1.Expression of Senescence Markers Increased in the Frontal Lobes of Obese Rats
Dwi Cahyani Ratna Sari ; Mawaddah Ar Rochmah ; Wiwit Ananda Wahyu Setyaningsih ; Josephine Debora ; Dhite Bayu Nugroho ; Nur Arfian
Malaysian Journal of Medicine and Health Sciences 2020;16(Supp 3,June):62-66
Introduction: Obesity has been demonstrated to induce oxidative stress and inflammation processes that lead to
senescence. Obesity-induced cellular senescence in the brain is still widely investigated. This study aimed to investigate the expression of senescence markers in the frontal lobes of obese rats. Methods: Three groups of rats: control,
Obese-2 (Ob-2), and Obese-4 (Ob-4) were observed. Control rats were fed with a standard diet for one month. In
contrast, Ob-2 and Ob-4 rats were fed with a high-fat diet daily for two and four months, respectively. After being
sacrificed, the rats’ brains were dissected out then the frontal lobes were used for RNA extraction. Reverse transcriptase PCR of p-16, p-21, and beta-actin was performed to investigate the relative expression of the senescence
markers. Results: Ob-2 and Ob-4 groups had significantly increased body weight after being fed with a high-fat diet
for two and four months, respectively. The mRNA expressions of p-16 and p-21 in the frontal lobes of three groups
showed similar patterns. The ob-4 group had the highest mRNA expressions of both p-16 and p-21. In comparison
to control and Ob-2 groups, the mRNA expressions of p-16 and p-21 were markedly increased. Furthermore, the
mRNA expressions of p-16 and p-21 between control and Ob-2 groups were comparable. Conclusion: Increased
senescence markers in the rats’ frontal lobes were observed as the chronic effect of obesity.