Objective To establish the reference interval for CA 72-4 in indirect method.Methods All results for CA72-4 that were stored in our laboratory information system of Zhongshan hospital between Jan.2010 and Dec.2012 were included in this study.Outliers were identified and omitted using Stem-and-Leaf&Box Plots in SPSS statistical software.The treated data was divided into several groups according to gender and age.Nonparametric rank sum test was used to observe the difference between male and female participants and Spearman correlation analysis was used to examine the correlation between CA 72-4 and age.Nonparametric reference intervals for CA 72-4 were estimated statistically in two gender sub-groups.Results After 139 cases excluded, there were 1 548 cases of male (Median 1.7, 0.4 to 18.9) and 773 cases of female ( Median 1.8, 0.2 to 18.9 ).There was a significant difference in serum CA 72-4 between male and female participants.No significant difference was found in serum CA 72-4 among age sub-groups.Indirect reference values for CA 72-4 of male and female were respectively 0 to 8.9 U/ml and 0 to 11.6 U/ml.Conclusion Indirect method to establish biological reference interval is a relatively simple and less expensive method under the high rapid development of the hospital information network .It can be used in the periodical review and establishing the reference intervals where the direct method can not be used.

OBJECTIVETo evaluate the effects of folic acid, vitamin B(6) and B(12) supplementation on plasma homocysteine level and risk of cardiovascular disease.

METHODSThe databases, including Embase, Pubmed, Ovid, Biosis, China National Knowledge Infra-structure (CNKI), Wanfang Data, VIP Database for Chinese Technical Periodical (VIP), Chinese Biomedical Literature Database (CMB), were searched to identify random control trials between February 1994 to February 2014 on the effect of folic acid, vitamin B(6) and B(12) supplementation on plasma homocysteine level and risk of cardiovascular disease. The screening, data extraction and quality assessment were conducted in accordance with the inclusion and exclusion criteria by two reviewers independently. The software Review Manager 5.2 was used. Funnel plots and Egger's regression test were applied to evaluate the publication bias.

RESULTSData from 12 studies including 34 481 patients were analyzed using a fixed-effects model. Funnel plot and Egger's test (P > 0.10) confirmed the absence of publication bias. No statistically significant heterogeneity was detected on testing after excluding the sources of heterogeneity (chi-square test, I < 2 < 50%). Baseline homocysteine levels were similar between the placebo and folic acid, vitamin B(6) and B(12) groups (all P > 0.05). Mean homocysteine levels were significantly lower with folic acid, vitamin B(6) and B(12) therapy compared with placebo during follow-up (all P < 0.05). The pooled relative risks with 95% confidence intervals of outcomes for patients treated with folic acid, vitamin B(6) and B(12) supplementation compared with placebo were 0.98 (0.93-1.03) for cardiovascular event, 0.97 (0.87-1.07) for coronary artery disease, 1.00 (0.92-1.08) for myocardial infarction and 0.92 (0.82-1.03) for cardiovascular death.

CONCLUSIONSFolic aicd combined with vitamin B(6) and B(12) treatment significantly reduced plasma homocysteine level, but did not affect the risk of cardiovascular disease. Thus, folic acid combined with vitamin B(6) and B(12) should not be recommended as secondary prevention of cardiovascular diseases.

Asian Continental Ancestry Group ; Cardiovascular Diseases ; China ; Clinical Trials as Topic ; Folic Acid ; Humans ; Myocardial Infarction ; Secondary Prevention ; Vitamin B 12 ; Vitamin B 6 ; Vitamin B Complex