Objective To study the effect of Busui Jiannao Prescription on ethology, GSH content and GSH-PX activity in brain of PD model rats. Methods Forty-five the aging pure C57BL rats were divided into a negative injury group, a model group, and a Chinese medicine group. PD animal model were established by injection with MPTP and were treated with Busui Jiannao Prescription by intragastric administration. Score changes of PD model rats in the test of pole-climbing and suspending were observed. GSH content and GSH-PX activity were further measured. Results After treating with Busui Jiannao Prescription, the test scores in pole-climbing and suspending were improved, GSH content and GSH-PX activity in substantia nigra were increased. Conclusion Busui Jiannao Prescription can regulate the behavior coordination and repair the damage of dopaminergic neurons in the substantia nigra of PD model rats.