher than that of control group (P ＜ 0.05).After treatment,the HPV negative rate in observation group was 90.2％,the control group was 59.2％,the HPV negative rate of observation group was higher than that of control group (P ＜ 0.05).Before treatment,there was no statistical significance on hs-CRP,IL-6,and TNF-α between two groups.After treatment,the hs-CRP,IL-6,and TNF-α were decreased in two groups (P ＜ 0.05).The hs-CRP,IL-6,and TNF-α of observation group were lower than those of control group (P ＜ 0.05).There was no statistical significance on adverse reaction between two groups.Conclusion Baofukang combined with clindamycin injection had a good therapeutic effect on chronic cervicitis with hr-HPV.It could inhibit HPV and inflammatory factors well.It was safe and worthy of clinical use.

OBJECTIVE:To establish a method for the detection of volatile constituents from the leaves and fruits of Piper ni-grum. METHODS:HS-SPME-GC-MS was used. The chromatographic conditions:column was HP-5 MS quartz elastic capillaries, carrier gas was high purity helium(99.999%),flow rate was 1.0 mL/min,the inlet temperature was 250 ℃,initial temperature of column was 50 ℃(temperature programmed),split injection with split ratio of 10:1. MS conditions:ionization mode was electron impact ion source,ionization energy was 80 eV,ion source temperature was 230 ℃,quadrupole temperature was 150 ℃,trans-mission line temperature was 280 ℃,electron multiplier voltage was 1588 V,mass scanning range was m/z 30-400. The spectra were retrieved using RTLPEST3. L and NIST08. L,and the relative contents of the volatile constituents were determined by area normalization method. RESULTS:There were 28 volatile constituents in the leaves and 15 in the fruits,respectively accounting for 67.13% and 36.85%. The major volatile constituents of leaves were β-caryophyllene (15.72%),limonene (9.39%),3-carene (9.32%),β-pinene(6.80%),α-terpine(4.98%),etc.,the main volatile constituents of fruits were 1,7,7-trimethyl-2-vinylbicyclo [2.2.1]hept-2-ene(10.45%),espatulenol(8.28%),caryophyllene oxide(4.81%),etc. 5 constituents were owned in both. CON-CLUSIONS:The study basically clears the main volatile constituents from the leaves and fruits of P. nigrum,and verifies existing obvious differences.

Human trophoblast cells were isolated and cultured in vitro in order to investigate possible pathogenesis of intrauterine infection caused by HCMV. Trophoblast cells were obtained by compound enzymes digestion and discontinuous percoll gradient. Cells and purity were identified by using immunocytochemistry assay with anti-CK7, Vim and beta-hCG antibodies. HCMV AD169 strain replication in isolated trophoblast cells and cell apoptosis were detected at different time points post infection (p.i.). The results showed that highly purified trophoblast cells were obtained. Specific virus replication was increased dramatically at the 24th h p.i., and then increased slowly during 48 h and 72 h. Apoptosis rate of trophoblast cells infected with HCMV was (34.68+/-3.14)% at 24th h p.i., while that in control group was (15.32+/-2.34)% (P<0.05). It was suggested that highly purified trophoblast cells can be isolated by the simplified cell purification method. HCMV can infect human trophoblast cells, and be quickly replicated, resulting in the accelerated apoptosis of human trophoblast cells during early time.

This study examined the impacts of intrauterine murine cytomegalovirus (MCMV) infection on the long-term learning and memory of offspring. Sexually matured male and female BALB/C mice without MCMV infection were identified by ELISA and then mated. Seventy pregnant mice were randomly divided into the virus group (n=40) and the control group (n=30), in which the pregnant mice were subjected to placenta inoculation of MCMV suspension (1 μL, 1×106 PFU) or the same amount of cell culture medium, respectively, at gestational age of 12.5 days. Some pregnant mice [virus group (n=20), control group (n=15)] were sacrificed by cervical dislocation at gestational age of 18.5 days, and the head circumference and brain weight of the mouse fetuses were measured, and the MCMV infection in their brain tissues was detected by PCR. The other pregnant mice [virus group (n=20), control group (n=15)] delivered naturally, and the learning and memory capability of the offspring at 70-day-old was analyzed by Morris water maze test. The results showed that 28.57% mouse fetuses in the virus group developed viral infection in the brain. Their head circumference and brain weight were significantly reduced as compared with those in the control group (P<0.01). The Morris water maze test revealed that the mouse offspring in the control group found the platform with straight-line trajectories after training. In contrast, the counterparts in the virus group intended to enter the central area, but looked for the platform with a circular trajectory. And the infected mice exhibited prolonged swimming distance and swimming latency (P<0.01). It was concluded that: (1) placenta inoculation of MCMV can cause fetal brain infection and intrauterine development retardation; (2) the offspring of MCMV placenta inoculation mice showed a long-term decline in learning and memory capability.

Objective To study the effects of repeated hypoxic exposures (HEs) on glycolysis, mitochondrial oxi-dative phosphorylation and energy charge in mouse brain. Methods Adult BALB/c mice were repeatedly exposed to hypoxia for 5 times and the standard tolerant time and body temperature were recorded. The activities of PFK, PK and mitochondrial complex Ⅰ in the brain were assayed. Phosphoadenosines and energy charge were measured. Results Repeated HEs prolonged the hypoxic tolerance and reduced the body temperature. The activities of PFK and PK experienced regular changes, with an increase in 1st and 3rd HEs and a decline to control levels in 5th HE. The complex Ⅰ activity continued to decrease during HEs. The energy charge was stable. Conclusion HEs lead to a regular change of glycolysis, a continued inhibition of mitochondrial oxidative phosphorylation, and a main-tained energy charge in the brains of mouse.

Objective To determine the effectiveness and medical cost-effectiveness of clinical rehabilitation for promoting the functional recovery of sub-acute cerebral infarction patients.Methods Totally 247 sub-acute patients with cerebral infarction were randomly divided into a clinical rehabilitation group of 129 and a routine rehabilitation group of 118.The clinical group received a standardized rehabilitation intervention and drug treatment,while the routine rehabilitation group received routine rehabilitation therapy and drug treatment.The Fugl-Meyer assessment (FMA) and the modified Barthel index (MBI) were used to compare the two groups after the treatment and 3 and 6 weeks later.The hospital cost for six weeks was also compared between the 2 groups.Results At 3 and 6 weeks,improvement in the average FMA and MBI scores was observed in both groups but the inter-group differences were not significant.The total hospital cost of the clinical group was,however,significantly less than that of the others.Conclusion Clinical rehabilitation can improve the motor function and ability in the activities of daily living of stroke patients.It also has economic benefits.

In the present paper, adhesive test, morphology observation, classified counting and deformation index calculation of the platelets on seven DLC samples made by different processes were carried out after XPS analysis, respectively. Then the effects of carbon phase components on amounts and deformation index of the platelets adhered to the samples were investigated by using the analysis of T-type correlation degree in the grey system theory. It has been shown from results that the amounts and the deformation index of the platelets adhered to the DLC samples made by plasma source ion implantation-ion beam enhanced deposition (PSII-IBED) are obviously less than those of DLC samples made by plasma CVD. The correlation degrees (negative) between the DLC carbon phase and the amounts as well as the deformation index of platelets are much more than those of the other four carbon phase components; besides, larger correlation degrees (positive) only appear between the deformation index of platelets and the C-H carbon phase or C-O carbon phase. It has been indicated that: (1) the effect of DLC carbon phase on platelets adhesion is much greater than that of the other four carbon phase components, the key to improvement in the hemocompatibility of DLC is to increase the DLC carbon phase content; (2) it is necessary to restrain the form or decrease the C-H carbon phase content and C-O carbon phase content so as to control their promotive action on deformation of the platelets adhered to the surface of DLC; (3) using PSII-IBED process to prepare DLC is helpful to improving the hemocompatibility of DLC. These conclusions are essential for designing and improving the deposition process of DLC.
Biocompatible Materials ; Carbon ; chemistry ; Diamond ; chemistry ; Humans ; Male ; Materials Testing ; Models, Biological ; Platelet Adhesiveness

In this study, the diamond like carbon (DLC) samples made from different processes and processing conditions, were subjected to the test of platelet adhesion. The surface energy parameters such as surface tension, critical surface tension, interface tension, adhesive power, and polar branch and chromatic dispersion branch of surface tension based on determining balanced contact angle of ethanol, water and different ratios of ethanol/water solution on surfaces of the samples were calculated, respectively. Then the effects of these parameters on the amounts and deformation index of the platelets adhering to the samples were assessed by analyzing the T-type correlation degree in the Grey system. The results showed: (1) all degrees of correlation between surface energy parameters and adhesion amounts of platelet are positive, but for deformation index, the correlation degrees are negative except for critical surface tension; (2) the adhesion amounts of platelet increase with the rising polar branch of surface tension, while the deformation index increases with rising chromatic dispersion branch of surface tension; (3) Both adhesion amounts and deformation index of platelet are positively correlated with critical surface tension to a higher degrees; (4) the effects of polar branch of surface tension on adhesion amounts and deformation index of platelet keep pace with the surface tension, interface tension and adhesive power. Thus two important conclusions have been obtained: (1) the adhesive characteristic of platelets to the surface of DLC is closely related with the surface energy of DLC; the hemocompatibility of DLC is decided by the balance between the polarity of DLC surface and the limited humidifying water on the surface; there is a blood compatible range delimited by critical surface tension; (2) adhesion and deformation of platelets on surface of DLC have different energy mechanism: polar surface is advantageous to the adhesion, while the deformation is achieved with the aid of chromatic dispersion action stem from the surface.
Biocompatible Materials ; chemistry ; Carbon ; chemistry ; Diamond ; chemistry ; Humans ; Materials Testing ; Platelet Adhesiveness ; Surface Properties

To clarify the reason causing difference of serum proteins adsorbability on different carbonaceous materials, FT-IR-ATR spectra of human serum albumin (HSA) and human serum fibrinogen(HFG) before and after adsorbing on diamond like carbon film (DLC),diamond film (DF) and graphite were analyzed. It has been shown that there are hydrogen bond because of -NH at the interfaces of HSA-DLC, HFG-DF and HFG-graphite. Based on the results, earlier research conclusion that the adsorbability of HSA on DLC higher than that on DF and graphite, but on DF and graphite the adsorption of HFG takes precedence can be explained rationally.
Adsorption ; Blood Proteins ; metabolism ; Diamond ; chemistry ; Graphite ; chemistry ; Humans ; Hydrogen Bonding ; In Vitro Techniques ; Membranes, Artificial ; Spectroscopy, Near-Infrared

In order to clarify the mechanism of blood coagulation for carbonaceous biomaterials, the plasma rich in platelet was obtaining through the centrifugation of fresh human blood containing anticoagulant. Adhesive tests of platelets to surfaces of DLC, diamond film(DF) and graphite was carried out at 37 degrees C. Then, morphology observation, counting and deformation index calculation of the platelets adhering to surfaces of the three kinds of materials were analyzed by SEM. It has been shown that there is no any platelet on the surface of DLC, but on DF and graphite, a lot of platelets are observed with serious deformation of type III-V. The adhesive amounts of platelet on the surface of graphite are more than those on DF, but deformation index of platelets on the surface of DF is more than that on graphite. Three major conclusions have been obtained through comparative analyses with our previous researches and related literatures: (1) Adhesion, deformation and collection of platelets occurred in succession on material surfaces resulting from protein adsorption are the major mechanism of blood coagulation of carbonaceous materials; (2) Deformation degree of platelets is more important hemocompatibility index than consumption ratio of platelets for carbonaceous materials; (3) The purer the DLC, the better is the hemocompatibility. These conclusions possess important directive function for improving and designing carbonaceous materials used in artificial mechanical heart valves.
Biocompatible Materials ; Blood Platelets ; pathology ; Carbon ; Diamond ; Graphite ; Humans ; In Vitro Techniques ; Materials Testing ; Platelet Adhesiveness ; Surface Properties