We only prolongated the course of shock from 2h to 5h, without predi- sposing the dogs. At 5h after hemorrhagic shock, the experimental results were asfollows: The ultrastructural damages were not only detected in the lung but also in theheart, liver, intestine and kidney etc. Major pathological lesions were: swelling of paren-chymal cells in various organs, vacular degeneration, and de-connection of cells. The laterwas shown as disintegration of hepatocyte-conjunction and disappearance of microvilli. Theorganelles of various cells ruptured to different degrees, for example, mitochondria swelledand its crista dissolved, endoplasmic reticulum dilated and de-granulated. In addition, thefibril of cardiac muscle thinned and dissolved, the capillary endothelial tight-conjunctionsof lung and intestine ruptured etc. The quantity of entero-endotoxin in plasma increasedin accordance with prolongation of shock, and its quantity was most abundant in portalvein, the next was artery and the last was hepatic vein. All experimental results showedthat the entero-endotoxin was related to the changes of ultrastructure of various organs.

Compared to normal counterparts,cancer cells exhibit metabolic changes owing to both genetic and epigenetic alterations. Some abnormal alterations happen in tumor cells including glycolysis,mito-chondrial biogenesis,glutaminolysis and lipid synthesis. These metabolic changes have great significance to development of tumor and clinical targeted therapies.

Objective To investigate effect of edaravone on serum inflammatory factors, 6-keto-prostaglandin F1α(6-keto-PGF1α), thromboxane B2, endothelial function and serum copeptin and N-terminal brain natriuretic peptide (NT-proBNP) in patients with acute cerebral infarction.Methods From March 2013 to September 2014, 213 cases of acute cerebral infarction were selected in the hospital and randomly divided into control group (n=101) and observation group (n =112).Control group were given conventional symptomatic treatment, and observation group were given edaravone injection on the basis of control group.The serum inflammatory cytokines, 6-keto-PGF1α, thromboxane B2, endothelial function, serum copeptin, NT-proBNP and nerve function score and activities of daily living ( ADL) score were compared between two groups.Results Serum CRP, IL-8, IL-10 in observation group after treatment were significantly lower than control group (P<0.05).Plasma thromboxane B2 in observation group after treatment was significantly lower than control group (P<0.05).The levels of 6-keto-PGF1αwas significantly higher than control group (P<0.05).Serum copeptin and NT-proBNP levels in observation group after treatment were significantly lower than control group (P<0.05).Plasma ET-1 in observation group after treatment was significantly lower than control group ( P<0.05 ) , and plasma NO was significantly higher than the control ( P<0.05 ).Neurological function in observation group after treatment was significantly lower than control group (P<0.05), and ADL score was significantly higher (P<0.05). Conclusion The preliminary study shows that edaravone in treatment of acute cerebral infarction may be associated with decreasing serum inflammatory cytokines, increasing 6-keto-PGF1αand reducing thromboxane B2, improving endothelial function and reducing serum copeptin and N-terminal natriuretic peptide.

AIM: To investigate the effects of low-dose theophylline on differentiation of the naive T lymphocyte. METHODS: Naive T lymphocytes and monocytes were isolated from cord blood in neonate. Theophylline (10 mg?L-1 ) was added in naive T lymphocytes and monocytes which stimulated by PHA (200 mg?L-1 ) for 3 days, and the differentiation of T cells was analyzed by flow cytometry. Cytokines were measured by enzyme linked immune-sorbent assay ( ELISA ). RESULTS: Theophylline significantly promoted differentiation fromTh1 cells to naive T cells ( P

Objective To investigate impact of rosuvastatin on cardiac function, lipid levels, blood rheology level and endothelial function in patients with coronary heart disease.Methods 149 cases were randomly divided into two groups, the treatment group (n=76) and control group(n=73).Control group with conventional symptomatic treatment, treatment group on the basis of control group combined rosuvastatin treatment.Cardiac function, blood lipid levels, blood rheology changes and endothelial function levels before and after treatment were comparative analysis.Results LVEF significantly increased after treatment than before treatment between two groups (P<0.05), while LVESV, LVEDV after treatment than before treatment were significantly lower ( P<0.05 ) , LVEF in treatment group was significantly higher than control group ( P<0.05 ) , LVESV, LVEDV was significantly lower than that of control group (P<0.05).The two groups of LDL-C, TC, TG after treatment was significantly lower than before treatment (P<0.05), but HDL-C was higher than before treatment(P<0.05).LDL-C, TC, TG in treatment group after treatment was significantly lower than control group (P<0.05), whereas HDL-C after treatment was significantly higher than control group (P<0.05).Fibrinogen, plasma viscosity, whole blood low shear viscosity, whole blood viscosity was lower than that before treatment (P<0.05), which in treatment group was significantly lower than control group (P<0.05).NO, FMD was significantly higher than before treatment (P<0.05), and ET was significantly lower than before treatment (P<0.05).NO, FMD in treatment group was significantly higher than control group (P<0.05), and ET was significantly lower than control group (P<0.05).Conclusion Rosuvastatin can significantly improve cardiac function, improve blood lipid levels, lower levels of blood rheology, improve endothelial function.

Her-2 receptor has already been identified as one of the most important biological markers of some malignant tumors,and also plays an important role in the biological behavior of those tumor cells.The over-expression of Her-2 receptor is associated with radioresistance of various tumor cells,and thus an antibody to Her-2/neu receptor can probably function as a radiosensitizer.In this review,we summarized some advances in the molecular mechanism and clinical aspects of the relationship between Her-2 and cellular radiosensitivity.

OBJECTIVETo investigate the changes of cardiac T troponin (cTnT) and high-sensitivity C reactive protein (hs-CRP) in children with viral myocarditis (VMC).

METHODSForty children with VMC were examined for cTnT, hs-CRP, and creatine kinase MB (CK-MB) levels.

RESULTSChildren with VMC had significantly higher cTnT, hs-CRP and CK-MB levels than the control group on admission (P<0.01), but obviously decreased after two weeks of treatment. The positivity rate of cTnT and hs-CRP were significantly higher in children with VMC than the control group on admission, and decreased significantly after treatment (P<0.01). The positivity rate of cTnT and hs-CRP were significantly higher than that of CK-MB (P<0.05).

CONCLUSIONSerum cTnT and hs-CRP are sensitive indices for diagnosis of VMC, and their detection have important value in estimation of the patients' condition.

Biomarkers ; blood ; C-Reactive Protein ; metabolism ; Child ; Child, Preschool ; Creatine Kinase, MB Form ; blood ; Female ; Humans ; Male ; Myocarditis ; blood ; virology ; Troponin T ; blood ; Virus Diseases ; blood

AIM and METHOD: In terms of difference value between bleeding blood volume that caused hemorrhagic shouk (HS) and residual blood volume at 2 h after HS, showed that HS at 5.3 kPa level was compen- satory and at 4.0 kPa level was decompensatory. Comparing some blood changes between HS two levels and their changes while pretreated with captopril (Capt. ) to reduce the release of angiotensin Ⅱ (Ang-Ⅱ), so as to inveshgate the significance of Ang - Ⅱ during HS. RESULTS: The residual blood volume in 4.0 kPa HS + Capt. group are again from near "zero" value in simple 4.0 kPa HS group. In both two HS level groups found blood dilution and was not influenced by pretreating with Capt.; contents of K+ and aldosterone increased, but Na+ had no changes, in Capt. + HS group, the former two contents reduced and Na+ had no changes comparing with each HS group. In two HS groups, the bind lactate, lactic dehydrogenase (LDH), blood urea nitrogen (BUN) increased and had more increment in 4.0 kPa HS group. All these changes could be prevented by pretreating with Capt. The blood glucose in 5.3 kPa HS group increased markedly and Capt. had no influence on it, but decreased extremely in 4.0 kPa HS group and Capt. could make it re - increased. CONCLUSIONS: Artery blood pressure (ABP) at 5 .3 kPa level was compensatory HS and ABP at 4 .0 kPa level was decompensatory HS, some changes on decompensatory HS were more serious and severe than compensatory HS, Capt. has protective effects on some changes during HS and could prolong the survival time of decompensatory HS, all that indicated the increment of Aug - Ⅱ is an important pathogenetic factor during HS.

Objective Castleman′s disease ( CD) is a rare lymphoproliferative disorder, which has a very high misdiagnosis rate according to its lack of clinical and imaging specificity.The study was to improve the recognition, diagnosis and management of this disease by analyzing the clinicopathological characteristics and concluding diagnosis and treatment as well as literature review. Methods Retrospective analysis was conducted on clinical data of 31 CD patients (13 males and 18 females) who were hospitalized in the Affiliated Drum Tower Hosipital to Medical college of Nanjing University from January 2006 to Feburary 2014.Their mean age was (47.4 ±13.3) years.There were 24 cases with unicentric Castleman′s disease (UCD) and 7 cases with multicentric Castleman′s disease ( MCD) .UCD tended to be present in the form of an enlarged and painless mass which generally remained asymptomatic by accidental touch or regular physical examination.MCD was usually associated with constitutional symptoms.All the 24 patients with UCD underwent complete surgical resection.7 patients with MCD received different treatment methods, such as surgical resection, ste-riod and rituximab in combination with chemotherapy ( CHOP) . Results Postoperative pathology confirmed 23 cases were hyaline vascular type while 8 were plasma cell type.25 cases had been followed up (46.3 ±32.3) months after operation, among which 1 UCD case died of pancreatic head carcinoma 13 months after operation, 1 case with MCD plus pemphigus died 2 months after surgical resection of retroperitoneal lymphadenectasis, another MCD case died in 1 month without any treatment.The remaining 22 cases were alive. Conclusion CD is lack of clinical specificity and its diagnosis should be made by pathology.Each subtype differ greatly in prognosis, therefore different treatments should be taken after definitive subtype diagnosis.

Urinary albumin used as an important indicator in diagnosis of renal disease , but there are still many problems in the clinical application .The urine specimen type , threshold value , reference measurement procedures and some more aspect were summarized in this article .