Objective:To study the action of SH in mid-term cornea preservation.Methods:SH was added into mid-term preservation solution(MMK) to make the new storage media with SH of three different concentration (0.05%,0.03%,0.01%).The contrast group was MMK medium without SH.The corneal appearance,transparence and ultrastructure were observed,and trypan blue-alizarin red staining of CEC,SDH and G-6-P enzyme staining with histochemistry were made in different storage time.Results:The transparence of cornea and ultrastructure of CEC in all the experimental groups were better than that of the contrast.The activity degree of CEC was highest in group I,lowest in the contrast,and statistically significant among all the groups.Conclusion:SH plays an important role in protecting CEC in middle storage medium,and enhances the quality and prolongs the duration of the preserved corneas.The protective effect is determined by the consistence of SH.

Animals ; Cholagogues and Choleretics ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Gardenia ; chemistry ; In Vitro Techniques ; Muscle Contraction ; drug effects ; Muscle, Smooth ; drug effects ; physiology ; Rabbits ; Stomach ; drug effects

Objective To study the dilution model of the early pregnancy test strip for the quantitative detection of serumβ-hu-man chorionic gonadotropin(β-hCG)level by the Abbot AxSYM detection system and its application.Methods The detection re-sults of serumβ-hCG level in 5 612 outpatients and inpatients of the internal medicine department and the gynecology and obstetrics department during 5 years were retrospectively analyzed.In addtion,234 serum samples from the patients were collected for prelimi-narily determining the serumβ-hCG level by the early regnancy test strip and then the quantitative detection of serumβ-hCG level was detected by the Abbot AxSYM detection system.Results The retrospective analysis showed that 241 cases(4.6%)were low-age pregnant women.The qualitative screening was necessary.The screening model was successfully established.But one thing to note was that the part of serum sample needed to be taken out and put into another tube for determination.Conclusion Establishing the rational and effective dilution interpretation model of early pregnancy test strip for screening serumβ-hCG can bring the econom-ic and time benifits for the routine detection work of the Abbot AxSYM detection system.

MicroRNA (miRNA) is newly discovered,a class of small non-coding RNA which plays critical roles in the regulation of gene expression at the posttranscriptional level by either degrading or blocking translation of messenger RNA targets.Growing evidence that some miRNAs have crucial roles in controlling and modulating immunity,while dysregulation of miRNAs can lead to autoimmunity,and miRNA-146 seems to be a popular topic discussed much.Thyroid associated ophthalmopathy (TAO) is an organ specificity autoimmune disease which is closely associated with Graves disease.Although many clinical and experimental investigations have been done,the precise aetiology and pathogenesis of TAO remain largely unclear.Here we review the roles of miRNA-146 regulation in immune functions and in the development of autoimmunity and autoimmune disease,and outlook for the roles of miRNA-146 in the pathogenesis of TAO.

Objective To investigate and analyze the depression situation and the influencing factors in elderly inpatients.Meth-ods The cross-sectional method was used to conduct the investigation on randomly selected 125 elderly inpatients by the geriatric depressive scale(GDS)and the general personal questionnaire.Results Among 125 elderly inpatients,41 cases were depressed eld-erly inpatients,accounting for 32.8%.Disease,marital status,length of hospital stay,self-care ability,medical expenses and hobbies were the influence factors of the elderly inpatients(P<0.05).Conclusion The prevalence of depression in the hospitalized elderly patients is relatively high,the geriatrics should pay attention to it.

Objective To analyze the concentration changes of serum hypoxia stress peptide (HSP)and plasma nitrogen monoxidum(NO)in the entering mountain rapidly man and to explore the importance of HSP and NO in adaptation of man to altitude hypoxia.Methods The levels of serum HSP and plasma NO2-/NO3-were measured from 106 entering mountain rapidly men,105 altitude in habitant,105 altitude immigrant and 104 plain healthy controls,respectively by chromatometry and NO2-/NO3-reagent.Furthermore,the content of serum HSP was determined in 106 entering mountain rapidly men,respectively at the time of 24 h,48 h,76 h,l week and 1 month after entering mountain.Results The level of serum HSP was significantly higher in entering mountain rapidly men than in habitant,immigrant and plain controls(P＜0.05),while there was no statistical difference between in habitant,immigrant and plain controls(P＞0.05).At 24h after entering mountain rapidly,the level of serum HSP was significantly increased and reached the peak.At 48h and 76h,it was slightly decreased,but still maintained a high level.At 1 week and 1 month,it gradually and significantly went down,but was still significantly higher than that in plain controls.Similarly,the level of plasma NO2-/NO3-was significantly elevated in entering mountain rapidly man,compared with inhabitant,immigrant and plain controls(P＜0.05).It was lower in inhabitants and immigrants than plain controls,but the difference was not statistically significant(P＞0.05).Conclusion The present results demonstrated that the levels of serum HSP and plasma NO were significantly up-regulated in healthy adults after en tcring mountain rapidly in order to adapt to altitude stress,and restored gradually to the normal level as time went by.

Animals ; Bile ; physiology ; In Vitro Techniques ; Jejunum ; physiology ; Muscle, Smooth ; physiology ; Rabbits

OBJECTIVE:To establish a method for the contents determination of astragalosidesⅠ,Ⅱ,Ⅲ,Ⅳ,lobetyolin and for-mononetin in Shenqi fuzheng injection. METHODS:HPLC was performed. The detector was evaporative light scattering detector, column was Ultimate XB-C18 with mobile phase of acetonitrile-0.5% formic acid (gradient elution) at a flow rate of 1.2 ml/min, column temperature was 30 ℃,and injection volume was 10 μl. RESULTS:The linear range was 0.62-5.67 μg for astragalosidesⅠ(r=0.999 6),0.78-6.31 μg for astragalosidesⅡ(r=0.999 6),0.36-3.48 μg for astragalosidesⅢ(r=0.999 7),0.81-6.72 μg for as-tragalosides Ⅳ(r=0.999 5),0.82-7.03 μg for lobetyolin(r=0.999 8)and 0.58-6.62 μg(r=0.999 7)formononetin;limit of quan-titation was 1.21,0.15,0.12,0.03,0.12,0.17 μg/ml;limit of detection was 0.35,0.35,0.04,0.01,0.03,0.04 μg/ml;RSDs of pre-cision,stability and reproducibility tests were lower than 2%;recoveries were 95.1%-101.1%(RSD=2.0%,n=9),95.2%-100.7%(RSD=1.9%,n=9),95.8%-100.2%(RSD=1.4%,n=9),96.2%-100.6%(RSD=1.7%,n=9),96.6%-101.2%(RSD=1.4%, n=9) and 95.9%-99.5%(RSD=1.2%,n=9),respectively. CONCLUSIONS:The method is simple and accurate,and can be used for the simultaneous determination of astragalosidesⅠ,Ⅱ,Ⅲ,Ⅳ,lobetyolin and formononetin in Shenqi fuzheng injection.

OBJECTIVE To perform a surveillance study in order to investigate the change in antimicrobial resistance of Klebsiella pneumoniae,especially extended-spectrum ?-lactamases(ESBLs)-producing strains isolated.METHODS A total of 1986 strains of K.pneumoniae isolated during the last six years were analyzed.RESULTS Among 1986 K.pneumoniae strains,908(45.7%) were ESBLs-producing strains.The sensitive rate of ESBLs-producing K.pneumoniae to imipenem was 100%,and to meropenem was 99%.There were 636 strains isolated from ICU,accounted for 32%;and 480 strains isolated from Department of Respiratory Medicine,accounted for 24.2%.There were 1630 strains isolated from sputum,accounted for 82.1%.CONCLUSIONS K.pneumoniae is the major pathogen.Detecting ESBLs-producing strains rate and their susceptibility to antibiotics is very important in guiding the clinical administration of drugs.

Objective To observe the effects of neuromuscular electrical stimulation (NMES) at different frequency on post-stroke pharyngeal dysphagia.Methods Forty-five stroke survivors with pharyngeal dysphagia were randomly divided into treatment group 1,treatment group 2 and treatment group 3,eachof 15 patients.Patients in the treatment group 1,2 and 3 received NMES once,twice and three times a day respectively.All the 3 groups were given conventional swallowing training.The swallowing function classification was conducted before treatment,as well as 1,2 and 4 weeks after the treatment.Before and 2 weeks after the treatment,X-ray examination of swallowing (VFSS) was taken to assess the swallowing function,and the average treating days of the 3 groups were compared.Results The swallowing function classification showed that in treatment group 1 and 2,significant improvement was observed at this time point than that at the previous time point (P＜0.05).In treatment group 3,after 1 and 2 weeks of treatment,significant improvement was found compared to those previously,but there was no difference between after 2 weeks and 4 weeks of treatment.At the same time points,the treatment group 2 and 3 showed higher efficiency than the group 1 (P＜0.05).And compared with the treatment group 2,the treatment group 3 showed higher efficiency after 1 and 2 weeks of treatment (P＜0.05),but there was nosignificant difference after 4 weeks of treatment.Two weeks after the treatment,the average VFSS scores of all groups were significantly higher than those before the treatment (P＜0.05) and that of treatmentgroup 2 (7.600± 1.793) and 3 (8.900± 1.636) was significantly higher than that in treatment group 1 (6.700±1.873),as was that of group 3 compared to group 2.Compared with the treatment group 1 and 2,the average treatment days decreased significantly in treatment group 3 (P＜0.05).Conclusion NMES is effective in treating pharyngeal dysphagia after stroke.And within a certain range (1 to 3 times every day),with the increasing of daily treatment frequency,the effectismore significant and the average time of treatment shortens.