Objective:To investigate the effects of granulocyte-macrophage colony-stimulating factor on expressions of CC chemokine receptor 2(CCR2) mRNA and protein in cultured human THP-1 cell lines.Methods:THP-1 cell was stimulated by GM-CSF of different concentrations and time intervals.The mRNA expression of CCR2 was tested by RT-PCR,The protein expression of CCR2 was analyzed by Western blotting.Results:RT-PCR and Western blotting showed that THP-1 cell in control group expressed a low-level of CCR2 mRNA and protein.After stimulated by GM-CSF,the expression of CCR2 mRNA and protein increased in a concentration-and time-dependent manner.Conclusion:GM-CSF could stimulate THP-1 cell to express CCR2 mRNA and protein,in an concentration-and time-dependent manner.

In recent years,the prognosis and survival quality of tumour patient would be improved greatly through cell immunotherapy,which becomes the priority scientific research.Immunoeytes can kill the tumour cell directly or through immunoregulation.CD4CD8 double-negative T cells is a newly found subset of T cells whose SUlface express neither CD4 nor CD8.This article mainly introduces the advancement of one subet of DNT cell,DN Tregs,in tumor.

Objective To investigate the effects of lipid microspheres prostaglandin E1(Lipo PGE1) on subarachnoid hemorrhage(SAH).Methods Ninty-three patients with SAH were randomly divided into control group(treated with nomal drugs) and Lipo PGE1 treatment group(treated with nomal drugs and Lipo PGE1).Changes in neuroimaging,biochemical indexes and incidence of cerebral vasospasm were measured. Results A lower incidence rate of cerebral vasospasm was observed in Lipo PGE1 treatment group(P0.05).The levels of vWF and GMP-140 were significant lower in the Lipo PGE1 treatment group than those in the control group after treatment for 3 and 7 d(P

Objective To investigate the changes of somatosensory evoked potential(SEP)in subarachnoid hemorrhage(SAH)and in-fluence from lipid microspheres prostaglandin E1(Lipo PGE1). Methods Ninty-three patients with SAH were randomly divided into control group(treated with nomal drugs) and Lipo PGE1 treatment group(treated with nomal drugs and Lipo PGE1).Clinical outcomes and changes of SEP before and after the treatment were observed. Results After the treatment,the latency of N20 wave was prolonged in both groups(P

This study examined the difference in dietary intake between middle−aged and elderly Japanese and Chinese. Volunteers aged 50∼79 living in two cities in both Japan and China were recruited in local community service centers and were asked to complete a 3−day diet recording. The final results were based on 356 subjects (166 Chinese and 190 Japanese). In men, the Japanese subjects significantly consumed more energy, with a large proportion of the energy coming from carbohydrates, dietary fiber, vitamin A, vitamin B2, vitamin C and they consumed less iron, and a lower proportion of energy from protein and fat than in the Chinese subjects. In women, the Japanese subjects had significantly higher intakes of energy, the proportion of energy from carbohydrates, dietary fiber, calcium, vitamin A, vitamin B1, vitamin B2, vitamin C and they consumed less iron, with the proportion of energy from protein and from fat in comparison with the Chinese women. Fat intake provided more than 29% of the total dietary energy in Chinese subjects. Daily intake of food items was significantly greater in the Japanese participants, both men and women (18.54±2.85, 20.11±2.40, respectively), than in the Chinese subjects (14.11±3.62, 15.51±4.15, respectively) (p<0.01 by Mann Whitney U−test). The present study suggests that the middle−aged and elderly Chinese subjects should increase their variety of foods consumed while decreasing their consumption of high−fat foods. For the Japanese subjects, the higher intake of total calories among the woman should also be noted.
Chinese People ; Japanese language ; Energy, Physics ; Human Females ; Elderly

AIM To study protective effect of [D-Ala~2, D-Leu~5]-enkephalin (DADLE) against hydrogen peroxide (H_2O_2) induced myocardial cell injury and its possible mechanisms. METHODS Myocardial cells were isolated from neonatal rats and cultured for 48 h. Then the cells were randomly assigned into normal control, H_2O_2（200 μmol·L~(-1)）, H_2O_2+DADLE(1 μmol·L~(-1)), H_2O_2+DADLE +naltrindole(10 μmol·L~(-1)) and H_2O_2+DADLE +U0126(10 nmol·L~(-1)) groups and cultured for another 48 h.[~3H]TdR incorporation assay and flow cytometry were used to measure the cell proliferation and apoptosis rate. The lactate dehydrogenase (LDH) activities in culture supernatant measured by using LDH activity kit. The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in cells were measured with xanthine oxidase method and color reaction of thiobarbituric acid, respectively. The expressions of extracellular signal-regulated kinase (ERK) and phosphorylated-ERK (p-ERK) were observed with Western blot. RESULTS ① Compared with normal control group, the incorporation of [~3H]TdR in myocardial cells of H_2O_2 group was significantly lower, apoptosis rate was higher, LDH activity and MDA content in cells were higher, while SOD activity in cells was lower. In addition, the ratio of IA_( p-ERK) /IA_( ERK) was decreased. ② Compared with H_2O_2 group, the incorporation of ［3H］TdR in H_2O_2+DADLE group was significantly higher, apoptosis rate was lower, LDH activity and MDA content in cells decreased, while SOD activity increased significantly. The ratio of IA_( p-ERK) /IA_( ERK) was increased. ③ δ-Opioid receptor antagonist naltrindole and ERK antagonist U0126 inhibited this effect of DADLE on the above index changes induced by H_2O_2. CONCLUSION The δ-opioid receptor has protective effect against H_2O_2-induced myocardial cell injury, and its possible mechanism may be related to its promotion of antioxide capacity and ERK phosphorylation.

Objective To investigate the effects of different blood pH of the recipients on ischemiareperfusion (I/R) injury to the transplanted lungs in rats. Methods Sixty-four pure bred male SD rats weighing 250-350 g were randomly divided into equal numbers of donors and recipients and the recipient rats were randomly divided into 4 groups(n = 8 each). Both donor and recipient rats were anesthetized with intraperitoneal (IP)ketamine 15 mg/kg and diazepam 6 mg/kg, tracheostomized and mechanically ventilated. In donor rats heparin 1 ml was injected via inferior vena cava and 4 ℃ LDP solution 40 ml was injected via pulmonary artery . The left lung was then removed and placed in 4℃ LDP solution and kept for 4 h. The left lung transplantation was performed using two-cuff-one-stent anastomosis technique. Before the transplanted lung was reperfused, the recipients' blood pH was adjusted to 7.4 (group Ⅰ), 7.2 (group Ⅱ), 7.3 (group Ⅲ) and 7.5 (group Ⅳ) by HCI and NaHCO3 solutions infused via femoral vein. After 2 h reperfusion, the transplants were removed for microscopic examination and determination of W/D lung weight ratio, MPO and SOD activities and MDA, TNF-α and IL-8 contents. Results SOD activity was significantly higher while MPO activity, and MDA, TNF-α and IL-8 contents in the lung tissue were significantly lower in group Ⅲ than in group Ⅰ . There was no significant difference in SOD and MPO activities and MDA, TNF-α, and IL-8 contents in the lung tissue among group Ⅰ , Ⅱ and Ⅳ. There was no significant difference in W/D lung weight ratio among the 4 groups. Microscopic examination showed that the histological damage was attenuated in group Ⅲ compared with the other 3 groups. Conclusion The I/R injury to the transplanted rat lungs can be effectively alleviated by reducing blood pH of the recipients to 7.3.

Objective To prepare a targeted ultrasound micro bubble,which carried the HSV-TK gene,and investigate the in vitro target searching ability of the micro bubbles and inhibitory effect on the proliferation of HepG2 cells.Methods Ultrasonic micro bubbles were prepared by mechanical vibration method,construction of targeted HSV-TK ultrasound micro bubbles by biotin affinity bridge construction.To detect the general characteristics of ultrasound micro bubbles,and to test its effect on the proliferation of HepG2 cells in vitro.Results HSV-TK targeted ultrasound microbubbles more gathered on the surface of HepG2 cells,through detection of PCNA and MTT,it was found that the proliferation of gene targeting microbubble group was obviously decreased,cell apoptosis increased significantly,Cells invade experiments showed that the number of cells in genetic microbubble group (22.18 ± 2.01) decreased significantly compared with the control group and the nontargeted group,can effectively inhibit the proliferation and invasive ability of HepG2 cells.Conclusion Targeted ultrasound microbubble carrying target gene have better inhibitory effect on HepG2 cells in vitro.

0.05). The products of conjunction of cagⅠ and cagⅡ were found only in 5 strains. The detectable rate of continuous cag PAI was much higher in duodenal ulcer than in chronic gastritis ( P

Objective To explore the clinical significance of detecting serum sCD44V6 in non?- small cell lung carcinoma. Methods Levels of serum sCD44V6 in 42 patients with lung carcinoma and in 30 healthy persons were measured by ELISA. Results There was obvious difference of serum sCD44V6 levels between patients with lung carcinoma and healthy persons. There was no difference between patients with squamous cell carcinoma and with adenocarcinoma. sCD44V6 have the higher positive rate and specificity. Conclusions sCD44V6 would be a good way to non?- small cell lung carcinoma, and could be useful to the diagnosis of early?- stage non?- small cell lung carcinoma.