1.Inhibition of Sanggenon G Isolated from Morus alba on the Metastasis of Cancer Cell
Long CUI ; Hyunsun LEE ; Wonkeun OH ; Jongseog AHN
Chinese Herbal Medicines 2011;03(1):23-26
Objective An organic layer prepared from the cortex of Morus alba (Moraceae) was studied in order to identify the active compounds for heparinase. Methods Bioassay-guided fractionation resulted in the isolation of sanggenon G Results The compound showed inhibitory activity with IC50 of 3.7 μmol/L on heparinase in vitro as well as 24 μmol/L in invasion assay using MDA-MB231 cells. Sanggenon G also had the moderate cytotoxicity at SW 620 (colon) and ACHN (kidney) cancer cell lines with IC50 of 10.96 and 13.44 μmol/L, respectively. Conclusion This is the first time that prenylated flavonoid sanggenon G is described as heparinase inhibitor. Besides, this flavonoid would be expected to be a metastasis inhibitor of cancer cells and also a valuable reagent to explore the mechanism of heparinase/heparanase-mediated metastasis.
2.Protein Tyrosine Phosphatase 1B Inhibitors from Plantago asiatica
Long CUI ; Hyunsun LEE ; Jongseog AHN ; Guangxin YUAN ; Yanan SUN
Chinese Herbal Medicines 2011;03(2):136-139
Objective To identify the active compounds for protein tyrosine phosphatase 1B (PTP1B) from the seeds of Plantago asiatica. Methods Bioassay-guided fractionation resulted in the isolation of iridoid glucosides (1-5) with PTP1B inhibitory activity. Results Five compounds were identified as desacetylhookerioside (1), melittoside (2), geniposidic acid (3), 10-O-acetyl-geniposidic acid (4), and alpinoside (5). Conclusion Isolated compounds 3-5 inhibit PTP1B with IC50 values ranged from (16.3 ± 1.1) to (19.8 ± 1.2) μmol/L.