Objective:To prepare monoclonal antibodies with high specificity against different regions of N protein and to analyze antigenic epitopes of SARS-CoV N protein.Methods: Balb/ c mice were immunized with purified different region N protein to prepare McAb by hybridoma technique and select by indirect ELISA.Both of these McAbs were characterized for their antibody titre in the culture supernatant as well as ascites,class,subclass and affinity analysis.The antigen specificity for McAb were evaluated and confirmed by Western blot.The binding site and capability of McAb were observed by ELISA additive assay.Results: Seven hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N1 and two hybridoma cell lines secreting monoclonal antibodies against SARS-CoV N2 were obtained.About the immunoglobulin subclass of McAb,six were IgG1,two were IgG2b and one was IgG3.Western blot showed that the McAbs reacted specifically with SARS-CoV N protein.ELISA additive assay indicated that two antibodies against SARS-CoV N1 recognized same epitopes,while others recognized distinct epitopes.Conclusion: We successfully obtained nine specific McAbs against the different region SARS-CoV N protein after expression and purification of the different part SARS-CoV N protein.It shows that the N1 McAb recognizs the N-terminus of the N protein whereas the N2 McAb recognizs the C-terminus.

Objective to analyze the major problems existing in laboratory medicine and put forward the improvement measures. Methods The dissertations of 69 graduates in Laboratory medicine major in 2008 were analyzed. Results A total of 62 teachers guided 69 students to complete their thesis,and the dissertation score was normal with outstanding dissertations accounting for 4.38% and good dissertations accounting for 65.21%;and medium accounting for 30.43%. Conclusion: The quality of graduation thesis should be promoted from the start of each aspect of the thesis,and quality control should be implemented in the whole process.

Objective To study the application effect of PDCA management mode in the teaching process of practice nurses in spine surgery to reduce the occurrence of nursing errors. Methods A total of 35 practice nurses in spine surgery from March 2014 to March 2015 received the routine teaching mode were set as control group, and 35 practice nurses from April 2015 to April 2016 implemented PDCA management mode were set as intervention group. The occurrence rate of nursing adverse events in two years was observed, and the interns for clinical teaching evaluation model of quality and satisfaction and the intern examination results were summarized. Results The incidence of nursing adverse events was 22.86% (8/35) in control group, and 2.86% (1/35) in intervention group, the difference was statistically significant (χ2= 17.85, P = 0.00). The intern teacher for clinical teaching content arrangement, teaching attitude, detail guidance and skills training indicators evaluation score were (15.46±1.03), (14.18±1.06), (14.61±1.11), (13.30±1.07) points in control group, and (18.26±1.00), (18.48±0.51), (19.43±0.21), (19.16± 0.28) points in intervention group, the differences were statistically significant (t = 11.54- 31.34, P <0.01). The interns given theory and skills examination results were (80.15±1.61), (85.16±1.02) points in control group, and (93.10±2.01), (91.06±2.03) points in intervention group, the difference was statistically significant (t = 29.75, 15.36, all P = 0.00). The clinical teaching satisfaction was 57.14% (20/35) in control group, and 80.00% (28/35) in intervention group, the difference was statistically significant (χ2=12.12, P=0.00). Conclusions PDCA management model applied to clinical practice in department of orthopedics teaching process, can effectively improve the intern theory knowledge and skills to master the ability, most of the interns examination results are more optimistic, and for this kind of management pattern for the process of teaching evaluation of higher satisfaction, the teachers care under the guidance of intern nursing mistake, nursing adverse events is low, this method is worthy of promotion.

Objective To explore the usefulness of enhanced patient education in increasing the patients' knowledge and awareness of peripherally inserted central catheter (PICC) protection.Methods Totally 200 patients with PICC in Neijiang First People's Hospital from June 2009 to June 2012 were divided into control group (odd numbers,conventional patient education,n =100) and observation group (even numbers,enhanced patient education,n =100) according to their admission numbers.The patients' knowledge and awareness of PICC protection were analyzed after treatment,and the incidences of adverse events and catheter indwelling time were also compared between these two groups.Results Compared with the control group,the observation group had significantly higher PICC protection knowledge rate (91％ vs.78％,P =0.001),awarehess rate (95％ vs.80％,P=0.011),lower incidence of adverse events (1％ vs.5％,P =0.034),and longer PICC indwelling time [(125.8 ± 10.6) d vs.(106.7 ±8.2) d,P =0.026].Conclusion Enhanced patient education can effectively enhance the patients' knowledge and awareness of PICC protection.

ObjectiveTo develop the group intelligence scale for Military Personnel ( Part B),and examining its reliability and validity of the scale.MethodsFollowing the intellectual shock theory,crystal and fluid intelligence theory,the group intelligence Scale for Military Personnel (Part B) had been constructed,which including two subscales,each subscale was divided into two subtests.The data was collected in 1200 military personnel who were chosen by random cluster sampling,then employing SPSS17.0 and AMOS7.0 for item analysis,reliability and validity,analysis of confirmatory factor.Resultsthe subtests' average index of difficulty was 0.55 ( 0.42 ～0.75 ) ; Discrimination index was 0.44 (0.33 ～ 0.52) ; the test-retest reliability between subtests and total scale ranged from 0.796 to 0.891 ; Cronbachs'a coefficient ranged from 0.619 to 0.892; and the split-half reliability ranged from 0.538 to 0.844; exploratory factor analysis revealed that four factors of the scale (including normal knowledge and generalizability,insight and word explain,picture completion and figure inference) could explain 59.067％ of total variance.Furthermore,analysis of confirmatory factor indicated that the four factors model established in the present study fitted the sample data well.ConclusionThe item analysis,reliability and validity of the newly developed group intelligence scale for military personnel (part B) can meet the basic requirements of psychometrics.

Atherosclerosis is a multifactorial disease and mediated by chronic inflammation from initiation to progression. Inflammatory factors of this process, such as C-reactive protein, interleukin-6, cell adhesion molecules and tumor necrosis factor-a, not only involve in atherosclerosis-related disease process, but also indicate the risk of cardiovascular events. Inflammatory factors level plays a significant role in diagnosis, treatment and prognosis of atherosclerosis-related disease, such as acute coronary syndrome. Recent studies have shown that there is a marked inflammatory response after stenting, which plays an important role in the vascular remodeling process, especially in-stent restenosis. Therefore, it is necessary to explore the molecular mechanism and pathological changes in the process of inflammation and to study prevention and treatment of the inflammation. We believe that inflammatory processes will be new targets of therapy in preventing or treating atherosclerosis and its complications.

Objectives: To study the reliability and sensitivity of serum transferrin receptor(sTfR) and sTfR/SF in assessing iron status,and explore the efficacy for monitoring early iron deficiency. Methods:941 women at reproductive age from Hebei Province and Shunyi county were included in the study. We determined the serum ferritin (SF)、zinc protoporphyrin (ZPP) and hemoglobin (Hb), and divided them into four groups: iron normal, IDS (iron deficiency store),IDE (iron deficiency erythropoiesis),IDA (iron deficiency anemia), according to the current criteria for assessing iron status. We randomly selected 189 aliquotes of serum from four groups to determine their sTfR and calculated the ratio of sTfR/SF, ie, sTfR/LogSF、Log (sTfR/SF). Results: sTfR increased significantly according to ID status of different stages,but sTfR/SF greatly increased in all stages. sTfR correlated with the conventional iron status parameters significantly, and could reflect the different aspects of iron status. The efficacy of Log (sTfR/SF) in identifying iron store deficiency reached 99%,higher than sTfR/LogSF and sTfR. sTfR can effectively identify functional iron deficiency,with the efficacy of 83%. Conclusion: sTfR and sTfR/SF all are promising for assessing iron status and screening iron deficiency in women at reproductive age.

Objective:To explore a simple way to improve seeding efficiency and uniformity in constructing 3-D dermal equivalent,in order to effectively construct tissue engineered skin.Methods: Human dermal fibroblasts were added onto collagen sponge scaffolds and cultured in the following 3 ways:(1)oscillating seeding and oscillating culture(OO),(2)oscillating seeding and static culture(OS),and(3)static seeding and static culture (SS).Samples were obtained and subjected to light microscopy,scanning electron microscopy(SEM) and quantitative cell number assay at planed times.Results: Oscillating seeding achieved more uniform spatial cell distribution within scaffolds as compared to static seeding.Moreover,fibroblast seeding efficiency was significantly higher in oscillating conditions(\%) than in static conditions(\%,P

0.05). But after 12 weeks, blood glucose level and urine volume in DM group were higher and greater than those in control group (both P0.05). (2) The urine osmolality in DM group was decreased while the plasma osmolality increased (P