Purpose:To explore the value of predicting the axillary lymph node(ALN) metastases statu s by sentinel lymph node biopsy(SLNB) with methylene blue subcantaneously inject ion in the breast cancer patient and to provide the scientific basis for selecti ve axillary lymph node dissection(ALND) in breast cancer.Methods :1% methylene blue 2ml was subcantaneously injected at 4 sites of the skin over the tumor in each of 64 patients with breast cancer. Most of these were staged as T 1-2 N 0M 0, and a few were T 3N 0M 0. All patients underwent a S LNB 5 minutes after injection, followed by various ways of radical operation. Pa thohistological examination was assessed in all of SLN which included a frozen s ection intra-operation and a paraffin section after operation, and all other no n-SLN had a paraffin section.Results:The successful detection rate of SLN was 85.9%(55/64), and the accuracy of predi cting ALN metastases was 96.4%(53/55). The sensitivity was 90.9%(20/22), the sp ecificity was 100%(33/33), and the false-negative rate was 9.1%(2/22). The pred ictive value of a positive test and of a negative test were respectively 100%(22 /22) and 94.3%(33/35). In the resected SLN and non-SLN the metastases rates wer e respectively 29.4% and 8.3%(? 2=41.493, P

OBJECTIVE:To establish a RP-HPLC method for simultaneously determining 2 components in compound ornidazole suppository.METHODS:The HPLC method was performed on Symmetry C18 column(4.6mm?150mm),a mixture of methanol and 0.01mol/L KH2PO4 as mobile phase,and tinidazole as internal standard.The detection wavelength was set at 305nm,and flow rate was 1.0ml/min.RESULTS:The linear rangs of ornidazole and levofloxacin were 5～80?g/ml(r=0.9 997)and 2.5～40?g/ml(r=0.9 999)respectively.The average recovery(n=5)was (100.65?2.27)%for ornidazole,(98.86?1.28)%for levofloxacin.CONCLUSION:This method is rapid,simple and accurate and can be used for the quality control of compound ornidazole suppository.

OBJECTIVE:To probe the characteristics and regularities of drug-induced allergic shock.METHODS:The case reports of drug-induced allergic shock,published on Chinese medical periodicals during the period of1998to2003,were collected and analysed.RESULTS:Ocurrence rate and mortality of allergic shock induced by antimicrobials,Chinese materia medica and biologicals occupied the front3places in sequence,and drugs for intravenous administration resulted in the highest rate(61.30%).A large proportion of allergic shock was immediate type(78.73%).Drug-induced allergic shock was indepen?dent of sex and age.CONCLUSION:Allergic history should be taken in detail before the use,the indications of drug use strictly controlled and the ADRs observed so as to ensure the safe use of drugs.

OBJECTIVE:To optimize the extraction technology of total saponins from Litchi chinensis.METHODS:The extraction technology of total saponins from Litchi chinensis was optimized by orthogonal experiment taking the content of total saponins and extraction rate as indexes with the extraction solvent,the amount of solvent,the extraction time and extraction times as factors. RESULTS:The optimal extraction technology of total saponins from Litchi chinensis was as follows:70% ethanol as solvent,1 g medicinal powder(per 8 mL solvent),extraction time of 1 hour (extraction for 1 time). CONCLUSION:The optimum extracting technology is simple,feasible and stable,and it provides theoretic basis for the further utilization of Litchi chinensis.

PURPOSE To discuss induction and relation of synchronous multiple primary cancer to adenoma of large intestines. METHODS Continuous observation of resectable and pathologically proved cancer of large intestines of 166 cases. RESULTS We discovered 8 cases (4. 8%) of synchronous multiple primary cancer of large interstines. 43 cases of single primary cancer of concomitant adenoma (27. 7%). The 4 cases of synchronous cancer had merged adenoma (50%). The 4 cases of synchronous cancer had concomitant adenoma of 14 sites. Of these 2 cases had neoplastic change in 3 sites. The rate of neoplastic change was 50% concomitant adenoma. In the whole group, of the 18 cancer sites, 8 nidi were less than 3cm. CONCLUSION Synchronous cancer of large intestines is not rare, and is related to malignant change of adenomas. complete colonoscopy is an important means to improve diagnostic rate.

Objective To observe the effects of parecoxib sodium on laparoscopic cholecystectomy ( LC ) patients after remifentanil fast track anesthesia.Methods 120 patients underwent general anesthesia for laparoscopic cholecystectomy were divided into two groups using random number table,60 cases in each group.The observation group was injected parecoxib sodium 40mg+saline solution 5 mL,while the control group was injected only saline so-lution 5mL prior to induction of anesthesia.The heart rate,mean arterial pressure,awaked time,pain score,agitation score and adverse reaction were compared.Results 5, 15, 30min after extubation, heart rate were ( 75.7 ± 4.8)times/min,(79.3 ±6.2)times/min,(74.2 ±5.2)times/min in the observation group and (85.2 ±3.8)times/min, (88.2 ±5.9)times/min,(86.1 ±6.2)times/min in the control group,and the mean arterial pressure were (103.5 ± 11.3)mmHg,(104.7 ±12.6)mmHg,(108.4 ±12.7)mmHg in the observation group and (132.3 ±16.3)mmHg, (135.2 ±14.6) mmHg,(133.6 ±11.8) mmHg in the control group,there were statistifically significant differences (t=4.722,4.357,4.236,4.732,4.367,4.063,all P <0.05).The pain score,agitation score were (2.5 ± 0.9)points,(1.4 ±0.8) points in the observation group,which were lower than (4.2 ±1.2) points,(3.2 ± 1.2)points in the control group(t =4.232,5.234,all P<0.05).Conclusion Parecoxib sodium can reduce the fast-track anesthesia agitation and pain,improve hemodynamics and stress response in LC patients.

Changes of ventricular relaxation, contractility and compliance and their effects on cardiac pump function during the development of chronic high output heart failure were studied in the animal model produced by establishment of combined aorto-caval fistula (ACF) and unilateral renal artery stenosis (RAS) in rats.In the present study, all the rats with ACF+RAS showed the characteristics of high output hear failure of varying degree, the cardiac index rised significantly even at rest, while pump function reserving force (CORF) decreased to different extent.The resu(?)lts show that with the aggravation of cardiac pump function deterioration, ventricular relaxation and contractility decreased progressively, the ventricular diastolic compliance rised significantly. With stepwise regression analysis, the changes of ventricular relaxation, comtractility and compliance have different effects on cardiac pump function at different stage of heart failure.

Objective To study the clinical value of serum SCC,CYFRA21-1,NSE and CEA for patients with nasopharyngeal carcinoma (NPC) and squamous cell carcinoma of head and neck (NHSCC). Methods From Aug 2004 to Sep 2007, the serum levels of SCC, CYFRA21-1, NSE and CEA were detected in 79 NPC and 51 HNSCC before and after radiotherapy. The clinical related factors were analyzed. Results Before treatment, the positive rates of serum SCC, CYFRA21-1, NSE and CEA for patients with NPC were 10.53 %, 46.75 %, 17.5 % and 2.04 %, respectively. The sensitivity of combined detection was 78.95 %. The positive rates for patients with HNSCC were 22 %, 19.75 %, 11.11% and 0, respectively. The sensitivity of combined detection was 36.17 %. The level of SCC was related to staging in HNSCC group. CYFRA21-1 was related to the rote of treatment response in NPC group. Conclusion The sensitivity of combined detection of tumor markers were significantly increased. CYFRA21-1 associated with NPC and SCC with HNSCC were noted in this study.

Objective To observe and analyze the clinical effect of dexmedetomidine combined with sufentanil in the treatment of ICU mechanical ventilation. Methods 80 cases of critically ill patients treated by mechanical ventilation in our hospital ICU from February 2014 to March 2015 were randomly divided into study group and control group (n=40), the study group was treated with dexmedetomidine and sufentanil combined drug treatment, the control group was treated with sufentanil and midazolam combined drug treatment. The sedative effect and analgesic effect of the two groups were compared at different time points after the administration. Results At each time point after medication, there was no significant difference with the score of SAS between study group and control group; The BPS scores of respiratory compliance, upper limb and facial expression of the study group were lower than those of the control group, the difference was statistically significant; the incidence of adverse reaction in the study group was 7.5% (3/40), which was significantly lower than that of the control group 22.5% (9/40), the difference was statistically significant (P<0.05); There were no significant differences in MAP, SpO2 and HR between the two groups before treatment; After treatment, the indexes of MAP, SpO2 and HR in the two groups at different time points and after extubation were significantly different from those before treatment (P<0.05), The difference of MAP, SpO2 and HR between the two groups at each time point after treatment and after extubation were statistically significant (P<0.05). Conclusion The combination of dexmedetomidine and sufentanil for ICU mechanical ventilation has a good sedative and analgesic effect, and is worthy of clinical practice, and it is worthy of clinical practice.

OBJECTIVE:To systematically review the effectiveness and safety of combination prescription of lidocaine with ropivacaine for anesthesia,and provide evidence-based reference for developing new compound preparation. METHODS:Retrieved from CJFD,Wanfang Database,VIP,PubMed and EBSCO,randomized controlled trials (RCT) about lidocaine combined with ropivacaine versus lidocaine alone or ropivacaine alone for anesthesia were collected. Meta-analysis was performed by using Rev Man 5.0 software provided by the Cochrane Collaboration after data extracting and quality evaluating. RESULTS:Totally 30 RCT were included,involving 2 442 patients. Results of Meta-analysis showed,lidocaine+ropivacaine vs. lidocaine:onset time was 6.77 min vs. 7.11 min [MD=-0.29,95%CI(-0.43,-0.16),P<0.001],maintenance time was 5.11 h vs. 2.11 h [MD=3.13,95%CI (3.02,3.25),P<0.001] and the incidence of adverse reactions was 28.6% vs. 44.6% [MD=0.43,95%CI(0.31,0.62),P<0.001];lidocaine+ropivacaine vs. ropivacaine:onset time was 9.49 min vs. 15.85 min [MD=-4.43,95%CI(-4.63,-4.22),P<0.001];maintenance time was 4.84 h vs. 6.79 h [MD=-0.61,95%CI(-0.71,-0.51),P<0.001];the incidence of adverse reac-tions was 44.8% vs. 48.1% [MD=0.82,95%CI(0.56,1.19),P=0.30]. Sensitivity analysis showed that the results were in stabili-ty. CONCLUSIONS:Lidocaine combined with ropivacaine shows rapid onset and long duration in anesthesia,and it can reduce ad-verse reactions play their respective advantages.