Objectives: To clarify the antiarrhythmia mechanism of matrine by observing its effects on intracellular calcium concentration in cultured myocardial cells of SD rats. Methods: Myocardial cells of newborn(sucking) rats were fed with culture medium supplemented with verapamil、 potassium chloride and different dose of matrine, respectively. By means of the image analysis system, intensity changes of intracellular calcium were determined with the fluorescent probe to estimate indirectly inward calcium influx under the influence of matrine. Results: It was found that high potassium caused an increase in fluorescent intensity of intracellular calcium which could be inhibited by matrine. Conclusion: It is suggested that matrine may be a kind of calcium antagonium producing its antiarrhythmic action.

DY-1 suspending rescue seat on helicopter is a helicopter-equipped apparatus which is used to rescue flight personnel forced to parachute or landing,or other individual disasters. The thesis briefly introduces the seal s working principle,performances and some experimenting results. It errphasizes on recounting the structure and the condition at operation of the active arm's locking mechanism of the rescue seat's peron fixed ring.

In 2015 , policy reforms on centralized drug purchasing system have been released one by one , and the quality of medicines has been highly valued .Centralized drug purchasing system distinguishes different qualities of drugs mainly through bidding grouping , in order to achieve “uality priority”.However , there are yet some existing problems in group bidding policy at present , such as lack of a unified hierarchy , no scientific indicators and so on , which inevitably weaken the leading role of centralized purchasing policy over the quality of medicines .This paper , focuses on the study of group bidding , and the existing main problems are found through the statistical analysis of rel-evant policies introduced in different provinces .Finally, it explores some optimization strategies for “Group Bidding”which is of paramount importance .

In the progress of the new healthcare reform, China has carried out a series of reform exploration in the drug distribution system.But fluctuating markups of drugs are still the main cause of high drug price.In order to simplify the distribution procedure, the National Health and Family Planning Commission proposed to encourage the implementation of two-receipt system nationwide.As the important part of the price system of drug circulation, drug distribution system made a great impact on the implementation of two-receipt system policy.Focusing on two provinces namely Fujian and Shaanxi provinces, this paper will discuss the typical drug distribution system in china.This paper has evaluated the main body and operation pattern in the implementation of two-receipt system, and by analyzing the benefits and drawbacks, in the same provinces.It has put forward the optimized distribution mode and provided policy suggestions for strengthening the two-receipt distribution system.

It expounded the important position of physique in etiology and process of disease,investigated essence and application of physique in disease control,revealed the classifi cation of physique in three yin three yang.Also it expounded the signif icance of diagnosis of disease in TCM theory and clinical therapy,then revealed basic pathogenesis of diabetes:damaged yin and qi due to inner heat.Then it discussed the syndrome differentiation in the TCM treatment and suggested that it should combine with physique differentiation and diagnosis of disease.At last it expounded the trinity of physique differentiation,diagnosis of disease and syndrome differentiation in treating diabetes and its complication.

To summarize current pathogenesis theories of diabetic nephropathy,focusing on kidney theory,spleen theory,collateral disease theory,toxicity injuring kidney meridian collaterals theory and micro-zhengjia theory.As for the study development,new research idea has been put forwards which highlights basic TCM syndrome study,clinical trial and experimental study,as it will improve the research works in this field.

Being introduced in Korean peninsula, Chinese medicine, which is called Korean medicine in Korea, has caused great influence in Korean ethnomedicine, culture and living.Korean medicine takes an important part in Korean medical education and medical treatment. This paper introduces the origin of Korean medicine, the current mode and feature of Korean medicine education, as well as the overview and characteristic of Korean Hospital Affiliated Qing Xi University and Korean Hospital Affiliated Daejeon University. The mode of Korean medical education and hospital can offer reference for Chinese medicine education.

BACKGROUND:Primary human lung epithelial cel s are difficult to be isolated and cultured in vitro, which is characterized as limited sources, low cel viability, slow proliferation capacity, and lacking of differentiation capability.
OBJECTIVE:To establish an air-liquid interface model of lung epithelium by in vitro proliferation of human bronchiolar epithelial cel s, which is used for research on function of lung epithelial cel s.
METHODS:Primary human bronchiolar epithelial cel s were isolated using Pronase and DNase I combined digestive methods, and then proliferated using medium containing ROCK kinase inhibitor. The proliferated cel s were used for establishment of the air-liquid interface epithelium model. Cel differentiation was identified using scanning electron microscope, phase contrast microscope and immunofluorescent staining.
RESULTS AND CONCLUSION:The primary human bronchiolar epithelial cel s could be expanded successful y using medium containing ROCK kinase inhibitor, and the basal cel marker Cytokeratin14 was preferential y expressed in the proliferated cel population, indicating that these basal cel s might be the main subpopulation of human lung epithelial stem cel s. Subsequently, the proliferated cel s under the air-liquid interface could differentiate into ciliated cel s and non-ciliated column cel s. The results suggest that the proliferation and differentiation of human bronchiolar epithelial cel s were maintained in the presence of ROCK kinase inhibitor, and the air-liquid interface could promote the differentiation of human bronchiolar epithelial cel s.

Objective:To implement rapid acquisition gradient echo(RAGE)pulse sequence in clinical MRI scaner.Methods:Pascal language is engaged to edit source code.Number of slice excited,order of phase encoded,phase recycle of RF pulse,NEX,on/off of the gradients,and so on are all controlled by sequence parameters.SI×N_(phase)×N_(slice) data of 32 bctys were allocated to restore the k space.Source code of sequence was compiled with executable file and is loaded to RINMR software.Image of human brain are acquired.The experiment has been conducted on 0.36T permanent MRI system.Resuits:In the case of 256×256 matrix acquisition,time of single slice is 4 seconds.The resolution and SNR of the image are all adquately satisfy the clinical application.

Objective To evaluate the effects of OPRM1All8G and CYP3A4*18B genetic polymorphism and the interaction on postoperative analgesia with fentanyl in the patients undergoing radical resection of lung cancer.Methods One hundred and thirty-nine patients (native of Henan province),aged 40-64 yr,weighing 40-70 kg,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,scheduled for elective radical resection of lung cancer under general anesthesia,were enrolled in this study.The polymorphic sites of the OPRM1All8G and CYP3A4*18B allele were analyzed by using polymerase chain reaction technique and ABI 3130 Genetic Analyzer.The patients were divided into wild homozygote group (group AA,group *1/*1),heterozygote group (group AG,group * 1/*18B) and mutation homozygote group (group GG,group *18B/*1SB) according to their genotypes.The patients were divided into 7 groups according to the interaction between the two genes:AA plus *1/*1 group (group Ⅰ),AA plus *1/*18B group (group Ⅱ),AG plus *1/*1 group (group Ⅲ),AG plus *1/*18B group (group Ⅳ),GG plus * 1/*1 group (group Ⅴ),GG plus *1/*18B group (group Ⅵ) and *18B/*18B group (group Ⅶ).Patientcontrolled intravenous analgesia with fentanyl was started at the end of surgery to maintain the visual analogue scale ≤ 3 points.The amount of fentanyl used within 24 and 48 h after surgery was recorded,and the occurrence of adverse reactions within 48 h after surgery was observed.Results The amount of fentanyl used within 24 and 48 h after surgery was significantly higher in group GG than in group AA (P＜0.05).The amount of fentanyl used within 48 h after surgery was significantly lower in group *18B/*18B than in group *1/*1 (P＜0.05).The amount of fentanyl used within 48 h after surgery was significantly higher in Ⅱ and Ⅳ groups than in group Ⅰ,in group Ⅲ than in group Ⅱ,in group Ⅴ than in Ⅰ-Ⅳ groups,and in group Ⅵ than in Ⅱ and Ⅳ groups,and was significantly lower in group Ⅶ than in Ⅰ-Ⅵ groups (P＜ 0.05).There was no significant difference in the incidence of adverse reactions within 48 h after surgery between groups (P＞0.05).Conclusion OPRM1A1l8G and CYP3A4*18B genetic polymorphism and the interaction are the genetic factors contributing to individual variation in fentanyl pharmacodynamics in the patients undergoing radical resection of lung cancer.