Objective To study the expression ofinterleukin 10(IL-10) and interleukin 17(IL-17)in patients with acute gastritis,and analyze the clinical significance.Methods The expression of IL-10 and IL-17 in the serum of 58 patients with acute gastritis (gastritis group) and 40 healthy cases (control group) was detected by ELISA.The patients in gastritis group included 23 patients of slight degree,18 patients of middle degree and 17 patients of severe degree.Results The level of IL-10 in gastritis group [(108.321 ±12.302) ng/L] was significantly higher than that in control group [(17.883 ± 13.802) ng/L] (t =18.143,P ＜ 0.01).The level of IL-17 in gastritis group [(127.418 ± 11.410) ng/L] was significantly higher than that in control group [(30.335 ± 14.549) ng/L] (t =22.493,P ＜ 0.01).The level of IL-10 and IL-17 in slight degree,middle degree and severe degree patients was significantly increased [IL-10:(33.486 ± 14.568)ng/L,(106.189 ± 13.583) and (123.875 ± 14.892) ng/L;IL-17:(68.209 ± 9.332),(129.856 ± 11.456),(208.165 ± 12.854) ng/L](P＜ 0.05).The level of IL-10 had positive correlation with IL-17 (r =0.79,P＜0.01).Conclusions IL-10 and IL-17 participate in the pathogenesis of acute gastritis and they have high correlation.IL-10 may restrain IL-17 cells and control the progress of inflammation.

AIM:To study the effect of antioxidation in subacute aging model mice with Jiangtang compound recipe which composed of kudzuvine root, heartleaf houttuynia herb and Evonymus alatas. METHODS: By using the D-galactose-induced aging mice, the body weight,the content of malondialdehyde (MDA),the activities of superoxide dismutarse(SOD) and glutothine peroxidase(GSH-Px) in serum and tissues were determined. RESULTS:To the model mice, Jiangtang compound recipe (14, 7 g/kg, i.g.) reduced the content of MDA, enhanced the activities of SOD and GSH-Px in serum and tissuses, inhibited the decrease of body weight. CONCLUSION: Jiangtang compound recipe shows a remarkable anti-oxidative effect on the D-galactose-induced aging mice.

Objective To investigate clinical characteristics and outcome of patients with liver cirrhosis complicated with diabetes,and to explore the differences of clinical characteristics and prognosis between hepatogenous diabetes (HD) and type 2 diabetes mellitus (T2DM).Methods From November 2010 to April 2012,246 patients with liver cirrhosis were collected and divided into liver cirrhosis complicated with diabetes (LC-DM) group (n=72) and liver cirrhosis without diabetes (LC) group (n=174).LC-DM group was then sub-divided into HD group (n=33) and T2DM group (n =39).All the patients were followed up until death or the study endpoint.The clinical characteristics,prognosis and indicators of glucose metabolism were compared and analyzed.Oral glucose tolerance test and insulin C-peptide release test were conducted in part of patients in LC-DM group.Student t test was performed for mean comparison between two groups.Chi-square test was used for count data and rate comparison between two groups.The death related factors of liver cirrhosis were analyzed by Logistic regression analysis.Results The percentage of alcoholic liver cirrhosis of LC-DM group was 18.1 ％ (13/72),which was higher than that of LC group (6.9％,12/ 174) and the difference was statistically significant (x2=6.946,P=0.008).In HD group,the percentage of patients with diabetes family history was 9.1％ (3/33),which was lower than that of T2DM group (30.8％,12/39),the percentage of alcoholic liver cirrhosis was 30.3％ (10/33),which was higher than that of T2DM group (7.7％,3/39),the total bilirubin level ((43.4±57.7) μmol/L) was higher than that of T2DM group ((22.6 ± 13.3) μmol/L),the total cholesterol level ((3.3 ±1.2) mmol/L) was lower than that of T2DM group ((4.0±1.6) mmol/L),and the differences were statistically significant (x2=5.093 and 6.177,t=2.178 and 2.014,P=0.024,0.013,0.033 and 0.048).The duration of hospitalization,Chiid-Pugh score,the incidence of upper gastrointestinal bleeding,the incidence of hepatic encephalopathy,electrolyte disturbances and mortality of LC-DM group were all higher than those of LC group and the differences were statistically significant(t=2.389 and 2.270,x2 =6.496,5.572,5.194,19.646,all P＜0.05),and there was no significant difference between HD group and T2DM group(all P＞0.05).The blood glucose levels of HD group at different time point (0,30,60,120,180 min) were all lower than those of T2DM group and the differences were statistically significant (t=3.175,2.345,3.444,3.090,3.542,all P＜0.05).The fasting insulin level of HD group was close to that of T2DM group,the insulin levels of HD group at other time point (30,60,120,180 min) were all higher than those of T2DM group and the differences were statistically significant (t =3.084,2.881,3.648,2.840,all P＜ 0.05).There was obvious hyperinsulinemia in HD group.At 0,30,60,120,180 min,C-peptide level of HD group was at normal levels,and which of T2DM group was lower than normal.Child-Pugh grade C was an independent predictor factor of death in patients with liver cirrhosis (OR =3.056,95 ％ CI:1.268 ～ 7.346,P=0.013).Conclusions Compared with liver cirrhosis patients without diabetes,the liver function of patients complicated with diabetes was worse,and the incidence of complication and mortality was higher.However there was no significant difference between HD group and T2DM group.

Juvenile idiopathic arthritis (JIA) is the most common chronic arthropathy in childhood. Immune and genetic factors take part in the development of JIA and influence its consequence. Studies have identified association between JIA and genes encoding the human leukocyte antigens (HLA) . In this article we discuss the principles behind genetic studies of complex traits like JIA, and comprehensively catalog HLA candidate gene associated with JIA to date, and review several validated associations. Studies indicate that the HLA-Ⅰ, Ⅱ gene and their products play an important role in JIA pathogenesis, clinical course, hormonal effect and prognostic judgment. Studies in different ethnic groups indicate the main relationship between JIA and HLA is concentrated upon A, DR and DQ loci, especially the HLA-Ⅱ gene and some haplotypes. The different type of JIA is always with different HLA susceptible locus and protective locus. Different race, different region and different social environment also affect the JIA's susceptible gene polymorphism. Finally we discuss the problems existed in the study of JIA susceptibility and HLA polymorphism.

Objective To observe the effect of Xuebijing injection on organs injury in patients with acute paraquat poisoning.Methods Thirty patients with paraquat poisoning were randomly divided into two groups.The improvement of liver and kidney function,SaO2,rate of death were observed and compared between the two groups.Results The ABG,SaO2,GGT and Crea were all improved significantly in the treatment group than control group.At last,two patients gave up their treatment,nine cases were cured,four cases died in treatment group.One patient gave up treatment,four cases were cured,ten cases died in control group.The total effective rate of the treatment group and control group was 60.0％,26.7％,the difference was significant(x2 =4.75,P＜0.05).Conclusion Xuebijing injection has very good clinical effect in multiple organ failure of paraquat poisoning,it is worthy of clinic application.

Objective To observe the effects of alendronate sodium and alfacalcidol on the parameters of atherosclerosis in old women with type 2 diabetes mellitus(T2DM ) complicating osteoporosis .Methods 28 old women with T2DM complicating osteopo-rosis were taken as the osteoporosis group and 25 old women with T2DM and without osteoporosis were taken as the control group .Alendronate sodium(70 mg/week) and alfacalcidol(0 .5 μg/d) were given to the osteoporosis group for 12 month .The pa-rameters of the bone mass density (BMD)of femoral neck and ,T value ,arkle brachial index (ABI) ,cardio-ankle vascular index (CAVI) and carotid artery intima-media thickness(IMT) ,blood fat and glycosylated hemoglobin were observed .Results Com-pared with the control group ,BMD ,T value and ABI in the osteoporosis group were significantly decreased (P<0 .01) ,while CAVI and IMT were obviously increased(P<0 .05) .Compared with before treatment ABI ,CAVI and IMT after 12 month treatment in the osteoporosis group had no significant changes ,but BMD and T value of femoral neck and ward triangle area were significantly increased(P<0 .05) ,the atherosclerosis degree after 12 months in the control group was significantly aggravated (P<0 .05) .BMD and T value had no obvious change .Conclusion Alendronate sodium combined with alfacalcidol for treating T 2DM complicating os-teoporosis can improve the atherosclerosis parameters and has certain role in preventing and treating cardiovascular and cerebrovas-cular diseases .

Objective To observe the effect of intellectual activity on the cognitive function of the elderly.Methods The longi-tudinal follow-up study and the cross-sectional study were undertaken among physical examination people.The intellectual activity group (92 cases,more than 60 years old)often to take part in any one or more of the following activities for 5 years and above:pla-ying chess,playing the game of go,playing mahjong,playing cards,playing checkers,calligraphy,painting,reading,etc.The frequen-cy of intellectual activity was more than once per week at least.According to gender,age and educational level,92 cases were screened out as the control group and the control group was not to take part in above-mentioned intellectual activities or occasionally attend.The respondents had no baseline cognitive impairment.MoCA,MMSE,ADL and self-made questionnaire were adopted to conduct the evaluation.One year later when health check-up,the above-mentioned scales were used to evaluate again.The score changes were compared between the control group and experimental group.Results When enrolling,there was no significant differences in the MoCA scores,MMSE scores and ADL scores between the two groups.One year later,the comparison of the MMSE scores and ADL scores between the control group and experimental group had no significant difference (P>0.05).The Mo-CA scores manifested 2 cases (2.2%)of below the critical value of cognitive impairment in the experimental group and 6 cases (6.5%)in the control group,reaching the mild cognitive impairment(MCI)standards,the difference between the two groups showed the statistical significance (P<0.05).Conclusion The intellectual activity may be beneficial for the prevention of cognitive decline of the elderly and the MoCA scale is more suitable for screening MCI.

AIM: To establish a method of determining the content of total saponin of Radix et Rhizoma Notoginseng in buccal tablets by HPLC. METHODS: Chromatographic conditions are as follows:Hypersil NH_2 column(4.6 mm?200 mm,5 ?m) as the stationary phase,acetonitrile-1% phosphate buffer solution(0-10 min,20:80;10-20 min,25:75;20-25 min,45:55;25-30 min,50:50) as the mobile phase,the velocity of flow is 1.0 mL/min,the temperature of coloum is 35(?C) and the detection wavelength is 203 nm. RESULTS: The HPLC method has good linearity in determining the total saponin of Radix et Rhizoma Notoginseng content,and we can detect four saponins at the same time,linear range,ginsenoside Rg1 0.093 75-6 ?g,ginsenoside Re 0.011 72-0.75 ?g,notoginsenoside R1 0.023 44-1.5 ?g and ginsenoside Rb1 0.140 6-9 ?g,the correlation coefficient r=0.999 9. CONCLUSION: The established method in this article is simple and convenient,with good reproductivity.We can detect four saponins in the total saponin of Radix et Rhizoma Notoginseng Buccal Tablets at the same time by this method.It can be used for quality control of the total saponin of Radix et Rhizoma Notoginseng Buccal Tablets.

Objective To investigate and analyze the depression situation and the influencing factors in elderly inpatients.Meth-ods The cross-sectional method was used to conduct the investigation on randomly selected 125 elderly inpatients by the geriatric depressive scale(GDS)and the general personal questionnaire.Results Among 125 elderly inpatients,41 cases were depressed eld-erly inpatients,accounting for 32.8%.Disease,marital status,length of hospital stay,self-care ability,medical expenses and hobbies were the influence factors of the elderly inpatients(P<0.05).Conclusion The prevalence of depression in the hospitalized elderly patients is relatively high,the geriatrics should pay attention to it.

In recent years,it is a tendency to substitute acute kidney injury (AKI) foracute renal failure (ARF)in the aspect of international nephrology and emergency medicine.Compared with the past,the causes for AKI in children at present has been transferred from primary kidney disease to multi-factors,moreover,the morbidity and mortality of AKI is staying at a high level.From the perspective of epidemiology this paper briefly analyses the common cause,mortality and morbidity of AKI in children.