Objective: To investigate the antagonistic effect of lithium on the neurotoxicity of lead acetate and the possible mechanisms. Method: Wistar rats were randomly divided into control group, Pb group and four Pb+ LiCl groups fed with feed containing 3, 30, 300, 3 000 mg/kg LiCl respectively. All Pb exposed groups were given distilled water containing 0.2% PbAc. The changes of nNOS protein and gene expression in rats hippocampus were studied by ABC immunohistochemistry and the semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) method. Rusults: Compared with the control group, the number of nNOS positive neurons in CA1 area and the nNOS mRNA content of hippocampus of LiCl (3, 30 mg/kg) groups were increased,while those of Pb-exposed rats were significantly decreased. Compared with Pb groups, the number of nNOS positive neurons and the nNOS mRNA content of Pb+LiCl (3,30,300 mg/kg) groups were increased. In dentate gyrus, the changes of the number of nNOS positive neurons coincided with the changes in CA1 area, but those in CA3 area showed little difference. Conclusion: Low dose of lithium could resist the lead neurotoxcity obviously.

Objective To observe and analyze the clinical effect of dexmedetomidine combined with sufentanil in the treatment of ICU mechanical ventilation. Methods 80 cases of critically ill patients treated by mechanical ventilation in our hospital ICU from February 2014 to March 2015 were randomly divided into study group and control group (n=40), the study group was treated with dexmedetomidine and sufentanil combined drug treatment, the control group was treated with sufentanil and midazolam combined drug treatment. The sedative effect and analgesic effect of the two groups were compared at different time points after the administration. Results At each time point after medication, there was no significant difference with the score of SAS between study group and control group; The BPS scores of respiratory compliance, upper limb and facial expression of the study group were lower than those of the control group, the difference was statistically significant; the incidence of adverse reaction in the study group was 7.5% (3/40), which was significantly lower than that of the control group 22.5% (9/40), the difference was statistically significant (P<0.05); There were no significant differences in MAP, SpO2 and HR between the two groups before treatment; After treatment, the indexes of MAP, SpO2 and HR in the two groups at different time points and after extubation were significantly different from those before treatment (P<0.05), The difference of MAP, SpO2 and HR between the two groups at each time point after treatment and after extubation were statistically significant (P<0.05). Conclusion The combination of dexmedetomidine and sufentanil for ICU mechanical ventilation has a good sedative and analgesic effect, and is worthy of clinical practice, and it is worthy of clinical practice.

Objective To evaluate irregular hepatectomy combined with removal of tumor thrombi, postoperative hepatic artery chemotherapy(HAC) and portal vein chemotherapy (PVC) for the treatment of hepatocellular carcinoma (HCC) with portal vein tumor thrombi (PVTT). Methods From Jan. 1987 to Dec. 1996, 62 HCC cases with PVTT underwent the combined therapy. Among them HAC and PVC were given in 40 cases. 59 patients were followed up for over 3 years. Results 6 cases died of postoperative hepatic and renal failure in 3 months. Recurrent rate and survival rate at 1, 2, 3 year was 46%(18/39), 59%(23/39),79%(31/39)and 69%(27/39),51%(20/39), 31%(12/39) respectively in group of combined HAC and PVC compared with 80%(16/20),90%(18/20),100%(20/20)and 30%(6/20),10%(2/20), 5%(1/20)in group without postoperative HAC and PVC. Conclusions Irregular hepatectomy with removal of tumor thrombi is an effective treatment for HCC with PVTT. Postoperative use of HAC and PVC significantly reduces the recurrence rate and improves survival.

As the main drug in colorectal cancer chemotherapy,oxaliplatin is gradually infiltrated into other malignancies therapy.But oxaliplatin-induced peripheral neuropathy limits its clinical application.The mechanisms of oxaliplatin neurotoxicity are not yet clear.Recent researches show that the acute neurotoxicity of oxaliplatin is mediated through changes in Na + transient conductances.And the function of the mitogen-activated protein kinases in chronic neuropathy has already been demonstrated in vitro.Furthermore,numerous indicators can be used to predict oxaliplatin-induced peripheral neuropathy,which bring the hope for improving the continuity of chemotherapy and realizing personalized medicine therapy.

Objective　To study the molecular mechanism about injurious effect of morphine dependence on the construction and function of hippocampal neurons. Method　 Mice were given (sc) increasing doses of morphine to form morphine-dependence model ,and the DNA and RNA changes stained with acridine orange (AO) fluorescence probe technique were investigated in hippocampal neurons of morphine-dependence group,naloxone-precipitated withdrawal group in morphine-dependence mice and control group. Results　 Compared with control group , the staining changes of DNA,RNA decreased obviously in hippocampal neurons of both morphine-dependence group and naloxone- precipitated withdrawal group in morphine-dependence mice,especially the later. Conclusion　Both morphine-dependence and naloxone-precipitated withdrawal in morphine-dependence mice injured nucleic acid metabolism in hippocampal neurons ,especially the later.Those changes may be some reasons of decreased brain function ,especially in learning and memory deficits.

Objective: To develop Attributional Style Questionnaire for Adolescents (ASQA) and explore the correlation between attributional style and mental health in adolescents. Method: ASQA was drawn up through referring to Peterson's method. 534 students were measured by ASQA, SSDA, SAS and MMOCI. Results: The goodness of fit indices of three-factor model in ASQA as follows: x2/df = 511.69/188, GFI = 0.91, AGFI = 0.89, NNFI = 0.91, CFI = 0.92, IFI = 0.92. Cronbach's α coefficient was 0.82. Spearman - Brown split - half reliability was 0.84. Stable and global variables entered the equations of depression, anxiety, obaession and compulsion in multiple linear regression analysis. Conclusion:ASQA had good reliability and construct validity. Adolescents were in low level of mental health when they attributed negative events to stable and global causes.

Objective To study the effect of HCV C gene transfection on expression of hTERT mRNA in human biliary carcinoma cell lines (QBC939) and to elucidate the significance of activation of hTERT mRNA by HCV C gene on the carcinogenesis of bile duct cells. MethodsThe recombinant plasmid(pcDNA3-HCVC) and the vector-alone were co-transfected with enhanced green fluorescent protein( EGFP )into QBC939 and human normal bile duct epithelial cells(HBEC) using liposome. The reverse transcription PCR(RT-PCR) and immunocytochemical stain were used to show the expression of hTERT mRNA and protein. Results The transfection efficiency of pcDNAHCV-C,which was determined by the expression of EGFP,is about 16% and 30% in QBC939 and HBEC respectively. There was no expression of hTERT mRNA assayed in HBECs when transfected blank vector,but a dramatic increase was observed for hTERT mRNA expression in HBEC when transfected with HCV C expressing vector. The expression of hTERT mRNA and protein assayed in QBC939 significantly increased when transfected with HCV C expression vector than that transfected with blank vector. Conclusion HCV C gene transfection up-regulates the transcriptional expression of hTERT mRNA in biliary carcinoma cells,it is suggested that HCV C protein contributes to virus carcinogenesis.

To investigate the effect of Chinese herbs (Astragalus-As,Angelica-An,AG-a composition of Astragalus) on muscle protein metabolism in nephrotic rats. Methods The method of incorporation of [3 H-phenylalanine ] ([3 H-phe]) into the muscle was used. Results (l)The incorporation of [3 H-phe] into the muscle of untreated nephrotic rats (1.12 ? 0.37) was significantly lower than ihe normal control rats (2.25?0.47) ( P

Objective:To study the effect of taurine on protection against lead toxicity in cultured cortex neurons. Methods:Put taurine and different concentrations of lead acetate in cultures of cortex neurons and observe the length of dendites and the growth activity of neurons. Results:At the concentration of 10-8 mol/l lead acetate the growth of cultured cortex neurons were decreased and even the cells died. Taurine at the concentration of 1.6?10-3 mol/L could protect against lead toxicity and promote neuron to grow. Conclusion:Taurine can protect from lead acetate toxicity in cultured cortex neurons.

Objective: To explore the protective effects of zinc on learning and memory of mice suffering from acute repeated hypoxia. Method: 36 mice were divided into three groups randomly: normal control, saline+hypoxia (NS) group, zinc+hypoxia (Zinc) group. Model of acute repeated hypoxic mice was duplicated and the anoxia endurance was recorded. The step-through test and step-down test were used to evaluate the ability of learning and memory. Results: 1.Compared with saline+hypoxia group, the anoxia endurance was significantly increased in zinc+hypoxia group (P