Objective To explore clinical effect of integrative medical therapy on infantile bronchiolitis. Methods 96 infants with bronchiolitis were randomly divided into a treatment group and a control group. The treatment group was treated with integrated traditional Chinese and western medicine, and the control group was treated with western medicine exclusively. Results The disappearance time for symptoms and signs in the treatment group was shorter than that of the control group. The hospitalized time (or time for observation of outpatient infusion) of the treatment group was also shorter than that of the control group (P＜0.05). The cure rate was 78.0% and the total effective rate was 96.0%% in the treatment group. However, the cure rate was 52.2% and the total effective rate was 69.6% in the control group, which had a significant difference in the two groups (P＜0.05). Conclusion Integrated traditional Chinese and western medicine can obviously shorten course of disease and hospitalized time, and also improve the curative effect of infantile bronchiolitis.

The bioterrorism-related anthrax, the general situation of anthrax prevalence in China and the latest advances in laboratory diagnosis of Bacillus anthracis were reviewed. The control and prevention measures on anthrax were also introduced and the measures responding to the possible bioterrorism-attack were proposed in this paper.

Objective To investigate the effect of sodium ozagrel injection on hemorheology, endothelin (ET) and nitrogen monoxidum (NO) of patients with non-ST segment elevation myocardial infarction (NSEMI). Methods A total of 81 NSEMI patients were divided randomly into two groups: control group and experimental group. Isosorbide mononitrate, low molecular heparin and simvastatin were administered in the control group while sodium ozagrel was added to the experimental group apart from the above-mentioned drugs. The clinical effect of sodium ozagrel was observed. The changes in the indexes of hemorheology, ET and NO were analyzed. Results The clinical effect was significantly better in the experimental group than in the control group (P<0.05). The level of ET was obviously lower after treatment than that in the experimental group and the control group before treatment (P<0.05). The level of NO was obviously higher after treatment than that in the experimental group and the control group before treatment (P<0.05). The changes of ET and NO in the experimental group post-treatment were more significant than those in the control group after-treatment (P<0.05). The level of hemorheology index after treatment was lower than that before treatment in the experimental group (P<0.05). High-shear rate of whole blood viscosity, platelet adherence rate, and fibrin as hemorheology indexes were lower after-treatment than those pretherapy in the control group (P<0.05). Conclusion Sodium ozagrel injection could affect hemorheology,ET and NO of patients with non-ST segment elevation myocardial infarction. Thefore, the therapeutic efficacy can be better if sodium ozagrel is added to the conventional therapy.

ObjectiveTo investigate the role of peroxisome proliferator-activated receptor gamma (PPARγ)/Caspase-8/Caspase-3 signaling pathway in the development of hyperlipidemia in rats.Methods 60 healthy male SD rats 〔4-week old,weight (110 ± 10) g〕 were randomly divided into control group,high-fat diet group,folic acid group,vitamin B12 group,folic acid and vitamin B12 group.After one week's feeding for adaptability,the groups of foloc acid,vitamine B12 and foloc acid + vitamineB12 were dealt with intraperitoneal injection of folic acid (0.5 mg/d),vitamin B12 (0.05mg/d),and folic acid (0.5 mg/d) plus vitamin B12 (0.05 mg/d),respectively,and fed with high fat diet simultaneously.Control group was dealt with intraperitoneal injection of normal saline (0.5 ml/d)and fed with normal diet.High-fat diet group was only fed with high fat diet.Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the mRNA level of PPARy,Caspase-8 and Caspase-3 in aorta abdominalis dissected at 17 weekends.Results Folic acid alone or jointed with vitamin B12 could effectively increase the level of PPARγ,while decrease the mRNA levels of Caspase8 and Caspase-3 as compared with high-fat diet group (P＜0.05),and folic acid plus vitamin B12 was more effective than folic acid alone (P＜0.05).Conclusions Folic acid alone or joined with vitamin B12 can improve the mRNA levels of PPARγ,Caspase-8 and Caspase-3 in vascular wall to protect endothelial injury from hyperlipidemia.

Objective To investigate the myocardial protection of adiponectin (ADP) /adiponectin receptor 1 (ADPR1) related-signal pathway in rats with limb ischemic preconditioning.Methods Thirty SD male rats were randomly divided into sham-operation group,myocardial ischemia reperfusion injury (MIRI) group,limb ischemic preconditioning (LIPC) group,LY294002 (the PI3-specific inhibitor) pretreatment group and LY294002+LIPC group (n=10 each).The mRNA level of myocardial ADP and ADPR1,the protein expressions of phosphatidylinositol 3-kinase (PI3k)phosphorylated Akt (p-Akt) were determined by RT-PCR and Western blot,respectively. Results As compared with sham-operation group,the mRNA levels of ADP and ADPR1 in MIRI group were significantly decreased (0.53 ± 0.07 vs.0.74 ± 0.08 and 0.52 ± 0.02 vs.0.72 ± 0.04,P＜0.05).Compared with MIRI group,the mRNA levels of ADP (0.72±0.21) and ADPRI (0.80±0.023) in LIPC group were increased,ADP(0.49±0.07) and ADPR1 (0.52± 0.02) mRNA were decreased in LY294002 group (both P＜0.05),but there were no difference in ADP(0.70±0.16) and ADPR1(0.78±0.05) mRNA between LY294002+LIPC group and MIRI group.The protein levels of Pl3k and p-Akt were lower in MIRI group than in sham-operation group (3.85±0.23 vs.2.83±0.22and 3.77±0.32 vs.2.66±0.29,P＜0.05).In contrast to MIRI group,the yield of PI3k (2.65±0.32)and p-Akt(2.26±0.27) protein (P＜0.05) were increased in LIPC group,but there were unproductive protein of PI3k (3.75 ± 0.65) and p-Akt (4.01 ± 0.71) in LY294002 group with no differences versus the levels of PI3k (3.23 ± 0.48) and p-Akt (3.17 ± 0.54) in LY294002 + LIPC group. Conclusions Limb ischemic preconditioning may protect myocardium by promoting serum adiponectin levels,improving myocardial mRNA expressions of ADP and ADPR1,activating the ADP/PI3k/Akt signaling pathway in reperfusion injury.

The results of this study showed that no deficiency of the spleen or kidney were seen in the normal control group animal. About 1/3 of the anemic -rat control group revealed, earlier or later, spleen deficiency, without kidney deficiency. For the group of rats with iron deficiency, deficiency of the kidney appeared after 17 days, with many rats suffered from spleen deficiency, in which 43 % of the animals changed from deficiency of the spleen to deficiency of both spleen -kidney or kidney deficiency alone. The difference between the iron -deficiency group and animic control group of spleen deficiency was that the serum iron was markedly lower in the former group, whereas the serum iron in the deficiency of both the kidney and spleen - kidney rats was even lower than the spleen deficiency group. When deficiency of the kidney was induced by iron deficiency, the abnormality in the membranous protein of RBC is even more serious. Conclusion: Iron might be one of the crucial biochemical basis of the relations between the kidney and spleen.

0 05) Conclusion Temperature and hemolysis affected the ECP level in different degrees But there was no significant influence of anti coagulation agent on the ECP level It could be concluded from our study that coagulation of the blood was not necessary to the ECP determination

Objective:To investigate the clinical effects of health and safety management in elderly patients receiving gastroenteroscopy under sedation.Methods:From February 2018 to November 2019, 126 elderly patients admitted to the cadre medicine and gerontology department of our hospital to undergo gastroenteroscopy under sedation were included as research subjects.According to the digital random number method, they were divided into the control group and the test group(n=63, each group). The control group was given routine management, while the test group was given routine management plus health and safety management.Clinical effects and safety were analyzed and compared between the two groups.Results:There was no significant difference in scores of the Hamilton anxiety scale(HAMA)and the Hamilton depression scale(HAMD)between the two groups before intervention.After intervention, the scores of HAMA and HAMD were lower in the test group than in the control group[(39.2±3.1)scores vs.(48.6±2.1)scores, (40.1±4.1)scores vs.(52.6±5.5)scores, t=19.926 and 14.463, both P=0.000]. The incidence of postoperative complications was lower in the test group than in the control group(7.94% or 5/63 vs.19.05% or 12/65, χ2=14.209, P=0.002). The differences between maximum and minimum values of heart rate(HR), diastolic blood pressure(DBP), respiratory rate(RR)and systolic blood pressure(SBP)were lower in the test group than in the control group(all P<0.05). The length of the procedure was shorter and the anesthetic dose was lower in the test group than in the control group(9.11±2.56 min vs.13.56±3.05 min, 15.35±2.66 ml vs.21.67±6.15 ml, t=8.870 and 7.486, both P=0.000). Patient satisfaction was higher in the test group than in the control group( χ2=16.383, P=0.014). Conclusions:Health and safety management can improve emotional health, stabilize vital signs and enhance satisfaction in elderly patients undergoing gastroenteroscopy under sedation.

Objective To investigate the diagnostic value of T-SPOT.TB in patients with fever.Methods T-SPOT.TB was carried out in 456 febrile patients who had no determined reason for fever or who could not be excluded from infection with Mycobacterium tuberculosis.These patients were followed up for their final clinical diagnosis and the diagnostic value of T-SPOT.TB was evaluated using the receiver operating characteristic (ROC) curve.Results In the present study,85 patients were clinically diagnosed with tuberculosis (TB) (including 34 cases of pulmonary TB,24 cases of TB infection defined as T-SPOT.TB positive without TB lesion,but effective with anti-TB treatment,10 cases of tuberculous pleurisy and 17 cases of other extrapulmonary TB) and 371 patients had no definite evidence of Mycobacterium tuberculosis infection (including 42 cases of suspected TB and 329 cases of other diseases).T-SPOT.TB had a high sensitivity of 84.3％ in TB patients and a high negative predictive value of 94.2 ％,but the specificity was 68.1％ and the positive predictive value was 41.4 ％.The Kappa value was 0.380 and the area under ROC curve was 0.762 (95％CI:0.707-0.817).T-SPOT.TB had a higher sensitivity in extrapulmonary TB than in pulmonary TB (88.2 ％ vs 78.1 ％),with the negative predictive values of 95.3 ％ and 92.6 ％,Kappa values of 0.438 and 0.299,and the areas under ROC of 0.791 (95 ％ CI:0.724-0.857) and 0.719 (95％CI:0.623-0.815),respectively.Ninety-nine cases were T-SPOT.TB positive out of 329 patients diagnosed with other diseases.Conclusions T-SPOT.TB test can be used as a method for TB diagnosis in patients without obvious TB lesions.Due to its relatively high negative predictive value,T-SPOT.TB can be used to rule out infection with Mycobacterium tuberculosis.