ObjectiveTo investigate the effect of Jiawei Glutinous decoction on gouty nephropathy. Methods54 patients with gouty nephropathy were randomized into two groups, 31 patients in the treated group were treated with Jiawei Glutinous decoction, and 23 patients in the control group were treated with allopurinol. Blood uricacid (BUA), renal function, β2-microglobulin (β2-MG) and 24 h urine protein of patiens in two groups were measured before and after treatment.ResultsImprovements of symptoms and renal function as well as decreasing of BUA, β2-MG and 24h PRO in the treated group was superior to those in the control(P<0.05).ConclusionJiawei Glutinous decoction present good effect in treating gouty nephropathy.

Objective To observe heterotopic osteogenesis of autogenous marrow stromal cells on ?-tricalcium phosphate (?-TCP) scaffold, and the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) and transforming growth factor-beta (TGF-?) on osteogenesis. Methods The Newzealand rabbits autogenous marrow stromal cells were cultured by mineralized condition medium. The cell suspension was collected. All the cells with concentration of 5 ?106 ? mL-1 were divided into four groups. Group A: rhBMP-2 (15 ?g) /TGF-? (30 ng) were added; group B: TGF-? 30 ng was administered; group C: without growth factor as control; group D: continual cultured and no loading on ?-TCP. MSCs in group A, B, C were planted on ?-TCP. Alkaline phosphatase (ALP) activity was examined by histochemistry. Then MSCs loading on ?-TCP were embedded in autogenous subcutaneous. Four weeks later, the osteogenesis was observed by HE staining, type I collagen and bone morphogenetic protein synthesis were examined by immunohistochemistry stains. Results The ALP activities in groups A and B were significantly higher than those in group C and D (P

Objective To observe arthroscopic manifestations of necrosis of the femoral head and to evaluate the results. Methods Arthroscopic operations were performed in 38 hips in 27 patients (19 male and 8 female) with necrosis of the femoral head. There were 8 hips of Ficat stage Ⅰ, 21 hips of stage Ⅱ, 7 hips of stage Ⅲ, and 2 hips of stage Ⅳ, respectively. The arthroscopic operation included an assessment of the appearance and texture of the femoral head, the acetabulum, and the synovium, debridement of the joint, and femoral head decompression by drilling small holes. Results Arthroscopically, the Ficat stage I hips were considered to have a intact articular surface, with normal appearance and instrument compression, displaying a hyperaemia and swelling of the synovial tissue. The stage Ⅱ hips presented a major portion of the normal articular surface, chondromalacia of the weight bearing portion of the femoral head, fissures on the articular surface, and sinking with instrument compression. A cleavage plane was formed between the fractured and collapsed necrotic subchondral bone, but the cartilage still communicated with the calcificated layer. The surface of necrotic bone was sclerosesed and rough like sandstones. Hyperplasia of the synovial tissue was seen. The stage Ⅲ hips manifested a massive disassociation of the articular surface from the underlying subchondral bone and the delamination of the femoral head. The subchondral bone was collapsed and disintegrated, with ineffective repaired granulation tissue in it. The stage Ⅳ hips displayed narrowing of the joint space, extensive chondral lesions of the femoral head, collapse of necrotic subchondral bone exposed, and degenerative changes on the acetabulum side, all of which were consistent with the end-stage of osteoarthritis. Conclusions Articular cartilage impairment plays an important role in the process of the necrosis of the femoral head. The value of hip arthroscopic examinations lies in accurate evaluation of the extent of the chondral impairment and important reference for the choice of procedures.

Objective To investigate the indoor air formaldehyde pollution in the human anatomy laboratory and to study the control measures and their effects on prevention and control of formaldehyde pollution. Methods The levels of formaldehyde in the air of the human anatomy laboratory with exhaust ventilatary conditional system and without such a system were determined respectively according to the method recommended in the Sanitary Standard of Public Place. Results Without the exhaust ventilatory conditional system, the level of formaldehyde of indoor air reached to 8.349 mg/m3, while the level of formaldehyde was decreased significantly to 0.501 mg/m3 with the system. Conclusion Without the exhaust ventilatory conditional system, the formaldehyde pollution in the air of the human anatomy laboratory was very serious, which might have adverse effects on teachers' and students' health, while with the system, the formaldehyde pollution could be controlled effectively.

Objective To investigate the relationship of character of atherosclerotic plaque in carotid artery and serum CRP level. Methods The atherosclerotic plaques in carotid arteries were confirmed by multislice CT angiography(MSCTA),in which serum CRP level were detected in 28 patients with cerebral thrombosis,24 patients with lacunar infarction and 19 patients with TIA in internal carotid artery system in order to analysis the relationship between character of plaque and serum CRP level. Results (1)Mixed plaque and soft plaque were mainly found in cerebral thrombosis and TIA groups, while rigidity plaque was mostly found in lacunar infarction group, there were significance difference in plaques distribution among three groups(all P0.05).(3) CRP level in different groups was (4.546?2.720)mg/L in soft plaque group, (3.951?1.863)mg/L in mixed plaque group respectively,those were both higher than that of the rigidity plaque group[(2.762?1.323)mg/L],and there was significance difference between them(all P

Objective To investigate the value of 18F-FDG PET/CT for differentiating multiple myeloma from bone metastases with undetermined origin.Methods A retrospective study was performed on patients with multiple bone destructions and without extraosseous primary malignant tumor in 18F-FDG PET/CT imaging.A total of 26 patients (12 males,average age 55.4 years) with multiple myeloma and 20 patients (9 males,average age 50.2 years) with multiple bone metastases confirmed by biopsy or histopathology from January 2011 to December 2013 were included into this study.The characteristics of 18F-FDG PET/CT and urine Bence-Jones protein were used to establish the diagnostic criteria.Myeloma was diagnosed if two or more of the following conditions could be satisfied:more than 10 bone lesions,osteolytic or mixed bone destruction,SUVmax＜4.0,and positive urine Bence-Jones protein.Two-sample t test and x2 test were used.Results The average number of bone lesions in multiple myeloma patients was 15.8,which was more than that (7.8) in patients with bone metastases.About 71.8％ (296/412) of bone lesions in multiple myeloma and 28.8％ (45/156) of bone metastases were osteolytic.The percentage of osteolytic lesions was significantly higher in multiple myeloma (x2=87.2,P＜0.05).The SUVmax of bone lesions of multiple myeloma and bone metastases were 3.81 ±2.17 and 5.82± 3.44 (t =8.29,P＜0.05) respectively.According to the diagnostic criteria,the sensitivity,specificity and accuracy of 18F-FDG PET/CT for the differentiation of myeloma from bone metastases were 88.5％ (23/26),85.0％ (17/20) and 87.0％ (40/46).Its diagnostic efficiency was higher than urine Bence-Jones protein (sensitivity:65.4％ (17/26);x2=3.90) and original 18 F-FDG PET/CT evaluation (specificity:10/18,accuracy:64.1％ (25/39);x2 =3.99,6.12) respectively (all P＜0.05).Conclusion Combining the evaluation of structural properties and metabolism on 18F-FDG PET/CT with urine Bence-Jones protein level may provide additional value for the differentiation of multiple myeloma from bone metastases with undetermined origin.

Objective To separate the CD133 + subpopulation in human hepatocellular carcinoma (HCC) and investigate the tumorigenicity.Methods The human liver cancer tissues were subcutaneously transplanted into nude mice to generate xenograft tumors which were then isolated to prepare single cell suspension.The expression of CD133 + subpopulation was further detected using flow cytometry.The CD133 + subpopulations were separated and depurated with magnetic-activated cell sorting system.Immunofluorescence was performed to identify the histological phenotype of CD133 + subpopulation.The in vitro and in vivo clone formation assay and in vivo xenograft formation assay were performed, respectively.Results Flow cytometry analysis revealed that a percentage of (4.1 ± 0.6) ％ CD133 + cells were detected in xenografts.Immunofluorescence studies showed that (86.8 ± 7.5) ％ of the isolated cells were CD133 +.Compared with CD133-population, CD133 + cells showed a higher capability to generate clone sphere in vitro and a higher tumorigenicity in nude mice (P ＜ 0.05).Conclusion The CD133 + subpopulation in human hepatocellular carcinoma had a potent tumorigenicity and was enriched in cancer stem cells.

Objective To explore the optimum exposure factors in digital chest radiography.Methods Chest phantom was exposed under auto exposure control model with 73, 90, 125kV and S200,400,800 plus or minor 4 micro-adjust for each sensitivity grade. Meanwhile dose area product (DAP) was recorded and the value of IQFinv was analyzed automatically by Artinis CDRAD Analyzer 1.1. Ten volunteers were exposed with 73 kV, S800 - 2; 90 kV, S800 + 2 and 125 kV, S400 + 2. Two radiologists evaluated and scored image quality. Statistical analysis was performed using one way ANOVA test by SPSS 12. 0. Results ( 1 ) The quality scores of volunteers' images obtained with three combinations of exposure factors were 2. 7 ± 0. 5 for 73 kV group, 2. 9 ± 0. 3 for 90 kV group and 2. 8 ± 0. 4 for 125 kV group. The difference among them was not statistically significant ( F = 0. 587, P ＞ 0. 05 ). Whereas the DAP values were (29. 1 ± 7.9) mGy · cm2 for 73 kV group, ( 30. 5 ± 4. 5 ) mGy ·cm2 for 90 kV group and (40. 4 ±7.6) mGy · cm2 for 125 kV group, with statistically significant difference among them ( F = 9. 803, P ＜0. 01 ). (2) In all three kV conditions, DAP value of phantom declined when sensitivity increased. There was a difference of DAP value by 11％ between two successive sensitivity grades. Under the condition of same sensitivity, DAP value changed with kV in the following order: 73 kV ＞90 kV ＞ 125 kV. (3) The value of IQFinv decreased when sensitivity increased. Under the condition of same sensitivity, IQFinv changed with kV as follow: 73 kV ＞90 kV ＞ 125 kV. Conclusion The combination of exposure factors of 90 kV and S800 + 2-S800 +4 is optimum for digital chest radiography.

Objective To evaluate treatment outcomes and quality of life (QOL) in glioma patients treated with postoperative intensity-modulated radiotherapy (IMRT), and to explore the possible clinical factors of affecting QOL. Methods From 2007 to 2009, 37 patients with low or high grade glioma were analyzed retrospectively. All patients were operated by tumor resection below microscopy. IMRT began at 2-4 week postoperstion with 2.0 Gy/fractior, 5 fractions/week and to shrink portal and to add dose to 50-60 Gy/25-30 fractions after 40-50 Gy. The gross tumor volume (GTV) was defined as preoperation T2WI MRI high sign area and postoperation tumor cavity for low grade glioma, and with preoperation T1WI MRI enhanced abnormity area and postoperation tumor cavity for high grade glioma. The clinical target volume ( CTV ) was defined as GTV with a margin of 1.5 cm for low grade glioma and a margin of 2.5 cm for high grade ghoma, the planning target volume (PTV) with CTV plus 0.4 cm margin for setup errors according to the European Organization for Research and Treatment of Cancer ( EORTC ).The treatment outcomes and QOL were assessed. Results The half-year and one-year survival rates for all the patients were 100% and 79.2%, respectively. The median progression-free survival time was 10 months. The main side-responses after postoperative IMRT were fatigue and mild memory decline or cognitive disabilities, which were radiation dose-dependent. Conclusions Postoperative IMRT is an effective and safe modality of therapy for glioma patients.

Objective To compare the effects of three kinds of fixative solutions on paraffin section of mouse lens tissue and optimize the fixing?method of paraffin section in mouse lens tissue. Methods Three kinds of conventional fixatives were selected for the test ,including the conven?tional Davison’s solution,modified Davison’s solution and 10%neutral buffered formalin. Mice eyeball tissues were fixed with three different fixa?tives,embedded,sliced and then stained with HE method. The paraffin slices were observed under the light microscope. Results The structures of lens and retina fixed in conventional Davison ’s fixative solutions were clear and intact ,and the cells were arranged regularly and compactly. There was no eyeball distortion,contraction and retinal detachment in the eyeballs fixed in modified and conventional Davison’s fixative solution. However,the ones fixed in 10%neutral buffered formalin showed eyeball distortion and contraction,space and spherules. Conclusion The mice lens slides made from tissues fixed by conventional Davison ’s fixative solution are better than fixed by modified Davison ’s fixative solution and the 10%neutral buffered formalin fixed ones.