OBJECTIVE: To determine imperatorin as the active ingredient of Angelica dahuricae in Jiedu tongqiao pills by HPLC. METHODS: The separation was performed on Waters Symmetryshielb33 C18(250 mm?4.6 mm,5 ?m) column with detection wavelength of 248 nm. The mobile phase consisted of methanol-water (65 ∶ 35) with flow rate of 1.0 mL?min-1.RESULTS:The linear range of imperatirin were 0.15～1.35 ?g. The average recovery was 100.4%(RSD=1.89%,n=9).CONCLUSION: The method is simple, accurate and reproducible for quality control of Jiedu tongqiao pills.

【Objective】 To observe the effect of acupuncture on immune organs(thymus and spleen) and T lymphocyte subtypes of CD3,CD4 and CD8 in rats with dysmenorrhea.【Methods】Thirty SD rats were randomized into normal control group,model group and electroacupunture(EA) group.Except the normal control group,rats in other groups received intraperitoneal injection of diethylstilbestrol and oxytocin to induce dysmenorrhea.EA group received EA on bilateral acupoints of Sanyinjiao and Guanyuan additionally.After 10-day treatment,body-stretching rate,the pathological changes of thymus and spleen,and blood CD3,CD4 and CD8 levels in the three groups were observed.【Results】In the model group,body-stretching rate was 100%,the pathological changes of thymus and spleen were obvious,and blood CD3 and CD4 levels were decreased as compared with the normal control group(P

Objective To develop a clinical intelligent management system of dressing consumables to realize precision consumables management without increased manpower consumption.Methods The system was composed of four parts of storage box,display screen,detection unit and control processor.The storage box consisted of storage units,identification unit and an input panel.The storage unit included a box body,a lid and an electronic lock for locking the body and lid,and the lock was connected with the control processor.Results The system recognized the medical prescription automatically,and then corresponding dressing consumables were packed and ejected accordingly.Conclusion The system decreases the costs for time,manpower and medical service,and thus is worthy promoting practically for precision hospital consumables management.

Pulmonary infections are the most common complications in immunocompromised host (ICH).The immune system of ICH was in inhibitory condition because of long-term use of immunosuppressive agents,such as glucocorticoids,antimetabolite medicine and alkylating agents.Therefore,compared with the immunocompetent host,the condition is more critically serious in ICH with more difficult diagnosis and treatment,and higher motality after pulmonary infections.Early identification of pathogens and early treatment are helpful for improving the prognosis and decreasing mortality.

Objective It is clinically insufficient to assess the severity and prognosis of neonatal asphyxia only according to Apgar score and blood gas measurement. In order to find more sensitive and specific indexes, the clinical significance of cord blood lactate in neonates with asphyxia was studied. Methods The lactate level and the pH value of cord arterial bloot were determined in 31 term infants with perinatal asphyxia (further divided into the mild and the severe asphyxia groups) and 30 normal neonates (the control group). At the 14th day after birth, 20-item neonatal behavioral neurological assessment (NBNA) was taken for each subject. Results The levels of cord blood lactate in the mild and severe asphyxia groups [ (6.42 + 0.14) and (10.77 + 0.12) mmol/L] were significantly higher than that in the control group [(4.20 +0.15) rmmol/L] ( P ＜ 0.01). The pH values in the mild and severe asphyxsia groups weremarkedly lower than that of the control group [(7.16+0.07) vs (7.18+0.11); (7.04+0.09) vs (7.18+0.11)]( P＜ 0.01 ). There were significant differences in the lactate level and pH value between the mild and severe asphyxia groups( P ＜0.01). The lactate level was negatively correlated with the pH value and the score of NBNA ( r = -0.76 and-0.85, respectively, both P ＜ 0.01). Conclusions Cord blood lactate may be a useful index in evaluating the severity and short-term prognosis in neonates with perinatal asphyxia.

OBJECTIVEBy inhibiting AML1 -ETO fusion gene expression in Kasumi-1 cells with RNAi, to investigate the changes in cell proliferation and cell cycle.

METHODSThe small interference RNAs (siRNAs) specifically targeting the AML1 -ETO fusion gene were synthesized in vitro and transfected into Kasumi-1 cells by electroporation, the non-specific siRNAs transfected cells were taken as control. EGFP plasmid was transfected into Kasumi-1 cell and the transfection efficiency was detected by FCM. Inhibitory effect of siRNAs were detected by real-time RT-PCR and Western blots. Cell proliferation was measured by CCK-8 assay. DNA content was detected by PI assay.

RESULTSThe transfection efficiency was 44.5%. The AML1 -ETO specific siRNAs inhibited AML1 -ETO expression at both mRNA and protein levels. The cell proliferation rate in siRNAs treated group was lower than that in control group 72 h after transfection [(47.90 +/- 0.02)% vs (66.90 +/- 0.08)% , P < 0.05]. The cell cycle was blocked at G1 phase 72 h after siRNAs treatment, the cell proportion in G1 phase being 38.3% and 31.6% in control group, while in G2/M phase being 1.8% and 2.4% respectively.

CONCLUSIONSThe synthesized siRNAs can inhibit AML1 -ETO fusion gene expression. AML1 -ETO specific siRNA induced the decline of AML1 -ETO fusion protein in Kasumi-1 cell, and then caused the cell cycle blocked in G1 stage and eventually inhibited the cell proliferation.

Cell Cycle ; genetics ; Cell Line, Tumor ; Cell Proliferation ; Core Binding Factor Alpha 2 Subunit ; genetics ; metabolism ; Humans ; Leukemia ; genetics ; metabolism ; pathology ; Oncogene Proteins, Fusion ; genetics ; metabolism ; RNA Interference ; RUNX1 Translocation Partner 1 Protein ; Transfection

OBJECTIVEIn order to characterize the feature of stress response induced by stressor with both physical and psychological natures, the effects of water restriction performed in different experimental modes on the physiological parameters, psychological behavioral manifestations and brain c-Fos expressions were observed and compared.

METHODSFifty-eight male Wistar rats were used and randomly divided into three experimental groups (n = 18 for each) and a control group (n = 4). In control group, the rats were allowed to access drinking water freely at all experimental period. In the experimental groups the water supply to the rats was restricted. In timed water supply (TW) group, the water was supplied twice a day, 10 min for each in fixed hours every day. In empty bottle-served (EB) and water-restricted (WR) groups, the water was served only once a day for 10 min, either in the early morning or evening, and in the other time point scheduled for water supply only an empty bottle without water was provided in the EB group and nothing was given in the WR group. The quantities of drank water and eaten food, weight-gaining, and behavior score were observed every day. The serum level of corticosterone was assayed and the rats were sacrificed with fixative perfusion of 3 d, 7 d or 14 d, respectively, following water restriction (n = 6 for each time point in each group). The brain c-Fos expressions were examined with immunohistochemical method.

RESULTSThe slow down of weight-gaining, rise of serum corticosterone level, occurrence of psychological behavioral manifestations of unpeaceful restlessness such as exploring and attacking, enhance of c-Fos expression in the subfornical organ (SFO), median preoptic nucleus (MnPO), area postrema (AP), hypothalamic paraventricular nucleus (PVN), supraoptic nucleus (SON), medial (MeA) and central (CeA) amygdaloid nucleus and ventrolateral septum (LSV) were noticed in both EB and WR groups, except the nucleus of solitary tract (NTS) in which the Fos expression was decreased. The changes of Fos expression in most of nuclei in EB group began at day 3, at least persisted till day 7, and backed down at day 14, while in WR group, similar changes started at day 7 and reached its peak at day 14. In TW group, only the concentration of corticosterone at day 7 was slightly increased and the rest indexes observed were unchanged.

CONCLUSIONThe results indicate that water restriction induces physical as well as psychological stress responses. And the water restrictions experimentally executed in different modes result in different manifestations of behavioral response and brain immediately early gene expression in discrete brain nuclei/regions.

Adult ; Female ; Humans ; Hypertension, Portal ; diagnosis ; etiology ; genetics ; Liver Cirrhosis ; complications ; diagnosis ; genetics ; Male ; Middle Aged ; Models, Statistical ; Nitric Oxide Synthase ; genetics ; Polymorphism, Genetic ; Prognosis ; Prospective Studies

Acute cellular rejection (ACR) is a common complication after lung transplantation, which is mainly caused by the immune response of T lymphocytes recognizing the major histocompatibility complex on the cellular surface of grafts. It is currently considered as the main pattern of acute rejection. ACR is not only a direct cause of death of recipients, but also a high-risk factor for chronic rejection after lung transplantation. Nevertheless, it is a challenging task to deliver the diagnosis and treatment of ACR following lung transplantation. In this article, new progresses on the risk factors, pathogenesis, diagnosis and treatment of ACR in lung transplant recipients were summarized, aiming to improve the diagnostic and treatment efficiency of ACR and prolong the survival of recipients.

Objective:To investigate the morphological feature and clinical significance of MRI around tumor after drug-eluting bead transcatheter arterial chemoembolization (DEB-TACE) of primary hepatocellular carcinoma.Methods:We reviewed and analyzed the data of hepatocellular carcinoma patients admitted from January 2017 to December 2018 in the Department of Radiological Intervention of the First Affiliated Hospital of Zhengzhou University. A total of 42 patients were enrolled, including 35 males and 7 females, aged (57.0±11.9) years. For the first time after operation, MRI enhancement showed peri-tumor margin enhancement as the starting point of follow-up. Follow-up and measure enhanced edge thickness, delayed enhancement, progression or remission data.Results:A total of 49 tumors and 84 peritumoral enhancement margins were included in 42 patients, with 30 sharp type , 40 rough type and 14 nodular type. The thickness of sharp type is less than that of rough type and nsodular type, and the differences were statistically significant (all P<0.05). The sharp type is the majority of the tumors with maximum diameter <5 cm, rough type and nodule type are the majority of tumors with maximum diameter ≥5 cm. Most of the sharp type are continuously enhanced, while the rough type and nodular type are not. Most sharp type relief (93.3%, 28/30), while rough type (80.0%, 32/40) and nodular type ( n=12) are mostly of deterioration, the differences are statistically significant (all P<0.05). Conclusion:Compared with the rough type and nodular type, the sharp type usually occurs in smaller tumors and more prone to local mitigation in the enhanced morphology around MRI after DEB-TACE in primary hepatocellular carcinoma.