Objective To establish a method of HPLC for determination of salidroside in Jintianquban Tablets. Methods Waters C18 column was used. The methanol-water was used as the mobile phase. The flow rate was 0.8 mL/min. The detection wavelength was set at 276 nm. Results For salidroside, the linear range was 0.3 456~3.456 ?g, and the average recovery rate was 100.3% with RSD=1.71%. Conclusion The method is simple, reliable, accurate and can be applied to the quality control of the preparation.