Objective:To prepare monoclonal antibodies with high specificity against different regions of N protein and to analyze antigenic epitopes of SARS-CoV N protein.Methods: Balb/ c mice were immunized with purified different region N protein to prepare McAb by hybridoma technique and select by indirect ELISA.Both of these McAbs were characterized for their antibody titre in the culture supernatant as well as ascites,class,subclass and affinity analysis.The antigen specificity for McAb were evaluated and confirmed by Western blot.The binding site and capability of McAb were observed by ELISA additive assay.Results: Seven hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N1 and two hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N2 were obtained.About the immunoglobulin subclass of McAb,six were IgG1,two were IgG2b and one was IgG3.Western blot showed that the McAbs reacted specifically with SARS-CoV N protein.ELISA additive assay indicated that two antibodies against SARS-CoV N1 recognized same epitopes,while others recognized distinct epitopes.Conclusion: We successfully obtained nine specific McAbs against the different region SARS-CoV N protein after expression and purification of the different part SARS-CoV N protein.It shows that the N1 McAb recognizs the N-terminus of the N protein whereas the N2 McAb recognizs the C-terminus.

Objective to analyze the major problems existing in laboratory medicine and put forward the improvement measures. Methods The dissertations of 69 graduates in Laboratory medicine major in 2008 were analyzed. Results A total of 62 teachers guided 69 students to complete their thesis,and the dissertation score was normal with outstanding dissertations accounting for 4.38% and good dissertations accounting for 65.21%;and medium accounting for 30.43%. Conclusion: The quality of graduation thesis should be promoted from the start of each aspect of the thesis,and quality control should be implemented in the whole process.

Objective To study the application effect of PDCA management mode in the teaching process of practice nurses in spine surgery to reduce the occurrence of nursing errors. Methods A total of 35 practice nurses in spine surgery from March 2014 to March 2015 received the routine teaching mode were set as control group, and 35 practice nurses from April 2015 to April 2016 implemented PDCA management mode were set as intervention group. The occurrence rate of nursing adverse events in two years was observed, and the interns for clinical teaching evaluation model of quality and satisfaction and the intern examination results were summarized. Results The incidence of nursing adverse events was 22.86% (8/35) in control group, and 2.86% (1/35) in intervention group, the difference was statistically significant (χ2= 17.85, P = 0.00). The intern teacher for clinical teaching content arrangement, teaching attitude, detail guidance and skills training indicators evaluation score were (15.46±1.03), (14.18±1.06), (14.61±1.11), (13.30±1.07) points in control group, and (18.26±1.00), (18.48±0.51), (19.43±0.21), (19.16± 0.28) points in intervention group, the differences were statistically significant (t = 11.54- 31.34, P <0.01). The interns given theory and skills examination results were (80.15±1.61), (85.16±1.02) points in control group, and (93.10±2.01), (91.06±2.03) points in intervention group, the difference was statistically significant (t = 29.75, 15.36, all P = 0.00). The clinical teaching satisfaction was 57.14% (20/35) in control group, and 80.00% (28/35) in intervention group, the difference was statistically significant (χ2=12.12, P=0.00). Conclusions PDCA management model applied to clinical practice in department of orthopedics teaching process, can effectively improve the intern theory knowledge and skills to master the ability, most of the interns examination results are more optimistic, and for this kind of management pattern for the process of teaching evaluation of higher satisfaction, the teachers care under the guidance of intern nursing mistake, nursing adverse events is low, this method is worthy of promotion.

Objective To observe the effect of sinomenine on the proliferation of fibroblast-like synoviocytes (FLS) and expression level of MMP-3 secreted by FLS in rheumatoid arthritis in vitro. Methods FLS were obtained by digesting synovial tissues with collagens and were divided into four groups:sinomenine high, middle, low concentration and control group. The proliferation of FLS was assessed by methyl- thiazolyl-tetrazolium (MTT) assay and the mRNA expression of MMP-3 was measured by semi-quantitative RT-PCR respectively after treated by sinomenine. Results The rate of FLS proliferation was significantly reduced in groups treated by sinomenine. Compared with control group, the mRNA expression of MMP-3 markly decreased in sinomenine groups (P

Objective To compare the diagnostic value for hysteromyona with trans-abdominal ultrasound (TAS) and trans-virginal ultrasound(TVS). Methods Two empirical doctors detected respectively 82 patients by TAS and TVS in double blind method. The results were compared with pathological results. Result The correct rate of diagnosis in 82 patients with 123 hysteromyoma focus by TVS and TAS was 96.75% and 86.18% respectively.There was statistical difference between TAS and TVS. The correct rate of combining use of the two method was 98. 37%. Couclusion Either TVS or TAS had its own merit and defect, Combining use of the two method could raise the correct rate.

The estrogen receptor (ER) coregulators regulate the transcriptional activities of ER,and then affect the transcription and translation of target genes mediated by ER.ER coregulators not only plays an important role in the initiation,progression and metastasis of breast cancer,but also involves in the response to endocrine therapy of breast cancer via the genomic and nongenomic function.

ObjectiveTo develop the group intelligence scale for Military Personnel ( Part B),and examining its reliability and validity of the scale.MethodsFollowing the intellectual shock theory,crystal and fluid intelligence theory,the group intelligence Scale for Military Personnel (Part B) had been constructed,which including two subscales,each subscale was divided into two subtests.The data was collected in 1200 military personnel who were chosen by random cluster sampling,then employing SPSS17.0 and AMOS7.0 for item analysis,reliability and validity,analysis of confirmatory factor.Resultsthe subtests' average index of difficulty was 0.55 ( 0.42 ～0.75 ) ; Discrimination index was 0.44 (0.33 ～ 0.52) ; the test-retest reliability between subtests and total scale ranged from 0.796 to 0.891 ; Cronbachs'a coefficient ranged from 0.619 to 0.892; and the split-half reliability ranged from 0.538 to 0.844; exploratory factor analysis revealed that four factors of the scale (including normal knowledge and generalizability,insight and word explain,picture completion and figure inference) could explain 59.067％ of total variance.Furthermore,analysis of confirmatory factor indicated that the four factors model established in the present study fitted the sample data well.ConclusionThe item analysis,reliability and validity of the newly developed group intelligence scale for military personnel (part B) can meet the basic requirements of psychometrics.

Objective To investigate the role of peripheral N-methyl-D-aspartate (NMDA) receptors in the long-term hyperalgesia induced by peripheral injection of formalin in rats. Methods Formalin was injected in the paws of the SD rats with or without the MK-801 pre-injection. OX-42 expressions in the dorsal horn were observed, and the paw withdrawal thermal latency was measured. Results The OX-42 expression decreased and the paw withdrawal thermal latency prolonged in rats with MK-801 pre-injection. Conclusion Peripheral NMDA receptors may be involved in the long-term hyperalgesia.

Pain is classically viewed as being mediated solely by neurons. This view is dramatically changing by new research that spinal cord glial cells amplify pain. Recognition that glial activation is a powerful driving force for exaggerated pain opens up new ways to approach effective clinical pain control. The authors reviewed spinal cord glial cells and cytokines as key players in the creation and maintenance of neuropathic pain.