Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Animals ; Child ; Female ; Furunculosis ; therapy ; Humans ; Male ; Middle Aged ; Young Adult

Acupuncture Points ; Acupuncture, Ear ; Adult ; Aged ; Combined Modality Therapy ; Female ; Humans ; Laser Therapy ; Male ; Middle Aged ; Urination Disorders ; therapy

Concurrent chemoradiation has been the main treatment of locally advanced non-small cell lung cancer (LA-NSCLC).However, there is a controversy whether giving the induction chemotherapy before treatment.The survival advantage of consolidation chemotherapy doesn't reach a consensus.When the dose of initial chemotherapy is insufficient, consolidation chemotherapy is necessary.With the development of radiotherapy and the renewal of the chemotherapy drugs, concurrent chemoradiation is expected to bring better survival benefit for the patients with LA-NSCLC.

Objective The metabolomics methods were applied to discover the serum differences metabolites in patients with lupus nephritis (LN),and to establish the diagnostic model and study the metabolic pathways change due to the interference of LN and explore the pathogenesis of LN.Methods Positive and negative ions mode of ultra high performance liquid chromatography-high resolution mass spectrometry (HPLS-HRMS) were used to analyze the serum samples of 38 LN patients and 35 healthy controls,respectively.Both univariate and multivariate data analysis were performed with SPSS software (V17.0,Chicago,USA) and SIMCA-P+ package (V12.0,Umetrics,Sweden),respectively.A supervised pattern recognition method,orthogonal partial least squares-discriminant analysis (OPLS-DA),was employed to study the metabolomics differences between control group and LN patients group.The metabolic pathways were analyzed by Metabo Analyst 3.0.Results LN patients and healthy controls could be well separated by OPLS-DA model Q2 of 0.583.Eighteen compounds (theophylline,oxidized glutathione,capric acid,norvaline,hippuric acid,sphingosine,sorbitol,cortisol,pyrrole-2-carboxylic acid,deoxycholic acid,tryptamine,5-aminolevulinic acid,glucose 6-phosphate,riboflavin,cortisone,taurine,creatinine,glycerophosphocholine) were initially identified as potential serum metabolic markers to distinguish LN patients from healthy people.Alternated metabolism pathways included citrate metabolism,pyrimidine metabolism and amino acid metabolism.Conclusion Through analysis of the metabolic pathways of different metabolites,the significant impact of LN on citrate metabolism,pyrimidine metabolism and amino acid metabolism pathways are identified.Some of the different metabolites are involved in the process of immunization.These results will benefit the early intervention and treatment of LN and the research of the mechanism of pathogenesis.

Objective　To observe the change of lipidemia and lipoprotein in wistar rats with fluorosis.Methods　14 wistar rats were fed normal food with hyper concentration of NaF water (100 mg/L) for six months,the TC,TG,HDL-c,LDL-c,APO AI APO B and AI,R-CHE were measured in serum.Results　The result show that there are an increace of T C,TG,HDL-c,LDL-c,APO AI，APO B and AI,R-CHD compared with normal rats (P <0.05).Conclusions　The result indicate that the fluorosis can increase the lipidemia and lipoprotein in rats with fluorosis and lead to athero selerosis.

Acupuncture Points ; Acupuncture, Ear ; methods ; Adolescent ; Adult ; Child ; Enuresis ; therapy ; Female ; Ginger ; Humans ; Male ; Medicine, Chinese Traditional ; Moxibustion ; methods

AIM:To evaluate the therapeutic effect of limaixian on genitourinary infections.METHODS:98 cases of genitourinary infections were randomly divided into 3 groups:treatment group,limaixian 250mg bid;control group A,roxithromycin 150 mg bid;control group B,amoxicillin 500mg tid.The therapeutic courses of 3 groups were 7 days.RESULTS:The total effective rate,pathogenic microbe clearance and adverse drug reactions(ADRs) in treatment group were 82.86％ ,81.82％ and 2.86％ respectively.The results were better than those in control group A and B.CONCLUSION:Limaixian is satisfactory in effect,mild in ADRs and broad in antimicrobial spectrum for treatment of genitourinary infections.It is worth extending.

The biological characteristic of carcinomas is determined both by the stromal microenvironment and the oncogene or anti-oncogene.The tumor stroma is also known as the reactive stroma which is composed of base member,immunocell,capillary,fibroblasts and ECM.Fibroblasts are the majority of tumor stromal cells.The relationship between fibroblasts and the initiation,progression of carcinoma has being in the spot light.In this review,we summarized the advance in the study of carcinoma-associated fibroblasts.

Objective To investigate the stabilization of betaine on the enzyme activity of ALT in human serum at different temperatures, and to evaluate the matrix effects of human serum with betaine among different analytical systems. Methods The enzyme activity of ALT in human serums with l mol/L betaine stored at 37 ℃, 25 ℃, 4 ℃ and - 20 ℃ respectively were measured. The matrix effect experiment was carried out according to the procedures specified in Clinical and Laboratory Standards Institute (CLSI)document EP14-A. Four analytical systems were the candidates for evaluation, and Roche Diagnostics (Roche)-Hitachi analytical system served as the reference system. The enzyme activity of ALT of 40 patient specimens and 3-level specimens ( high, medium and low) with 1 mol/L betaine were measured using the evaluated methods and the reference method respectively. The linear regression analysis was performed to compare the mean y value of 3-level specimens with the statistically defined limits ( the two-tailed 95% prediction interval) derived from 40 patient specimen data. Results The enzyme activity of ALT could be stabilized with betaine in serum, which remained to be 99% for 48 h at 37 ℃, 98% for4 d at 25 ℃, 97%for 4 weeks at 4 ℃, and 99% for 4 months at - 20 ℃. The enzyme activity of control serum decreased to 42%, 86%, 71%, 79% respectively. The mean y values of three specimens with the betaine were within the 95% prediction interval of the estimated mean y values, which suggested no obvious matrix effects in serum. Conclusion Betaine can be used in the development of enzyme ALT calibrator as its stabilizer.