Objective To analyze risk factors and clinical manifestation of coronary lesions in patients with premature coronary artery disease.Methods 128 cases with premature coronary artery disease were selected as CAD group. 128 healthy persons were enrolled into control group from our hospital. The risk factors and clinical manifestation of coronary lesions in patients with premature coronary artery disease in two groups were compared. Results The risk factors of BMI ,hypertension,smoking,fat and familial history of coronary artery disease in two groups were significantly different ( P ＜ 0. 05 ). The levels of TC,TG, LDL-C, ApoB, Lp, Fib of the CAD group were higher than the control group. These factors of two groups were significantly different(P ＜0. 05). The levels of HDL-C and ApoA1 of the CAD group were lower than the control group. These factors of two groups were significantly different ( P ＜ O. 05 ).Conclusion Patients with premature CHD had many clinical cardiovascular risk factors. Preventing CHD to control the cardiovascular risk factor was important.

Objective To investigate the safety and short-term clinical outcomes of percutaneous targeted positioning bone cement augmentation treatment for osteoporotic compression fracture nonunion. Methods A total of 32 cases of osteoporotic vertebral compression fracture nonunion, with clinical course of more than 6 months between September 2009 and September 2012 were selected. Under local anesthesia and radiological monitoring, targeted positioning puncture was carried out to inject bone cement to strengthen the lesions of nonunion. Visual analogue scale (VAS), activities of daily living (ADL) and radiographic results were compared among preoperative 1 day and postoperative 1 day, 3 months, 6 months, and 12 months. Results The operation was successfully completed in 34 vertebrae of the 32 cases. Postoperative radiographic observation found no cement leakage. As compared to preoperative level, the VAS scores significantly deceased at postoperative 1 day, 3 months, 6 months, and 12 months (P<0. 05), with significant decrease from postoperative 3 months to 12 months (P<0. 05). At postoperative 3 months, 6 months, and 12 months, the ADL scores significantly increased as compared to preoperation (P <0. 05). No significant differences were seen in vertebral anterior and middle height between the preoperation and postoperation (P>0. 05). Conclusion Percutaneous targeted positioning bone cement augmentation treatment for osteoporotic compression fracture nonunion is safe and effective.

Purpose To investigate the clinical efficacy of percutaneous radiofrequency ablation and microwave ablation in the treatment of primary hepatic carcinoma.Materials and Methods Ninety-two patients with primary hepatocellular carcinoma (116 lesions) were randomly divided into 46 cases of radiofrequency ablation group and 46 cases of microwave ablation group, which were treated with radiofrequency ablation and microwave ablation, respectively, the ablation points and ablation time, complete ablation rate, local tumor progression rate, postoperative complications, hospitalization time, hospitalization expenses, recurrence rate and relapse-free survival rate 1 year, 2 years and 3 years after surgery were compared and analyzed.Results There was no significant difference between the two groups in average ablation points (P>0.05), and the ablation time of microwave ablation group was significantly shorter than that of radiofrequency ablation group (P<0.01). There was no significant difference in complete ablation rate and local tumor progression rate between the two groups (P>0.05). The difference of complete ablation rate and local tumor progression rate between the two groups of tumor <3 cm, tumor 3-5 cm and tumor near the vessel were not statistically significant (P>0.05). There were no significant differences in the incidence of fever, pain in liver area and average length of hospitalization between the two groups (P>0.05). The average hospitalization cost of microwave ablation group was significantly lower than that of radiofrequency ablation group (P<0.01). There was no statistically significant difference of the cumulative recurrence rate for 1 year, 2 years, 3 years after surgery between the two groups (P>0.05). There was no significant difference between the two groups in the overall relapse-free survival rate and relapse-free survival rate among tumors with different size of the two groups (P>0.05).Conclusion Using microwave ablation for the treatment of primary hepatocellular carcinoma shows a clinical efficacy and safety as good as radiofrequency ablation. Radiofrequency ablation may have better therapeutic effect for smaller tumors, but microwave ablation may have some advantages for medium or large tumors and tumors located near the vessels.

Objective:To investigate the expressions of tumor stem cell marker CD133 and tumor vascular endothelial cell marker CD105 in lung cancer tissue and their clinical significance.Methods:Streptavidin-biotin-peroxidase complex (SABC)-immunohistochemical staining was used to detect the expression of CD133 and CD105 in 65 cases of lung cancer tissue and 30 specimens of adjacent non-cancerous tissue. The relationship between the expression of CD133/CD105 and tumor size, histological types, differentiation, TNM stage, lymphoid metastasis, and prognosis were analyzed. Results:The positive expression rates of CD133 and CD105 in lung cancer tissues were significantly higher than those in adjacent non cancerous tissues, respectively (69.2% vs 26.7% and 67.7% vs 10.0%, both P<0.05). The positive expression rates of CD133 and CD105 in the group with lymphoid metastasis were higher than those with non lymphoid metastasis, respectively (82.5% vs 48.0% and 80.0% vs 48.0%, both P<0.05). The expressions of CD133 and CD105 were positively correlated with lymphoid metastasis with Pearson coefficient r of 0.35 and 0.32, respectively (P<0.05). No significant correlation was found between the expression of CD133/CD105 and tumor size, histological types, differentiation degree as well as TNM stage(P>0.05). The expression of CD133 was positively correlated with the expression of CD105 in lung cancer tissue with Pearson coefficient r of 0.37 (P<0.05). Postoperative median survival periods of CD133 and CD105-positive group were significantly shorter than the CD133 and CD105-negative groups (37 months vs 66 months,35 months vs 70 months, respectively, both P<0.05). Conclusion:The expression of CD133 and CD105 was associated with lymphoid metastasis and prognosis in patients with lung cancer.Their overexpression implies poor prognosis of lung cancer patients.

Objective To select excellent tomato mutants by space treatment and to cultivate directly new varieties for production application and good seed resource.Methods Seeds of tomato cultivar were carried into space by scientific experiment recoverable satellite.The main agricultural characters of tomato mutant were observed and analyzed.Results Seeds of determinate growth habit tomato cultivar T10-3-2 were carried into space in 2002 by a recoverable satellite(Shenzhou 4).After cultivation and selection,two tomato variants(H1 and H2) and their offspring populations were obtained in SP2.Variations included quantitative character variations such as maturity,fruit characters and fruit trait,as well as genetic character variations such as changes from determinate growth to indeterminate growth of plant growth habits,and from the no shoulders to the green shoulders of fruits.These two kinds of changes were dominant mutations,and could inherit stably.The research also indicated that variations(amplitude,standard deviation and coefficient of variation) of each generation of two mutants,reduced along with generation increasing.It was showed these characters to be easily stable.Conclusion Tomato seeds loaded on spacecraft can truly form the tomato character variation,and the variation character may be stably inherited.

Objective To select excellent tomato mutants by space treatment and to cultivate directively new varieties for production application.Method Growth dynamics and change of agricultural characters of the mutant of indeterminate growth habit was observed.And the enzymogram and activity of two kinds of main isoenzyme were analyzed.Result An indeterminate growth habit mutant(M1) which derived from a determinate growth habit cultivar(10-3-2) was obtained from the 1st generation after space treatment(SP_1).Being an inherited character,the indeterminate growth habit of the mutant can be expressed from generation to generation.The plant height of M1 was 145 cm,42.0 cm higher than that of the background one(increased by 29.6%),when the background plant height was determined.The main stem diameter of the mutant was 1.22 cm,0.34 cm thicker than that of background parent.However,there were no visible difference in the fruit size and fruit set percentage between them.The biological characters of M1 and its progenies were studied,and the results showed that the indeterminate growth have no side effects on the agronomic characters and fruit quality,and the mutant had a potentiality of higher yield if the condition is suitable.The activity of two enzymes,SOD and POD,was assayed and there were no obvious difference of SOD activities and enzymograms between the mutant and its background parent,but POD activity was much lower in mutant than that in its background parent.Conclusion After observation through 3 generations,it is found that indetermination growth habit is inheritable and stable.

Objective To evaluate the indeterminate growth mutant of tomato derived by space mutagenesis to provide the basis for selecting and cultivating the molecular markers of tomato growth habit. Methods Fifty 10-mer randomly amplified polymorphic DNA(RAPD) primers were used to examine the polymorphism of M1 and 10-3-2. Their polymorphic fragments were cloned, and then were transferred to SCAR markers. Results Of all the 50 10-mer RAPD primers, 44 primers amplified polymerase chain reaction(PCR) products and 2 primers (S165 and S168) amplified stable reproducible polymorphic products. The molecular weight of the specific amplified products were 300 bp and 1 500 bp respectively, therefore they were named as TRS165300 and TRS1681500 temporarily. And TRS1681500 was transferred into stable sequence characterized amplified region(SCAR) marker and this marker could be a specific genetic marker of this indeterminate growth habit mutant. Conclusion Space mutation can produce mutants at DNA level from the loaded materials. The indeterminate growth mutant of tomato is derived by space mutagenesis which can provide a valuable material for studying the regulation and control of tomato growth habit.

BACKGROUND:When the intervertebral disc is under stress, the hydraulic pressure generated inside the nucleus pulposus makes the annulus fibrosus extend outward and expand, and the annulus colagen fibers are stretched so that the extracelular matrix of annulus fibrosus cels is also under the pressure. In the intervertebral disc, aggrecan is the main component of proteoglycans, matrix metaloproteinase-2 is a major enzyme for extracelular matrix degradation, and tissue inhibitor of metaloproteinase is a multifunctional specific inhibition factor for matrix metaloproteinase activity. There is a mutual regulation between the latter two to keep the homeostasis between them.
OBJECTIVE: To investigate the mechanism of cyclic tensile strain in the metabolism of intervertebral disc annulus matrix.
METHODS:Rat anulus fibrosus cels were subjected to 2% or 10% cyclic tensile strain at 1.0 Hz for 2 and 12 hours using Flexcel4000 tension system. Then cels were colected and cultured in conditioned medium for gene and protein detection. Real-time quantitative PCR was used to detect mRNA expression of aggrecan, matrix metaloproteinases-2 and tissue inhibitor of metaloproteinase-2. Gelatin zymography was used to detect matrix metaloproteinases-2 activity.
RESULTS AND CONCLUSION:The use of 2% cyclic tensile strain had no obvious effect on the stress fiber of actin cytoskeleton, whereas actin cytoskeleton was depolymerized in response to 10% cyclic tensile strain. The 2% cyclic tensile strain raised the expression of Aggrecan at 12 hours; whereas raised the matrix metaloproteinases-2 and tissue inhibitor of metaloproteinase-2 at 2 hours, both of which were in homeostasis; matrix metaloproteinases-2 activity had no significant changes. 10% cyclic tensile strain had no effect on the mRNA expression of Aggrecan. No matter stretching 2 or 12 hours, the matrix metaloproteinases-2 was up-regulated, and the tissue inhibitor of metaloproteinase-2 was down-regulated, both of which were not in balance. Moreover, the matrix metaloproteinases-2 activity was not significantly changed. These findings indicate that the mRNA expressions of Aggrecan, matrix metaloproteinases-2 and tissue inhibitor of metaloproteinase-2 alter in response to cyclic tensile strain in rat anulus fibrosus cels, and the tensile strain induces different mechano-responses in the actin cytoskeleton.

Objective To explore the effect of recombinant pEGFP-N3-APC vectors carrying various APC functional domains on the expression of β-catenin in human colorectal cancer cells HT-29.Methods The recombinant plasmids were transfected into HT-29 cells mediated by lipofectamine~(TM) 2000, and detected by green fluorescence and RT-PCR. Western blot was applied to detect β-catenin expression level in HT-29 cells after transfection, and gray scales of electrophoresis strips were analyzed by SPSS 13.0.Results Green fluorescence and RT-PCR made clear that all 5 recombinant plasmids were successfully expressed in HT-29 cells. Western blot showed that β-catenin expression level in HT-29 cells was not affected after being transfected with pEGFP-N3-APC1, pEGFP-N3-APC2 and pEGFP-N3-APC3, and was distinctly affected after being transfected with pEGFP-N3-APC4 and pEGFP-N3-APC5, especially the later one. Conclusion The selected APC5 gene fragment with 15-amino acid repeats and SAMP repeats, which is relatively short, can degrade β-catenin level in HT-29 cells and may be applied in the gene therapy.

BACKGROUND: Osteoporosis is easily seen in postmenopausal women. The bone loss situation is different among populations of different age and body mass.OBJECTIVE: To analyze the effects of age, menopausal duration,menopause age, height, body mass and body mass index on bone mineral density (BMD) in postmenopausal women.DESIGN: Random sampling test was conducted to postmenopausal women.SETTING: A clinical trial center of a provincial Chinese traditional medicine research institute, an orthopaedic department of a university hospital and a bone wound department of a university.PARTICIPANTS: Totally 603 naturally postmenopausal women in Fuzhou area from September 2000 to August 2003.METHODS: Random sampling method was used. The age of participants,menopausal duration, menopause age, height, body mass and bone mineral index(BMI) were recorded. Dual energy X-ray bone mineral density machine was used to examine the bone mineral density of lumbar, neck of femur, great trochanter and Ward' s area while SPSS software was used to conduct regression analysis.MAIN OUTCOME MEASURES: The correlation between age,menopausal years, menopause age, height, body mass, BMI and bone density as well as the regression equation.RESULTS: There was very significant correlation between age, menopausal duration, body mass, BMI of postmenopausal women and bone mineral density of lumbar, neck of femur, great trochanter and Ward' s area. The BMD in low body mass group is greatly lower than that of obesity group( P ＜ 0.01 ). The main factors impacting BMD of lumbar are in turn age, body mass and menopause age while the regression equation: y = 0. 927 - 0. 0093 X1+ 0. 0037X2 + 0. 004X3. The factors impacting BMD of neck of femur are in turn menopausal duration, body mass, menopause age and the regression equation was: y = 0. 687 - 0. 0081 X1 + 0. 0048 X2 - 0. 0034 X3. And the factors affecting BMD of greattrochanter are age, body mass and menopause age while the regression equation was y = 0. 591 - 0. 0038 X1 + 0. 0042 X2 - 0. 0024 X3. Age, body mass index and menopausal duration are main factors affecting BMD of Ward' s area and the regression equation was y =0. 686 -0. 0072 X1 +0. 0136 X2 -0. 0046 X3.CONCLUSION: The BMD of lumbar and hip gradually decreases with the increase of age in postmenopausal women. The risk of getting osteoporosis in people with low body mass is greater than women in normal body mass groupand obesity group.