Objective To construct adenovirus vector harboring CTLA4Ig-IRES2-I?B? gene for the study of enhancement blockade of T cell costimulatory pathway. Methods The IRES2 fragment was cloned in to the shuttle plasmid pAdtrack-CMV-CTLA4Ig, and then the human I?B? fragment was constructed with the link of the IRES2 to the pAdtrack-CMV-CTLA4Ig. Subsequently, adenovirus vector harboring CTLA4Ig-IRES2-I?B? was constructed by homologous recombination in E. coli BJ5183, and recombinant vector was packaged and propagated in 293 cells. Results The recombinant CTLA4Ig-IRES2-I?B? adenovirus was constructed by homologous recombination and identified by PCR and restrictive enzyme digestion methods. Conclusion The recombinant CTLA4Ig-IRES2-I?B? adenovirus may be used as a novel immunosuppressive agent for gene therapy in organ transplantation.

Objective:To investigate depression of elderly patients in general hospital Method:315 patients aged 60 or above in Tongji hospital were screened with CES-D (Chinese version of Center of Epidemiological Survey-Depression) Results:70 of them (22%) definitely had depression, 53 were suspected of depression Female had higher scores in CES-D than male The less educated had higher score in CES-D Multiple regression showed poor education as a risk factor for depression in elderly patients Discussion: Routine screening for depression in elderly patients in general hospital is necessary

Objective: to compare clinical effect of cognitive-behavior therapy and pharmacological treatment on chronic insomnia Method:48 patients with chronic insomnia were divided into 4 groups, receiving treatments of cognitive-behavior therapy, medication, placebo,and cognitive-behavior plus medication separately All of the subjects were assessed with polysomnogram over night and sleep diary Result:the three active treatments were more effective than placebo at post-treatment assessment On the eighth day of treatments, medication group and combined group showed improvement, sleep latencies were 20 min and 27 min, sleep efficacy were 80% and 82%, total sleep time were 381 min and 356 min, which were better than the results before treatments At the end of 8 weeks, cognitive-behavior group showed improvement in the above three indexes At the end of 8 months' follow-up, cognitive-behavior group still showed better effect in sleep latency 26 min, sleep efficacy 80%, total sleep time 378 min than those of 8 weeks While the indexes of the medication group and combined group could not maintain the effect of treatment Conclusion:medication shows a short-term effect on chronic insomnia, cognitive-behavior therapy shows long-term effect

Objective To realize the inter-network connectivity problem through one information-point. Methods According to the characteristics of twisted -pair connections, following the work principles and standards of Ethernet, the transformation of twisted-pair was rebuilt by tri-pass connecting twin-network. Results Each pair of thread groups was taken full advantage by achieving twin-network. Conclusion By comparing the two types of transformation methods, a set of practical solution is explored of handy, mobility, non-destructive and reversible.

Objective To introduce the aim,design principles,function and characteristic of LIS. Methods Through functionalized design of module,this net management system is created by composed application,sampling,check,count,verify,auditing,issuance,quality control,query,consume control as an entirety. Results The accurate test data,the timeliness report and the query convenience were increased. Conclusion The LIS can provide exact and quick medical service for patients.

Earthquake may result in various urologic problems by direct injury of urologic organs or by spinal cord injury.This article introduces the main urologic problems after earthquake,classified into early and long-term.Such as urinary retention,oliguria or anuria,renal injury,bladder and urethra injury,neurogenic bladder caused by spinal cord injury,et al.We analyze the causes of the problems and discuss the correct management principle,all kinds of rehabilitation measures to reduce the complication and sequela,also the problems should be cautioned during the long term follow-up.

Objective To investigate the anticancer efficacy of dihydromyricetin (DMY)in gastric cancer cells SGC7901. Methods The DMY was puritied using RP-HPLC was almost 98%.The cell viability was assessed by CCK-8 Assay.The cell ap-optosis was assessed by flow cytometry.Using ultrasonic extraction with neutral water and reversed-phase high-performance liquid chromatography,DMY was purified from rattan tea.Results By using RP-HPLC,the purified DMY is almost 99%.DMY inhibi-ted the growth of tumor cells in the low concentration(≤ 1 mmol)with a dose-dependent manner(P <0.05 ).Further study on DMY showed that it induced apoptosis in SGC7901 cell line with a dose-dependent manner.Conclusion DMY is a potential thera-peutic agent for gastric cancer.

Objective : To investigate the incidence of new HIV infection and its influencing factors among men who have sex with men ( MSM ) in China, so as to provide the evidence for formulating the AIDS control strategy. Methods : Cohort studies pertaining to new HIV infections among Chinese MSM populations were retrieved in Chinese and English electronic databases, including CNKI, Wanfang Data, VIP, PubMed, Web of Science, Embase and Cochrane Library, and the retrieval time was set to build the database until May 2021. A meta-analysis was performed to investigate the incidence of new HIV infection and its influencing factors among Chinese MSM populations. The publication bias was assessed using funnel plot and Egger's test, and the stability of outcome parameters was evaluated using sensitivity analysis. Results : A total of 850 publications were retrieved, and 43 eligible literatures ( 44 studies ) were included in the final analysis. The pooled incidence of new HIV infection was 4.93 ( 95%CI: 4.15 to 5.72) per 100 person-years among Chinese MSM populations. Egger's test showed a publication bias ( t=2.411, P=0.021). Subgroup analysis revealed high incidence of new HIV infection in east ( 5.21 per 100 person-years ), north ( 5.30 per 100 person-years ) and southwest (4.84 per 100 person-years) China, and the lowest incidence in northwest China ( 2.87 per 100 person-years ), and showed higher incidence in first-tier cities ( 5.12 per 100 person-years ) than in nonfirst-tier cities ( 3.89 per 100 person-years ). Syphilis infection ( HR=3.41 ), unprotected anal sex ( HR=2.52 ), multiple sexual partners ( HR=3.51 ), seeking sex partners in bars, public baths and parks ( HR=3.44 ), age of > 25 years ( HR=0.44 ), Han Ethnicity ( HR=0.35 ), awareness of AIDS-related knowledge ( HR=0.20 ) and provision of HIV preventive services ( HR=0.18 ) were associated with new HIV infection in Chinese MSM populations. In addition, publication bias was found in studies reporting syphilis infection ( t=4.841, P<0.001 ), unprotected anal sex ( t=4.114, P=0.006 ) and ethnicity ( t=-5.018, P=0.038 ). Conclusions The incidence of new HIV infection is high among Chinese MSM populations. Interventions targeting the factors affecting new HIV infections are required to reduce in the incidence of new HIV infections among Chinese MSM populations.

BACKGROUND:The anatomical morphology of the distal end of humerus is an important factor for restricting the treatment of fracture at the site, also for designing and improving of internal fixator and prosthesis. It is very significant to determine the anatomical parameters of the distal end of humerus for correcting the deformation, fixation of internal fixator and replacement of prosthesis.OBJECTIVE: To measure 17 anatomical parameters of the angles and structural width or depth related to the distal end of the humerus, verify their fitness with bone after implantations of internal fixator and prosthesis, and identify the association between the characteristics and the mechanical properties of the internal fixator and prosthesis.DESIGN: A repetitive measurement and comparative observation.SETTING: Affiliated Hospital of Nantong University.MATERIALS: The experiments were carried out in the Trauma Research Room, Affiliated Hospital of Nantong University from October 2005 to February 2006. Fifty-four pairs of humerus samples from adult antisepsis corpses were randomly selected (provided by the Department of Human Anatomy, Medical College of Nantong University), including 30 pairs from males and 24 pairs from females, while those with deformation and degeneration of elbow joint were excluded. METHODS: The soft tissues adhered to the humerus samples were all rejected, and the anatomical parameters of the distal end of humerus were measured with slide gaud (0.01 mm in precision, produced by Shanghai Measuring and Cutting Tool Works), calipers and goniometer. ① Measurement of angles related to the distal end of humerus: The measured parameters included the anteverted angle of the distal humerus, the lateral rotation angle of humerus trochlea,the anteverted angulation of humerus capitulum, the anteverted angulation of humerus trochlea, the condyle-shaft angle of humerus, and the internal rotation angle of the line of lateral-medial humerus epicondyle. ② Measurement of structural width and depth related to the distal end of humerus: The measured parameters included the horizontal diameter of the anterior end of humerus trochlea, the horizontal diameter of the inferior end of humerus trochlea, the sagittal diameter of humerus trochlea, the width between medial epicondyle and external epicondyle, the width between medial trochlea and lateral capitulum, the width of olecranon fossa, the depth of olecranon fossa, the width of medial epicondyle, the depth of sulcus for ulnar nerve, the width of humerus capitulum, and the sagittal diameter of humerus capitulum..MAIN OUTCOME MEASURES: Results of the angles and structural width or depth related to the distal end of the humerus.males and females in the anteverted angle of the distal humerus [(35.62±5.21)°, (96.22±5.29)°], the lateral rotation angle of humerus trochlea [(5.22±1.15)°, (5.46±1.33)°], the anteverted angulation of humerus capitulum [(48.35±8.78)°,(49.65±9.12)°], the anteverted angulation of humerus trochlea [(33.08±7.15)°, (34.45±7.52)°], the condyle-shaft angle of humerus [(80.12±5.22)°, (80.17±5.45)°], and the internal rotation angle of the line of lateral-medial humerus humerus: There were no significant differences between males and females in the horizontal diameter of the anterior end of humerus trochlea [(21.40±4.21), (21.12±4.34) mm], the horizontal diameter of the inferior end of humerus trochlea [(23.54±4.52), (23.31±4.43) mm], the sagittal diameter of humerus trochlea [(23.91 ±4.85), (23.55±4.63) mm], the width between medial epicondyle and external epicondyle [(58.23±6.44), (55.32±6.55) mm], the width between medial trochlea and lateral capitulum [(45.36±5.21), (42.15±5.03) mm], the width of olecranon fossa [(24.56±4.25), (25.34±4.42) mm],the depth of olecranon fossa [(13.43±2.52), (12.95±2.13) mm], the width of medial epicondyle [(14.35±3.32), (13.02±2.96) mm], the depth of sulcus for ulnar nerve [(8.53±2.96), (7.90±2.54) mm], the width of humerus capitulum [(18.05±2.87), (16.51±2.75) mm], and the sagittal diameter of humerus capitulum [(19.59±3.84), (19.12±3.55) mm] (P＞0.05).CONCLUSION: The anatomical parameters of the distal end of humerus should be considered in performing correction of elbow joint, internal fixation and prosthesis replacement in order to get better recovery of elbow joint function.