Congresses as Topic ; Head and Neck Neoplasms

Objective To establish a method for the determination of arsenic in urine by microwave digestion-oscillopolarographic method. Methods The urine samples were treated by microwave digestion. After being reduced to As3+,total arsenic in the samples was determined by oscillopolarographic method in the system of H2SO4-KBr-Se4+. The experiment conditions were optimized. Results The linear range of the method was 0-50 ?g/L(r=0.999 8),the limit of detection was 0.67 ?g/L,the relative standard deviation was 1.44%,and the rates of recovery were 95.0% -103.0%. Conclusion The method is simple,rapid,accurate and applicable to the determination of arsenic in human urine,which presents an advantage of low losing of arsenic in the treatment and determination.

Objective To evaluate the effects of mechanical stretch preconditioning on pathological stretch-induced damage to type Ⅱ alveolar epithelial cells.Methods A549 cells cultured in vitro were randomly divided into 6 groups (n =5 each)using a random number table:control group (Ⅰ group),pathological stretch group (Ⅱ group) and different time mechanical stretch preconditioning + pathological stretch group (group Ⅲ-Ⅵ).A549 cells were exposed to 20％ cyclic stretch at 0.3 Hz for 6 h in Ⅱ group.In Ⅲ-Ⅵ groups,A549 cells were exposed to 5％ cyclic stretch at 0.3 Hz for 15,30,60 and 120 min,respectively,and then exposed to 20％ cyclic stretch at 0.3 Hz for 6 h.After the end of pathological stretch,the apoptosis in cells was detected using flow cytometry and was calculated.F-actin expression was determined using flow cytometry.Results Compared with group Ⅰ,the apoptosis rate was significantly increased,and the expression of F-actin was up-regulated in Ⅱ group.Compared with group Ⅱ,the apoptosis rate was significantly increased in groups Ⅳ-Ⅵ,and the expression of F-actin was up-regulated in groups Ⅴ and Ⅵ.Conclusion Preconditioning with mechanical stretch (30-120 min) can mitigate pathological stretch-induced damage to type Ⅱ alveolar epithelial cells.

Objective To investigate the effects of propofol administered before, with or after lipopolysaccharide (LPS) on the acute lung injury (ALI) induced by IPS in rats. Methods Seventy-six male Wistar rats weighing 250-290 g were randomly divided into 5 groups : (A) control group received only normal saline (n = 8); (B) LPS group received LPS 8 mg?kg-1 iv (n = 17); (C, D, E) propofol group-Ⅰ,Ⅱ, Ⅲreceived propofol (a bolus of 5 mg?kg-1 followed by infusion at 10 mg?kg-1?h-1) 1 h before (group C, propofol - Ⅰ , n = 17) , simultaneously with (group D, propofol-Ⅱ, n=17) or 1h after LPS administration (group E, propofol-Ⅲ , n = 17) . The animals were observed for 5h after LPS administration for MAP monitoring and mortality and then killed. The lungs were immediately removed for determination of expressions of nitrotyrosine protein and iNOS mRNA, wet / dry lung weight ratio and pulmonary permeability index (PPI). The lungs were also lavaged. The bronchoalveolar lavage fluid (BALE) was collected for measurement of TNF-?, NO and protein contents. Results In group C and D propofol given before and simultaneously with LPS significantly inhibited the increase in nitrotyrosine protein and iNOS expression induced by LPS, improved MAP, reduced 5h mortality rate, decreased PPI and protein, NO and TNF-?contents in BALF compared with group B (P

Hepatoblastoma (HB) is the most common malignant tumor of the liver in children,and it is most prevalent in children under 5 years old.The overall prognosis of childhood HB has been improved significantly due to multidisciplinary team approach consisting with surgical resection and combination chemotherapy.Nevertheless,the clinical outcomes of metastatic and relapsed HB remain to be poor.This paper makes a brief review on the pathogenesis,diagnosis and treatment of HB.

Child ; Child, Preschool ; Humans ; Leukemia ; immunology ; prevention & control ; Vaccination

Objective The aims were to investigate the value of 18F-fluorodeoxyglucose (FDG) PET/CT in the diagnosis of bronehioloalveolar carcinoma (BAC) and its metabolic and anatomic features in differentiating from non-BAC adenocareinoma (non-BAC AC ). Methods This was a retrospective 18F-FDG PET/CT study on a consecutive series of 87 patients (32 BAC, 55 non-BAC AC) with 110 pathology-proven lesions. The maximum standardized uptake value ( SUVmax) was calculated for all lesions. Tumor's location, morphology and margins, internal structures were analyzed on CT. Statistical analysis compared the mean SUVmax between the two groups, analysed the relationship between tumor subtype and features on CT and compared the diagnostie aeeuraeies with PET alone, CT alone and PET/CT. The t-test, McNemar test, Fisher exact test were used to analyze the data using SPSS 12.0. Results Significant differences were found between mean SUVmax in a total of 47 lesions with BAC and 63 lesions with non-BAC AC (1.51±0.17 vs 6.28± 3.04, t=-10.374, P <0.0001 ). Pure ground glass density, which was foued in BAC, was the most significant CT feature in distinguishing tumor types ( Fisher exact test, P<0.0001 ). Diagnos-tic accuracies were 88% (28/32) with PET/CT, 47% (15/32) with PET and 66% (21/32) with CT. Differences in aeeuraeies between PET and PET/CT and between CT and PET/CT were statistically signifi-cant (P= 0.001,0.039 ). Conclusions Diagnostie accuracy can be higher by understanding the function-al eharaeteristies on PET and anatomical features on CT. The presence of persistent ground glass in a lesion on CT is a significant feature for BAC and should raise the suspicion of this tumor type even in cases of low 18F-FDG activity.

Helicobacter Infections ; complications ; Helicobacter pylori ; Hematologic Diseases ; complications ; Humans

Objective To evaluate the protective effects of lung protective ventilation on the lungs in patients undergoing radical resection for esophageal cancer.Methods Sixty-eight patients of both sexes,aged 40-64 yr,of ASA physical status Ⅰ or Ⅱ,with body mass index 17-40 kg/m2,scheduled for elective radical resection for esophageal cancer,were randomly divided into conventional ventilation group (CV group,n =34) and protective ventilation group (PV group,n =34) using a random number table.Double lumen tube was inserted after induction of anesthesia,an anesthesia machine was connected,and the patients were mechanically ventilated.In group CV,VT was set at 10 ml/kg during two-lung ventilation,and VT was set at 7 ml/kg,and I ∶ E was set at 1 ∶ 2 during one-lung ventilation.In group PV,VT was set at 7 ml/kg during two-lung ventilation,VT was set at 5 ml/kg,I ∶ E was set at 1 ∶ 2,and PEEP was set at 10 cmH2O during one-lung ventilation,and bilateral lung recruitment maneuver was performed every 45 min.Before induction of anesthesia,and at days 1,3,and 5 after surgery,blood gas analysis was performed,and Clinical Pulmonary Infection Score was recorded.Before induction of anesthesia,and at 5 days after surgery,point-of-care testing for pulmonary function was performed,and percentages of maximum ventilatory volume (MVV％),forced vital capacity (FVC％),and forced expiratory volume in 1 second (FEV1％) were recorded,and FEV1/FVC was calculated.The development of respiratory failure,pulmonary atelectasis and incision infection was recorded within 5 min after surgery.Results Compared with group CV,MVV％,FVC％,FEV1％ and FEV1/FVC were significantly increased,Clinical Pulmonary Infection Score was decreased at each time point after surgery,SaO2 and PaO2 were increased,and no significant changes were found in pulmonary atelectasis and incision infection in group PV.Conclusion Lung protective ventilation can effectively protect the lungs in the patients undergoing radical resection for esophageal cancer.