Objective: To investigate the possible effects of Pancreatic duodenal homeobox-1((Pdx-1))expression in transdifferentiation of bone marrow mesenchymal stem cells(MSC) in vitro. Methods: The eukaryotic expression vector containing Pdx-1 was constructed.After such vector was transfected into MSC using Superfect,G418 was used to select the positive cells.Then both(Pdx-1~(+)MSC)and((Pdx-1~-MSC)) were induced to transdifferentiate in vitro.The expressions of insulin were analyzed by immunohistochemistry staining. Results: Restricted enzyme analysis and sequencing showed that interest gene segment was consistent with that in Gen Bank,Recombination vector was effectively transformed into MSC demonstrated by fluorescence microscope;insulin-producing cells from Pdx-1~(+)MSC were higher than that from Pdx-1~(-)MSC[(28.23?2.56)% and(7.08?2.69)%,respectively]. Conclusion:Pdx-1 can promote adult rat MSC to transdifferentiate into insulin-producing cells in vitro,and this approach might lead to a widespread cell replacement therapy for type I diabetes.

Objective To investigate the effects of dexmedetomidine on apoptosis and CHOP in lung injury following one lung ventilation in SD rats.Methods 60 SD rats were randomly divided into the control group,OLV combined with 0.9%sodium chloride injection,and OLV combined with dexmedetomidine group with 20 rats in each group.The rats were killed after the detection of apoptosis index,and the proportion of wet and dry GRP78 and CHOP protein and gene expression level.Results Fluorescence microscope observed cell shrinkage,increased cytoplasmic density,mitochondrial membrane potential disappearance, permeability changes.Vesicular membrane had formed, membrane integrity,and could be wrapped apoptosis remains divided into several apoptotic bodies.3 groups apoptotic index,the proportion of wet and dry lung tissue had statistically significant differences ( F=9.675,4.357,all P<0.05).The apoptosis index of OLV combined 0.9%sodium chloride injection group and OLV combined with dexme-detomidine group,the proportion was higher wet lung tissue,OLV combined 0.9% sodium chloride injection group was higher than the OLV combined with dexmedetomidine group, the differences were statistically significant ( t =4.432,3.458,all P<0.05).3 group GRP78 protein,GRP78mRNA,CHOP protein,CHOPmRNA levels had statisti-cally significant differences(F=4.350,5.897,5.875,6.785,all P<0.05),OLV combined 0.9% sodium chloride injection group and OLV combined with dexmedetomidine group GRP78 protein, GRP78mRNA, CHOP protein, CHOPmRNA were higher than those in the control group,OLV combined 0.9%sodium chloride injection group was higher than the OLV combined with dexmedetomidine group,the difference was statistically significant(P<0.05). Conclusion Dexmedetomidine given OLV can induce apoptosis and reduce the expression levels of CHOP,indicating damage to the lungs caused by a protective effect OLV.

In this study, it was found that in uncontrolled diabetes mellitus serum T3 and FT3J were decreased, and rT3. elevated. Fasting blood glucose and HbA1c were in negative correlation with T3 and FT3I. and in positive correlation with rT3. Serum TSH was negatively correlated with degree of retinopathy, 24h urinary protein, and duration of diabetes. TRH stimulation test showed low, delayed, or no response in 57% of patients tested, and the resultswere in negative correlation with fas'ting blood glucose and HbA1c. After 1-4 month trentment,blood glucose decreased, serum T3 increased, and rT3 decreased. It was believed that thyroid function test is of significance in evaluating the severity, prognosis, and effect of treatment in diabetes mellitus

Objective:To evaluate the effect of ketamine on the expression level of hepatic stress protein IFIT1 and JAB1 during the early stage of burns in mice, and observe the location of IFIT1 and JAB1 in hepatic cells. Methods:15 C57/129 male mice were divided randomly into three groups(n=5): normal control,burns,burns+ketamine. Burns group and burns+ketamine group were inflicted with 15%~20% TBSA full thickness burn injury,and burns+ketamine group received an intramuscular injection of 10 mg/kg ketamine 15 min after burns. At 4 h after burns,hepatic tissue was taken from mice,and the levels of hepatic I- FIT1 and JAB1 were detected by western blot. Normal control hepatic pathological section was taken; then cell location of I- FIT1 and JAB1 was detected by immunohistochemistry. Results:In burns group, the expression level of hepatic IFIT1 signifi- cantly increased,while that of JAB1 decreased as compared with normal control(P

Objective To evaluate the development of the sphenooccipital synchondrosis on high resolution CT scans.Methods 269 cases,HRCT findings of the sphenoocipital synchondrosis ranged in age from 3 months to 74 years old were analysed.Four points were assessed on HRCT scans (a) The pattern of the synchondrosis, (b) The location and time of appearance of the ossification center in the synchondrosis, (c) The vestige of the synchondrosis after fusion of the sphenoid and occipital bones and (d) The development of sphenoid sinus.Results The pattern of the sphenooccipital synchondrosis could be classified into three types, including complete opening, partial and complete closure. The spheno-occipital synchondrosis was variable in shapes in opening group,a notch existed in 57 cases, the mean of the thickness of the spheno-occipital synchondrosis was 1.8 mm, there was negative correlation between the thickness of the synchondrosis and the age (r= -0.92,P

Theory of the spleen and stomach is an important component of TCM.In the article,the author introduced the principal knowledge of spleen and stomach in TCM,the recognition of AIDS in TCM,the application of theory of spleen and stomach in prevention and treatment for AIDS.It had important significance for theory of spleen and stomach in treating and preventing AIDS.

Objective To study the etiology and clinics as well as CT diagnostic value of periventricular leukomalacia(PVL)in children.Methods Clinical and CT findings in 130 cases of PVL were retrospectively analyzed.Results 85 cases were premature newborn babies(65.4%) and 45 cases were fullmature newborn(34.6%) with hypoxic ischemic encephalopathy.CT showed the lateralventricle enlarge in different degrees and the white matter of periventricle decreased.Conclusion CT scan is of important value indiagnosis of PVL.

Objective To investigate researches on magnet hospital and nursing management from January 1,2010 to December 31,2014 in PubMed database and provide reference information for the majority of professionals in deep research.Methods Bibliographies from research literature of magnet hospital and nursing management from January 1,2010 to December 31,2014 in PubMed database were downloaded.The publication years,journals,countries of publication,the high yielding first authors and the high frequency of main subject were counted by Bicomb 2.0 software.The affiliations were analyzed artificially.main subject appeared no less than two times were intercepted as high frequency ones and the high frequency main subject co-occurrence matrix were formed.SPSS20.0 statistical software were applied for clustering analysis with matrix,then to get the topic hotspots.Results A total of 62 literatures were screened out.The data of research trend,journals,research degree of countries were acquired.The number of high frequency main subject were 18 and among which 4 research hotspots were clustered,respectively workplace environment and job satisfaction,nursing education,nursing quality administration,creation and leadership.Conclusions Researches on magnet hospital and nursing management from January 1,2010 to December 31,2014 in PubMed database are mainly in terms of workplace environment and job satisfaction,nursing education,nursing quality administration,creation and leadership.They are the foundation of the current research situation and trend of magnet hospital nursing management,and can improve management efficiency and provide reference for scientific research topic selection project.

Obejective To analyze the related papers on nursing competency in China from 2004 to2014,and to learn the current status and developing trends of nursing competency,so as to provide reference for further research and development of nursing competency.Methods All literature related to nursing competency from 2004 to 2014 were searched in the China National Knowledge Infrastructure(CNKI),VIP Database for Chinese Technical Periodicals (VIP),and Wanfang database.Bibliometric analysis methods were used to analyze the distribution time,area,journal distribution and research categories by using Microsoft Excel.Results Totally 199 articles related to nursing competency were collected and the number of publications increased in recent years.About 78.89％ (157/199) papers were published in the statistical source journal and 34.17％ (68/199) papers were focused on survey research about nursing competency.Conclusions Nursing competency have been paid more attention to,but the quality of the literature still need to be improved.

Objective To investigate the charge nurses' leadership style perceived by nurses and nurse's self-efficacy,and then analyze the relationship between them.Methods Totally 368 staff nurses from 3A hospitals in Sichuan were investigated with questionnaires.The results underwent analysis.Results The score of charge nurses' transformational leadership style was 58.06±12.10,from high to low in turn was intellectual stimulation (3.10±0.69),inspirational motivation (2.92±0.73),idealized influence (2.88±0.67) and individualized consideration (2.71±0.74).The score of charge nurses' transactional leadership style was 35.77±8.58,from high to low in turn was contingency reward (3.11±0.71),passive exemplary management (3.09±0.75) and active exemplary management (2.75±0.90).The score of nurses' self-efficacy was 83.51±11.62.The transformational leadership style had positively predictive impact on nurses' selfefficacy.Conclusions Nursing managers should apply transformational leadership to improve nurses selfefficacy,promote the physical and mental health of nurses and the long-term development of hospital.