Objective To observe changes in plasma level of brain natriuretic peptide ( BNP), left ventricular function in acute myocardial infarction (AMI) patients and discuss the effect of atorvastatin in these patients. Methods Eight patients with acute myocardial infarction hospitalized from October 2006 to December 2008 in our department were enrolled in the study. The AMI patients were further randomly assigned into the conventionsl treatment group ( n = 38 ) and atorvastatin treatment group ( n = 42 ). In the conventional group all patients took ACEI, beta-blocker, nitrate drugs, aspirin and low molecular beparin as their conventional therapy without medication taboo. In the atorvastatin group all patients took atorvastatin 20 mg per day for three months in addition to the conventional treatment. Twenty healthy individuals were testified by coronary arteriongraphy and ultrasonic cardiogram selected as controls ( n = 20). LVEDd, LVEF and left ventricular fractional shortening (LVFS) of 80 patients with AMI and 20 healthy controls were examined by ultrasonic cardiography and their plasma concentrations of BNP were measured. As to the conventional and atorvastatin group, the parameters were tested on the day of hospitalization (within 24 hours of AMI) ,also at the 3rd week and 3rd month after treatment. All data were analyzed statistically by SPSS12. 0. Results There were no significant changes in LVEDd, LVEF, LVFS after 3-week treatment compared to pre-treatment ( P ＞ 0.05 ), but significant changes after 3-month treatment ( P ＜0. 01 ) in 2 AMI groups. Moreover, LVEDd, LVEF, LVFS in the atorvastatin group were significantly greater than the conventional group (P ＜ 0.05). The plasma levels of BNP measured within 24 hours of AMI onset in two treatment groups were significantly higher than the control group. The plasma BNP level decreased significantly after 3 - week and 3-month treatment( all P value ＜0. 01 )in both treatment groups and atorvastatin group showed even lower BNP level with comparison to the conventional group( P ＜0.05 ). The plasma BNP in myocardial infarction patients after three months of treatment were negatively correlated with LVEF( r1 = -0.48 ,r2 = -0.58 ,P ＜ 0. 01 ) which implied a close relationship between BNP and heart function. Conclusions The changes of plasma BNP is earlier than that of heart structure. The long-treatment usage of atorvastatin may improve cardiac function, decrease the plasma BNP and convert ventricular remodeling,which benefit patients besides lipid managing. The plasma BNP could be used as a sensitive indicator of the treatment response and a useful index for evaluating the heart function and pronosis in AMI patients.

Objective To research on study attitudes of military medical university undergraduates. Methods The study attitudes of all the undergraduates were investigated and compared in different sorts by using homemade questionnaires. Results The general study attitudes of military medical university undergraduates were good. The undergraduates of different grades had not different study attitudes, neither did the undergraduates of different ages. The undergraduates of different sorts had different attitudes, so did the undergraduates of different genders and different specialties. Concretely, the undergraduates not recruited by Army, the men undergraduates, the undergraduates of long academic-year program and five-year military medico have better study attitudes. Conclusion The general study attitudes of military medical university undergraduates were good but there is still some space to boost. All sorts of steps should be taken to correct their study attitudes and advance their study effects.

Objective: To study the microwave assisted extraction process for saponins and isofraxidin in Radix Acanthopanacis Senticosi as a marker. Methods: Using the uniform design, the optimization process of microwave assisted extraction for saponins and isofraxidin in Radix Acanthopanacis Senticosi was gained by continuous microwave radiation. Results: Some parameters such as microwave power, radiation time, solvent concentraction, solvent consumption, pulverized degree and dipping time had interactions on the extraction of active constituents in Radix Acanthopanacis Senticosi. Conclusion: The optimization process was obtained which could be described as following: 510W as the microwave power, 30 min as the radiation time, 85% ethanol as the solvent, 1∶5 as the proportion of raw material to solvent, 40 mesh as the grinding degree and 0.5 hr as the dipping time. Meanwhile, the model developed were proved to be reasonable by the pilot experiment.

Object To study the microwave-assisted extraction process of Radix Polygoni Multiflori. Methods Using the uniform design, the optimization process of microwave-assisted extraction for total anthraquinones and 2, 3, 5, 4′- tetrahydroxystilbene-2-O-?-D-glucoside in Radix Polygoni Multiflori was gained by continuous microwave radiation. Results Some parameters, such as microwave power, radiation time, solvent concentration, solvent consumption and dipping time had interactions to the extraction of active ingredients in Radix Polygoni Multiflori. The best optimization process was obtained as following: 340 W as the microwave power, 10 min as the radiation time, 95% ethanol as the solvent, 1∶5 as the proportion of solid to liquid and 1 h as the dipping time. Conclusion The established models were proved to be reasonable by the verified experiment.

Object To study microcosmic mechanism about microwave-assisted extraction (MAE) to Radix Puerariae (RP) and Radix Caulis Acanthopanacis Senticosi (RCAS). Methods By transmission electron microscope, the structure changes about tonoplast in RP and RCAS were observed. Results The effect on tonoplast was differently made by MAE and conventional reflux extracting method. Conclusion The effective ingredients could be dissolved more easily with MAE than conventional reflux extracting method.

Antimalarials ; therapeutic use ; Artemisinins ; therapeutic use ; Child ; Drug Therapy, Combination ; Humans ; Malaria, Falciparum ; drug therapy

Adolescent ; Adult ; Case-Control Studies ; Female ; Humans ; Lymphoma, Non-Hodgkin ; blood ; diagnosis ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis ; Receptors, Urokinase Plasminogen Activator ; blood ; Young Adult

Objective To investigate the expression of human papilloma virus L1 (HPV L1)capsid protein in cervical lesions and different human papillomavirus ( HPV) subtypes, and to guide clinical triage management and best individual treatment.Methods Retrospective analysis of 2012 January to 2014 Janu-ary in Jiaxing Hospital of Traditional Chinese Medicine gynecology clinic for HPV L1 protein combined with HPV type, and liquid-based cytology test ( TCT) of 176 patients data.Results The positive expression rate of HPV L1 protein with TCT examination in the negative for intraepithelial lesion or malignancy ( NILM) , atypical squamous cells of undetermined significance( ASCUS) , low-grade squamous intraepithe lial lesion ( LSIL) , atypical squamous cells not except high lesion ( ASC-H) , high-grade squamous intraep-ithelial lesion ( HSIL) , and squamous-cell carcinoma ( SCC) was 28.99%, 44.19%, 64.44%, 22.22%, 12.50%, and 0, respectively.No significant differences were found between the NILM and ASCUS groups ( P >0.05) .The positive expression rate of HPV L1 protein in LSIL group was the highest, and it was sta-tistically significantly different from ASC-H and HSIL groups (χ2 =3.88,5.50, P <0.05) , and 0 expres-sion in SCC group.It suggests that the positive expression rate of HPV L1 protein decreased with the severi-ty of cervical cytology lesions.The top five diseases with positive expression of HPV L1 protein were inflam-mation (44.76%), cervical intraepithelial neoplasia (CIN)Ⅰ(52.94%), CIN Ⅱ(23.81%), CIN Ⅲ(8.33%), and squamous cell carcinoma (0); no significant difference was found between inflammation group and CIN Ⅰ group ( P >0.05 ); and statistically significant difference was found between CIN Ⅰgroup and CINⅡ, CINⅢgroup (χ2 =4.53,5.56, P <0.05), and 0 expression in SCC group.It sug-gests that the positive expression rate of HPV L1 protein increased with the increased level of cervical le-sions.The positive expression rate of HPV L1 protein in top five frequency of HPV infection was 60.78%in multiple infection, 17.65%in type 16, 50%in type 52, 66.67%in type 58, and 12.5%in type 18, re-spectively.The expression rate of HPV L1 protein of Type 16 infection was significantly lower than that of type 52, type 58 infection and multi infection(χ2 =4.15, P <0.05, χ2 =7.88,15.44, P <0.01).The positive expression rate of HPV L1 protein of type 18 infection was lower than the other three, but significant differences occurred only compared to multiple infection(χ2 =4.70, P <0.05).The rest had no significant difference( P >0.05) .Conclusions Detection of HPV L1 protein is of clinical value to evaluate the risk of cervical lesions.HPV L1 protein combined with HPV type and TCT detection is helpful for traffic man-agement and personalized treatment, and benefit patients with cervical lesions.

AIM: To study the microwave-assisted extraction process of forsythin from Forsythia Suspensa. METHODS: The optimization process for forsythin in Forythia Suspensa was gained by uniform design. RESULTS: The optimization process was obtained as following: 60% ethanol as solvent at the 680 W microwave power,40 min as the radiation time,5∶1(w/w) as the proportion of solvent to raw material and 30 min as the soaking time. CONCLUSION: In comparison with the traditional extraction,the forsythin content of target substance used the microwave-assisted extraction was higher than the former,and the extraction efficiency was also much better.

Objective To evaluate the effects of aqueous extract from Semen Lepidii seu Descurainiae(SLD) on myocardial hypertrophy induced by isoprenaline(ISO) and L-thyroxine(L-Thy) in experimental mice and rats.Methods The myocardial hypertrophy models of mice were prepared by daily sc ISO 2 mg/kg for 7 d and of rats by daily ip L-Thy 0.3 mg/kg for 10 d.The mice were given aqueous extract from SLD 6 and 12 g/kg or Metoprolol 0.06 g/kg by ig administration once a day for 7 d,then the changes of cardiac indexes,plasma concentration of cAMP,and angiontensin Ⅱ(AngⅡ) content in myocardium were measured.The rats were given the aqueous extract from SLD 4 and 8 g/kg or Captopril 0.02 g/kg once a day for 10 d,then the changes of cardiac indexes,plasma concentration of ALD,AngⅡ content in left ventricle,and hydroxproline(Hyp) level were measured.Results Compared with the normal mice,cardiac indexs,plasma concentration of cAMP,and Ang Ⅱ content were remarkably increased in the model group(P