Objective To compare the effects between bloodletting at auricular points and acupuncture at specific points [Yintang (GV 29) or Yanglao (SI 6)] for analgesia in acute lumbar sprain.Method Totally 100 patients with acute lumbar sprain were randomized into a treatment group and a control group, 50 cases in each group. The treatment group was intervened by bloodletting at auricular points, while the control group was by acupuncture at specific points [Yintang (GV 29) or Yanglao (SI 6). The Present Pain Intensity (PPI) from the Short-form McGill Pain Questionnaire was taken as the observation index. The changes of PPI were compared respectively 10 min and 30 min after treatment.Result The PPI scores were significantly changed 30 min after treatment in both groups (P<0.05). The PPI scores in the treatment group were significantly different from that in the control group both 10 min and 30 min after intervention (P<0.05).Conclusion Bloodletting at auricular points and acupuncture at specific points are both effective in releasing pain in acute lumbar sprain, while bloodletting at auricular points can produce a more significant real-time analgesic effect.

The bioterrorism-related anthrax, the general situation of anthrax prevalence in China and the latest advances in laboratory diagnosis of Bacillus anthracis were reviewed. The control and prevention measures on anthrax were also introduced and the measures responding to the possible bioterrorism-attack were proposed in this paper.

Objective: To investigate the factors affecting spontaneous preterm birth in singleton pregnancy, so as to provide insights into reducing the risk of preterm birth. Methods: A total of 230 lying-in women with spontaneous preterm birth in singleton pregnancies admitted to the Hangzhou Obstetrics and Gynecology Hospital were selected as the case group, and lying-in women with full-term birth in singleton pregnancies in the hospital during the study period were selected as controls. Basic information and pregnancy status were collected through the hospital information system, and factors affecting spontaneous preterm birth in singleton pregnancies were analyzed using a multivariable logistic regression model. Results: The lying-in women in the case group had a mean age of (33.40±3.16) years and a mean gestational weeks at delivery of (34.72±2.15) weeks. The lying-in women in the control group had a mean age of (28.30±3.92) years and a mean gestational weeks at delivery of (39.84±2.09) weeks. Multivariable logistic regression analysis identified age of 35 years and older (OR=1.280, 95%CI: 1.022-1.603), induced abortion three times and above (OR=3.122, 95%CI: 1.368-7.121), history of preterm birth (OR=3.769, 95%CI: 1.725-8.240), premature rupture of membranes (OR=12.708, 95%CI: 4.836-33.391), gestational hypertension (OR=2.934, 95%CI: 1.313-6.550), gestational diabetes (OR=2.510, 95%CI: 1.249-5.038) and cervical canal length ≤25 mm (OR=5.832, 95%CI: 2.380-14.279) as factors affecting spontaneous preterm birth in singleton pregnancies. Conclusion Spontaneous preterm birth in singleton pregnancy may be associated with age, number of induced abortions, premature rupture of membranes, complications in pregnancy and cervical canal length.

OBJECTIVE:To establish a method for the limit detection of aconitine in Wuqi shujin tongluo tablet. METHODS:TLC was conducted to identify the aconitine;TLC plate was silica gel G plate,developing solvent was toluene-ethyl acetate-chloro-form-acetone-ammonia(20:18:3:6:1,V/V/V/V/V),chromogenic agent was bismuth potassium iodide test solution and sodium ni-trite ethanol test solution;and durability investigation and detection limit detection were used to optimize the TLC plate,tempera-ture and humidity. RESULTS:TLC showed the aconitine had clear spots and negative control without interference. The durability was good;detection limit was 0.9 μg;available TLC plate was Merck HPTLC prefabricated plate, silica gel G TLC plate setf-made silica gel G TLC plate with adhesive of sodium carboxymethycellulose;temperature was 5-16 ℃ and humidity was 32%-72%. CONCLUSIONS:The method is simple and reproducibility,and can be used for the limit detection of aconitine in Wuqi shujin tongluo tablet.

Objective To construct the multi-epitope antigen(mea) gene of G2 glycoprotein of Hantavirus SEO type L99 strain.Methods The B cell epitopes was chosen and connected by three-peptide GPG after the amino acid sequence of G2 protein was analyzed and predicted by bioinformatical soft wares.The corresponding gene mea was constructed by overlap PCR,and cloned into the prokaryotic expression plasmid pET32a(+).Results The mea gene was successfully constructed by the five epitopes being chosen.The recombinant plasmid pET32a-mea was acquired by directional cloning.Conclusion The mea and its expression system E.coli BL21/pET32a-mea were constructed for the first time.The foundation was laid for the expression of the mea and its immunological applications.

Objective To study a rapid and sensitive method for determination of virulence-associated genes in O1El Tor,O139,non-O1/non-O139Vibrio cholerae strains.Methods Five pairs of primers were designed respectively ac-cording to cholera toxin sub-unit A gene(ctxA),accessory cholera enterotoxin gene(ace),zonula occludens toxin gene(zot),toxin coregulated pilus A gene(tcpA)and toxR regulatory protein gene(toxR).Multiplex PCR(MPCR)procedures for simultaneously detecting those five genes were established.The gene information of the virulence-associated genes in the Vibrio cholerae strains was obtained through agar gel electrophoresis for products of single amplification of the MPCR.Results The five virulence-associated genes in the positive control Vibrio cholerae O139(MO45strain)could be detected and the results were correct,which could meet the designed request for the method.In the other tested strains(O1EL Tor,O139,non-O1/non-O139)could be detected1to5kinds of the virulence-associated genes.Based on the results of the variety of carried virulence-associated genes,the tested Vibrio cholerae strains could be classified as5kinds of genetypes,and the Vibrio cholerae could be distinguished between toxic and non-toxic strains.The sensitivity of the MPCR approach reached to10 2 cfu/ml.Conclusion This method is rapid,specific and sensitive,which possess great value for practical application.

Objective To screen the high-efficiency predominant bacteria which can decompound 2-mercaptobenzothiazole, the accelerant for producing latex, in the organic wastewater. Methods Sampling from manufacturing environment, we got the predominant bacteria by primary screening, isolating and functional tests, and performed simulated test ground decompounding tests by using all bacteria. The enrichment of the predominant bacteria was followed by screen and identification to select the high-efficiency bacteria. Results 75 strains of predominant bacteria were obtained by primary screening. The simulated decompounding tests were performed after the mixed bacteria were tamed. The ratio of elimination for chemical oxygen demand (COD) was about 60.8%-97.7%, and the average was 77.2%. The predominant bacteria adhered to the surface of the active carbon, the carrier, and formed the biological film. Through screening and identification the Bacillus cereus showed to be predominant (90%). Conclusion The technology of high-efficiency predominant bacteria can be used for decompounding 2-mercaptobenzothiazole in the organic wastewater.

Objective:To investigate the clinical effects of health and safety management in elderly patients receiving gastroenteroscopy under sedation.Methods:From February 2018 to November 2019, 126 elderly patients admitted to the cadre medicine and gerontology department of our hospital to undergo gastroenteroscopy under sedation were included as research subjects.According to the digital random number method, they were divided into the control group and the test group(n=63, each group). The control group was given routine management, while the test group was given routine management plus health and safety management.Clinical effects and safety were analyzed and compared between the two groups.Results:There was no significant difference in scores of the Hamilton anxiety scale(HAMA)and the Hamilton depression scale(HAMD)between the two groups before intervention.After intervention, the scores of HAMA and HAMD were lower in the test group than in the control group[(39.2±3.1)scores vs.(48.6±2.1)scores, (40.1±4.1)scores vs.(52.6±5.5)scores, t=19.926 and 14.463, both P=0.000]. The incidence of postoperative complications was lower in the test group than in the control group(7.94% or 5/63 vs.19.05% or 12/65, χ2=14.209, P=0.002). The differences between maximum and minimum values of heart rate(HR), diastolic blood pressure(DBP), respiratory rate(RR)and systolic blood pressure(SBP)were lower in the test group than in the control group(all P<0.05). The length of the procedure was shorter and the anesthetic dose was lower in the test group than in the control group(9.11±2.56 min vs.13.56±3.05 min, 15.35±2.66 ml vs.21.67±6.15 ml, t=8.870 and 7.486, both P=0.000). Patient satisfaction was higher in the test group than in the control group( χ2=16.383, P=0.014). Conclusions:Health and safety management can improve emotional health, stabilize vital signs and enhance satisfaction in elderly patients undergoing gastroenteroscopy under sedation.

Objective Signal transducers and activators of transcription 3 (STAT3) silenced by RNA interference (RNAi) technique were used to induce the apoptosis and growth inhibition in T24 and 5637 bladder cancer cells. Methods Three recombinant plasmids pGenesil-1-shRNA-STAT3 was constructed and transfected into T24 and 5637 cells. The expression of STAT3 gene was detected by RT-PCR and Western blotting. FCM was used to observe the apoptosis in T24 and 5637 cells. Results pGenesil-1-shRNA-STAT3 was successfully constructed, and transfected into T24 and 5637 cells. RT-PCR and Western blot analysis demonstrated that pGenesil-1-shRNA-STAT3 could significantly inhibit the expression of STAT3 in T24 and 5637 cells; FCM results show that it could suppress the growth of 1'24 and 5637 cells. Conclusion pGeneSiI-1-shRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of T24 and 5637 cells.

Objective To discuss the clinical value and nursing of the three-endoscope in the treatment of eholedoeholithiasis. Methods 45 eases of choledocholithiasis patients who were treated with LCDE (three-endoscope) were named as the research group.56 patients who received traditional open ab-dominal surgery were set as the control group. The average hospitalization time and satisfaction degree with nursing were compared, t test and χ2 test were adopted. Results The average hospitalization time was shorter and satisfaction degree with nursing was higher in the research group than those in the control group. Conclusions The treatment of choledochohthiasis with three-endoscope is safe and feasible, es-pecially when combined with antibiotics lavage and stone dissolution through naso-biliary duct.The opera-tion can widen the surgical indication,reduce the risk of surgery with little damage,clear stones completely, reduce postoperative complicatioas,make patients recover faster, shorten the hospital stay and achieve the same or better treatment results when compared to the traditional open abdominal surgery.