1.Diagnosis and Management of Partial ACL Tears
Jiwu CHEN ; Shiyi CHEN ;
Chinese Journal of Sports Medicine 1983;0(03):-
Objective To study the maneuver of diagnosis and management of anterior cruciate ligament (ACL) partial tears. Methods 24 cases of ACL partial tears confirmed by arthroscopy including anteromedial band (AMB) tear in 7 cases and posterolateral bulk (PLB) tear in 17 cases were investigated, from Mar 2000 to Jun 2002. ACL reconstruction was performed in 16 cases under arthroscopy. Clinical findings including symptoms or signs, physical examinations and MRI were collected for differential diagnosis of AMB and PLB tear. The efficiency of ACL reconstruction was evaluated by Lysholm scale with average follow-up of 11 months. Results 28.5% AMB and 98.2% PLB tear cases showed knee instability. Regarding the results of anterior drawer test, Lachman test and pivot shift test, it is positive 71.4%, 14.3% and 0 in AMB tears respectively; and 17.6%, 98.2%, 76.5% in PLB tears. The Lysholm scores were significantly increased from preoperative 63.53?8.11 to postoperative 93.47?2.62(P
2.Clinical problems of structural failure after rotator cuff repairs
Yuzhou CHEN ; Jiwu CHEN ; Shiyi CHEN
Chinese Journal of Orthopaedics 2017;37(3):173-182
Arthroscopic rotator cuff repairs are preformed due to growing knowledge of rotator cuff tears among surgeons as well as patients.However,it is unavoidable and may sometimes very common that some patients develop structural failures or retears after surgical repairs because of unique blood supply and healing procedure of the rotator cuff,on which there is still much debate focusing on problems that one needs to deal with in clinical practice.Retears often occur,if without new trauma,during early postoperative period,or more specifically within the first 6 months after repair and are more frequently observed in those with old ages,diabetes or osteoporosis.In addition,risk factors of retears consist of longer preoperative duration of symptoms,larger primitive tear sizes,higher grades of fatty infiltration of muscles on preoperative imaging examinations,excessive tension within reconstructed structures,deteriorated quality of rotator cuff tissues and special shapes of the acromion,etc.Diagnosis is recently based on results of postoperative magnetic resonance imaging or echogram examination.MR/CT arthrography is also used.Each instrument has its advantages and limitations.However,the lack of a golden standard when establishing a diagnosis of rotator cuff retears makes integrating evaluations and comparisons of different methods a difficult question.It has been reported by many that the onset of retear leads to obvious shoulder pain or weakness,whereas more than a few studies demonstrated that very limited influences of retears in spite of generally unaffected shoulder functions,like decreased muscle strength on certain motions.The disagreement is possibly caused by specific methods of diagnosis,function instruments and time point of assessments used by different researchers as studies have displayed that feelings of patients after surgery and results attained by objective examinations are not always parallel.Moreover,some long-time follow-ups studies indicated that the impact posted by retears on shoulder functions evolves over a period of time.Owing to the varied opinions on actual symptoms and final functional outcomes caused by structural failure,the mainstay of treatment for rotator cuff tears are still conservative protocols,including observation and symptoms relief instead of second surgery.Second surgery could be an effective choice for certain patients,especially for those who have apparent symptoms.
3.Research of the Clinical Anatomy of the Medial Patellar Retinaculum
Jiwu CHEN ; Shiyi CHEN ; Peng ZHANG
Chinese Journal of Sports Medicine 2003;0(05):-
Objectives To study the anatomic characteristic of medial patellar retinaculum and its function in patellar stability. Methods Anatomic dissection of the medial patellar retinaculum was performed on 26 knees of 13 embalmed cadaveric specimens,and its composition and conducted functional studies of the several parts were observed respectively. Results The medial patellar retinaculum comprises patellofemoral ligament, patellotibial ligament and patellomeniscal ligament. The medial patellofemoral ligament which is the thickest part in the medial patellar retinaculum contributes to the major restraints to the lateral patellar displacement.
4.Construction of Eukaryotic Expression Plasmid of hIGF-1 Gene and its Expression in Myoblast Cells
Peng ZHANG ; Shiyi CHEN ; Jiwu CHEN
Chinese Journal of Sports Medicine 1982;0(01):-
Objective To construct a eukaryotic expression vector of huma n insuli n-like growth factor-1 (hIGF-1) gene,and to investigate the transfection and ex pression of pcDNA3.1(-)/hIGF-1 eukaryotic expression plasmid in myoblast cells. Methods pcDNA3.1(-)/hIGF-1 eukaryotic expression plasmid was co nstructed. Myobl ast C2C12 cells were transiently transfected with lipofectamine. RT-PCR a nd immunohistochemical methods were used to detect the expression of hIGF-1 gene . MTT test was used to detect the biological activity of the conditioned medium after the transfection. Results There were significant increas es of hIGF-1 mRN A and protein in the myoblast cells transfected with pcDNA3.1(-)/hIGF-1. The con ditioned medium after the transfection showed the biological activity which coul d stimula te the proliferation of fibroblast cells. Conclusion The pcDNA 3.1(-)/hIGF-1,a eukaryotic expression plasmid for hIGF-1 gene is constructed. High levels of hI GF-1 mRNA and protein expression can be obtained in the myoblast cells transfect ed with pcDNA3.1(-)/hIGF-1 eukaryotic expression plasmid. The expressed protein has the biological activity.
5.Levels of heme oxygenase-1 and carbon monoxide and its significance in the Mycoplasma pneumoniae infection in children
Xiaozhen CHEN ; Jiwu WANG ; Chunzhi HE
Chongqing Medicine 2017;46(5):626-627
Objective To explore the heme oxygenase-1 (HO-1) content of carbon monoxide (CO) levels and clinical significance of Mycoplasma pneumoniae infection in children.Methods 219 cases of mycoplasma pneumonia from October 2013 to June 2015 in our hospital were divided into two groups,which were non-asthmatic group (156 cases) and wheezing group (63 cases),and the wheezing group were divided into two groups according to the presence or absence of hypoxemia and mild asthmatic group (39 cases) and severe asthmatic group (24 cases).HO-1 content in the blood was measured by enzyme-linked immunosorbent assay,using dual-wavelength spectrophotometric determination of hemoglobin oxygen percentage of broken (COHb%),analysis of the differences between the groups.Results Children with blood COHb content wheezing group were (2.59 ± 0.40)% and HO-1 levels were (1 813.24 ± 28.34)ng/L and non-respite group were significantly increased,it had significant differences between the two groups (P< 0.05).Group of children with severe wheezing COHb content were (3.63 ± 0.45)% and HO-1 levels were (2 594.34±23.94)ng/L and mild wheezing group were significantly increased,it had significant differences between the two groups (P<0.05).Positive correlation between blood COHb content and HO-1 levels were significantly (r=0.733,P<0.05).Conclusion Mycoplasma pneumoniae infection in children with wheezing blood COHb content and HO-1 levels were significantly increased,and with wheezing exacerbation and showed an increasing trend,suggesting that CO and HO-1 may be involved in Mycoplasma pneumoniae infection in children in the pathogenesis of wheezing,should lead to clinical attention.
6.Expression of transforming growth factor-β and metalloproteinases in joint capsule of frozen shoulder
Hongyun LI ; Shiyi CHEN ; Weitao ZHAI ; Jiwu CHEN
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(11):1363-1366
Objective To explore the role of transforming growth factor (TFG) -β and matrix metalloproteinases( MMPs) in the development of frozen shoulder. Methods Twenty-four patients who underwent shoulder arthroscopy were included, and were divided into frozen shoulder group (n = 12) and control group ( n = 12; n = 2 for shoulder instability, n = 5 for rotator cuff tear and n = 5 for subacromial impingement) . Joint capsule tissues at the rotator cuff interval were obtained, and the expression of TGF-β, MMP-1, MMP-2, MMP-3, MMP-9 and MMP-12 mRNA and protein was detected by Real-time PCR and Western blotting, respectively. Results The expression of TGF-p mRNA in frozen shoulder group and control group was 3.36 × 10~4 ±2.18 × 10~3 and 1.85 × 10~4 ±3.31×10~3, respectively, the expression of TGF-p protein was 1.55 ± 0.33 and 1.13 ±0.21, respectively, and there were significant differences between these two groups (P < 0.05). The expression of MMP1, MMP2, MMP3, MMP9 and MMP12 mRNA and protein in frozen shoulder group was significantly higher than that in control group(P <0.05) . Conclusion The higher expression of TGF-β and MMPs in joint capsule of frozen shoulder may be associated with the development of frozen shoulder.
7.Survival and hIGF-1 Expression of Transplanted Myoblast Carrying hIGF-1 in vivo
Hongyun LI ; Shiyi CHEN ; Jiwu CHEN ; Hongtu WEI ; Peng ZHANG
Chinese Journal of Sports Medicine 1982;0(02):-
Objective To observe the survival of myoblasts carrying hIGF-1 gene transplanted into the mice, and the expression of hIGF-1 in the transplanted mice. Methods Eighty four male C3H mice(20~30g,7~11w)were divided into four groups: group A, B, C and D (20 mice each group), and the remaining four mice were used as normal control. At the middle of the right gastrocnemius muscle, the mice in group A and B were injected with 1?10~6 myoblasts either carried with or without hIGF-1 gene. Muscle contusion at the middle of the right gastrocnemius muscle of the mice in group C and D was produced. At day 3 following injury, they were injected with 1?10~6 myoblasts either carried with hIGF-1 gene (group C) or without hIGF-1 gene (group D). At the day 2, 5, 10, 20, 30 after injection, four mice of each group were sacrificed randomly. BrdU staining in all mice were performed to evaluate cells surviving, and the expression level of hIGF-1 in group A and C was assessed by immunohistochemical staining and real-time-PCR. Results The BrdU staining in both normal and injured mice transplanted with myoblast carried with or without hIGF-1 were positive. hIGF-1 was expressed and secreted in both group A and C. Conclusion The myoblast carrying hIGF-1 gene transplanted into normal or injured mice can survive for a certain period of time, and can secrete hIGF-1.
8.Comparison of the Suspensory and Double Cross-pin Graft Fixation after ACL Reconstruction Using Hamstring Autograft
Hongyun LI ; Shiyi CHEN ; Jiwu CHEN ; Yunxia LI ; Yinhui HUA
Chinese Journal of Sports Medicine 2010;(1):22-25,7
Objective To compare the clinical results of Endobutton and Rigidfix graft fixation at the femoral tunnel after ACL reconstruction with hamstring autografts.Methods 48 patients accepting ACL reconstruction with hamstring autografts were retrospective studied.According to different fixation adopted at the femoral tunnel,they were divided into group A(24 patients,with Rigidfix cross pin)and group B(24 patients,with Endobuaon).All Cases were fixed with Intrafix interference screw and Spiked Washer screw at the tibial tunnel.The average follow-up time was 27 months.KT-1000 arthrometric measurement and the IKDC,Lysholm,Tegner scores were used for evaluation.The diameter of bone tunnel was also measured by MRI.Results Tunnel widening were found in both groups,but the increase of both the femoral and tibial tunnels in group B were significantly greater than group(P<0.05).In both groups after operation,there was significantly increases of IKDC suhjective scores,Lysholm scores,Tegner scores and KT-1000 results also had obviously improvement(P<0.05).But no significant difference was found between two groups after operation.Conclusion Compared with double cross-pins for fixation at the femoral tunnel,tunnel widening was more prone to happen when using Endobutton suspensory fixation system.
9.Effects of Chinese Medicine HUOXUESHENGJI on the Oxidative Stress in Rat Skeletal Muscles after Acute Contusion
Maolin WANG ; Shiyi CHEN ; Yunxia LI ; Jiwu CHEN ; Hongyu LI
Chinese Journal of Sports Medicine 1983;0(03):-
Objective To investigate the effects of Chinese medicine HUOXUESHENGJI(promoting blood flow and tissue regeneration) on the oxidative stress in rat skeletal muscles after acute contusion.Methods Seventy two male SD rats were randomly and equally divided into three groups:(1)Chinese medicine HUOXUESHENGJI group(A),(2)physiological saline group(B),(3)natural healing group(C),and(4)blank control group(D).The muscle contusion was produced at the gastrocnemius by specific device;Local injection at the injured site with Chinese medicine HUOXUESHENGJI in group A;local injection with physiological saline instead of HUOXUESHENGJI in group B,and natural healing group served as control.The muscles were sampled at day 1,4,7 and 14 respectively after contusion,and then the MDA and the activities of antioxidant enzymes(CAT,SOD and GSH-px)in gastrocnemius were investigated.Results As compared with the group B and group C,SOD and GSH-px activities in group A were significantly increased after contusion(P0.05).The con-centrations of MDA in group A were lower than that in group B and group C at day 4 and day 7 after contusion(P
10.Comparison of the Suspensory and Double Cross-pin Graft Fixation after ACL Reconstruction Using Hamstring Autograft
Hongyun LI ; Shiyi CHEN ; Jiwu CHEN ; Yunxia LI
Chinese Journal of Sports Medicine 1982;0(01):-
Objective To compare the clinical results of Endobutton and Rigidfix graft fixation at the femoral tunnel after ACL reconstruction with hamstring autografts.Methods 48 patients accepting ACL reconstruction with hamstring autografts were retrospective studied.According to different fixation adopted at the femoral tunnel,they were divided into group A(24 patients,with Rigidfix cross pin)and group B(24 patients,with Endobutton).All cases were fixed with Intrafix interference screw and Spiked Washer screw at the tibial tunnel.The average follow-up time was 27 months.KT-1000 arthrometric measurement and the IKDC,Lysholm,Tegner scores were used for evaluation.The diameter of bone tunnel was also measured by MRI.Results Tunnel widening were found in both groups,but the increase of both the femoral and tibial tunnels in group B were significantly greater than group(P