Objective:To prepare monoclonal antibodies with high specificity against different regions of N protein and to analyze antigenic epitopes of SARS-CoV N protein.Methods: Balb/ c mice were immunized with purified different region N protein to prepare McAb by hybridoma technique and select by indirect ELISA.Both of these McAbs were characterized for their antibody titre in the culture supernatant as well as ascites,class,subclass and affinity analysis.The antigen specificity for McAb were evaluated and confirmed by Western blot.The binding site and capability of McAb were observed by ELISA additive assay.Results: Seven hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N1 and two hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N2 were obtained.About the immunoglobulin subclass of McAb,six were IgG1,two were IgG2b and one was IgG3.Western blot showed that the McAbs reacted specifically with SARS-CoV N protein.ELISA additive assay indicated that two antibodies against SARS-CoV N1 recognized same epitopes,while others recognized distinct epitopes.Conclusion: We successfully obtained nine specific McAbs against the different region SARS-CoV N protein after expression and purification of the different part SARS-CoV N protein.It shows that the N1 McAb recognizs the N-terminus of the N protein whereas the N2 McAb recognizs the C-terminus.

Objective to analyze the major problems existing in laboratory medicine and put forward the improvement measures. Methods The dissertations of 69 graduates in Laboratory medicine major in 2008 were analyzed. Results A total of 62 teachers guided 69 students to complete their thesis,and the dissertation score was normal with outstanding dissertations accounting for 4.38% and good dissertations accounting for 65.21%;and medium accounting for 30.43%. Conclusion: The quality of graduation thesis should be promoted from the start of each aspect of the thesis,and quality control should be implemented in the whole process.

Objective To study the application effect of PDCA management mode in the teaching process of practice nurses in spine surgery to reduce the occurrence of nursing errors. Methods A total of 35 practice nurses in spine surgery from March 2014 to March 2015 received the routine teaching mode were set as control group, and 35 practice nurses from April 2015 to April 2016 implemented PDCA management mode were set as intervention group. The occurrence rate of nursing adverse events in two years was observed, and the interns for clinical teaching evaluation model of quality and satisfaction and the intern examination results were summarized. Results The incidence of nursing adverse events was 22.86% (8/35) in control group, and 2.86% (1/35) in intervention group, the difference was statistically significant (χ2= 17.85, P = 0.00). The intern teacher for clinical teaching content arrangement, teaching attitude, detail guidance and skills training indicators evaluation score were (15.46±1.03), (14.18±1.06), (14.61±1.11), (13.30±1.07) points in control group, and (18.26±1.00), (18.48±0.51), (19.43±0.21), (19.16± 0.28) points in intervention group, the differences were statistically significant (t = 11.54- 31.34, P <0.01). The interns given theory and skills examination results were (80.15±1.61), (85.16±1.02) points in control group, and (93.10±2.01), (91.06±2.03) points in intervention group, the difference was statistically significant (t = 29.75, 15.36, all P = 0.00). The clinical teaching satisfaction was 57.14% (20/35) in control group, and 80.00% (28/35) in intervention group, the difference was statistically significant (χ2=12.12, P=0.00). Conclusions PDCA management model applied to clinical practice in department of orthopedics teaching process, can effectively improve the intern theory knowledge and skills to master the ability, most of the interns examination results are more optimistic, and for this kind of management pattern for the process of teaching evaluation of higher satisfaction, the teachers care under the guidance of intern nursing mistake, nursing adverse events is low, this method is worthy of promotion.

Objective To understand the relationship between coagulation function and birth weight and gestational age in newborn. Methods Five hundred premature infants in NICU from June 2015 to June 2016 were enrolled. Perinatal high-risk factors(gestational hypertension, gestational diabetes, placenta previa, spontaneous premature delivery) and general status (gestational age, sex, delivery mode, birth weight)were documented. Blood coagulation function (PT, APTT, FDP, DD, FIB, AT-Ⅲ) was detected within 6 h after birth. The premature infants were divided into three groups according to gestational age, early preterm neonate group (28-30+6 weeks, 48 cases), moderate preterm neonate group (31-33+6 weeks, 125 cases), and late preterm neonate group (34-36+6 weeks, 216 cases). And there were 111 term infants (37- 42 weeks, 111 cases). In addition , the relation between birth weight and coagulation function was analyzed. Sixty-six twins were collected and divided into tow groups according to their weight, the-lighter group and the-heavier group. The coagulation function was analyzed and compared among different gestational age groups. Results There were no differences in perinatal risk factors (P > 0.05). There were significant differences in various coagulation parameters among three groups [APTT: (93.25 ± 21.34), (82.80 ± 21.37), (78.29 ± 17.77), (62.03 ± 13.7) s;FIB: (1.95 ± 0.96), (1.67 ± 0.60), (1.51 ± 2.44), (1.40 ± 0.85) g/L; DD: (2.11 ± 0.64), (1.02 ± 0.33), (0.66 ± 0.31), (0.51 ± 0.25) mg/L;AT-Ⅲ:(25.89 ± 11.80)%, (33.86 ± 11.40)%, (36.65 ± 13.60)%, (45.18 ± 14.82)%] (P<0.05). The levels of APTT, FDP, DD tended to decrease with the increasing of gestational age. The level of FIB and AT-Ⅲtended to increase with the increasing of gestational age. Correlation analysis showed that the levels of APTT, FDP, and DD were negatively correlated with gestational age (P<0.05) and the levels of FIB and AT-Ⅲwere positively correlated with gestational age (P<0.05). In the twins group, no significant difference was showed in different weight (P > 0.05). Conclusions The coagulation function in early life in premature infants is associated with gestational age, and has no relationship with birth weight. The coagulation function of premature infants is in a changing and gradually mature process, which should be monitored dynamically.

Middle database of ACCESS is used to develop non-dispensed drug inquiry system. Current Oracle data structure is not destroyed and the requirements of hospitals can be satisfied.

Objeetive To investigate the clinical effects and nursing interventions of endoscopic sphincterotomy(EST)for treating elderly patients with choledocholithiasis.Methods The clinical data of 73 cases of patients operated by EST were retrospectively analyzed.Before surgery the psychological nursing,examination and preparation were meticulously performed,the changes of vital signs and nursing were closely observed during surgery,the respiratory tract nursing,cardiovascular disease and diabetes nursing,pain nursing and complications treatment were performed after surgery.Results There were 69cases of 73 patients were successful,the success rate was 94.5%;8 cases of concurrent hyperamylasemia were given conservative treatment and had clinical recovery;2 cases of fever occurred and white blood cell count elevated and were treated by effective antibiotic treatment;there was no perforation,wound bleeding and death.Conclusions Using the EST to treat the choledocholithiasis of elderly patients has good clinical effects and low risk;through careful observation and nursing,can reduce complications,promote the patients'early recovery and improve quality of life of elderly patients with choledocholithiasis.

Objective To compare the diagnostic value for hysteromyona with trans-abdominal ultrasound (TAS) and trans-virginal ultrasound(TVS). Methods Two empirical doctors detected respectively 82 patients by TAS and TVS in double blind method. The results were compared with pathological results. Result The correct rate of diagnosis in 82 patients with 123 hysteromyoma focus by TVS and TAS was 96.75% and 86.18% respectively.There was statistical difference between TAS and TVS. The correct rate of combining use of the two method was 98. 37%. Couclusion Either TVS or TAS had its own merit and defect, Combining use of the two method could raise the correct rate.

Objectives: To study the reliability and sensitivity of serum transferrin receptor(sTfR) and sTfR/SF in assessing iron status,and explore the efficacy for monitoring early iron deficiency. Methods:941 women at reproductive age from Hebei Province and Shunyi county were included in the study. We determined the serum ferritin (SF)、zinc protoporphyrin (ZPP) and hemoglobin (Hb), and divided them into four groups: iron normal, IDS (iron deficiency store),IDE (iron deficiency erythropoiesis),IDA (iron deficiency anemia), according to the current criteria for assessing iron status. We randomly selected 189 aliquotes of serum from four groups to determine their sTfR and calculated the ratio of sTfR/SF, ie, sTfR/LogSF、Log (sTfR/SF). Results: sTfR increased significantly according to ID status of different stages,but sTfR/SF greatly increased in all stages. sTfR correlated with the conventional iron status parameters significantly, and could reflect the different aspects of iron status. The efficacy of Log (sTfR/SF) in identifying iron store deficiency reached 99%,higher than sTfR/LogSF and sTfR. sTfR can effectively identify functional iron deficiency,with the efficacy of 83%. Conclusion: sTfR and sTfR/SF all are promising for assessing iron status and screening iron deficiency in women at reproductive age.