Objective To evaluate clinical efficacy of single center intracavitary isolation procedure for treating elderly Standford type B aortic dissection.Methods 46 consecutive patients aged ≥ 65 years with Standford B aortic dissection,who underwent membrane stent intracavitary isolation treatment,in Tianjin Chest Hospital between 2010-2015 were enrolled in this study.All enrolled patients received examinations of echocardiography,contrast-enhanced CT,hepatic and renal functions,and the blood and urine routine tests before the procedure.After the procedure,the contrastenhanced CT of whole aorta was annually rechecked for all patients in the four time points of predischarge,3,6,12 months after operation.Deadline date of the follow up was December 2015.The outcomes of whole aorta CT and survival rate were analyzed.Results Successful stent implantation was performed in all patients (100 ％).Two (4.3 ％) patients died in the perioperative period,and one died due to the new aortic dissection at the last 8 months after operation.Overall survival rate was 89.1％(41 cases)during the follow-up period(95％CI:52.5-63.6 and 32.6-38.9,all P＜0.05).Conclusions The short and long term outcomes of intracavitary isolation procedure for treating Standford B aortic dissection are satisfactory in elderly patients.Perioperative blood pressure control,strict image measure,and carefully selecting the appropriate scaffold model are the keys for successful operation.

ObjectiveTo evaluate the effects of two kinds of hemocoagulases, Reptilase and Baquting, on coagulation function during neurosurgical operation.Methods60 patients scheduled for craniotomy were randomly allocated into 4 groups to receive either normal saline as control (Group Ⅰ, n=15), Baquting 2 kU (Group Ⅱ, n=15), Baquting 4 kU (Group Ⅲ, n=15) or Reptilase 2 kU (Group Ⅳ, n=15) after propofol and isoflurane general anesthesia and before hypervolumic hemodilution with 6% HAES-steril 1000 ml. The blood samples were drown for testing routine blood parameters (Hb, HCT, PLT, RBC, etc.), coagulation parameters (PT, TT, APTT, INR, FIB), and sonoclotactivated clotting time (SonACT), rate of clot formation (CR) and platelet function (PF) using Sonoclot coagulation & platelet function analyzer before anesthesia, after anesthesia, at 20 min (HAES 500 ml infused), 40 min (HAES 1000 ml infused) and 120 min after the test drug was given, at the end of operation, and 24 h after operation.ResultsThere were an obvious prolongation or decrement in almost all parameters in all patients and restored to normal in the first day after operation. But the trends showed less changes in PT, TT, APTT, FIB, PF, CR in every Baquting and Reptilase groups compared with Group Ⅰ.ConclusionMedium hemodilution, coagulation and platelet dysfunctions happened during neurosurgical operation. Hemocoagulase can only partly but not significantly reverse the changes of hemostatic abilities in every group.

OBJECTIVE To validate the anticancer effect of Angong Niuhuang pill (AGNH) and pinpoint associated molecular mechanisms using human cancer cells.METHODS Human MGC-803 gastric carcinoma and human BEL-7402 hepatocarcinoma cells were incubated with AGNH 9,30,90,300 and 900 mg·L-1 for 24,48and 72 h,respectively.Cell viability was detected with 3-(4,5-dimethylthiazol-2-yl) -5-( 3-carboxymethoxyphe-nyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and colony formation assay.Apoptosis was measured with flow cytometry and Hoechst 33258/PI staining.Change in mitochondrial membrane potential (△qψ) was detected by spectrofluorophotometer.RESULTS AGNH inhibited MGC-803 cell proliferation ( for 48 h,r =0.996,P =0.002; for72 h,r=0.756,P=0.024 ) and BEL-7402 cells (for 48 h,r =0.732,P=0.030; for72 h,r=0.702,P =0.037) in a concentration-dependent manner,as showed by MTS assay.AGNH inhibited colony formation on MGC-803 cells (r =0.914,P =0.011 ) and BEL-7402 cells (r =0.871,P =0.024) in a concentration-dependent manner for 24 h.Hoechst 33258/PI staining and flow cytometry assay showed that AGNH 900 mg·L-1 for 24 h induced apoptosis of MGC-803 and BEL-7402 cells,and the apoptosis rate was 27.2％ and 19.7％,respectively.Compared with normal control group,AGNH 900 mg·L-1 for 3 min decreased the mitochondrial membrane potential of MGC-803 and BEL-7402 cells to 15.9％ and 15.0％ of control group.CONCLUSION AGNH inhibits proliferation of human cancer cells.Apoptosis and depolarization of mitochondrial transmembrane potential are probablly its mechanism.

AIM: To observe the effect of simvastatin on the proliferation of vascular smooth muscle cells(VSMCs) induced by serum and growth factor PDGF-BB and the effect of simvastatin on the expression of PTEN,a important regulator of G 1/S cell cycle transition. METHODS: The DNA synthesis was determined by -TdR incorporation, cell cycle was examined with flow cytometry, the protein level of PTEN was measured by Western blot method. RESULTS: (1)Simvastatin inhibited -TdR incorporation in a dose dependent manner. (2) Flow cytometric DNA analysis revealed that simvastatin induced significantly enhancement of G 0/G 1 phase and decrease in S phase VSMCs.(3)Simvastatin increased protein level of PTEN and mevalonate, a metabolite of HMG-COA, reversed the effect of simvastatin on PTEN protein expression. CONCLUSION: Simvastatin may inhibit proliferation of VSMCs and retarded cell cycle in G 0/G 1 phase by increasing PTEN expression through inhibiting synthesis of mevalonate.

AIM: Hydroxymethylglutaryl CoA (HMG-CoA) reductase inhibitors, such as simvastatin, have been shown to reduce atherosclerotic cardiovascular morbidity and mortality by mechanisms unrelated to its lipid-lowering effect. Several studies have shown that simvastatin induces apoptosis in a varieties of cell lines including vascular smooth muscle cells (VSMC). The aim of this study was to investigate the signal pathways involved in apoptosis induced by simvastatin. METHODS: Cultured VSMC were treated with simvastatin. Calpain activity was determined by measuring Ca 2+ ionophore-specific calpain substrate (suc-LLVY-AMC), caspase-3 activation was detected by Western blot, and apoptotic changes were distinguished by annexin Ⅴ binding and DNA laddering. RESULTS: After incubated with 30 ?mol/L simvastatin for 8 h, calpain activity had a marked increase ( P

Objective To investigate the effect of radiofrequency on trigeminal neuralgia as the chief complaint of intracranial benign tumor. Methods 18 patients with intracranial benign tumor mainly presenting with trigeminal neuralgia were treated with radiofrequency thermocoagulation guided by CT scanning. The numeric rating scales (NRS) of pain and the complication were observed. Results The scores of NRS decreased significantly after treatment (P<0.01). The common complications included facial numbness and masticatory movement obstacle. One patient recurred 13 months and another patient recurred 24 months after operation. Both were treated with radiofrequency once again, and the pain ceased. Conclusion Radiofrequency is effective on secondary trigeminal neuralgia after intracranial benign tumor.

Objective To observe the clinical effects of pulsed radiofrequency combined with acupuncture and traction on nerve-roottype cervical spondylopathy. Methods 62 patients with nerve-root type cervical spondylopathy were divided into treatment group (n=31),whom were treated with pulsed radiofrequency combined with acupuncture and traction, and the control group (n=31) only with acupunctureand traction. Results The incidence of effect was significantly higher in the treatment group than in the control group (P<0.01). ConclusionCombining with pulsed radiofrequency is more effective on nerve-root type cervical spondylopathy than acupuncture and traction only.

Objective To analyse the therapeutic effect and safety of radiofrequency thermocoagulation on refractory neuralgia after craniotomy.MethodsFourteen patients with refractory neuralgia after craniotomy were treated by radiofrequency thermocoagulation. The pain degree (Numeric Rating Scales, NRS) and the complication were observed.ResultsNRS decreased significantly after treatment(P<0.01). No serious complication was observed after radiofrequency thermocoagulation except numbness. No patients recurred after one year follow-up.ConclusionRadiofrequency thermocoagulation is effective on refractory neuralgia after craniotomy.

Objective To explore the effect of preoperative local blocking with compound lidocaine hydrochloride (CLH) on postoperative pain of patients undergoing craniotomy.Methods40 patients suffered from superatentorial occupying lesion and scheduled for craniotomy were randomly divided into the CLH group and control group with 20 cases in each group. In the CLH group, local blocking with CLH was performed just before craniotomy, while in control group local blocking with 0.5% procaine was performed preoperatively and all patients of two groups used patient-controlled intravenous analgesia (PCIA) with fentanyl postoperatively. Postoperative pain was assessed for 48 hours according to visual analogue scale (VAS), and the comparison of analgesia-related postoperative complications of the two groups was demonstrated.ResultsThere was no significant difference in VAS postoperatively and incidence rate of complications between two groups ( P>0.05).ConclusionCompound lidocaine hydrochloride local infiltration may provide a safe and effective analgesia for postoperative pain in patients after craniotomy.

Purpose To study the expression of TMPRSS2, ERG and ETV1 in prostatic cancer and their clinical pathologic signifi-cance. Methods Tissue microarray and immunohistochemistry (MaxVision) were used to detect TMPRSS2, ERG and ETV1 expres-sion in 70 prostatic cancer tissues, 10 prostatic intraepithelial neoplasia tissues and 18 benign prostate tissues. Results There was no statistical significance on positive rate of the expression of TMPRSS2 among prostatic cancer tissues, prostatic intraepithelial neoplasia tissues and benign prostate tissues (P>0. 05). The positive rate (81. 4%) of ERG in prostatic cancer tissues was significantly higher than that in prostatic intraepithelial neoplasia tissues ( 30. 0%) and benign prostate tissues ( 0. 0 ) ( P <0. 05 ) . The positive rate (68. 6%) of ETV1 in prostatic cancer tissues was significantly higher than that in prostatic intraepithelial neoplasia tissues (50. 0%) and benign prostate tissues (22. 2%) (P<0. 05). There was no correlation among the positive rates of TMPRSS2, ERG and ETV1 in prostatic cancer tissues and age (P>0. 05). The expression of TMPRSS2, ERG and ETV1 was positively correlated to Gleason score and clinical stage (P<0. 05). TMPRSS2 had positive correlation with ERG (rs =0. 465, P<0. 01). TMPRSS2 had positive correla-tion with ETV1 (rs =0. 590, P<0. 01). ERG had no positive correlation with ETV1 (rs =0. 151, P>0. 05). Conclusion ERG and ETV1 are expected to become therapeutic targets for prostate cancer. Detecting TMPRSS2, ERG and ETV1 at the same time is helpful to diagnosis and differential diagnosis of prostatic cancer, which might be new molecule markers of prostate cancer.