Objective To evaluate magnifying Fuji intelligent chromo endoscopy (FICE) in diagnosis of colorectal lesions,and to explore the correlation between pit pattern,expression of Angiopeietin-2 (Ang-2) and mierovessel density (MVD). Methods A total of 100 colorectal lesions with pit patterns ranging from type Ⅰ to type Ⅴ (20 cases in each type) determined by magnifying FICE were divided into group A (n = 40,type Ⅰ and Ⅲ ),group B (n = 40,type Ⅲ and Ⅳ ) and group C ( n = 20,type Ⅴ ). The resuits of FICE were compared with pathological findings. Expression of Ang-2 was examined by immunohistochemical streptavidin-perosidase method and MVD was calculated. The correlation between pit pattern,Ang2 expression and MVD was analyzed. Results The diagnostic sensitivity,specificity and consistent rates of magnifying FICE for non-neoplastic colorectal lesions were 88.0%,92. 5% and 90. 2%,respectively,and those for neoplastic lesions were 94. 8%,91.7% and 93. 2%,respectively,with an overall consistent rate for colorectal lesions at 92. 0%. The positive expression rate of Ang-2 and MVD were progressively increasing from group A,B to C. Conclusion Magnifying endoscopy with FICE is valuable to differentiate neoplastic colorectal lesions from non-neoplastic ones. The positive expression of Ang-2 and MVD are closely correlated with the pit patterns of colorectal lesions.

Objective To explore the differentiation potency of ES cell-derived epidermal stem cells compounded by dermal analogs reconstructed with bone MSCs in hypodermis.Methods The dermal analogs were reconstructed with rat bone MSCs and compound gel-gelatin sponge.E14-ES cells,labeled with Hoechst 33342,were cocultured with human amnion.Four days later,epidermal stem cell clones were formed.The skin analogs,reconstructed with ES cell-derived epidermal stem cells and dermal analogs,were transplanted into 129 mice hypodermis.The differentiation tissue of skin analogs was sampled at 2,4,6,8 weeks.The sections were observed with HE staining,immunohistochemical and di-labeled immunofluorescence methods to test the expression of ?1 integrin,CK15,CK19,CEA,CK18.Results The sections were showed tubular or follicular like structures formed with simple or stratified epithelium at 2 and 4 weeks.Keratinized stratified squamous epithelium,sweat glands-like,sebaceous glands-like and hair follicles-like structures were observed at 6 week and 8 week after transplantation.The cells labeled by Hoechst 33342,formed tubular or follicular like structures,expressed?1 integrin,CK15,CK19,CEA and CK18positive respectively at 2 and 4 weeks.The sweat glands-like structure expressed CEA and CK18 positive respectively at 6 and 8 weeks.There were more sebaceous glands-like structures.Conclusion ES cell-derived epidermal stem cells compounded by dermal analogs reconstructed with bone MSCs can differentiate into keratinized stratified squamous epithelium,sweat glands-like,sebaceous glands-like and hair follicles-like structures in hypodermis.

Objective To provide a new way for treatment of full thickness skin defects by embryonic stem(ES) cell-derived epidermal-like stem cells. Methods Epidermal like stem cells,labeled by Hoechst 33342 and carried by a layer of biomembrane,were transplanted into the defective skin of mice.The differentiation tissue of donor cells was sampled each week.The sections were observed with HE staining,immunohistochemical and di-labeled immunofluorescence methods to test the expression of ?1 integrin,CK15,CK19,CK10,CEA. Results The full thickness skin defects were healed in 2 weeks.The newborn skin was thicker than the normal skin.The basal layer cells proliferated.There were more bulky cellular poles towards dermis.The cells labeled by Hoechst 33342,located in the newborn epidermis and tubular or follicular structures in dermis,expressed ?1 integrin and CK15 positive respectively in the first 3 weeks.There were sweat gland-like,sebaceous gland-like and hair follicle-like structures in the newborn dermis after 4 weeks.Basal cells of keratinized stratified squamous epithelium expressed CK19 and CK10 positive respectively and sweat gland-like structure expressed CEA positive.Conclusion ES cell-derived epidermal stem cells can restore mice full thickness skin defects.There are epidermis,sweat gland-like,sebaceous gland-like and hair follicle-like structures in the newborn skin.

Objective To investigate the effect of Hedan tablet combined with fluvastatin on serum LDL and heart rate variability in patients with coronary atherosclerotic heart disease (CHD).Methods 110 patients with coronary heart disease who were treated from March 2015 to June 2016 in our hospital were selected and randomly divided into observation group and control group, with 55 cases in each group.The patients in the observation group were treated with Hedan tablet combined with fluvastatin, and the control group was treated with fluvastatin.The changes of heart rate variability, blood lipids and inflammatory factors were compared before and after treatment, and the clinical curative effect was observed.Results After treatment, the total effective rate of the observation group was 96.4%, significantly higher than the control group 85.5%, the difference was statistically significant (P<0.05).The SDNN of the observation group was (85.42 ±9.11) ms and the SDANN was (49.11 ±5.13) ms, was significantly higher than those in the control group (76.87 ±8.12) ms, (44.16 ±4.76) ms.In the observation group, TC was (4.14 ±0.45) mmol/L, TG was (1.26 ±0.16) mmol/L, LDL was (2.08 ±0.31) mmol/L, significantly lower than the control group (4.78 ±0.51) mmol/L, (1.42 ±0.18) mmol/L, (2.43 ± 0.27) mmol/L, and HDL levels was (1.51 ±0.18) mmol/L, significantly higher than those in the control group (1.35 ±0.15) mmol/L, the difference was statistically significant (P<0.05), and the levels of inflammatory factors in the observation group were significantly lower than those in the control group, the difference was statistically significant (P<0.05).Conclusion Hedan tablets combined with fluvastatin in treating coronary atherosclerotic heart disease can effectively reduce serum LDL levels, improve heart rate variability, significantly improve the treatment effect.

Objective To study curative efficacy of benazepril combined with atorvastatin on N-terminal pro-brain natriuretic peptide (NT-proBNP) and its efficacy in the treatment of chronic cardiac failure. Methods 90 patients of chronic heart failure were selected as research objects. The control group were treated with benazepril, while the observation group were treated with benazepril combined with atorvastatin, 45 cases in each group. Then before and after treatment of 6 months, the cardiac function of left ventricular end-diastolic dimension (LVEDD), left ventricular end systolic dimension (LVESD), left ventricular eject fraction (LVEF), C-reactionprotein (CRP), interleukin- 6 (IL-6, tumor necrosis factor α (TNF-α) and plasma NT-proBNP levels were compared between two groups, and the efficacy was observed. Results After treatment, LVEDD and LVESD in observation group were lower than those in control group, the LVEF was higher than that in control group(P<0.05). The CRP,IL-6 and TNF-αlevels in observation group were lower than those in control group(P<0.05). The plasma NT-proBNP level in observation group was significantly lower than that in control group(P<0.05). The total effective rate of observation group was statistically higher than that that in the control group 95.55%(43/45)vs. 77.77%(35/45)(P<0.05). Conclusion Benazepril combined with atorvastatin in the treatment of chronic heart failure is significant, can reduce plasma NT-proBNP levels, improve the inflammatory factor.

Objective To study the protective effect and molecular mechanism of trypsin inhibitor on reperfusion injury after cerebral ischemia. Methods The model of ischemia for 1h and repufusion for 24h of rat cerebrum was set by ligating MCAO described by zea-longa. 24 male rats were divided randomly into the sham operation group, the control group and trypsin inhibitor group. The presence of neurological function deficit was measured by Zea-Longa method, and the immunohistochemical staining and TUNEL were used to detect p53 protein expression and cell apoptosis in the brain tissues respectively. Results The score of neurological function deficit was zero in the sham operation group, 2 8?1 0 in the control group and 1 3?0 7 in trypsin inhibitor group. There were 12 3?2 5 p53 immunostaining positive cells in the control group and 5 5?1 3 in trypsin inhibitor group. The number of apoptotic cells was zero in the sham operation group, 7 6?1 0 in the control group, and 3 5?0 9 in trypsin inhibitor group. There was a significant difference in all above observing indices between the control group and trypsin inhibitor group(P

Field practice teaching is an important part in pharmaceutical botany. According to the problems in the traditional field practice, an innovative system of field practice teaching was built. A series of reform and innovation was integrating and rationally allocating the existing resources. Combining the tra-ditional cognitive practice teaching and self-study, image-text card of medicinal plant was edited to enrich the teaching content, and the diversification of evaluation methods was proposed. This method could mobi-lize the enthusiasm of the students, improve the comprehensive quality of students and obtain good teaching effect.

Objective: To explore the prevalence of depression in junior school students and its relationship with potential psychological and environmental factors. Method:Depressive symptoms, self-esteem, life events, social support, family cohesion and conflict were investigated with self-report questionnaire in 300 junior school students, the relationship between depression and other psychological or environmental factors were analyzed through stepwise regression. Results:The prevalence of depression in junior high school students was 11%. Among the 5 investigated psychological and environmental factors, self-esteem and social support were negatively related with depression (standardized regression coefficient were -0.59 and -0.14), while life events positively related with it(standardized regression coefficient was 0.26). Only "academic pressure" and another factors covering the item "do not want to go to school" showed a negative association with the students' self-esteem among the 6 factors included in the life events questionnaire (standardized regression coefficient were -0.149 and -0.13).Conclusion: Depression is common among junior school students. Poor self-esteem, lack of social support and negative life events are currently related with the development of adolescent depression. The pressure and failing experiences coming from academic activities are the most influential life events to the development of adolescents' self-esteem.

Objective To investigate on out the deformity in the elderly of longevous area and the characteristics of the regions in Yunnan Province. Methods According to the forth census information, using multistage sample typical survey to the 18 counties (cities) with centenarians over 1/100 000. The deformity types of people aged 60 and over and the deformity rate of various age groups and its distribution were investigated. Results Among the 6 477 people aged 60 and over investigated, a total of 1 928 people were in deformity, the deformity rate was 29 8%. The elder the people, the higher the deformity rate was. The highest deformity rate was in Kunming and Chuxiong prefectures. The audition deformity was highest among all types of deformity in elder people. The occurrence of fracture was highest in Miao Nation in Honghe prefecture, reaching 18.1%. About 14 % of the centenarian people sufferred from dementia. Conclusions Effective control in blood pressure and osteoporosis are important method to protect the elder people and to promote the level of quality of life of the elderly.

Objective To investigate the risk factors of postoperative delirium in elderly patients with intertrochanteric facture.Methods This retrospective analysis was conducted on 256 patients aged over 65 years treated with internal fixation of intertrochanteric facture between January 2012 and December 2013.Incidence and risk factors of postoperative delirium were documented.Results Incidence of postoperative delirium was 14.8％ (38/256).Risk factors of postoperative delirium included body mass index (BMI) less than 20 kg/m2 (P ＜ 0.05),preoperative hemoglobin (Hb) less than 100 g/L (P ＜ 0.05),atrial fibrillation (P ＜ 0.05),low sodium (P ＜ 0.05),preoperative total protein less than 60 g/L (P ＜ 0.05),preoperative waiting time more than 4 days (P ＜ 0.05),preoperative cognitive impairment (P ＜ 0.05),operation time more than 2.5 hours (P ＜ 0.05),perioperation blood loss more than 1,000 ml (P ＜ 0.05),and more than two kinds of medical conditions (P ＜ 0.05).Logistic regression identified three independent factors for postoperative delirium:preoperative waiting time more than 4 days (P ＜ 0.05,OR =2.74),BMI less than 20 kg/m2 (P ＜ 0.05,OR =13.27),preoperative cognitive impairment (P ＜ 0.05,OR =4.47),and operation time more than 2.5 hours (P ＜ 0.05,OR =4.56).Conclusions Risk factors of postoperative delirium in elder patients with femoral intertrochanteric fracture are complicated.Therefore,overall situation should be taken into consideration and comprehensive and effective treatment should be formulated to prevent the occurrence of postoperative delirium.