Objective To observe and analyze the clinical effect of dexmedetomidine combined with sufentanil in the treatment of ICU mechanical ventilation. Methods 80 cases of critically ill patients treated by mechanical ventilation in our hospital ICU from February 2014 to March 2015 were randomly divided into study group and control group (n=40), the study group was treated with dexmedetomidine and sufentanil combined drug treatment, the control group was treated with sufentanil and midazolam combined drug treatment. The sedative effect and analgesic effect of the two groups were compared at different time points after the administration. Results At each time point after medication, there was no significant difference with the score of SAS between study group and control group; The BPS scores of respiratory compliance, upper limb and facial expression of the study group were lower than those of the control group, the difference was statistically significant; the incidence of adverse reaction in the study group was 7.5% (3/40), which was significantly lower than that of the control group 22.5% (9/40), the difference was statistically significant (P<0.05); There were no significant differences in MAP, SpO2 and HR between the two groups before treatment; After treatment, the indexes of MAP, SpO2 and HR in the two groups at different time points and after extubation were significantly different from those before treatment (P<0.05), The difference of MAP, SpO2 and HR between the two groups at each time point after treatment and after extubation were statistically significant (P<0.05). Conclusion The combination of dexmedetomidine and sufentanil for ICU mechanical ventilation has a good sedative and analgesic effect, and is worthy of clinical practice, and it is worthy of clinical practice.

Objective: To investigate the antagonistic effect of lithium on the neurotoxicity of lead acetate and the possible mechanisms. Method: Wistar rats were randomly divided into control group, Pb group and four Pb+ LiCl groups fed with feed containing 3, 30, 300, 3 000 mg/kg LiCl respectively. All Pb exposed groups were given distilled water containing 0.2% PbAc. The changes of nNOS protein and gene expression in rats hippocampus were studied by ABC immunohistochemistry and the semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) method. Rusults: Compared with the control group, the number of nNOS positive neurons in CA1 area and the nNOS mRNA content of hippocampus of LiCl (3, 30 mg/kg) groups were increased,while those of Pb-exposed rats were significantly decreased. Compared with Pb groups, the number of nNOS positive neurons and the nNOS mRNA content of Pb+LiCl (3,30,300 mg/kg) groups were increased. In dentate gyrus, the changes of the number of nNOS positive neurons coincided with the changes in CA1 area, but those in CA3 area showed little difference. Conclusion: Low dose of lithium could resist the lead neurotoxcity obviously.

Objective To evaluate irregular hepatectomy combined with removal of tumor thrombi, postoperative hepatic artery chemotherapy(HAC) and portal vein chemotherapy (PVC) for the treatment of hepatocellular carcinoma (HCC) with portal vein tumor thrombi (PVTT). Methods From Jan. 1987 to Dec. 1996, 62 HCC cases with PVTT underwent the combined therapy. Among them HAC and PVC were given in 40 cases. 59 patients were followed up for over 3 years. Results 6 cases died of postoperative hepatic and renal failure in 3 months. Recurrent rate and survival rate at 1, 2, 3 year was 46%(18/39), 59%(23/39),79%(31/39)and 69%(27/39),51%(20/39), 31%(12/39) respectively in group of combined HAC and PVC compared with 80%(16/20),90%(18/20),100%(20/20)and 30%(6/20),10%(2/20), 5%(1/20)in group without postoperative HAC and PVC. Conclusions Irregular hepatectomy with removal of tumor thrombi is an effective treatment for HCC with PVTT. Postoperative use of HAC and PVC significantly reduces the recurrence rate and improves survival.

As the main drug in colorectal cancer chemotherapy,oxaliplatin is gradually infiltrated into other malignancies therapy.But oxaliplatin-induced peripheral neuropathy limits its clinical application.The mechanisms of oxaliplatin neurotoxicity are not yet clear.Recent researches show that the acute neurotoxicity of oxaliplatin is mediated through changes in Na + transient conductances.And the function of the mitogen-activated protein kinases in chronic neuropathy has already been demonstrated in vitro.Furthermore,numerous indicators can be used to predict oxaliplatin-induced peripheral neuropathy,which bring the hope for improving the continuity of chemotherapy and realizing personalized medicine therapy.

Objective:To study the difference of c-fos expression of neurons in subareas of hippocampus of morphine dependent mice.Methods:Mice were given (sc) increasing doses of morphine to form morphine dependent models and withdrawal syndromes were precipitated by naloxone. The intensity of withdrawal syndromes was evaluted accoding to indices ,such as the number of jumping ,the weight loss,et al .The expression of Fos positive neurons in subareas of CA1, dentate gyrus and CA3 of hippocampus of morphine dependent group was observed by immunohistochemistry assay. Results:The number of Fos positive cells in subareas of CA1 and dentate gyrus of morphine dependent group was much higher than that of the normal control group(P

Objective　To study the molecular mechanism about injurious effect of morphine dependence on the construction and function of hippocampal neurons. Method　 Mice were given (sc) increasing doses of morphine to form morphine-dependence model ,and the DNA and RNA changes stained with acridine orange (AO) fluorescence probe technique were investigated in hippocampal neurons of morphine-dependence group,naloxone-precipitated withdrawal group in morphine-dependence mice and control group. Results　 Compared with control group , the staining changes of DNA,RNA decreased obviously in hippocampal neurons of both morphine-dependence group and naloxone- precipitated withdrawal group in morphine-dependence mice,especially the later. Conclusion　Both morphine-dependence and naloxone-precipitated withdrawal in morphine-dependence mice injured nucleic acid metabolism in hippocampal neurons ,especially the later.Those changes may be some reasons of decreased brain function ,especially in learning and memory deficits.

Objective: To develop Attributional Style Questionnaire for Adolescents (ASQA) and explore the correlation between attributional style and mental health in adolescents. Method: ASQA was drawn up through referring to Peterson's method. 534 students were measured by ASQA, SSDA, SAS and MMOCI. Results: The goodness of fit indices of three-factor model in ASQA as follows: x2/df = 511.69/188, GFI = 0.91, AGFI = 0.89, NNFI = 0.91, CFI = 0.92, IFI = 0.92. Cronbach's α coefficient was 0.82. Spearman - Brown split - half reliability was 0.84. Stable and global variables entered the equations of depression, anxiety, obaession and compulsion in multiple linear regression analysis. Conclusion:ASQA had good reliability and construct validity. Adolescents were in low level of mental health when they attributed negative events to stable and global causes.

Experiment demonstrated that total soyasaponin (TS ) possesed significant inhibitory effect on replication of HSV-1 and CoxB3 viruses and showed marked protective effects on the infected cells (P

Objective:To observe the effects of zinc supplementation on the function of brain after sleep deprivation(SD).Methods:Rats SD model was made by the “Flower Pot" technique.Three days later,the rats received Y-labyrinth test to test the function of learning and memory.NADPH-d histochemistry and ABC immunohistochemistry were applied to exam the activity of NOS and the levels of protein expression of nNOS in hippocampus of rats.Results:Compared with the normal group,Learning times of rats in Y-labyrinth test of the SD group increased(89.3?25.3 /67.1?29.3,t=1.81,P0.05).Compared with the SD group,in TZC supplementation group,the levels of NOS in sub-area of hippocampus(CA_1:27.2?2.8;CA_3:15.3?1.6;DG:21.8?1.9,P0.05).Conclusions:TZC supplementation can improve learning and memory function of rats,and the mechanisms might be related to increased level of NOS and the protein expression of nNOS in hippocampus of animals.

Objective To study the antagonism of taurine-zinc coordination compound (TZC) to the adverse effect of mercury (Hg) on the neurons in the cerebral cortex. Methods 32 male Wistar rats aged 21days were randomly divided into 4 groups, one control group (fed on distilled water), three experimental groups fed on HgCl2 (0.06 g/L), HgCl2+0.23 g/L TZC and HgCl2+0.46 g/L TZC respectively, treated for one month. The NOS activity in the cerebral cortex neurons was determined by NADPH-d histochemistry. Results NADPH-d positive neurons increased in HgCl2 group (P