BACKGROUND:The differentiation of neural stem cells (NSCs) is a hot research. Whether brain-derived neurotrophic factors (BDNF) can induce the differentiation of NSCs into neurons has not been detailed studied. OBJECTIVE:To investigate the effect of different concentrations of BDNF on the differentiation of NSCs from adult rat hippocampus into neurons in the medium with epidermal growth factor. DESIGN:A controlled study. SETTING:Department of Human Anatomy, China Medical University. MATERIALS:The experiment was performed at the Neurotomia Laboratory of China Medical University in August 2007. NSCs isolated from hippocampus of adult SD rats were inoculated in a serum-free medium. Three clean adult SD rats (200-250 g each) were provided by Laboratory Animal Department of China Medical University. Dispositions to the rats were consistent with ethical standards of animals. Epidermal growth factor (EGF) and BDNF were bought from R&D Company. METHODS:①NSCs obtained from rat hippocampus were cultured in the serum-free medium containing basic fibroblast growth factor (bFGF), EGF and B27. ②Single cell clone was obtained via limited dilution for the fourth passage of cells. Identification of NSCs for the passage of cells from monoclonal spheres was performed by Nestin immunocytochemistry. Neurons and astroglial cells were identified by immunocytochemistry for neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) a week after differentiation. ③Differentiation for the monoclonal spheres was done to appraisal the proportion of neurons. NSCs were divided for several groups containing 0 μg/L, 50 μg/L, 100 μg/L, 150 μg/L, and 200 μg/L groups according to the doses of BDNF. EGF was added in the media of each group. Immunocytochemistry for NSE was done a week later to count the positive cells. MAIN OUTCOME MEASURES:Proportion of neurons differentiated from NSCs.RESULTS:①NSCs immunocytochemical staining showed that Nestin was positive in monoclonal spheres, and NSE and GFAP were positive in differentiated cells. ②The proportion of differentiation from NSCs into neurons was significantly higher in groups treated with 50 μg/L and 100 μg/L BDNF compared to other groups（t=2.502-5.025, P < 0.05）, but there was no significant difference between 50 μg/L and 100 μg/L BDNF groups（P > 0.05）. Moreover, no significant difference was detected among 0 μg/L, 150 μg/L and 200 μg/L BDNF groups (P > 0.05). CONCLUSION:The optimal concentration of BDNF is 50 μg/L for differentiation from NSCs into neurons while the concentration of EGF is 20 μg/L.

Objective:To investigate the relationship between fester capacity and clinical efficacy on allergic asthma treated by scarring moxibustion.Methods:The patients were randomly divided into scarring moxibustion 9-cone group,3-cone group and 6-cone group.The biggest area,scab-lost time,first-festering time and scarring time of moxibustion sore,and the clinical efficacy in each group was observed.Results:The biggest post-moxibustion sore area in 9-cone group was larger than that in 3-cone group,but had no difference in comparison with 6-cone group;the scab-lost time,first-festering time and scarring time of post-moxibustion sore in each group were of no difference.The clinical efficacy was better in 9-cone group than in 3-cone group,but had no difference in comparison with the 6-cone group.Conclusion:Fester capacity in treating allergic asthma by scarring moxibustion is related to the clinical efficacy and definite fester capacity is the key to good results on allergic asthma.

Objective:To investigate the clinical significance of epidermal growth factor receptor(EGFR),proliferating cell nuclear antigen(PCNA),laminin(LN)and type IV collagen expression in salivary adenoid cystic carcinoma(SACC).Methods:EGFR gene in 78 cases of SACC with complete clinical data was detected by fluorescence in situ hybridization(FISH)technique,the expression of EGFR,PCNA,LN and type IV collagen protein was detected by immunohistochemistry technique(IHC),their correlation with the clin-icopathological parameters was analysed by SPSS 13.00 software.Results:EGFR gene amplification levels(69.2%)was positively related to the ratio of EGFR protein positive expression(7 1 .8%),the expression of EGFR,PCNA,LN and type IV collagen was posi-tively related to the clinical pathological parameters(P<0.05).There was a positive correlation between EGFR and PCNA expression (P<0.05),a negative correlation between LN protein and type IV collagen protein expression(P<0.05).Conclusion:EGFR gene is amplified in SACC.EGFR,PCNA,LN and type IV collagen take part in the occurrence and development of SACC.

Objective:To explore the research hotspots and evolutionary trends in the field of wound repair mechanism research.Methods:This study was a bibliometric analysis study. English literature related to wound repair mechanism published in the core collection of Web of Science database from the establishment of the database to December 26 th, 2023 that met the inclusion criteria were retrieved. The annual number of publications and their citations were counted, and the change trend was analyzed. Based on the aforementioned annual publication volume, the relevant literature in the core collection of Web of Science database in this field from the first 5 years when the publication growth in this field was rapidly turning, to December 31 st, 2023 was searched again, and the total number of publications was recorded and annual growth rate of published literature was calculated; and based on the trend line of annual publication volume, the publication volume in this field in 2024 was predicted. The CiteSpace 6.2.R4 software was used for visualized analysis of the literature from the second retrieval, including the source journals, the cited literature, and the keywords, to discuss the current research status and the evolution of hotspots of wound repair mechanism. Results:The first search retrieved a total of 3 992 literature related to the research on wound repair mechanisms, among which the annual number of publications and their citations increased rapidly from 2015 to 2023. The time limit for the second retrieval was set to be from January 1 st, 2011 to December 31 st, 2023, during which a total of 3 206 literature was published, with an average annual growth rate of 13.30%. According to the publication trend line at this stage, it was predicted that the number of publications in this field will reach 500 in 2024. The literature from the second retrieval was published in 717 journals. The research directions of the top 10 journals with the most published literature (accounting for 18.75% (601/3 206) of the total number of publications) mainly focused on trauma, molecules, pharmacology of Chinese medicine, and stem cells, with the United Kingdom and the United States, etc. as the main publishing countries. There were 7 journals with impact factors >5 and 6 journals belonging to the Q1 or Q2 areas of the Chinese Academy of Sciences. There were 906 nodes and 9 large clusters for the keywords of cited literature of literature from the second retrieval (Q=0.64, S=0.82). The main clusters of cited literature of literature from the second retrieval were #2 matrix metalloproteinases and #3 transforming growth factor β 1 from 2006 to 2015, #1 macrophage polarization, #4 mesenchymal stem cells, #6 antibacterial, and #7 plant extraction from 2016 to 2023. During 2021-2023, the main clusters of cited literature of literature from the second retrieval being #1 macrophage polarization, #4 mesenchymal stem cells, #6 antibacterial, and #7 plant extraction had the most closely related co-occurrence. The analysis of the top 5 cited literature of literature from the second retrieval with high citation value, high centrality value, and high Sigma value showed that the main research directions were the influence of macrophages and inflammation regulation on wound repair, the influence of fibroblasts on wound repair, and the influence of growth factors and cytokines on wound repair. The keywords of literature from the second retrieval formed 636 nodes and 7 clusters, that being #0 antibacterial, #1 mesenchymal stem cells, #2 cell migration, #3 wound repair, #4 exosomes, #5 negative pressure wound treatment, and #6 diabetic foot ulcer (Q=0.59, S=0.80). For the literature from the second retrieval, the main clusters from 2016 to 2023 were #0 antibacterial, #1 mesenchymal stem cells, and #4 exosomes, and the main clusters before 2015 were #2 cell migration and #3 wound repair. A total of 110 burst keywords (hereinafter referred to as burst words) were formed for the keywords of literature from the second retrieval, and the top 10 burst words in terms of intensity were mouse, gene expression, skin injury, epithelial cells, signaling pathways, biomaterials, exosomes, molecular docking, hydrogels, and macrophage polarization, with different start and end time periods. Among them, the high-intensity burst words from 2021 to 2023 were hydrogel (belonging to cluster #0 antibacterial), exosome (belonging to cluster #1 mesenchymal stem cells), molecular docking (belonging to cluster #0 antibacterial), and macrophage polarization (belonging to cluster #0 antibacterial). Conclusions:In the future, the development of wound repair mechanism research will still be at a steady phase. The research hotspots in this field have shifted from growth factors and wound repair physiology to antibacterial and stem cells. Future research directions in this field may include using molecular docking technology and network pharmacology to screen drugs that promote wound repair and study their underlying mechanisms, the regulation of macrophage polarization by exosomes, and the mechanism by which hydrogels promote wound healing through antibacterial effects.

Objective:To construct a value evaluation framework for high-value medical consumables,providing a guidance for medical insurance access and hospital access management scenarios in China.Methods:It conducted literature review,qualitative in-terviews and quantitative surveys.A total of 12 experts were invited for qualitative interviews,while 100 experts from four fields of health technology assessment,medical insurance,hospital management,and clinical practice participated in the quantitative survey.Through those process,it generated the composition of the value framework and the scoring of each item.Differences in ratings be-tween different scenarios and experts were analyzed through chi-square tests.The recommendation level for each item was graded.Re-sults:A comprehensive value evaluation framework for high-value medical consumables was established,which included 6 core dimen-sions,comprised 16 items for secondary dimensions and 50 items for tertiary dimensions.It showed significant differences between the medical insurance access and hospital access scenarios,as well as among different fields of experts in the same scenario.furthermore,grading the items in two scenarios.The medical insurance access scenario had 8 highly recommended items,and the hospital access scenario had 24 highly recommended items.Conclusion:Value evaluation should encourage multi-dimensional assessments and inter-disciplinary participation,continually improving the management of high-value medical consumables in medical insurance and hospital access.