Adult ; Delirium ; chemically induced ; Female ; Humans ; Thiocarbamates ; poisoning

Objective To develop the security scale of college students' sense to investigate college students' subjective feeling of security.According to the characteristics of the feeling tendentiousness to analysis the subject and object factors influencing the insecurity of college students.Methods The security scale of college students' sense compiled was administrated to 500 college student randomly stratified cluster sampling from four universities in Yantai,then description and analysis were analyzed by SPSS17.0,AHP and SAS9.1 to statistical.Results Through the scale of exploratory factor analysis,the Cronbach' s of the scale was 0.629.College students in four dimensions of security and total security were in above-average levels,college students' total scorc of security is significance of difference in grade,gender and whether come from the single parent(all P＜0.05) ;Self security was significance of difference in the grade and the gender(P＜0.01 and P＜0.05) ; and interpersonal security in gender and whether come from the single parent had significance difference(P＜0.05) ;Determine control and family security in these demographic variable aspects had no significant differences.Multivariate factors analysis results showed that the decision tree model was better than the logistic model (the misclassification rate of the model was lower than the decision tree model).The main factors affecting the university students' security were gender,grade,the average income and whether they,were the only children.The misclassification rate of the decision tree model was lower than the model of logistic.College students' sense of dimensions are significant with positive correlation,the dimension of the security level may affect other security of the situation.Conclusion The security scale of college students' sense has good reliability and validity and can be used in the study of college security.The factor analysis determines it can be used multiple factors analysis.The survey shows that the overall security of the crowd' s is over the average level.The gender,grade,annual per-capita income and whether the one-child are the potential factors that affect college students' insecurity.

Humans ; Incisor ; Tooth Apex ; abnormalities

Objective To study the effect of fund support on cited times of Chinese papers on AIDS by controlling the influence of confounding factors.Methods The effect of publication time of fund-supported papers and non fund-supported papers, h-index of authors, IF of journals on accumulated cited times of each academic paper was controlled by propensity score matching.The cited times of matched fund-supported papers and non fund-supported papers were compared by paired t test.Results The balance of confounding factors which were unbalanced before matching was achieved between fund-supported papers and non fund-supported papers after matching.No significant difference was found between fund-supported papers and non fund-supported papers after matching.Conclusion Fund support does not affect the cited times of Chinese papers on AIDS, which shows that fund support can not noticeably improve the academic level and impact of Chinese papers on AIDS.

Objective To evaluate the relationship between Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling pathway and propofol-induced inhibition of endotoxin-induced release of tumor necrosis factor-alpha (TNF-α) from alveolar macrophages (AMs) of rats.Methods AMs extracted from adult male Sprague-Dawley rats were cultured and inoculated in 6-well plates (1 × 106 cells/well)and in 96-well plates (1×104 cells/well).The cells were divided into 5 groups (n=18 each) using a random number table:control group (group C),dimethyl sulfoxide group (group D),lipopolysaccharide (LPS) group (group L),propofol group (group P) and LPS plus propofol group (group L+P).The cells were continuously cultured with phosphate buffer solution in group C.Dimethyl sulfoxide was added at the final concentration of 5 mg/ml in group D.LPS was added at the final concentration of 1 μg/ml in group L.Propofol was added at the final concentration of 25 μmol/L (4.46 μg/ml) in group P.LPS and propofol were added at the final concentration of 1 μg/ml and 25 μmol/L (4.46 μg/ml),respectively,in group L+P.At 24 h of culture or incubation,the cell viability was detected by CCK-8 assay,the morphological changes of cells were observed using Wright's staining,the concentration of TNF-α in the supernatant was determined by enzyme-linked immunosorbent assay,and TLR4 expression and NF-κB activities were measured by Western blot.Results Compared with group C,the cell viability and concentration of TNF-α in the supernatant were significantly increased,the expression of TLR4 was up-regulated,and the activity of NF-κB was enhanced in L and L+P groups (P＜0.05),and no significant change was found in the parameters mentioned above in D and P groups (P＞0.05).Compared with group L,the cell viability and concentration of TNF-α in the supernatant were significantly decreased,the expression of TLR4 was down-regulated,and the activity of NF-κB was weakened (P＜0.05),the morphological changes of cells were significantly attenuated,and the number of pseudopodia was reduced in group L+P.Conclusion The mechanism by which propofol inhibits endotoxin-induced release of TNF-α from AMs is related to inhibited activation of TLR4/NF-λB signaling pathway in rats.

Changes in behaviour relevant to the vestibular system were studied in Long-Evans rats which were fertilized, born and housed in 2 acceleration of gravity for 4 months and thereafter exposed to 1 acceleration of gravity, and expression of Fos protein in the brain stem was examined. Data from the hypergravity rats were compared respectively with those from the rotation group and the labyrinthectomized group. Static and locomotion modes of the hypergravity rats were changed, tension of extensor was enhanced and the abilities in locomotion equalization and orientation in swimming and air-righting response were reduced. The adaptation process varied with different behaviours. The time for recovery of the ability of orientating in swimming was the longest, taking more than 1 month. The Fos protein expression provides a useful tool for mapping brain functional activities after sensory stimulation, showing a low basal level in normal and labyrinthectomized groups. The hypergravity rats, on the other hand, exhibited more Fos-positive cells in the superior colliculus, inferior colliculus, periaqueductal gray, raphe dorsal nucleus and solitary nucleus. In contrast, the inferior olivary nuclei, locus coeruleus and vestibular nuclei were not strongly labeled. These spatial patterns of Fos expression suggest that a decrease in gravity-inertial force may activate a neural pathway different from the vestibulo-olivar pathways activated by an increase in gravity-inertial force.

The computer-assisted surgery system is a complex system. All of the errors can be attributed to the loss of correspondence between the world coordinate system in the operation room and the virtual world coordinate system obtained from the multi-model medical images. The system's accuracy is composed of the accuracy of the localizer and that of registration. In order to improve the system accuracy, we analyse most of the possible error sources. The accuracy of the localizer affects deeply the registration between the intra-operation and pre-operation data. The localizer is the most basic and important part for a computer-assisted surgery system. We give a comprehensive possible error source at the end of the paper.
Algorithms ; Equipment Design ; Equipment Failure Analysis ; Imaging, Three-Dimensional ; instrumentation ; methods ; Magnetic Resonance Imaging ; Phantoms, Imaging ; Software ; Surgery, Computer-Assisted ; instrumentation ; methods ; Tomography, Emission-Computed, Single-Photon ; Tomography, X-Ray Computed

OBJECTIVETo observe the effect of garlicin on adhension molecules CD11a and deformability of peripheral neutrophil in patients with acute cerebral infarction (ACI).

METHODSNeutrophils were separated from peripheral blood of healthy subjects and ACI patients, and incubated in 37 degrees C in vitro. The CD11a expression was detected by antibody fluorescence labeling method and the time of neutrophils passing millipore membrane were measured for calculation of the filter index.

RESULTSCD11a expression rate in healthy subjects was 34.64 +/- 25.34%, while in patients was 55.35 +/- 30.54%, difference between them was significant (P < 0.05). After garlicin treatment, it lowered to 49.16 +/- 31.68%, as compared with untreated group, P < 0.05. The neutrophil filter index in healthy group, untreated group, garlicin treated group and Nimodipine treated group was 0.87 +/- 0.46, 6.42 +/- 6.40, 3.47 +/- 3.67 and 5.03 +/- 3.72 respectively, comparison between that in the garlicin treated group and in untreated group showed significant difference (P < 0.05).

CONCLUSIONGarlicin could effectively inhibit the CD11a expression in peripheral blood neutrophils and improve the deformability of the neutrophils in ACI patients.

Aged ; Allyl Compounds ; pharmacology ; CD11a Antigen ; biosynthesis ; Cell Adhesion ; drug effects ; Cell Separation ; Cerebral Infarction ; blood ; Disulfides ; pharmacology ; Erythrocyte Deformability ; drug effects ; Female ; Garlic ; chemistry ; Humans ; Male ; Middle Aged ; Neutrophils ; physiology

The T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT) is an inhibitory receptor mainly expressed on active T-cells, or natural killer cells (NK cells) that activate negative stimulus signals in immune cells by combining with multiple ligands on the surface of target cells including tumor cells and infected cells. TIGIT plays an important regulatory role in the immune pathogenesis of tumors, viral infections and various autoimmune diseases by inhibiting the over activation of cells and the over secretion of proinflammatory cytokines. Recent researches show that TIGIT is highly expressed in T cells and NK cells of cancer patients, and is related to disease progression and poor clinical prognosis. Researchers try to enhance the activity of T cells or NK cells by blocking the binding of TIGIT and its ligand for therapeutic intervention. At present, there have been many reports about the use of anti-TIGIT monoclonal antibody treatment in different mouse tumor models leading to tumor regression, TIGIT has received extensive attention in cancer immunotherapy as a promising target for next generation cancer immunotherapy. Several clinical trials are currently evaluating the efficacy of anti-TIGIT monoclonal antibodies (mAbs) in patients with several cancers. The most advanced candidate, tiragolumab, has exhibited remarkable efficacy in programmed cell death ligand 1 (PD-L1)-positive non-small cell lung carcinoma (NSCLC) patients in phase Ⅱ clinical trials, in combination with PD-L1 blockade. However, the specific mechanism of TIGIT blockade remains to be fully elucidated.

OBJECTIVE: To explore the related risk facts of pulmonary thromboembolism (PTE) and analyze the relation between PTE and the trauma or medical behavior by investigating the cases of PTE. METHODS: Thirty-three cases were selected from Institute of Forensic Science (IFS) from 2000 to 2014. RESULTS: In 33 cases, 16 decedents were male, 17 decedents were female; different degrees of dyspnea, chest tight- ness and syncope symptoms were the clinical manifestation of the deceased; the thrombus was mainly distributed in the left and right pulmonary arteries. The main source of embolism was the deep vein of lower limb and the left probability was higher. Trauma, limited position, operation and cardiovascular disease showed high-risk factors of PTE; D-Dimer test, hemolytic test and computer tomography pul- monary angiography were the diagnostic tools for PTE. In some cases, trauma and medical malpractice could be involved in the cause of death. CONCLUSION Non-typical clinical symptoms present in the most cases caused by PTE, and these cases always show many high-risk factors. The relation between PTE and injury or medical behavior should be considered carefully in the forensic pathological practice.
Female ; Fibrin Fibrinogen Degradation Products/chemistry* ; Forensic Pathology ; Humans ; Lower Extremity/pathology* ; Lung/pathology* ; Male ; Malpractice ; Pulmonary Embolism/mortality*