Objective To investigate the clinical value of polyene phosphatidyl choline combined with lifestyle intervention in the treatment of non-alcoholic fatty liver.Methods A total of 120 cases of non-alcoholic fatty liver patients were divided into two groups by random number table method with 60 cases in each,control group was given the liver lipid lowering drugs,lifestyle and other conventional treatment,observation group was treated with polyene phosphatidyl choline on the basis of the control group,treatment for 3 months,the curative effect in two groups was compared.Results The triglyceride (TG),total cholesterol (T C),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C),alanine aminotransferase (ALT),aspartate aminotransferase (AST) and gamma glutamine transferase (GGT) after treatment in two groups were significantly improved than those before treatment [contwl group:(2.4 ±0.6) mmol/L vs.(3.3 ± 1.3) mmol/L,(4.4 ± 1.4) mmol/L vs.(2.2 ± 1.0) mmol/L,(3.0 ± 0.5) mmol/L vs.(3.5 ± 0.8) mmol/L,(1.3 ± 0.6) mmol/L vs.(0.9 ± 0.5) mmol/L,(42.3 ± 11.5) U/L vs.(109.7 ± 36.8) U/L,(40.8 ±7.4) U/L vs.(49.8 ±9.8) U/L,(54.2 ± 12.3) U/L vs.(63.4± 15.7) U/L; observation group:(1.1 ± 0.4) mmol/L vs.(3.3 ± 1.1) mmol/L,(6.1 ± 1.5) mmol/L vs.(2.3 ± 1.1) mmol/L,(2.6 ± 0.4) mmol/L vs.(3.6 ± 0.9) mmol/L,(1.7 ± 0.7) mmol/L vs.(0.9 ± 0.6) mmol/L,(78.6 ± 19.8) U/L vs.(112.1 ±41.1) U/L,(30.2 ±7.8) U/L vs.(51.2 ± 11.3) U/L,(42.7 ±9.8) U/L vs.(66.8 ± 14.6) U/L],and the differences were statistically significant (P ＜ 0.05),and observation group was better than control group,and the differences were statistically significant (P ＜ 0.05).The total effective rate in observation group was higher than that in control group [93.3％(56/60) vs.76.7％(46/60)],and the difference was statistically significant (P ＜ 0.05).Compared with before treatment,the physical health,mental health,social health in two groups were significantly improved [control group:(135.6 ± 21.2) scores vs.(127.8 ± 18.9) score,(121.9 ± 16.9) scores vs.(110.2 ± 13.6) scores,(85.8 ± 12.1) scores vs.(78.9 ± 11.1) scores;observation group:(149.4 ±20.2) scores vs.(128.1 ±21.9) scores,(142.3 ±23.1) scores vs.(108.9 ± 12.8) scores,(94.1 ± 15.3) scores vs.(80.2 ± 13.5) scores],and the differences were statistically significant (P ＜0.05),and observation group was better than control group,and the difference was statistically significant (P ＜0.05).Conclusions Polyene phosphatidyl choline combined with lifestyle intervention in treatment of non-alcoholic fatty liver has obvious curative effect,which can significantly improve the liver function and improve the quality of life of patients,it is safe and effective method in treatment of patients with non-alcoholic fatty liver and worthy clinical promotion.

Objective To research the relationship between Helicobacter pylori infection,nonsteroidal anti-inflammatory drugs (NSAIDs) and peptic ulcer combined with bleeding.Methods The clinical data of 180 peptic ulcer patients from January 2012 to June 2013 were analyzed retrospectively,and they were divided into bleeding group (75 patients) and non-bleeding group (105 patients).The risk factors of bleeding were analyzed.Results The single factor analysis showed that age ≥60 years,history of alimentary tract hemorrhage,cardiovascular and cerebrovascular diseases,and simple caplendused NSAIDs were the risk factors of peptic ulcer combined with bleeding (P ＜ 0.01).Multiple factors Logistic regression analysis showed that age ≥60 years [OR(95％ CI):0.9(0.5-1.7),P=0.003],history of peptic ulcer [OR (95％ CI):1.4 (0.7-2.6),P =0.006],history of alimentary tract hemorrhage [OR (95％ CI):2.1 (1.4-3.0),P=0.007],single Helicobacter pylori infection [OR (95％ CI):3.2(2.1-4.2),P=0.001],history of cardiovascular and cerebrovascular diseases [OR (95％ CI):1.3 (0.5-1.9),P =0.005],simple caplendused NSAIDs [OR (95％ CI):2.4 (1.6-3.5),P =0.001] were independent risk factors of peptic ulcer combined with bleeding.Helicobacter pylori infection combined with NSAIDs could improve the incidence rate of peptic ulcer combined with bleeding(P ＜ 0.01).Conclusion Helicobacter pylori infection combined with NSAIDs can improve the incidence rate and risk of peptic ulcer combined with bleeding.

Aim: The tandem Claisen rearrangement/Diels-Alder reaction in ionic liquid was carried out to find better changes for the conversion. Methods: The synthesis started with replaced benzoic acid via acylation, cycli-zation, demethylation, allylation and then tandem Claisen rearrangement/Diels-Alder reaction in trational solvent and ionic liquid separately. Results: BmimBF_4 raised the yield of the target compound and shortened the reaction time compared with the traditional solvent Conclusion: BmimBF_4 can promote the tandem Claisen rearrange-ment/Diels-Alder reaction.

Objective To know the usage of Chinese patent medicine of western medicine doctors in general hospitals. Methods By means of informal discussion with experts, present situation was known; By means of screening questions in questionnaire, nationwide investigation was conducted by this questionnaire about Chinese patent medicine and the results were examined by statistical analysis. Results That the abuse of Chinese patent medicine by western medical doctors was quite common. The overall quality of their prescriptions was not good. Most of the doctors have never received specialized continuing education of traditional Chinese medicine, only having preliminary knowledge or nothing on syndrome differentiation and treatment of traditional Chinese medicine. Conclusion The management department should take following measures: further enhance continuing education of TCM;formulate related policies;improve present situation.

Aim To investigate the effect of salvianolate syophilized injection on brain tissue gene expression profiles in stroke of diabetic rats.Methods T1DM was induced in adult male Wistar rats by injecting streptozotocin.T1DM rats were then subjected to 90 minutes of middle cerebral artery occlusion(MCAO).The rats were randomly assigned to sham group(DM+Sham),ischemia-reperfusion group(DM+ MCAO/R),edaravone group(6 mg·kg-1,ED)and salvianolate lyophilized injection treatment group(5.25,10.5,21 mg·kg-1,SLI)with 13 rats in each group.Drugs were administered by tail vein injection 3 hours after MCAO/R,daily and lasting for 14 days.Infarct volume and gene expression in the brain tissue were detected by TTC staining and the gene chip technique.Results Compared with DM+Sham group,67 differential expressed genes were detected in the DM+MCAO/R group,among which 41 genes were up-regulated and 26 genes were down-regulated.Compared with DM+MCAO/R group,59 differential expressed genes were detected in the SLI(21 mg·kg-1)group,among which 45 genes were up-regulated and 14 genes were down-regulated.Hierarchical cluster results suggested that a number of genes were significantly changed in T1DM rats,such as Ly6i,Pax7 and Irx2.Effects of SLI on the stroke in T1DM rats were majorly related to coagulation and hemostasis system,inflammatory cytokines,oxidative stress,substance metabolism,angiogenesis and signal transduction.Conclusion Salvianolate lyophilized injection protects against focal cerebral ischemia/reperfusion injury in type 1 diabetic rats through regulation of the coagulation and hemostasis system,inflammatory cytokines,oxidative stress,substance metabolism,angiogenesis and signal transduction.

Objective To explore the effects of family empowerment model on medication adherence in youth patient with cerebral infarction.Methods By convenience sampling,a questionnaire survey was conducted among 84 patients and families of neurology department in a third-grade and a-level general hospital in Zhengzhou.The patients and families were randomly divided into the control group and the intervention group,each group consisted of 42 patients and families.Patients and families in the control group got routine health education,but patients and families in the intervention group were intervened under family empowerment.The medication adherence of the patients were compared between the two groups before and after health education.Results The medication adherence in both groups were higher after health education than that before health education,the diffidence in the comparison of medication adherence was statistically significant between the two groups after health education.Conclusions The family empowerment model can improve the medication adherence in youth patient with cerebral infarction.

Objective To investigate the feature of Th1 cytokines induced by Mtb-specific antigen in patients with refractory pulmonary tuberculosis.Methods 28 patients with refractory pulmonary tuberculosis,67 patients with first-treated pulmonary tuberculosis,and 25 healthy controls with positive T.spot (LTBI group) were enrolled.IFN-γ,IL-2 and TNF-α in supernatants from PBMCs stimulated with ESAT-6 and CFP-10 were analyzed with Bender Flowcytomix on flow cytometry.Results The levels of the three cytokines were utmost high in patients with first-treated pulmonary tuberculosis.The lowest level of IFN-γ and IL-2 were induced in patients with refractory pulmonary tuberculosis,and were significantly lower than the LTBI group(Mann-Whitney U ＝ 105.5,162.5,P ＜ 0.01).Conclusions The immunotherapy with IFN-γ and IL-2 may play a role in treatment for refractory pulmonary tuberculosis but not for most of first-treated pulmonary tuberculosis.

Objective To translate the Work Limitations Questionnaire and examine its reliability and validity,and explore its applicability at home.Methods 300 nurses were surveyed with the Chinese version of the Work Limitations Questionnaire by convenience sampling; and internal consistency reliability,test-retest reliability,construct validity and criterion validity were applied to evaluate the questionnaire.Results The Cronbach α coefficients of the time managing scale,physical scale,mental-interpersonal scale,output scale and total questionnaire were 0.882,0.864,0.913,0.839 and 0.922 respectively;and their test-retest reliability coefficients were 0.857,0.837,0.871,0.803 and 0.893 respectively.Four common factors were abstracted by using factor analysis,with the cumulative variance contribution rate at 61.293％.Using SF-36 as an external criterion,the correlation coefficients of the WLQ scores with the physical function dimension and mental health dimension of the SF-36 were-0.24 and-0.33.Conclusions The reliability and validity of the questionnaire is reasonable.So this questionnaire can be used to measure employee's presenteeism.

This study was aimed to confirm animal model and evaluating indicators of the chronic obstructive pulmonary disease combined with pulmonary interstitial fibrosis (PIF-COPD). PIF-COPD rats were induced by intratracheal administration of Bleomycin(BLM, 6 mg·kg-1) and cigarette smoke stimulation for 47 days. After that, the lung function and index were measured. Changes of pathomorphism of lung were observed with hematoxylin-eosin staining. Collagen protein I and III were determined by Sirius red staining. The protein antibody microarray chip was employed for serum cytokine expression pattern measurement. The results showed that in the lung function testing, the central airway resistance, tissue damping, dynamic elasticity of model rats increased significantly and the dynamic compliance reduced significantly. The lung index of model rats increased significantly (compared with the control group, P < 0.01). The pathological tests showed that lung tissues of model rats showed emphysema and fibration with inflammatory infiltration, and content of collagen type I and III increasing significantly. The contents of MMP-8, TIMP-1, IL-10, IL-13, PDGF-AA, Beta-NGF of model rats serum increased obviously compared with the control group and RAGE reduced compared with the normal control. It was concluded that rats which were induced by intratracheal administration of BLM (6 mg·kg-1) and cigarette smoke stimulation for 47 days can be used as animal model establishment of PIF-COPD. The respiratory function and serum levels of MMP-8, TIMP-1, IL-13, PDGF and NGF can be used as evaluating indicators of PIF-COPD model.

BACKGROUND: Carboxymethyl chitosan is a water-soluble derivate modified from chitosan, with various biological activities. It is a good ligand of metal ion and can integrate Ca~(2+) to prepare a novel biological material. OBJECTIVE: To explore a method for preparing carboxymethyl chitosan calcium (CCC) and analyze its properties and structure. METHODS: CCC was produced by carboxymethyl ohitosan reacting with solution of calcium chloride. The solubility, carboxymethylation degree, rotational viscosity, and calcium content of CCC were determined, and infrared and ultraviolet spectral analyses were performed.RESULTS AND CONCLUSION: The calcium content of CCC was approximately 15%. Compared with carboxymethyl chitosan, infrared spectrum and ultraviolet spectrum of CCC were changed. The prepared CCC is a new calcium compound through property and structural analysis.