To investigate the effects of ligustrazine injection on type I collagen, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) expressions in human peritoneal mesothelial cells (HPMCs) cultured in high glucose conditions.

Objective To investigate the effects of Ligustrazine on TNF-α-induced TGF-β and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin di-gestion. Then, the subcultured HPMCs were divided into control group, TNF-α-induced (1 μg/L) group and TNF-α-induced plus low-, medium-and high-dose Ligustrazine (10, 20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-β1 and CTGF mRNAs in HPMCs. TGF-β1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results. Results Ligustrazine significantly decreases TNF-α-induced TGF-β1 and CTGF expression in a dose-dependent manner at both protein and gene levels ( P < 0. 05 ). In addition, medium-and high-dose Ligustrazine injection significantly ameliorates the viability of HPMCs inhibited by TNF-α ( P < 0. 05 ). Conclusion Ligustrazine inhibits expressions of TGF-β and CTGF of HPMCs in an inflammatory conditions.

Objective To investigate the effects of Ligustrazine on TNF-?-induced TGF-?1 and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin digestion. Then,the subcultured HPMCs were divided into control group,TNF-?-induced (1 ?g/L) group and TNF-?-induced plus low-,medium-and high-dose Ligustrazine (10,20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-?1 and CTGF mRNAs in HPMCs. TGF-?1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results.ResultsLigustrazine significantly decreases TNF-?-induced TGF-?1 and CTGF expression in a dose-dependent manner at both protein and gene levels (P

Theprospectofliverdiseasetreatmentbyhepatocytetransplantationisbroad ,butitis difficulttoapplyitinclinictherapyduetotherestrictionofsourceandproliferationofdonorhepatocyte .The hematopoieticstemcellplasticityoftrans -differentiationtohepatocyteprovidesanewsourceofseedcellsfor hepatocytetransplantation .Inthisreview ,wefocusonadvancesintheseedcellsforhepatocytetransplanta tion .

Objective To summary the results of fixation and fusion with lateral mass screw of atlas and vertebra dentata pedicle screw for the treatment of atlantoaxial instability. Methods From February 2002 to March 2004, 15 cases suffered with atlantoaxial instability were included in this study. There were 9 males and 6 females, with a mean age of 39.5 years old(ranged 15 to 57 years old). There were 5 cases for old odontoid fracture, 4 cases for congenital loose odontoid process, 6 cases for fresh odontoid fracture(Aderson ⅡC). 7 cases were fixed with Vertex, 3 Axis and 5 Cervifix instruments. JOA scores in preoperation were from 5.1 to 10.9, with an average of 7.6. Skull traction was performed in each patient preoperatively. The screws were placed in lateral mass of atlas beside the posterior arch of atlas about 18-20 mm and the point of intersection above 2 mm inferior boarder of posterior arch. Vertical with coronal plane, the tip of the screw made 5? to side of head in sagittal plane. The site of the screws were placed in vertebra dentate was divide equally the inferior articular process of axis, made 15? with sagittal plane and 30? with cross section. The diameter of the screw was 3.5 mm, the length of in lateral mass of atlas was from 28 to 32 mm, and from 22 to 26 mm in vertebra dentate pedicle screw. Results There were no spinal cord and vertebral artery injury after surgery. Follow-up duration was from 10 to 25 months, with average 14 months. The postoperative JOA scores were from 13.2 to 16.8, with average 14.8. The improve rate was 87.5 percent. The plant bones all fused and there were no internal fixation rupture and mobiled. Conclusion Lateral mass screw of atlas and vertebra dentata pedicle screw with three-dimension fixed virtues could be used to treat atlantoaxial instability.

Objective To evaluate the clinical application of high-frequency ultrasound-guided Mammotome Breast Biopsy System in the biopsy of breast microcalcification.Methods The sterotactic biopsy of microcalcification in breast was performed under the guidance of high-frequency ultrasonography in 24 patients.Results The location of the lesion was accurately determined in all the 24 patients.Pathological findings showed malignant breast tumors in 3 patients(12.5%) and benign breast diseases in 21 patients(87.5%).The malignant tumors included ductal carcinoma in situ in 2 patients and invasive ductal carcinoma in 1 patient.The benign diseases included cystic lobular hyperplasia in 9 patients,ductal hyperplasia and dilation in 6 patients,galactophore cirrhosis in 5 patients,and intraductal papilloma in 1 patient. Conclusions Use of the Mammotome Breast Biopsy System under the guidance of high-frequency ultrasonography is a safe and effective option for the diagnosis of impalpable breast lesions.

Objective To estimate the test-retest reliability of limits of stability (LOS) measurement in healthy young adults. MethodsTwenty-five healthy young adults participated this study.Their center of pressure (COP) displacements were recorded while they stood on a force platform.The subjects performed maximal voluntary sway maneuvers in the anterior,posterior,left and right directions in turn.The LOS in each direction was calculated using balance clinic software.Each subject underwent 3 consecutive,identical tests at 7 day intervals with the same investigator.The test-retest reliability of LOS was analyzed using the intraclass correlation coefficient (ICC).ResultsFor the first test the ICC values ( ICC1 ) of the LOS ranged from 0.790 to 0.857.For the mean LOS of the first two tests the ICC2 ranged from 0.906 to 0.937,and for the mean LOS of three tests the ICC3 ranged from 0.910 to 0.948. ConclusionThe LOS test is a reliable measurement with healthy young adults.In clinical practice,averaging two LOS tests is suggested in assessing balance and postural control with young adults.

0.05). The incidence of cardiovascular event in both groups was significantly different (P

Objective To evaluate the quality of observational studies on pelvic organ prolapse in China.Methods The checklist of strengthening the reporting of observational studies in epidemiology (STROBE) statement was applied to evaluate the observational studies.The articles were searched in the SinoMed database using the terms:prolapse,uterine prolapse,cystocele,rectal prolapse and pelvic floor;limited to Chinese core journals in obstetrics and gynecology from January 1996 to December 2015.With two 10-year groups (1996-2005 and 2006-2015),the x2 test was used to evaluate inter-group differences.Results (1) A total of 386 observational studies were selected,including 15.5％(60/386) of case-control studies,80.6％(311/386) of cohort studies and 3.9％ (15/386) of cross-sectional studies.(2) There were totally 22 items including 34 sub-items in the checklist.There were 17 sub-items (50.0％,17/34) had a reporting ratio less than 50％ in all of aticles,including:1a (study's design) 3.9％ (15/386),6a (participants) 24.6％(95/386),6b (matched studies) 0 (0/386),9 (bias) 8.3％ (32/386),10 (study size) 3.9％,11 (quantitative variables) 41.2％ (159/386),12b-12e (statistical methods in detail) 0-2.6％ (10/386),13a (numbers of individuals at each stage of study) 18.9％ (73/386),13b (reasons for non-participation at each stage) 18.9％,13c (flow diagram) 0,16b and 16c (results of category boundaries and relative risk) 9.6％ (37/386) and 0,19 (limitations) 31.6％ (122/386),22 (funding) 20.5％ (79/386).(3) The quality of articles published in the two decades (1996-2005 and 2006-2015) were compared,and 38.2％ (13/34) of sub-items had been significantly improved in the second 10-year (all P＜0.05).The improved items were as follows:1b (integrity of abstract),2 (background/rationale),6a (participants),7 (variables),8 (data sources/measurement),9 (bias),11 (quantitative variables),12a (statistical methods),17 (other analyses),18 (key results),19 (limitations),21 (generalisability),22 (funding).Conclusions The quality of observational studies on POP in China is suboptimal in half of evaluation items.However,the quality of articles published in the second 10-year have significantly improved.

Objective:The non targeted high-throughput urine metabolomics technology was used to study the pathogenesis of APP/PS 1 double transgenic mice and the mechanism of action of Gouteng san.Methods:5-month-old APP/PS 1 double transgenic mice were test with Morris water maze for spatial learning ability.Then we employed the non targeted high-throughput urine metabolomics technology to study the pathogenesis of APP/PS1 double transgenic mice based on the metabolic network.The focus investigation of the key pathways and the observation of the treatment by Morris water maze and metabolic level have been used after spatial learning ability damaged confirmed.Results:The comparison between APP/PS1 double transgenic mice and normal mice suggested that a significant longer was existed in former,which was call-back by Gouteng san.With the non targeted high-throughput urine metabolomics analysis and pathway focused analysis,we found certain signals from metabolic profiling,which was identified to be 6 biomarkers associated with learning and memory function by mass spectrometry analysis or authoritative database.Respectively,they were taurine,pteroylglutamic acid,neopterin,glutaurine,2-oxoglutarate and dihydroneopterin.They were mainly related to taurine metabolism and folate metabolism and represented an effective callback.Conclusion:Gouteng san possess a favorable effect on learning and memory ability of APP/PS1 double transgenic mice,6 biomarkers may be a potential target for the pathogenesis of APP/PSI double transgenic mice and provide experimental basis for the study of Gouteng san.