Objective The purpose of this paper is to study the relationship between blood concentration and brain tissue drug concentration by different dose of TMX chemotherapy acute lymphoblastic leukemia in mice. Methods 4 weeks, health Kun Ming mice 80: establishment acute lymphoblastic leukemia mice model,20 mice were randomly selected to take the femur bone marrow biopsy bone marrow OK for model verification; the remaining 60 acute lymphoblastic leukemia mice were allocated randomly 6 groups of 10 mice in each group, respectively A, B, C, D, E, F groups. And collected blood 0.5 ml and brain tissue 0.4 g individually at 0.5 hour in every group. We used supernatant of centrifugation blood and brain homogenate to detected drug concentration by fluorescence polarization immunoassay. Results The mean blood concentration of MTX of six groups A, B, C, D, E, F are (39.08±5.18) μmol/L, (15.86±1.02)μmol/L, (8.67± 5.43)μmol/L, (68.29±5.19)μmol/L, (29.55±6.22)μmol/L, (13.98±1.12)μmol/L, respectively. Compared the mean blood concentration of MTX of each group there are statistical significance (P<0.05). The mean concentration of MTX of six groups in brain tissue are followed by A group (1.05±0.26)μmol/L, B group (0.61±0.25)μmol/L, C group (0.48±0.25)μmol/L, D group (2.07±0.35)μmol/L, E group (1.27±0.21)μmol/L, F group (0.59±0.69)μmol/L. Compared the mean concentration of MTX of each group in brain tissue there are statistical significance (P<0.05). MTX concentration in blood and in brain tissue of correlation coefficient followed by 0.82, 0.75, 0.19, 0.81, 0.55, 0.43. Conclusion The chemotherapy acute lymphoblastic leukemia mice of HDMTX scheme, the peak of blood concentration and brain tissue drug concentration is come after injected MTX 0.5 hour, MTX 5 g/m~2 is better permeation blood-brain barrier and more easy make brain tissue drug concentration to reach effectively therapeutic concentration than MTX 3 g/m~2.

Objective To explore the effects of matrine on apoptosis and nuclear factor(NF)-κBp65 activity of HL-60 cells induced by pirarubicin (THP).Methods Effects of the apoptosis:the HL-60 cells in blank control group were cultured 14 hours with RPMI 1640; groups of different concentration drugs:matrine:0.1 μmol/L,1.0 μmol/L,10.0 μmoL/L and THP:1.0 μmol/L,10.0 μmol/L,100.0 μmol/L.The apoptosis of HL-60 cells were tested by fluorescence double colour painting and flow cytometry (FCM).The NF-κBp65 activity of HL-60 cells were determined by FCM.Results The rates of apoptosis of HL-60 cells were (7.14 ± 2.95) ％,(12.34 ± 2.55) ％,(19.3 ±2.31)％ and (31.78 ±4.31)％,(47.25 ±5.27)％,(56.49 ±1.59)％ in matrine 0.1 μmol/L,1.0 μmol/L,10.0 μmol/L and THP 1.0 μmoL/L,10.0 μmol/L,100.0 μmol/L groups,compared to blank control group (6.46 ± 1.45) ％,there were significant difference (F =257.72,677.19 ; all P ＜ 0.001) ; (70.17 ± 5.68) ％ in matrine 0.1 μ mol/L +THP 1.0 μ moL/L group[vs the THP 1.0 μmoL/L group (31.78 ±4.31)％,(t =38.94,P＜0.001)].The rates of NF-κBp65 activity of HL-60 cells in matrine 0.1 μmol/L,1.0 μmol/L,10.0 μmol/L and THP 1.0 μmol/L,10.0 μmol/L,100.0 μmol/L groups were(8.34 ± 1.52)％,(7.11 ± 1.29)％,(4.78 ±0.31)％ and (16.21 ± 1.20) ％,(23.98 ± 3.21) ％,(32.44 ± 2.89) ％,with the control group (8.44 ± 2.20) ％,there were significant difference(F =65.35,P ＜ 0.001 ; F =674.11,P ＜ 0.001) ; (12.01 ± 2.27) ％ in matrine 0.1 μmol/L + THP 1.0 μmol/L group [with (16.21 ± 1.20) ％ of THP 1.0 μmol/L group(t =21.42,P ＜ 0.001)].Conclusions The apoptosis of HL-60 cell is induced by matrine and pirarubicin in a dose-dependent manner.NF-κBp65 activity of HL-60 cell is inhibited by matrine and increased by pirarubicin in a dose-dependent manner.Matrine can enhannce the effect of induction by pirarubicin of apoptosis of HL-60 cell,and decrease activity of NF-κBp65 by pirarubicin.

Objective To investigate the relationship between the paired like homeobox 2B(PHOX2B)protein and N-MYC oncogene(MYCN gene)status with the clinicopathological features and prognosis of pe-ripheral neuroblastic tumors(pNT).Methods The PHOX2B protein expression in pNT tissues of 41 children cases of pNT was detected by immunohistochemical staining,the MYCN gene expression status was detected by fluorescence in situ hybridization technique,and their relationship with clinicopathological features and prognosis was analyzed by continuous correction chi-square test and Kaplan-Meier method.Results The im-munohistochemical staining showed that PHOX2B protein was mainly localized in the cell nucleus,and the positive expression rate in pNT tissue was 82.9％.Its expression level was related to the onset age,differenti-ation degree and prognosis of pNT(P＜0.05),and the intensity of PHOX2B protein expression was not relat-ed to the MYCN gene status(P＞0.05).The survival stage in the children patients with MYCN gene amplification was shorter than that in the children patients with no MYCN gene amplification(P＜0.05).Conclusion PHOX2B protein has the diagnostic significance for pNT and could be used as a reliable diagnostic marker for pNT;MY-CN gene status is related to the survival period in the children patients,which can help to judge the prognosis of pNT children patients.

From December 2022 to January 2023, 4 lung transplant recipients (3 males and 1 female, aged 52-60 years, all received transplantation less than 1 year) were hospitalized in the Department of Thoracic Surgery of the First Affiliated Hospital of Xi'an Jiaotong University due to COVID-19 after surgery. The clinical manifestations were mostly characterized by elevated body temperature accompanied by shortness of breath, and indicators such as heart rate, oxygen saturation, and oxygenation index could reflect the severity of the condition. The therapy was timely adjusted to immunosuppressive drugs, upgraded oxygen therapy, anti-bacterial and anti-fungal therapy, prone ventilation, general treatment, and anticoagulant therapy, depending on the situation. Finally, 3 patients were cured and discharged from hospital, and 1 died.