Objective To investigate the relationship between the expression of endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) and the angiogenesis in human malignant melanoma (MM). Methods Tissue sections from 31 MM patients were examined. The protein expression of eNOS and VEGF was detected using immunohistochemistry technique and morphological quantitative analysis. Microvessel density (MVD) was also counted by immunostaining with anti-FⅧRAg antibody. Results (1)The positive staining of eNOS and VEGF was detected in 71.4% (24 cases) and 83.9% (26 cases) of MM respectively. As negative control there was no staining in the nevus pigmentosus tissue sections. (2)There was a positive correlation between the expression of eNOS and VEGF in MM. (3)The positive correlation was also found between the MVD and the expression of eNOS and VEGF in MM. The number of MVD was higher in MM than that in nevus pigmentosus tissue. (4)The lymphatic metastasis was not correlated with the expression of eNOS and VEGF. Conclusion The number of the MVD increases along with the high expression of eNOS and VEGF in MM.

OBJECTIVETo observe the therapeutic effect of Yangxue Tongluo Recipe (YTR) combined with immunosuppressive agents in the treatment of rheumatoid arthritis (RA).

METHODSTotally 88 RA patients were randomly assigned to the treatment group [47 cases, YTR combined Methotrexate (MTX) + Leflunomide (LEF) treatment] and the control group (41 cases, MTX + LEF therapy). All patients received 12-week treatment. Clinical symptoms and signs, laboratory tests [erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), and C reactive protein (CRP)], and adverse reactions were observed before and after treatment.

RESULTSThe total effective rate was 91.5% (43/47 cases) in the treatment group, and the total effective rate was 75.6% (31/41 cases) in the control group. There was statistical difference between the two groups (P < 0.05). The morning stiffness, the rest pain, the number of tender joints, the number of swollen joints, tender joint index, swollen joint index, ESR, RF, and CRP were significantly improved in the two groups after treatment (P < 0.01). Besides, clinical symptoms and signs, ESR, RF, and CRP were more improved in the treatment group after treatment, when compared with those in the control group (P < 0.05). Gastrointestinal discomfort was the main adverse reaction in the two groups, but the occurrence was lower in the treatment group than in the control group (P > 0.05).

CONCLUSIONSThe clinical efficacy of YTR combined MTX + LEF in the treatment of RA was better than using Western medicine alone. It was more safe with less adverse reactions.

Adult ; Antirheumatic Agents ; therapeutic use ; Arthritis, Rheumatoid ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Immunosuppressive Agents ; therapeutic use ; Male ; Methotrexate ; therapeutic use ; Middle Aged ; Phytotherapy ; Treatment Outcome

Antineoplastic Agents, Phytogenic ; therapeutic use ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Phytotherapy ; Plants, Medicinal ; chemistry

Abnormal eye blinking in children is a disease of frequent eye blinking in children, more than 15 times per minute, without facial spasms and neurological diseases, with relatively independent clinical symptoms. If combining with organic or neurological disease, we consider it as children eye blinking syndrome. It's a common and frequently encountered disease in pediatric ophthalmology. The etiologies include body and psychological disorders, such as refractive errors, ocular surface and ocular adnexal disease, bad habits, lead pollution, tic disorders and so on. We think that most of abnormal eye blinking in children firstly caused by oculopathy, neurological disorders and psychological illness are the important factors for increasing or making it repeatedly happen. Recognizing and identifying the etiologies of abnormal eye blinking in children, so as to take a targeted theray and avoid misdiagnosis and delayed treatment.

Humans ; Integrative Medicine ; Lupus Erythematosus, Systemic ; therapy ; Medicine, Chinese Traditional ; methods ; standards

Abscess ; pathology ; Adenoma ; metabolism ; pathology ; Adenoma, Sweat Gland ; metabolism ; pathology ; Biomarkers ; metabolism ; Breast Diseases ; pathology ; Breast Neoplasms ; metabolism ; pathology ; Female ; Fistula ; pathology ; Humans ; Keratin-7 ; metabolism ; Keratins ; metabolism ; Mucin-1 ; metabolism ; Nipples ; pathology ; Paget's Disease, Mammary ; metabolism ; pathology ; Receptor, ErbB-2 ; metabolism ; Sweat Gland Neoplasms ; metabolism ; pathology

Objective To assess the role of intervention of dexmedetomidine in lung tissue and plasma of septic rats induced by lipolysaccharide.Methods Forty SD rats weighing 250-300 g were divided into 4 groups:control group (n =10)with saline (1 ml·kg-1·h-1 )infused through the tail veins of rats for 6 h;dexmedetomidine (DEX)group (n =10)with dexmedetomidine,injected 6.5 μg ·kg-1·h-1 for 10 min followed by 5 μg·kg-1·h-1 ,continuously infused through tail veins for 6 h;li-polysaccharide (LPS)group (n = 10 )with lipolysaccharide (7.5 mg/kg)infused through the tail veins of rats;LPS+DEX group (n =10),after infusion of LPS,rats were treated with dexmedeto-midine (bolus 6.5 μg·kg-1·h-1 ,10 min;5 μg·kg-1·h-1 ,continuous infusion)through the tail veins for 6 h.ELISA and Western blot were performed to assess the protein expression of IL-1,IL-6, TNF-αand TLR4;the wet/dry ratio (W/D)of lung tissue in each group was measured and the injury scores were recorded through the Murakami method.Results There was no statistical significance between the control group and DEX group in plasma level of inflammatory factors,pulmonary TLR4 expression,wet/dry ratio and injury score of lung tissues,however,compared to the control group, there was a significant elevation in the above-mentioned biomarkers,W/D and injury score of lung tis-sue (P <0.01).Compared to the control group,there was no statistical significance in the LPS+DEX group.Compared to the LPS+DEX group,the biomarkers mentioned above,W/D and injury score of lung tissues were significantly elevated in the LPS group (P <0.01).Conclusion The irrita-tion of LPS can trigger the elevation of inflammatory factors in plasma,TLR4 expression in lung tis-sues in rats,while the intervention of dexmedetomidine can significantly alleviate this tendency. Dexmedetomidine can suppress the inflammatory reaction and pulmonary edema in septic rats.

Objective To measure the concentration of the amniotic fluid EPO(AFEPO) and investigate the relationship between AFEPO levels and fetal hypoxia. Methods Their AFEPO and the umbilical vein serum EPO (UVSEPO) were measured thirty pregnancy induced hypertension pregnancies (PIH group) , 30 gestational diabetic pregnancies (Diabetic group) and 25 normal pregnancies (control group) were terminated by cesarean section by using a chemiluminescent enzyme labeled immunometric assay (CELIMA). Meantime, umbilical artery pH (UapH), base excess(UaBE) and UapO2 and so on were also measured. Results The mean concentrations of AFEPO and UVSEPO in PIH (1.42?0.33 and 1.63?0.37) and Diabetic group (1.53?0.25 and 1.81? 0.34) were significantly higher than those in control group (0.91?0.11 and 1.13?0.14), ( P

AIM:To evaluate the regulatory effect of microRNA-132 ( miR - 132 ) in human umbilical vein endothelial cell ( HUVEC) .
●METHODS: ln vitro cultured human umbilical vein endothelia cells in hypoxic environment for 6h, then maintained under normal oxygen condition for 3h, 6h, 12h, 24h. miR-132 and peroxisome-proliferator-activated receptor-γ coactivator-1α ( PGC-1α) expression was detected by quantitative Real - time polymerase chain reaction and Western blot analysis. Human umbilical vein endothelial cells transfected miR-132 mimic and miR-132 inhibitor( anti-miR-132 ) were measured by quantitative Real-time polymerase chain reaction and Western blot.
●RESULTS: miR - 132 and PGC - 1α expression was significantly ( P < 0. 01 ) upregulated in the hypoxic environment of cells at 3h compared with the normal oxygen condition. After cells transfection, the hypoxic environment the miR-132 and PGC-1α expression were markedly increased compared with the normal oxygen condition. The cells transfected miR- 132- mimic, the expression of the miR-132 and PGC-1α were higher than that of transfected anti-miR-132 and contrast group ( P<0. 01).
●CONCLUSION:miR-132 level is highly expressed in the HUVEC under hypoxia and may be an effect of regulation for PGC-1α.

Objective To investigate the value of acoustic radiation force impulse (ARFI) on evaluating the renal tubular atrophy and interstitial fibrosis of IgA nephropathy(IgAN).Methods 117 patients diagnosed with IgAN,and 80 normal cases were examined with conventional ultrasonography and ARFI,recording the shear wave velocities(SWV),the hemodynamics and the renal biopsy results to quantify the elastic changes of renal cortex,with the aim of assessing AFRI's advantages on early diagnosing the impair of kidney.Results The SWV value of renal cortex,renal medulla of IgAN group were smaller than that of normal group (P ＜0.05),while the collective system SWV value had no difference(P ＞0.05).According to the ROC curve,the cut-off value of SWV in renal cortex was 2.7 cm/s.The accuracy of the diagnosis was 71 ％,which was better than RI (61.9％).The SWV value of the renal cortex decreased with the different stage of 1,2,3,4,but stage 5 was larger than stage 4.The degree of renal tubular atrophy and interstitial fibrosis(T) can be classified by the SWV of renal cortex,and the SWV of T2 was larger than T1 (P ＜0.05).Conclusions By quantifying the elastic change of kidney,ARFI technology is able to distinguish the degree of renal tubular atrophy and interstitial fibrosis,diagnose renal impairment early and judge the clinical stages.Furthermore the sensitivity,specificity,accuracy of it are superior to hemodynamic.