Humans ; Polymorphism, Genetic ; Pulmonary Fibrosis ; genetics ; physiopathology ; Transforming Growth Factor beta ; genetics ; physiology

OBJECTIVE: To evaluate the effects of fluvastatin on insulin resistance and hs-CRP level in metabolic syndrome rat model. METHODS: Sixty male KM rats were randomly divided into two groups: 10 rats in control (normal diet) and 50 rats in model (high lipid diet) group. By the end of the 4th week, 10 rats selected randomly from the model group were killed to verify the establishment of metabolic syndrome model. Since the 5th week, the remainder 40 rats were randomly divided into A, B, C and D groups, then were administered intragastrically with fluvastatin capsules at 6 mg · kg-1 · d-1, fluvastatin capsules at 12 mg · kg-1 · d-1, fluvastatin slow-release tablets at 12 mg · kg-1 · d-1 and saline, respectively, and all the rats were fed on normal diet for 4 weeks. All the rats were killed by the end of the 8th week. The levels of TC, TG, LDL-C, HDL-C, FBG, FINS, and hs-CRP were measured respectively and then homeostasis model assessment of insulin resistance was calculated. RESULTS: By the end of the 4th week, the metabolic syndromes model was established. At the end of the experiment, compared with group D, the levels of TC, TG and LDL-C were decreased significantly(P < 0.01) while HDL-C were increased significantly in A, B and C groups(P < 0.05); the levels of hs-CRP, FBG and FINS were decreased significantly(P < 0.01). Compared with group A, the levels of TC, TG and LDL-C were decreased significantly (P < 0.05), while the levels of hs-CRP, FBG and FINS had no change in group B(P > 0.05); compared with group B, the levels of TC, TG, LDL-C, hs-CRP, FBG, FINS and HOMA-IR were decreased significantly in group C (P < 0.05). CONCLUSION: Fluvastatin can reduce TC, TG, LDL-C, FINS, and hs-CRP levels and improve insulin resistance, the effects is more apparent at a large-dosage and with slow-release tablets especially, and that may attribute to its anti-inflammation effects.

AIM:To compare the clinical efficacy of three combined surgical treatments for cataract induced by silicone oil -filled eye.
METHODS: The data of 75 eyes of 75 patients with cataract induced by silicone oil-filled eye were reviewed. Those patients were divided into group A, B, C. The patients in group A were treated by phacoemulsification combined with silicone oil removal through pupil and IOL implantation, the patients in group B were treated by phacoemulsification combined with silicone oil removal through 23G vitrectomy system and IOL implantation, the patients in group C were treated by phacoemulsification combined with silicone oil removal through 20G vitrectomy system and IOL implantation. The operation time, intraocular pressure, best corrected visual acuity ( BCVA ) , discomfort days and complications before and after the surgery were compared.
RESULTS: The differences of the intraocular pressure were not statistically significant between the three groups after the surgery. The BCVA of the three groups were all improved, but the differences were not statistically significant between three groups. The differences in postoperative complications showed no statistically significant. Otherwise, group A and B cost less operation time. The discomfort days of group A were less than other groups.
CONCLUSION: Three different surgical methods were safe and effective, appropriate surgical approach could be chosen according to the patients' clinical manifestations. For patients with cataract induced by silicone oil-filled eye whose retina recovered well, phacoemulsification combined with silicone oil removal through pupil and IOL implantation is a time-saving, safe and effective method.

Objective:To investigate the effect of homocysteine (Hcy) on peripheral vascular plaque formation in patients with type 2 diabetes mellitus (T2DM) combine subclinical hypothyroidism (SCH), and to provide guidance for clinical medication and prognosis judgement.Methods:A total of 125 patients with type 2 diabetes admitted to the NO.3201 Hospital from 2018 Jan to Dec 2019 were selected. 125 patients with type 2 diabetes mellitus were divided into T2DM without plaque group (40 cases), plaque group (23 cases), T2DM with SCH without plaque group (25 cases) and plaque group (37 cases) according to thyroid function and whether they had peripheral vascular plaque. Data were collected to analyze the influencing factors of peripheral vascular disease.Results:(1) The incidence of vascular plaque in T2DM group and T2DM with SCH group was 36.5%(23/63) and 59.7%(37/62), respectively, with significant difference ( P<0.05). (2) There were significant differences in serum thyroid stimulating hormone (TSH), apolipoprotein A (ApoA), triglyceride (TG) and Hcy between T2DM group and T2DM with SCH group ( P<0.05); there was significant difference in low-density lipoprotein (LDL-C) and Hcy between T2DM groups with or without plaque ( P<0.05); there were significant differences in ApoA and Hcy between T2DM with SCH group with or without plaque ( P<0.05). (3) Logistic regression analysis showed that Hcy was the risk factors for the occurrence of vascular plaque in T2DM and T2DM with SCH group ( OR=1.640, 2.695, P<0.05). (4) Receiver operating characteristic (ROC) curve showed that the area under the curve of Hcy in T2DM group was 0.842 and Youden index was 15.75 μmol/L; The Hcy's area under ROC curve was 0.945 and Youden index was 12.9 μmol/L in T2DM with SCH. Conclusions:Hcy is closely related to the presence of peripheral vascular plaque in T2DM patients with subclinical hypothyroidism. The detection of blood Hcy level can provide new ideas for early diagnosis and treatment of peripheral vascular lesions in T2DM patients with SCH.

Objective To investigate the carriage of nuc-mecA gene among different altitudes in high humidity district,provid-ed guiding data for prevention of staphylococcus aureus and drug-resistant bacteria,standardizing the usage for antibiotics. Methods The nose swabs were collected in different altitudes:1 000 m,1 200 m and 1 400 m,nuc-mecAgene was confirmed by multi-channel real-time PCR.Results The carrier of nuc gene in the noses were 4.878%,2.899% and 7.143%,in 1 000 m,1 200 m and 1 400 m respectively,and there were no statistical significant among the altitudes (P>0.05).The carrier of mecA gene were 14.634%,31.884% and 41.837% in the 1 000 m,1 200 m and 1 400 m respectively,the difference showed statistical significe (P<0.05).The carrying rate of mecA gene showed statistically significant differences between 1 000 m and 1 200 m (P<0.05),and showed statistically significant differences between 1 000 m and 1 400 m (P<0.01)also.The carrier of nuc-mecA gene were 0%,1.449% and 3.061% in 1 000 m,1 200 m and 1 400 m respectively,there were no statis-tical significant (P>0.05).Conclusion The carrier of mecA gene in noses was increased with the increasing of the altitude. The residents who living at higher altitude should keep the colonization sites of pathogens clean,and needed timely medical when got sick,shouldn’t abuse the antibiotics without authorization.Medical staff should rational use of antibiotic drugs,a-voided overusing of antibiotics and overtreatment.

Objective:To analyse the salivary cortisol changes in patients with temporomandibular disorders(TMD)after electrical stimulation,and the relationship between cortisol and pain catastrophizing.Methods:1 1 TMD and 1 1 healthy subjects were evoked by electrical stimulation.The saliva samples were collested before test,50 s and 1 00 s of stimulation after test seperately.Salivary cortisol was analysed by ELISA kit.The pain catastrophizing scale(PCS)was answered by all the subjects before stimulation.Data were statis-tically analysed.Results:The salivary cortisol concentration in TMD patients was higher than that in the controls(P <0.05).After stimulation,it showed a downward trend.No interaction between time and groups.The total scores of PCS including three indexes(ru-mination,magnification,helpless)were positively correlated with salivary cortisol concentration.Conclusion:The unpredictable stress causes high level of cortisol.which is related to pain catastrophizing.

Objective To investigate the difference of serum thymidine kinase 1(TK1) levels of young and middle-aged people in high humidity and high altitude area .Methods Serum level of TK1 was measured with the enhanced chemiluminescence method in 128 native young and middle-aged people .Results Serum TK1 was (1 .784 2 ± 0 .127 9)pmol/L in the control group and (1 .385 0 ± 0 .078 5)pmol/L in the test group ,the difference between them had statistical significance (P<0 .01) .28 cases in the control group were TK1 positive with the positive rate of 30 .43% ,while 2 cases in the test group were TK1 positive with the posi-tive rate of 5 .56% ,the positive rates had statistical difference between the two groups (P<0 .01) .In the control group ,the TK1 level in females was higher than that in males ,the difference between them showed statistical significance (P<0 .05) .Conclusion Detecting serum TK1 in the population of high humidity and high altitude areas has the active significance for guiding the physical examination and cancer prevention of different age groups under the special environment .

Objective To investigate the carrying situation of nuc-mec A gene for different departments in the inpatient depart-ment and outpatient department to provide the data for the hospital infection control and reaching the target for preventing nosoco-mial infection and improving the cure rate.Methods The research subjects were divided into 4 groups:osteoarthropathy and hemi-plegia rehabilitation department group(A,44 cases),comprehensive surgical and hematology-oncology department group(B,32 ca-ses),cardiopulmonary department group(C,17 cases)and outpatient department group(D,35 cases).The group A,B and C were the persons in the inpatient department and the group D were the persons in the outpatient department,aged 30-84 years old.Na-sal swab samples were collected and the nuc-mec A gene was detected by the multi-channel real-time PCR.Results No person in the group A,B and C carried nuc gene and 1 case(2.86%)in the group D carried nuc gene.There were no statistically significant differ-ence among 4 groups(P >0.05);the carriers of mecA gene in the group A,B,C and D were 26,17,13 and 10 cases,the carrying rates were 59.10%,53.10%,76.50% and 28.60% respectively,the difference showed statistical significance(P <0.01),but which in the group A,B and C had no statistically significant difference(P >0.05);the carrying rate of nuc-mec A gene showed statistically significant differences between the patients and the medical staff(P <0.05),but which had no statistically significant differences be-tween the patients and the medical staff in each group(P >0.05).Conclusion nuc-mec A gene generally exists in the inpatient de-partment and the carrying rate of nuc-mec A gene is relatively lower,which indicates that the inpatient department needs to pay at-tention to the prevention and treatment of pathogenic bacteria,especially the de-clonization in the hemiplegia rehabilitation and car-diopulmonary departments for avoiding cross-infection among different departments.

Objective:To observe the effect of two different apical surgery timing. Methods:68 patients with periapical lesion were divided into 2 groups. 30 patients( control group) were operated by apical surgery at least 1-2 months after root canal therapy( RCT) , while 38 patients(experimental group) were operated immediately after RCT. The patients were followed up 3, 6 and 12 months after apical surgery. Results:The curative effect analysed with the age, sex and tooth position showed no statistical difference between 2 groups(P>0. 05). Conclusion:Immediate and delayed apical surgery apical surger after RCT are similarly effective.

Objective: To observe the therapeutic efficacy and safety of sequential administration of erlotinib following chemotherapy in patients with erlotinib (as second-line treatment)-resistant advanced NSCLC (non-small cell lung cancer). Methods: Thirty stages ?B-? NSCLC patients with gradual progression after erlotinib treatment as second-line therapy were retrospectively reviewed. Of the 30 patinets, 15 patinets (group A) received one cycle of chemotherapy (pemetrexed plus cisplatin) followed by erlotinib, the remaining 15 patinets (group B) received chemotherapy alone (pemetrexed plus cisplatin). The short-term response and side effects were observed and the survival was analyzed. Results: The objective response rates of group A and group B were 13.3% (2/15) and 6.7% (1/15), respectively (P = 1.000), and the disease control rates were 40.0% (6/15) and 20.0% (3/15), respectively (P = 0.426). The median progression-free survival of group A and group B were 6.0 and 4.0 months, respectively (P = 0.001). There were no significant differences in the rates of side effects between the two groups. Conclusion: The administration of erlotinib following chemotherapy may offer benefits to the patients with erlotinib (as second-line treatment)-resistant advanced NSCLC. This treatment is also generally tolerable. Copyright © 2013 by TUMOR.